Cloning and expression of a 12 kDa serospecific epitope of Wuchereria bancrofti.
Identifieur interne : 003B97 ( PubMed/Corpus ); précédent : 003B96; suivant : 003B98Cloning and expression of a 12 kDa serospecific epitope of Wuchereria bancrofti.
Auteurs : Pankaj Dabir ; Snehal Dabir ; M V R. ReddySource :
- Immunology and cell biology [ 0818-9641 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (biosynthesis), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Antigens, Helminth (metabolism), Biotin (genetics), Biotin (immunology), Biotin (metabolism), Cloning, Molecular, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Helminth Proteins (genetics), Helminth Proteins (immunology), Helminth Proteins (metabolism), Humans, Immunodominant Epitopes (genetics), Immunodominant Epitopes (immunology), Molecular Sequence Data, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Recombinant Fusion Proteins (metabolism), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology), Wuchereria bancrofti (metabolism).
- MESH :
- chemical , biosynthesis : Antigens, Helminth.
- chemical , genetics : Antigens, Helminth, Biotin, Helminth Proteins, Immunodominant Epitopes, Recombinant Fusion Proteins.
- chemical , immunology : Antigens, Helminth, Biotin, Helminth Proteins, Immunodominant Epitopes, Recombinant Fusion Proteins.
- chemical , metabolism : Antigens, Helminth, Biotin, Helminth Proteins, Recombinant Fusion Proteins.
- blood : Elephantiasis, Filarial.
- diagnosis : Elephantiasis, Filarial.
- genetics : Wuchereria bancrofti.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- metabolism : Wuchereria bancrofti.
- Animals, Cloning, Molecular, Humans, Molecular Sequence Data.
Abstract
The immunoscreening of a microfilarial cDNA library of Wuchereria bancrofti with microfilaraemic sera revealed many positive clones expressing filarial antigens. One immunoreactive clone, designated PMR1, was shown to encode a protein of 114 amino acid residues. The cDNA fragment was subcloned into an expression vector, Pinpoint XaT. The resulting recombinant (r)PMR1-biotin fusion protein was expressed in Escherichia coli (BL21 [DE3] pLys) and was affinity purified on avidin resin. Analysis of sera of different groups for filarial antibodies against rPMR1 showed it to be highly reactive with microfilaraemic and clinical filarial sera compared to its reactivity with endemic and nonendemic controls. This indicates that the gene sequence of cDNA is expressing an immunodominant epitope, which could be useful in serodiagnosis of lymphatic filariasis.
DOI: 10.1111/j.1440-1711.2005.01359.x
PubMed: 16174102
Links to Exploration step
pubmed:16174102Le document en format XML
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<affiliation><nlm:affiliation>Department of Biochemistry, JB Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India.</nlm:affiliation>
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<author><name sortKey="Dabir, Snehal" sort="Dabir, Snehal" uniqKey="Dabir S" first="Snehal" last="Dabir">Snehal Dabir</name>
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<author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name>
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<term>Antigens, Helminth (immunology)</term>
<term>Antigens, Helminth (metabolism)</term>
<term>Biotin (genetics)</term>
<term>Biotin (immunology)</term>
<term>Biotin (metabolism)</term>
<term>Cloning, Molecular</term>
<term>Elephantiasis, Filarial (blood)</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Helminth Proteins (genetics)</term>
<term>Helminth Proteins (immunology)</term>
<term>Helminth Proteins (metabolism)</term>
<term>Humans</term>
<term>Immunodominant Epitopes (genetics)</term>
<term>Immunodominant Epitopes (immunology)</term>
<term>Molecular Sequence Data</term>
<term>Recombinant Fusion Proteins (genetics)</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>Recombinant Fusion Proteins (metabolism)</term>
<term>Wuchereria bancrofti (genetics)</term>
<term>Wuchereria bancrofti (immunology)</term>
<term>Wuchereria bancrofti (metabolism)</term>
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<term>Biotin</term>
<term>Helminth Proteins</term>
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<term>Biotin</term>
<term>Helminth Proteins</term>
<term>Immunodominant Epitopes</term>
<term>Recombinant Fusion Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Helminth</term>
<term>Biotin</term>
<term>Helminth Proteins</term>
<term>Recombinant Fusion Proteins</term>
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<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Wuchereria bancrofti</term>
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<term>Cloning, Molecular</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">The immunoscreening of a microfilarial cDNA library of Wuchereria bancrofti with microfilaraemic sera revealed many positive clones expressing filarial antigens. One immunoreactive clone, designated PMR1, was shown to encode a protein of 114 amino acid residues. The cDNA fragment was subcloned into an expression vector, Pinpoint XaT. The resulting recombinant (r)PMR1-biotin fusion protein was expressed in Escherichia coli (BL21 [DE3] pLys) and was affinity purified on avidin resin. Analysis of sera of different groups for filarial antibodies against rPMR1 showed it to be highly reactive with microfilaraemic and clinical filarial sera compared to its reactivity with endemic and nonendemic controls. This indicates that the gene sequence of cDNA is expressing an immunodominant epitope, which could be useful in serodiagnosis of lymphatic filariasis.</div>
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<ArticleTitle>Cloning and expression of a 12 kDa serospecific epitope of Wuchereria bancrofti.</ArticleTitle>
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<Abstract><AbstractText>The immunoscreening of a microfilarial cDNA library of Wuchereria bancrofti with microfilaraemic sera revealed many positive clones expressing filarial antigens. One immunoreactive clone, designated PMR1, was shown to encode a protein of 114 amino acid residues. The cDNA fragment was subcloned into an expression vector, Pinpoint XaT. The resulting recombinant (r)PMR1-biotin fusion protein was expressed in Escherichia coli (BL21 [DE3] pLys) and was affinity purified on avidin resin. Analysis of sera of different groups for filarial antibodies against rPMR1 showed it to be highly reactive with microfilaraemic and clinical filarial sera compared to its reactivity with endemic and nonendemic controls. This indicates that the gene sequence of cDNA is expressing an immunodominant epitope, which could be useful in serodiagnosis of lymphatic filariasis.</AbstractText>
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