Biased TCR repertoire in infiltrating lesional T cells in human Bancroftian filariasis.
Identifieur interne : 004D24 ( PubMed/Corpus ); précédent : 004D23; suivant : 004D25Biased TCR repertoire in infiltrating lesional T cells in human Bancroftian filariasis.
Auteurs : D O Freedman ; D A Plier ; A. De Almeida ; J. Miranda ; C. Braga ; M C Maia E Silva ; J. Tang ; A. FurtadoSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1999.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cytokines (genetics), DNA Primers (genetics), Elephantiasis, Filarial (etiology), Elephantiasis, Filarial (genetics), Elephantiasis, Filarial (immunology), Gene Expression, HLA-DR Antigens (genetics), HLA-DRB1 Chains, Humans, RNA, Messenger (genetics), Receptors, Antigen, T-Cell, alpha-beta (genetics), Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes (immunology), Wuchereria bancrofti.
- MESH :
- chemical , genetics : Cytokines, DNA Primers, HLA-DR Antigens, RNA, Messenger, Receptors, Antigen, T-Cell, alpha-beta.
- etiology : Elephantiasis, Filarial.
- genetics : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, T-Lymphocytes.
- Animals, Base Sequence, Gene Expression, HLA-DRB1 Chains, Humans, Reverse Transcriptase Polymerase Chain Reaction, Wuchereria bancrofti.
Abstract
To investigate the hypothesis that T cells recognizing specific Ags localize to the site of disease activity in human bancroftian filariasis, we have compared the repertoire of TCR Vbeta gene segments in lesions vs blood in individual patients by RT-PCR ELISA. Vbeta14 and Vbeta24 were overrepresented (5% greater in tissue compared with PBMCs and/or tissue/PBMC ratios in the highest 5% of all tissue/PBMC ratios for all Vbetas for all subjects) in 50% and 40% of study subjects, respectively. Overrepresentation of these two Vbetas did not occur in any control subject. In comparing three patient groups, the proportion of individuals meeting at least one criterion for Vbeta14 overrepresentation was shown to increase in tandem with our current concepts of disease progression (asymptomatic filariasis = 25%; clinical filariasis with active infection = 60%; clinical filariasis without active infection = 71%). In 6 of the 10 individuals with Vbeta14 overrepresentation, Vbeta14 represented >20% of the entire lesional Vbeta repertoire. All but one of the 20 study subjects had at least one Vbeta gene segment that was overrepresented in tissue compared with PBMCs. Only a small number of Vbetas, usually three or less, were overrepresented in any single filariasis patient. However, in the same tissue, no differences between patient groups were found when IFN-gamma, TNF-alpha, IL-4, IL-5, and IL-12 mRNA expression were examined. Taken together, these findings suggest that, in principle, in essentially all patients, whether with subclinical or with clinical filariasis, distinct and limited T cell populations are concentrated in affected tissue.
PubMed: 9973439
Links to Exploration step
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biased TCR repertoire in infiltrating lesional T cells in human Bancroftian filariasis.</title><author><name sortKey="Freedman, D O" sort="Freedman, D O" uniqKey="Freedman D" first="D O" last="Freedman">D O Freedman</name><affiliation><nlm:affiliation>Division of Geographic Medicine, Department of Medicine, University of Alabama, Birmingham, AL 35294, USA. dfreedman@geomed.dom.uab.edu</nlm:affiliation></affiliation></author><author><name sortKey="Plier, D A" sort="Plier, D A" uniqKey="Plier D" first="D A" last="Plier">D A Plier</name></author><author><name sortKey="De Almeida, A" sort="De Almeida, A" uniqKey="De Almeida A" first="A" last="De Almeida">A. De Almeida</name></author><author><name sortKey="Miranda, J" sort="Miranda, J" uniqKey="Miranda J" first="J" last="Miranda">J. Miranda</name></author><author><name sortKey="Braga, C" sort="Braga, C" uniqKey="Braga C" first="C" last="Braga">C. Braga</name></author><author><name sortKey="Maia E Silva, M C" sort="Maia E Silva, M C" uniqKey="Maia E Silva M" first="M C" last="Maia E Silva">M C Maia E Silva</name></author><author><name sortKey="Tang, J" sort="Tang, J" uniqKey="Tang J" first="J" last="Tang">J. Tang</name></author><author><name sortKey="Furtado, A" sort="Furtado, A" uniqKey="Furtado A" first="A" last="Furtado">A. Furtado</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:9973439</idno><idno type="pmid">9973439</idno><idno type="wicri:Area/PubMed/Corpus">004D24</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">004D24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biased TCR repertoire in infiltrating lesional T cells in human Bancroftian filariasis.</title><author><name sortKey="Freedman, D O" sort="Freedman, D O" uniqKey="Freedman D" first="D O" last="Freedman">D O Freedman</name><affiliation><nlm:affiliation>Division of Geographic Medicine, Department of Medicine, University of Alabama, Birmingham, AL 35294, USA. dfreedman@geomed.dom.uab.edu</nlm:affiliation></affiliation></author><author><name sortKey="Plier, D A" sort="Plier, D A" uniqKey="Plier D" first="D A" last="Plier">D A Plier</name></author><author><name sortKey="De Almeida, A" sort="De Almeida, A" uniqKey="De Almeida A" first="A" last="De Almeida">A. De Almeida</name></author><author><name sortKey="Miranda, J" sort="Miranda, J" uniqKey="Miranda J" first="J" last="Miranda">J. Miranda</name></author><author><name sortKey="Braga, C" sort="Braga, C" uniqKey="Braga C" first="C" last="Braga">C. Braga</name></author><author><name sortKey="Maia E Silva, M C" sort="Maia E Silva, M C" uniqKey="Maia E Silva M" first="M C" last="Maia E Silva">M C Maia E Silva</name></author><author><name sortKey="Tang, J" sort="Tang, J" uniqKey="Tang J" first="J" last="Tang">J. Tang</name></author><author><name sortKey="Furtado, A" sort="Furtado, A" uniqKey="Furtado A" first="A" last="Furtado">A. Furtado</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cytokines (genetics)</term><term>DNA Primers (genetics)</term><term>Elephantiasis, Filarial (etiology)</term><term>Elephantiasis, Filarial (genetics)</term><term>Elephantiasis, Filarial (immunology)</term><term>Gene Expression</term><term>HLA-DR Antigens (genetics)</term><term>HLA-DRB1 Chains</term><term>Humans</term><term>RNA, Messenger (genetics)</term><term>Receptors, Antigen, T-Cell, alpha-beta (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>T-Lymphocytes (immunology)</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytokines</term><term>DNA Primers</term><term>HLA-DR Antigens</term><term>RNA, Messenger</term><term>Receptors, Antigen, T-Cell, alpha-beta</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Gene Expression</term><term>HLA-DRB1 Chains</term><term>Humans</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Wuchereria bancrofti</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To investigate the hypothesis that T cells recognizing specific Ags localize to the site of disease activity in human bancroftian filariasis, we have compared the repertoire of TCR Vbeta gene segments in lesions vs blood in individual patients by RT-PCR ELISA. Vbeta14 and Vbeta24 were overrepresented (5% greater in tissue compared with PBMCs and/or tissue/PBMC ratios in the highest 5% of all tissue/PBMC ratios for all Vbetas for all subjects) in 50% and 40% of study subjects, respectively. Overrepresentation of these two Vbetas did not occur in any control subject. In comparing three patient groups, the proportion of individuals meeting at least one criterion for Vbeta14 overrepresentation was shown to increase in tandem with our current concepts of disease progression (asymptomatic filariasis = 25%; clinical filariasis with active infection = 60%; clinical filariasis without active infection = 71%). In 6 of the 10 individuals with Vbeta14 overrepresentation, Vbeta14 represented >20% of the entire lesional Vbeta repertoire. All but one of the 20 study subjects had at least one Vbeta gene segment that was overrepresented in tissue compared with PBMCs. Only a small number of Vbetas, usually three or less, were overrepresented in any single filariasis patient. However, in the same tissue, no differences between patient groups were found when IFN-gamma, TNF-alpha, IL-4, IL-5, and IL-12 mRNA expression were examined. Taken together, these findings suggest that, in principle, in essentially all patients, whether with subclinical or with clinical filariasis, distinct and limited T cell populations are concentrated in affected tissue.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9973439</PMID><DateCreated><Year>1999</Year><Month>04</Month><Day>13</Day></DateCreated><DateCompleted><Year>1999</Year><Month>04</Month><Day>13</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1767</ISSN><JournalIssue CitedMedium="Print"><Volume>162</Volume><Issue>3</Issue><PubDate><Year>1999</Year><Month>Feb</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of immunology (Baltimore, Md. : 1950)</Title><ISOAbbreviation>J. Immunol.</ISOAbbreviation></Journal><ArticleTitle>Biased TCR repertoire in infiltrating lesional T cells in human Bancroftian filariasis.</ArticleTitle><Pagination><MedlinePgn>1756-64</MedlinePgn></Pagination><Abstract><AbstractText>To investigate the hypothesis that T cells recognizing specific Ags localize to the site of disease activity in human bancroftian filariasis, we have compared the repertoire of TCR Vbeta gene segments in lesions vs blood in individual patients by RT-PCR ELISA. Vbeta14 and Vbeta24 were overrepresented (5% greater in tissue compared with PBMCs and/or tissue/PBMC ratios in the highest 5% of all tissue/PBMC ratios for all Vbetas for all subjects) in 50% and 40% of study subjects, respectively. Overrepresentation of these two Vbetas did not occur in any control subject. In comparing three patient groups, the proportion of individuals meeting at least one criterion for Vbeta14 overrepresentation was shown to increase in tandem with our current concepts of disease progression (asymptomatic filariasis = 25%; clinical filariasis with active infection = 60%; clinical filariasis without active infection = 71%). In 6 of the 10 individuals with Vbeta14 overrepresentation, Vbeta14 represented >20% of the entire lesional Vbeta repertoire. All but one of the 20 study subjects had at least one Vbeta gene segment that was overrepresented in tissue compared with PBMCs. Only a small number of Vbetas, usually three or less, were overrepresented in any single filariasis patient. However, in the same tissue, no differences between patient groups were found when IFN-gamma, TNF-alpha, IL-4, IL-5, and IL-12 mRNA expression were examined. Taken together, these findings suggest that, in principle, in essentially all patients, whether with subclinical or with clinical filariasis, distinct and limited T cell populations are concentrated in affected tissue.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Freedman</LastName><ForeName>D O</ForeName><Initials>DO</Initials><AffiliationInfo><Affiliation>Division of Geographic Medicine, Department of Medicine, University of Alabama, Birmingham, AL 35294, USA. dfreedman@geomed.dom.uab.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Plier</LastName><ForeName>D A</ForeName><Initials>DA</Initials></Author><Author ValidYN="Y"><LastName>de Almeida</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Miranda</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Braga</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Maia e Silva</LastName><ForeName>M C</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Furtado</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>AI-31552</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J 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MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000209" MajorTopicYN="N">etiology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006684" MajorTopicYN="N">HLA-DR 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