Molecular cloning of Brugia malayi antigens for diagnosis of lymphatic filariasis.
Identifieur interne : 005572 ( PubMed/Corpus ); précédent : 005571; suivant : 005573Molecular cloning of Brugia malayi antigens for diagnosis of lymphatic filariasis.
Auteurs : R. Chandrashekar ; K C Curtis ; R M Ramzy ; F. Liftis ; B W Li ; G J WeilSource :
- Molecular and biochemical parasitology [ 0166-6851 ] ; 1994.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (genetics), Brugia malayi (genetics), Brugia malayi (immunology), Cloning, Molecular, DNA, Helminth (genetics), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay (statistics & numerical data), Genetic Vectors, Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Immunoglobulin G (blood), Immunoglobulin G (classification), Immunologic Tests, Molecular Sequence Data, Sensitivity and Specificity, Sequence Homology, Amino Acid.
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- chemical , classification : Immunoglobulin G.
- chemical , genetics : Antigens, Helminth, DNA, Helminth, Helminth Proteins.
- diagnosis : Elephantiasis, Filarial.
- genetics : Brugia malayi.
- immunology : Brugia malayi, Elephantiasis, Filarial, Helminth Proteins.
- statistics & numerical data : Enzyme-Linked Immunosorbent Assay.
- Amino Acid Sequence, Animals, Cloning, Molecular, Genetic Vectors, Humans, Immunologic Tests, Molecular Sequence Data, Sensitivity and Specificity, Sequence Homology, Amino Acid.
Abstract
Immunological crossreactivity among nematodes has hampered development of specific serodiagnostic assays for lymphatic filariasis. In the present study, we report the molecular cloning and characterization of two filaria-specific recombinant clones (BmM5 and BmM14) with immunodiagnostic potential. BmM5 is a 505-bp cDNA which codes for a protein of 130 residues that ends with an endoplasmic reticulum targeting sequence. BmM14 is closely related to a recently reported clone (SXP-1), and it has 62% homology (deduced amino acid sequence) with a previously described Onchocerca volvulus clone, lambda RAL-2. Glutathione S-transferase fusion proteins of BmM5 and BmM14 were tested in various ELISA formats. The best results were obtained by measuring IgG4 antibodies to the fusion proteins. ELISA studies showed that approximately 90% of 111 sera from Indian and Egyptian patients with brugian and bancroftian filariasis were reactive with both antigens. Nonendemic sera as well as sera from patients with schistosomiasis or intestinal helminths were uniformly nonreactive. Assays based on BmM5 and BmM14 may be useful for large scale screening as an alternative to microfilaria or filarial antigen detection as a means of obtaining a rough index of filariasis endemicity in previously unstudied areas.
PubMed: 7935604
Links to Exploration step
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning of Brugia malayi antigens for diagnosis of lymphatic filariasis.</title><author><name sortKey="Chandrashekar, R" sort="Chandrashekar, R" uniqKey="Chandrashekar R" first="R" last="Chandrashekar">R. Chandrashekar</name><affiliation><nlm:affiliation>Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.</nlm:affiliation></affiliation></author><author><name sortKey="Curtis, K C" sort="Curtis, K C" uniqKey="Curtis K" first="K C" last="Curtis">K C Curtis</name></author><author><name sortKey="Ramzy, R M" sort="Ramzy, R M" uniqKey="Ramzy R" first="R M" last="Ramzy">R M Ramzy</name></author><author><name sortKey="Liftis, F" sort="Liftis, F" uniqKey="Liftis F" first="F" last="Liftis">F. Liftis</name></author><author><name sortKey="Li, B W" sort="Li, B W" uniqKey="Li B" first="B W" last="Li">B W Li</name></author><author><name sortKey="Weil, G J" sort="Weil, G J" uniqKey="Weil G" first="G J" last="Weil">G J Weil</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1994">1994</date><idno type="RBID">pubmed:7935604</idno><idno type="pmid">7935604</idno><idno type="wicri:Area/PubMed/Corpus">005572</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005572</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning of Brugia malayi antigens for diagnosis of lymphatic filariasis.</title><author><name sortKey="Chandrashekar, R" sort="Chandrashekar, R" uniqKey="Chandrashekar R" first="R" last="Chandrashekar">R. Chandrashekar</name><affiliation><nlm:affiliation>Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.</nlm:affiliation></affiliation></author><author><name sortKey="Curtis, K C" sort="Curtis, K C" uniqKey="Curtis K" first="K C" last="Curtis">K C Curtis</name></author><author><name sortKey="Ramzy, R M" sort="Ramzy, R M" uniqKey="Ramzy R" first="R M" last="Ramzy">R M Ramzy</name></author><author><name sortKey="Liftis, F" sort="Liftis, F" uniqKey="Liftis F" first="F" last="Liftis">F. Liftis</name></author><author><name sortKey="Li, B W" sort="Li, B W" uniqKey="Li B" first="B W" last="Li">B W Li</name></author><author><name sortKey="Weil, G J" sort="Weil, G J" uniqKey="Weil G" first="G J" last="Weil">G J Weil</name></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="ISSN">0166-6851</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (immunology)</term><term>Cloning, Molecular</term><term>DNA, Helminth (genetics)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay (statistics & numerical data)</term><term>Genetic Vectors</term><term>Helminth Proteins (genetics)</term><term>Helminth Proteins (immunology)</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Immunoglobulin G (classification)</term><term>Immunologic Tests</term><term>Molecular Sequence Data</term><term>Sensitivity and Specificity</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>DNA, Helminth</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>Genetic Vectors</term><term>Humans</term><term>Immunologic Tests</term><term>Molecular Sequence Data</term><term>Sensitivity and Specificity</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Immunological crossreactivity among nematodes has hampered development of specific serodiagnostic assays for lymphatic filariasis. In the present study, we report the molecular cloning and characterization of two filaria-specific recombinant clones (BmM5 and BmM14) with immunodiagnostic potential. BmM5 is a 505-bp cDNA which codes for a protein of 130 residues that ends with an endoplasmic reticulum targeting sequence. BmM14 is closely related to a recently reported clone (SXP-1), and it has 62% homology (deduced amino acid sequence) with a previously described Onchocerca volvulus clone, lambda RAL-2. Glutathione S-transferase fusion proteins of BmM5 and BmM14 were tested in various ELISA formats. The best results were obtained by measuring IgG4 antibodies to the fusion proteins. ELISA studies showed that approximately 90% of 111 sera from Indian and Egyptian patients with brugian and bancroftian filariasis were reactive with both antigens. Nonendemic sera as well as sera from patients with schistosomiasis or intestinal helminths were uniformly nonreactive. Assays based on BmM5 and BmM14 may be useful for large scale screening as an alternative to microfilaria or filarial antigen detection as a means of obtaining a rough index of filariasis endemicity in previously unstudied areas.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7935604</PMID><DateCreated><Year>1994</Year><Month>11</Month><Day>09</Day></DateCreated><DateCompleted><Year>1994</Year><Month>11</Month><Day>09</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-6851</ISSN><JournalIssue CitedMedium="Print"><Volume>64</Volume><Issue>2</Issue><PubDate><Year>1994</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Molecular and biochemical parasitology</Title><ISOAbbreviation>Mol. Biochem. Parasitol.</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning of Brugia malayi antigens for diagnosis of lymphatic filariasis.</ArticleTitle><Pagination><MedlinePgn>261-71</MedlinePgn></Pagination><Abstract><AbstractText>Immunological crossreactivity among nematodes has hampered development of specific serodiagnostic assays for lymphatic filariasis. In the present study, we report the molecular cloning and characterization of two filaria-specific recombinant clones (BmM5 and BmM14) with immunodiagnostic potential. BmM5 is a 505-bp cDNA which codes for a protein of 130 residues that ends with an endoplasmic reticulum targeting sequence. BmM14 is closely related to a recently reported clone (SXP-1), and it has 62% homology (deduced amino acid sequence) with a previously described Onchocerca volvulus clone, lambda RAL-2. Glutathione S-transferase fusion proteins of BmM5 and BmM14 were tested in various ELISA formats. The best results were obtained by measuring IgG4 antibodies to the fusion proteins. ELISA studies showed that approximately 90% of 111 sera from Indian and Egyptian patients with brugian and bancroftian filariasis were reactive with both antigens. Nonendemic sera as well as sera from patients with schistosomiasis or intestinal helminths were uniformly nonreactive. Assays based on BmM5 and BmM14 may be useful for large scale screening as an alternative to microfilaria or filarial antigen detection as a means of obtaining a rough index of filariasis endemicity in previously unstudied areas.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chandrashekar</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Curtis</LastName><ForeName>K C</ForeName><Initials>KC</Initials></Author><Author ValidYN="Y"><LastName>Ramzy</LastName><ForeName>R M</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Liftis</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>B W</ForeName><Initials>BW</Initials></Author><Author ValidYN="Y"><LastName>Weil</LastName><ForeName>G 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MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018509" MajorTopicYN="N">DNA, Helminth</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" 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