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Molecular characterisation of the actin gene of the filarial parasite Wuchereria bancrofti.

Identifieur interne : 004B17 ( PubMed/Corpus ); précédent : 004B16; suivant : 004B18

Molecular characterisation of the actin gene of the filarial parasite Wuchereria bancrofti.

Auteurs : J K Casinader Saverimuttu ; E H Karunanayake ; N V Chandrasekharan ; S M Jayasena

Source :

International journal for parasitology [ 0020-7519 ] ; 2000.

RBID : pubmed:10704593

English descriptors

KwdEn :
- Actins (genetics), Amino Acid Sequence, Animals, Base Sequence, Consensus Sequence, Elephantiasis, Filarial (genetics), Elephantiasis, Filarial (parasitology), Gene Library, Humans, Molecular Sequence Data, Restriction Mapping, Sequence Alignment, TATA Box, Wuchereria bancrofti (genetics).
MESH :
- chemical , genetics : Actins.
- genetics : Elephantiasis, Filarial, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Base Sequence, Consensus Sequence, Gene Library, Humans, Molecular Sequence Data, Restriction Mapping, Sequence Alignment, TATA Box.

Abstract

Wuchereria bancrofti is the major cause of lymphatic filariasis in humans. Although it is responsible for this immensely morbid and debilitating disease, very little is known of the basic molecular biology of this parasite, and there is a vast lack of knowledge on its gene organisation. In this study, the actin gene of W. bancrofti has been characterised by sequencing a clone isolated from a genomic DNA library of this parasite. The 5' flanking region had a potential TATA box and a putative mRNA initiation site. The gene had five exons encoding 376 amino acids, and four introns ranging in size from 109 to 190bp. The 3' flanking region had a potential polyadenylation signal with the sequence ATTAAA which is a common natural variant of the conventional sequence AATAAA. The gene was AT-rich, with a GC content of 37.2%. Southern blot analysis of W. bancrofti genomic DNA indicated that the gene is possibly found as a single copy. The actin amino acid sequence of W. bancrofti showed a high degree of homology to the actin of many organisms of different taxonomic groups, but the highest homology was observed with the free-living nematode Plectus acuminatus. This suggests that P. acuminatus may bear a close evolutionary relationship to W. bancrofti.

PubMed: 10704593

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pubmed:10704593

Le document en format XML

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<author><name sortKey="Casinader Saverimuttu, J K" sort="Casinader Saverimuttu, J K" uniqKey="Casinader Saverimuttu J" first="J K" last="Casinader Saverimuttu">J K Casinader Saverimuttu</name>
<affiliation><nlm:affiliation>Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Karunanayake, E H" sort="Karunanayake, E H" uniqKey="Karunanayake E" first="E H" last="Karunanayake">E H Karunanayake</name>
</author>
<author><name sortKey="Chandrasekharan, N V" sort="Chandrasekharan, N V" uniqKey="Chandrasekharan N" first="N V" last="Chandrasekharan">N V Chandrasekharan</name>
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<author><name sortKey="Jayasena, S M" sort="Jayasena, S M" uniqKey="Jayasena S" first="S M" last="Jayasena">S M Jayasena</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular characterisation of the actin gene of the filarial parasite Wuchereria bancrofti.</title>
<author><name sortKey="Casinader Saverimuttu, J K" sort="Casinader Saverimuttu, J K" uniqKey="Casinader Saverimuttu J" first="J K" last="Casinader Saverimuttu">J K Casinader Saverimuttu</name>
<affiliation><nlm:affiliation>Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.</nlm:affiliation>
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<author><name sortKey="Karunanayake, E H" sort="Karunanayake, E H" uniqKey="Karunanayake E" first="E H" last="Karunanayake">E H Karunanayake</name>
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<author><name sortKey="Chandrasekharan, N V" sort="Chandrasekharan, N V" uniqKey="Chandrasekharan N" first="N V" last="Chandrasekharan">N V Chandrasekharan</name>
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<author><name sortKey="Jayasena, S M" sort="Jayasena, S M" uniqKey="Jayasena S" first="S M" last="Jayasena">S M Jayasena</name>
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<series><title level="j">International journal for parasitology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actins (genetics)</term>
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Consensus Sequence</term>
<term>Elephantiasis, Filarial (genetics)</term>
<term>Elephantiasis, Filarial (parasitology)</term>
<term>Gene Library</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Restriction Mapping</term>
<term>Sequence Alignment</term>
<term>TATA Box</term>
<term>Wuchereria bancrofti (genetics)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Actins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Elephantiasis, Filarial</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Consensus Sequence</term>
<term>Gene Library</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Restriction Mapping</term>
<term>Sequence Alignment</term>
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<front><div type="abstract" xml:lang="en">Wuchereria bancrofti is the major cause of lymphatic filariasis in humans. Although it is responsible for this immensely morbid and debilitating disease, very little is known of the basic molecular biology of this parasite, and there is a vast lack of knowledge on its gene organisation. In this study, the actin gene of W. bancrofti has been characterised by sequencing a clone isolated from a genomic DNA library of this parasite. The 5' flanking region had a potential TATA box and a putative mRNA initiation site. The gene had five exons encoding 376 amino acids, and four introns ranging in size from 109 to 190bp. The 3' flanking region had a potential polyadenylation signal with the sequence ATTAAA which is a common natural variant of the conventional sequence AATAAA. The gene was AT-rich, with a GC content of 37.2%. Southern blot analysis of W. bancrofti genomic DNA indicated that the gene is possibly found as a single copy. The actin amino acid sequence of W. bancrofti showed a high degree of homology to the actin of many organisms of different taxonomic groups, but the highest homology was observed with the free-living nematode Plectus acuminatus. This suggests that P. acuminatus may bear a close evolutionary relationship to W. bancrofti.</div>
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<DateCreated><Year>2000</Year>
<Month>06</Month>
<Day>06</Day>
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<DateCompleted><Year>2000</Year>
<Month>06</Month>
<Day>06</Day>
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<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
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<JournalIssue CitedMedium="Print"><Volume>30</Volume>
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<Title>International journal for parasitology</Title>
<ISOAbbreviation>Int. J. Parasitol.</ISOAbbreviation>
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<ArticleTitle>Molecular characterisation of the actin gene of the filarial parasite Wuchereria bancrofti.</ArticleTitle>
<Pagination><MedlinePgn>119-24</MedlinePgn>
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<Abstract><AbstractText>Wuchereria bancrofti is the major cause of lymphatic filariasis in humans. Although it is responsible for this immensely morbid and debilitating disease, very little is known of the basic molecular biology of this parasite, and there is a vast lack of knowledge on its gene organisation. In this study, the actin gene of W. bancrofti has been characterised by sequencing a clone isolated from a genomic DNA library of this parasite. The 5' flanking region had a potential TATA box and a putative mRNA initiation site. The gene had five exons encoding 376 amino acids, and four introns ranging in size from 109 to 190bp. The 3' flanking region had a potential polyadenylation signal with the sequence ATTAAA which is a common natural variant of the conventional sequence AATAAA. The gene was AT-rich, with a GC content of 37.2%. Southern blot analysis of W. bancrofti genomic DNA indicated that the gene is possibly found as a single copy. The actin amino acid sequence of W. bancrofti showed a high degree of homology to the actin of many organisms of different taxonomic groups, but the highest homology was observed with the free-living nematode Plectus acuminatus. This suggests that P. acuminatus may bear a close evolutionary relationship to W. bancrofti.</AbstractText>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
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<MeshHeading><DescriptorName UI="D016385" MajorTopicYN="N">TATA Box</DescriptorName>
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<MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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Molecular characterisation of the actin gene of the filarial parasite Wuchereria bancrofti.

Molecular characterisation of the actin gene of the filarial parasite Wuchereria bancrofti.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki