Persistence of Brugia malayi DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis.
Identifieur interne : 003726 ( PubMed/Corpus ); précédent : 003725; suivant : 003727Persistence of Brugia malayi DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis.
Auteurs : Peter Fischer ; Sara M. Erickson ; Kerstin Fischer ; Jeremy F. Fuchs ; Ramakrishna U. Rao ; Bruce M. Christensen ; Gary J. WeilSource :
- The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 2007.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Helminth.
- diagnosis : Elephantiasis, Filarial.
- genetics : Brugia malayi.
- isolation & purification : Brugia malayi.
- methods : Polymerase Chain Reaction.
- parasitology : Aedes, Culex.
- transmission : Elephantiasis, Filarial.
- Animals, Humans, Sensitivity and Specificity.
Abstract
Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae, especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area.
PubMed: 17360875
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pubmed:17360875Le document en format XML
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Erickson</name></author><author><name sortKey="Fischer, Kerstin" sort="Fischer, Kerstin" uniqKey="Fischer K" first="Kerstin" last="Fischer">Kerstin Fischer</name></author><author><name sortKey="Fuchs, Jeremy F" sort="Fuchs, Jeremy F" uniqKey="Fuchs J" first="Jeremy F" last="Fuchs">Jeremy F. Fuchs</name></author><author><name sortKey="Rao, Ramakrishna U" sort="Rao, Ramakrishna U" uniqKey="Rao R" first="Ramakrishna U" last="Rao">Ramakrishna U. Rao</name></author><author><name sortKey="Christensen, Bruce M" sort="Christensen, Bruce M" uniqKey="Christensen B" first="Bruce M" last="Christensen">Bruce M. Christensen</name></author><author><name sortKey="Weil, Gary J" sort="Weil, Gary J" uniqKey="Weil G" first="Gary J" last="Weil">Gary J. Weil</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17360875</idno><idno type="pmid">17360875</idno><idno type="wicri:Area/PubMed/Corpus">003726</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003726</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Persistence of Brugia malayi DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis.</title><author><name sortKey="Fischer, Peter" sort="Fischer, Peter" uniqKey="Fischer P" first="Peter" last="Fischer">Peter Fischer</name><affiliation><nlm:affiliation>Department of Internal Medicine, Infectious Diseases Division, Washington University School of Medicine, St. Louis, Missouri 63110, USA. pufische@im.wustl.edu</nlm:affiliation></affiliation></author><author><name sortKey="Erickson, Sara M" sort="Erickson, Sara M" uniqKey="Erickson S" first="Sara M" last="Erickson">Sara M. Erickson</name></author><author><name sortKey="Fischer, Kerstin" sort="Fischer, Kerstin" uniqKey="Fischer K" first="Kerstin" last="Fischer">Kerstin Fischer</name></author><author><name sortKey="Fuchs, Jeremy F" sort="Fuchs, Jeremy F" uniqKey="Fuchs J" first="Jeremy F" last="Fuchs">Jeremy F. Fuchs</name></author><author><name sortKey="Rao, Ramakrishna U" sort="Rao, Ramakrishna U" uniqKey="Rao R" first="Ramakrishna U" last="Rao">Ramakrishna U. Rao</name></author><author><name sortKey="Christensen, Bruce M" sort="Christensen, Bruce M" uniqKey="Christensen B" first="Bruce M" last="Christensen">Bruce M. Christensen</name></author><author><name sortKey="Weil, Gary J" sort="Weil, Gary J" uniqKey="Weil G" first="Gary J" last="Weil">Gary J. Weil</name></author></analytic><series><title level="j">The American journal of tropical medicine and hygiene</title><idno type="ISSN">0002-9637</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aedes (parasitology)</term><term>Animals</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (isolation & purification)</term><term>Culex (parasitology)</term><term>DNA, Helminth (analysis)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (transmission)</term><term>Humans</term><term>Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Aedes</term><term>Culex</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae, especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17360875</PMID><DateCreated><Year>2007</Year><Month>03</Month><Day>15</Day></DateCreated><DateCompleted><Year>2007</Year><Month>04</Month><Day>17</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0002-9637</ISSN><JournalIssue CitedMedium="Print"><Volume>76</Volume><Issue>3</Issue><PubDate><Year>2007</Year><Month>Mar</Month></PubDate></JournalIssue><Title>The American journal of tropical medicine and hygiene</Title><ISOAbbreviation>Am. J. Trop. Med. Hyg.</ISOAbbreviation></Journal><ArticleTitle>Persistence of Brugia malayi DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis.</ArticleTitle><Pagination><MedlinePgn>502-7</MedlinePgn></Pagination><Abstract><AbstractText>Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae, especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fischer</LastName><ForeName>Peter</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Internal Medicine, Infectious Diseases Division, Washington University School of Medicine, St. Louis, Missouri 63110, USA. pufische@im.wustl.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Erickson</LastName><ForeName>Sara M</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Fischer</LastName><ForeName>Kerstin</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Fuchs</LastName><ForeName>Jeremy F</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Rao</LastName><ForeName>Ramakrishna U</ForeName><Initials>RU</Initials></Author><Author ValidYN="Y"><LastName>Christensen</LastName><ForeName>Bruce M</ForeName><Initials>BM</Initials></Author><Author ValidYN="Y"><LastName>Weil</LastName><ForeName>Gary J</ForeName><Initials>GJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>U01 AI035855</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R37 AI019769</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U19 AI065715-02</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 AI019769-21</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 AI019769</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U01 AI035855-10S1</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U19 AI065715-01</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32 AI007414</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>AI-35855</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>AI-19769</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Am J Trop Med Hyg</MedlineTA><NlmUniqueID>0370507</NlmUniqueID><ISSNLinking>0002-9637</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018509">DNA, Helminth</NameOfSubstance></Chemical></ChemicalList><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 2006 May;74(5):779-85</RefSource><PMID Version="1">16687680</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mem Inst Oswaldo Cruz. 1999 Sep-Oct;94(5):591-6</RefSource><PMID Version="1">10464399</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 2006 Jun;74(6):1026-33</RefSource><PMID Version="1">16760515</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Filaria J. 2006 Feb 24;5:2</RefSource><PMID Version="1">16504131</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann Trop Med Parasitol. 2002 Dec;96(8):809-21</RefSource><PMID Version="1">12625936</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 2000 Mar;62(3):363-7</RefSource><PMID Version="1">11037778</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Adv Parasitol. 2006;61:395-441</RefSource><PMID Version="1">16735170</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 2003 Mar;68(3):286-93</RefSource><PMID Version="1">12685631</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biochem Parasitol. 2003 Aug 11;130(1):43-50</RefSource><PMID Version="1">14550895</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann Trop Med Parasitol. 2002 Dec;96 Suppl 2:S41-6</RefSource><PMID Version="1">12625916</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 2005 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Version="1">16564361</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Filaria J. 2003 Jul 7;2(1):11</RefSource><PMID Version="1">12890288</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Invertebr Pathol. 1984 Nov;44(3):267-74</RefSource><PMID Version="1">6501919</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000330" MajorTopicYN="N">Aedes</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003465" MajorTopicYN="N">Culex</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018509" MajorTopicYN="N">DNA, Helminth</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000635" MajorTopicYN="Y">transmission</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList><OtherID 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