[Proteic profile and antigenic recognition of extracts from Wuchereria bancrofti L3 infective larvae].
Identifieur interne : 003D66 ( PubMed/Corpus ); précédent : 003D65; suivant : 003D67[Proteic profile and antigenic recognition of extracts from Wuchereria bancrofti L3 infective larvae].
Auteurs : Janaina Miranda ; Amélia Maciel ; Renata Maria Costa Souza ; André Freire Furtado ; Elizabeth MalaguenoSource :
- Revista da Sociedade Brasileira de Medicina Tropical [ 0037-8682 ]
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Animals, Antigens, Helminth (immunology), Blotting, Western, Brazil, Child, Child, Preschool, Chronic Disease, Culex (parasitology), Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Female, Helminth Proteins (analysis), Helminth Proteins (immunology), Humans, Larva (chemistry), Larva (immunology), Male, Middle Aged, Wuchereria bancrofti (chemistry), Wuchereria bancrofti (immunology).
- MESH :
- chemical , analysis : Helminth Proteins.
- chemical , immunology : Antigens, Helminth, Helminth Proteins.
- geographic : Brazil.
- chemistry : Larva, Wuchereria bancrofti.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Larva, Wuchereria bancrofti.
- parasitology : Culex.
- Adolescent, Adult, Aged, Animals, Blotting, Western, Child, Child, Preschool, Chronic Disease, Electrophoresis, Polyacrylamide Gel, Female, Humans, Male, Middle Aged.
Abstract
A study of protein characterization and recognition of the antigenic profile was accomplished in extracts of infective larvae (L3) from Wuchereria bancrofti. Two proteins of relative molecular weight of 49 and 55 kDa were recognized as antigenic in all extracts by the tested sera. The secretory/excretory antigen presented the largest number of recognized bands (105, 100, 76, 55, 49, 39 and 32 kDa) followed by the somatic antigen (100, 76, 55 and 49 kDa) when incubated with pools of sera from healthy individuals resident in endemic areas (normal endemics). Human sera and parasitized blood used to infect mosquitoes in order to obtain L3, were collected from microfilaraemic individuals living in a filariasis endemic area. From 792 persons screened with the thick smear technique, 87 (11%) were positive. No statistical significance was observed between genders. The group between 11 and 19 years of age presented higher percentage of infection (36.8%).
DOI: /S0037-86822005000100006
PubMed: 15717091
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pubmed:15717091Le document en format XML
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Brazil. janamir@cpqam.fiocruz.br</nlm:affiliation></affiliation></author><author><name sortKey="Maciel, Amelia" sort="Maciel, Amelia" uniqKey="Maciel A" first="Amélia" last="Maciel">Amélia Maciel</name></author><author><name sortKey="Souza, Renata Maria Costa" sort="Souza, Renata Maria Costa" uniqKey="Souza R" first="Renata Maria Costa" last="Souza">Renata Maria Costa Souza</name></author><author><name sortKey="Furtado, Andre Freire" sort="Furtado, Andre Freire" uniqKey="Furtado A" first="André Freire" last="Furtado">André Freire Furtado</name></author><author><name sortKey="Malagueno, Elizabeth" sort="Malagueno, Elizabeth" uniqKey="Malagueno E" first="Elizabeth" last="Malagueno">Elizabeth Malagueno</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2005 Jan-Feb</MedlineDate></PubDate></date><idno type="RBID">pubmed:15717091</idno><idno type="pmid">15717091</idno><idno type="doi">/S0037-86822005000100006</idno><idno type="wicri:Area/PubMed/Corpus">003D66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003D66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Proteic profile and antigenic recognition of extracts from Wuchereria bancrofti L3 infective larvae].</title><author><name sortKey="Miranda, Janaina" sort="Miranda, Janaina" uniqKey="Miranda J" first="Janaina" last="Miranda">Janaina Miranda</name><affiliation><nlm:affiliation>Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE. Brazil. janamir@cpqam.fiocruz.br</nlm:affiliation></affiliation></author><author><name sortKey="Maciel, Amelia" sort="Maciel, Amelia" uniqKey="Maciel A" first="Amélia" last="Maciel">Amélia Maciel</name></author><author><name sortKey="Souza, Renata Maria Costa" sort="Souza, Renata Maria Costa" uniqKey="Souza R" first="Renata Maria Costa" last="Souza">Renata Maria Costa Souza</name></author><author><name sortKey="Furtado, Andre Freire" sort="Furtado, Andre Freire" uniqKey="Furtado A" first="André Freire" last="Furtado">André Freire Furtado</name></author><author><name sortKey="Malagueno, Elizabeth" sort="Malagueno, Elizabeth" uniqKey="Malagueno E" first="Elizabeth" last="Malagueno">Elizabeth Malagueno</name></author></analytic><series><title level="j">Revista da Sociedade Brasileira de Medicina Tropical</title><idno type="ISSN">0037-8682</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Animals</term><term>Antigens, Helminth (immunology)</term><term>Blotting, Western</term><term>Brazil</term><term>Child</term><term>Child, Preschool</term><term>Chronic Disease</term><term>Culex (parasitology)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Female</term><term>Helminth Proteins (analysis)</term><term>Helminth Proteins (immunology)</term><term>Humans</term><term>Larva (chemistry)</term><term>Larva (immunology)</term><term>Male</term><term>Middle Aged</term><term>Wuchereria bancrofti (chemistry)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Helminth Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Brazil</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Larva</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Larva</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Culex</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Animals</term><term>Blotting, Western</term><term>Child</term><term>Child, Preschool</term><term>Chronic Disease</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A study of protein characterization and recognition of the antigenic profile was accomplished in extracts of infective larvae (L3) from Wuchereria bancrofti. Two proteins of relative molecular weight of 49 and 55 kDa were recognized as antigenic in all extracts by the tested sera. The secretory/excretory antigen presented the largest number of recognized bands (105, 100, 76, 55, 49, 39 and 32 kDa) followed by the somatic antigen (100, 76, 55 and 49 kDa) when incubated with pools of sera from healthy individuals resident in endemic areas (normal endemics). Human sera and parasitized blood used to infect mosquitoes in order to obtain L3, were collected from microfilaraemic individuals living in a filariasis endemic area. From 792 persons screened with the thick smear technique, 87 (11%) were positive. No statistical significance was observed between genders. The group between 11 and 19 years of age presented higher percentage of infection (36.8%).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15717091</PMID><DateCreated><Year>2005</Year><Month>02</Month><Day>17</Day></DateCreated><DateCompleted><Year>2005</Year><Month>04</Month><Day>14</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0037-8682</ISSN><JournalIssue CitedMedium="Print"><Volume>38</Volume><Issue>1</Issue><PubDate><MedlineDate>2005 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Revista da Sociedade Brasileira de Medicina Tropical</Title><ISOAbbreviation>Rev. Soc. Bras. Med. Trop.</ISOAbbreviation></Journal><ArticleTitle>[Proteic profile and antigenic recognition of extracts from Wuchereria bancrofti L3 infective larvae].</ArticleTitle><Pagination><MedlinePgn>27-32</MedlinePgn></Pagination><Abstract><AbstractText>A study of protein characterization and recognition of the antigenic profile was accomplished in extracts of infective larvae (L3) from Wuchereria bancrofti. Two proteins of relative molecular weight of 49 and 55 kDa were recognized as antigenic in all extracts by the tested sera. The secretory/excretory antigen presented the largest number of recognized bands (105, 100, 76, 55, 49, 39 and 32 kDa) followed by the somatic antigen (100, 76, 55 and 49 kDa) when incubated with pools of sera from healthy individuals resident in endemic areas (normal endemics). Human sera and parasitized blood used to infect mosquitoes in order to obtain L3, were collected from microfilaraemic individuals living in a filariasis endemic area. From 792 persons screened with the thick smear technique, 87 (11%) were positive. No statistical significance was observed between genders. The group between 11 and 19 years of age presented higher percentage of infection (36.8%).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Miranda</LastName><ForeName>Janaina</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE. Brazil. janamir@cpqam.fiocruz.br</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maciel</LastName><ForeName>Amélia</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Souza</LastName><ForeName>Renata Maria Costa</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Furtado</LastName><ForeName>André Freire</ForeName><Initials>AF</Initials></Author><Author ValidYN="Y"><LastName>Malagueno</LastName><ForeName>Elizabeth</ForeName><Initials>E</Initials></Author></AuthorList><Language>por</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><VernacularTitle>Perfil protéico e reconhecimento antigênico de extratos de larvas infectantes (L3) de Wuchereria bancrofti.</VernacularTitle><ArticleDate DateType="Electronic"><Year>2005</Year><Month>01</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Rev Soc Bras Med Trop</MedlineTA><NlmUniqueID>7507456</NlmUniqueID><ISSNLinking>0037-8682</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015801">Helminth Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" 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MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015801" MajorTopicYN="N">Helminth Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>2</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>4</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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