Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
Identifieur interne : 003928 ( PubMed/Corpus ); précédent : 003927; suivant : 003929Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
Auteurs : Daya Ram Pokharel ; Reeta Rai ; Krithika Nandakumar Kodumudi ; Maryada Venkata Rami Reddy ; Sushma RathaurSource :
- Vaccine [ 0264-410X ] ; 2006.
English descriptors
- KwdEn :
- Animals, Brugia malayi (immunology), Buffaloes (parasitology), Collagenases (immunology), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Female, Gerbillinae, Male, Setaria Nematode (enzymology), Setaria Nematode (immunology), Th1 Cells (immunology), Th2 Cells (immunology), Vaccines (immunology), Vaccines (therapeutic use).
- MESH :
- chemical , immunology : Collagenases, Vaccines.
- enzymology : Setaria Nematode.
- immunology : Brugia malayi, Elephantiasis, Filarial, Setaria Nematode, Th1 Cells, Th2 Cells.
- parasitology : Buffaloes.
- prevention & control : Elephantiasis, Filarial.
- chemical , therapeutic use : Vaccines.
- Animals, Female, Gerbillinae, Male.
Abstract
A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava Y, Bhandari YP, Reddy MVR, Harinath BC, Rathaur S. An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.
DOI: 10.1016/j.vaccine.2006.05.103
PubMed: 16870314
Links to Exploration step
pubmed:16870314Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.</title><author><name sortKey="Pokharel, Daya Ram" sort="Pokharel, Daya Ram" uniqKey="Pokharel D" first="Daya Ram" last="Pokharel">Daya Ram Pokharel</name><affiliation><nlm:affiliation>Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, UP, India. drpokharel@rediffmail.com </nlm:affiliation> </affiliation></author><author><name sortKey="Rai, Reeta" sort="Rai, Reeta" uniqKey="Rai R" first="Reeta" last="Rai">Reeta Rai</name></author><author><name sortKey="Nandakumar Kodumudi, Krithika" sort="Nandakumar Kodumudi, Krithika" uniqKey="Nandakumar Kodumudi K" first="Krithika" last="Nandakumar Kodumudi">Krithika Nandakumar Kodumudi</name></author><author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></author><author><name sortKey="Rathaur, Sushma" sort="Rathaur, Sushma" uniqKey="Rathaur S" first="Sushma" last="Rathaur">Sushma Rathaur</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16870314</idno><idno type="pmid">16870314</idno><idno type="doi">10.1016/j.vaccine.2006.05.103</idno><idno type="wicri:Area/PubMed/Corpus">003928</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003928</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.</title><author><name sortKey="Pokharel, Daya Ram" sort="Pokharel, Daya Ram" uniqKey="Pokharel D" first="Daya Ram" last="Pokharel">Daya Ram Pokharel</name><affiliation><nlm:affiliation>Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, UP, India. drpokharel@rediffmail.com </nlm:affiliation> </affiliation></author><author><name sortKey="Rai, Reeta" sort="Rai, Reeta" uniqKey="Rai R" first="Reeta" last="Rai">Reeta Rai</name></author><author><name sortKey="Nandakumar Kodumudi, Krithika" sort="Nandakumar Kodumudi, Krithika" uniqKey="Nandakumar Kodumudi K" first="Krithika" last="Nandakumar Kodumudi">Krithika Nandakumar Kodumudi</name></author><author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></author><author><name sortKey="Rathaur, Sushma" sort="Rathaur, Sushma" uniqKey="Rathaur S" first="Sushma" last="Rathaur">Sushma Rathaur</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brugia malayi (immunology)</term><term>Buffaloes (parasitology)</term><term>Collagenases (immunology)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Female</term><term>Gerbillinae</term><term>Male</term><term>Setaria Nematode (enzymology)</term><term>Setaria Nematode (immunology)</term><term>Th1 Cells (immunology)</term><term>Th2 Cells (immunology)</term><term>Vaccines (immunology)</term><term>Vaccines (therapeutic use)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Collagenases</term><term>Vaccines</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Setaria Nematode</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Setaria Nematode</term><term>Th1 Cells</term><term>Th2 Cells</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Buffaloes</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Vaccines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gerbillinae</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava Y, Bhandari YP, Reddy MVR, Harinath BC, Rathaur S. An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16870314</PMID><DateCreated><Year>2006</Year><Month>08</Month><Day>25</Day></DateCreated><DateCompleted><Year>2006</Year><Month>11</Month><Day>14</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0264-410X</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>37-39</Issue><PubDate><Year>2006</Year><Month>Sep</Month><Day>11</Day></PubDate></JournalIssue><Title>Vaccine</Title><ISOAbbreviation>Vaccine</ISOAbbreviation></Journal><ArticleTitle>Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.</ArticleTitle><Pagination><MedlinePgn>6208-15</MedlinePgn></Pagination><Abstract><AbstractText>A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava Y, Bhandari YP, Reddy MVR, Harinath BC, Rathaur S. An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pokharel</LastName><ForeName>Daya Ram</ForeName><Initials>DR</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi-221005, UP, India. drpokharel@rediffmail.com </Affiliation> </AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rai</LastName><ForeName>Reeta</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Nandakumar Kodumudi</LastName><ForeName>Krithika</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>Maryada Venkata Rami</ForeName><Initials>MV</Initials></Author><Author ValidYN="Y"><LastName>Rathaur</LastName><ForeName>Sushma</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>06</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Vaccine</MedlineTA><NlmUniqueID>8406899</NlmUniqueID><ISSNLinking>0264-410X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014612">Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.24.-</RegistryNumber><NameOfSubstance UI="D017364">Collagenases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002020" MajorTopicYN="N">Buffaloes</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017364" MajorTopicYN="N">Collagenases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005849" MajorTopicYN="N">Gerbillinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017182" MajorTopicYN="N">Setaria Nematode</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018417" MajorTopicYN="N">Th1 Cells</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018418" MajorTopicYN="N">Th2 Cells</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014612" MajorTopicYN="N">Vaccines</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000627" MajorTopicYN="Y">therapeutic use</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>02</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>11</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16870314</ArticleId><ArticleId IdType="pii">S0264-410X(06)00689-X</ArticleId><ArticleId IdType="doi">10.1016/j.vaccine.2006.05.103</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003928 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 003928 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:16870314
|texte= Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:16870314" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |