Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model.
Identifieur interne : 003689 ( PubMed/Corpus ); précédent : 003688; suivant : 003690Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model.
Auteurs : Sivasakthivel Thirugnanam ; Pandurangan Pandiaraja ; Kalyanasundaram Ramaswamy ; Vadivel Murugan ; Munirathinam Gnanasekar ; Krithika Nandakumar ; Maryada Venkata Rami Reddy ; Perumal KalirajSource :
- Experimental parasitology [ 0014-4894 ] ; 2007.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (biosynthesis), Antibodies, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Brugia malayi (immunology), Cytokines (biosynthesis), Cytokines (genetics), DNA, Helminth (immunology), Diffusion Chambers, Culture, Disease Models, Animal, Elephantiasis, Filarial (prevention & control), Gerbillinae, Helminth Proteins (genetics), Helminth Proteins (immunology), Immunity, Cellular, Immunization (methods), Lymphocyte Activation, Male, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Th2 Cells (immunology), Vaccines (immunology), Vaccines, DNA (immunology), Vaccines, Synthetic (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Helminth, Cytokines.
- chemical , blood : Antibodies, Helminth.
- chemical , genetics : Antigens, Helminth, Cytokines, Helminth Proteins, Recombinant Proteins.
- chemical , immunology : Antigens, Helminth, DNA, Helminth, Helminth Proteins, Recombinant Proteins, Vaccines, Vaccines, DNA, Vaccines, Synthetic.
- immunology : Brugia malayi, Th2 Cells.
- methods : Immunization.
- prevention & control : Elephantiasis, Filarial.
- Animals, Diffusion Chambers, Culture, Disease Models, Animal, Gerbillinae, Immunity, Cellular, Lymphocyte Activation, Male.
Abstract
Immunization of jirds with Bm-alt-2 elicited partial protection against challenge infection with the filarial parasite Brugia malayi. In this study, we initially compared the protective immune responses elicited following immunization with recombinant Bm-ALT-2 protein regimen and Bm-alt-2 DNA regimen. These studies showed that protein vaccination conferred approximately 75% protection compared to DNA vaccination that conferred only 57% protection. Analysis of the protective immune responses showed that the protein immunization promoted a Th2-biased response with an increase in IL-4, IL-5 and IgG1 responses, whereas, the DNA vaccine promoted a Th1-biased response with profound IFN-gamma and IgG2a responses. Since protein vaccination gave better results than DNA vaccination, we then wanted to evaluate whether a prime-boost vaccination that combined DNA prime and protein boost will significantly increase the protective responses induced by the protein vaccine. Our results suggest that prime-boost vaccination had no added advantage and was comparatively less effective (64% protection) than the Bm-ALT-2 protein alone vaccination. Prime boost vaccination generated mixed Th1/Th2 responses with a slightly diminished Th2 responses compared to protein vaccination. Thus, our results suggest that Bm-ALT-2 protein vaccination regimen may be slightly better than prime-boost vaccine regimen and the mechanism of protection appears to be largely mediated by a Th2-biased response.
DOI: 10.1016/j.exppara.2007.02.017
PubMed: 17442307
Links to Exploration step
pubmed:17442307Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model.</title><author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name><affiliation><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai 600 025, India.</nlm:affiliation></affiliation></author><author><name sortKey="Pandiaraja, Pandurangan" sort="Pandiaraja, Pandurangan" uniqKey="Pandiaraja P" first="Pandurangan" last="Pandiaraja">Pandurangan Pandiaraja</name></author><author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name></author><author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></author><author><name sortKey="Gnanasekar, Munirathinam" sort="Gnanasekar, Munirathinam" uniqKey="Gnanasekar M" first="Munirathinam" last="Gnanasekar">Munirathinam Gnanasekar</name></author><author><name sortKey="Nandakumar, Krithika" sort="Nandakumar, Krithika" uniqKey="Nandakumar K" first="Krithika" last="Nandakumar">Krithika Nandakumar</name></author><author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17442307</idno><idno type="pmid">17442307</idno><idno type="doi">10.1016/j.exppara.2007.02.017</idno><idno type="wicri:Area/PubMed/Corpus">003689</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003689</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model.</title><author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name><affiliation><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai 600 025, India.</nlm:affiliation></affiliation></author><author><name sortKey="Pandiaraja, Pandurangan" sort="Pandiaraja, Pandurangan" uniqKey="Pandiaraja P" first="Pandurangan" last="Pandiaraja">Pandurangan Pandiaraja</name></author><author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name></author><author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></author><author><name sortKey="Gnanasekar, Munirathinam" sort="Gnanasekar, Munirathinam" uniqKey="Gnanasekar M" first="Munirathinam" last="Gnanasekar">Munirathinam Gnanasekar</name></author><author><name sortKey="Nandakumar, Krithika" sort="Nandakumar, Krithika" uniqKey="Nandakumar K" first="Krithika" last="Nandakumar">Krithika Nandakumar</name></author><author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></analytic><series><title level="j">Experimental parasitology</title><idno type="ISSN">0014-4894</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (biosynthesis)</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (immunology)</term><term>Cytokines (biosynthesis)</term><term>Cytokines (genetics)</term><term>DNA, Helminth (immunology)</term><term>Diffusion Chambers, Culture</term><term>Disease Models, Animal</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Gerbillinae</term><term>Helminth Proteins (genetics)</term><term>Helminth Proteins (immunology)</term><term>Immunity, Cellular</term><term>Immunization (methods)</term><term>Lymphocyte Activation</term><term>Male</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Th2 Cells (immunology)</term><term>Vaccines (immunology)</term><term>Vaccines, DNA (immunology)</term><term>Vaccines, Synthetic (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Helminth</term><term>Cytokines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Cytokines</term><term>Helminth Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term><term>DNA, Helminth</term><term>Helminth Proteins</term><term>Recombinant Proteins</term><term>Vaccines</term><term>Vaccines, DNA</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Th2 Cells</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Diffusion Chambers, Culture</term><term>Disease Models, Animal</term><term>Gerbillinae</term><term>Immunity, Cellular</term><term>Lymphocyte Activation</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Immunization of jirds with Bm-alt-2 elicited partial protection against challenge infection with the filarial parasite Brugia malayi. In this study, we initially compared the protective immune responses elicited following immunization with recombinant Bm-ALT-2 protein regimen and Bm-alt-2 DNA regimen. These studies showed that protein vaccination conferred approximately 75% protection compared to DNA vaccination that conferred only 57% protection. Analysis of the protective immune responses showed that the protein immunization promoted a Th2-biased response with an increase in IL-4, IL-5 and IgG1 responses, whereas, the DNA vaccine promoted a Th1-biased response with profound IFN-gamma and IgG2a responses. Since protein vaccination gave better results than DNA vaccination, we then wanted to evaluate whether a prime-boost vaccination that combined DNA prime and protein boost will significantly increase the protective responses induced by the protein vaccine. Our results suggest that prime-boost vaccination had no added advantage and was comparatively less effective (64% protection) than the Bm-ALT-2 protein alone vaccination. Prime boost vaccination generated mixed Th1/Th2 responses with a slightly diminished Th2 responses compared to protein vaccination. Thus, our results suggest that Bm-ALT-2 protein vaccination regimen may be slightly better than prime-boost vaccine regimen and the mechanism of protection appears to be largely mediated by a Th2-biased response.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17442307</PMID><DateCreated><Year>2007</Year><Month>06</Month><Day>11</Day></DateCreated><DateCompleted><Year>2007</Year><Month>08</Month><Day>01</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0014-4894</ISSN><JournalIssue CitedMedium="Print"><Volume>116</Volume><Issue>4</Issue><PubDate><Year>2007</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Experimental parasitology</Title><ISOAbbreviation>Exp. Parasitol.</ISOAbbreviation></Journal><ArticleTitle>Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model.</ArticleTitle><Pagination><MedlinePgn>483-91</MedlinePgn></Pagination><Abstract><AbstractText>Immunization of jirds with Bm-alt-2 elicited partial protection against challenge infection with the filarial parasite Brugia malayi. In this study, we initially compared the protective immune responses elicited following immunization with recombinant Bm-ALT-2 protein regimen and Bm-alt-2 DNA regimen. These studies showed that protein vaccination conferred approximately 75% protection compared to DNA vaccination that conferred only 57% protection. Analysis of the protective immune responses showed that the protein immunization promoted a Th2-biased response with an increase in IL-4, IL-5 and IgG1 responses, whereas, the DNA vaccine promoted a Th1-biased response with profound IFN-gamma and IgG2a responses. Since protein vaccination gave better results than DNA vaccination, we then wanted to evaluate whether a prime-boost vaccination that combined DNA prime and protein boost will significantly increase the protective responses induced by the protein vaccine. Our results suggest that prime-boost vaccination had no added advantage and was comparatively less effective (64% protection) than the Bm-ALT-2 protein alone vaccination. Prime boost vaccination generated mixed Th1/Th2 responses with a slightly diminished Th2 responses compared to protein vaccination. Thus, our results suggest that Bm-ALT-2 protein vaccination regimen may be slightly better than prime-boost vaccine regimen and the mechanism of protection appears to be largely mediated by a Th2-biased response.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Thirugnanam</LastName><ForeName>Sivasakthivel</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre for Biotechnology, Anna University, Chennai 600 025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pandiaraja</LastName><ForeName>Pandurangan</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Ramaswamy</LastName><ForeName>Kalyanasundaram</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Murugan</LastName><ForeName>Vadivel</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Gnanasekar</LastName><ForeName>Munirathinam</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Nandakumar</LastName><ForeName>Krithika</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>Maryada Venkata Rami</ForeName><Initials>MV</Initials></Author><Author ValidYN="Y"><LastName>Kaliraj</LastName><ForeName>Perumal</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 AI064745</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 AI064745-02</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R56 AI064745</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R56 AI064745-01A1</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>03</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Exp Parasitol</MedlineTA><NlmUniqueID>0370713</NlmUniqueID><ISSNLinking>0014-4894</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C521555">ALT-2 protein, Brugia malayi</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018509">DNA, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015801">Helminth Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014612">Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014614">Vaccines, Synthetic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Infect Dis. 2001 Jan 15;183(2):303-312</RefSource><PMID Version="1">11110648</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2000 Jul;68(7):4174-9</RefSource><PMID Version="1">10858234</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biochem Parasitol. 2001 Nov;118(1):89-96</RefSource><PMID Version="1">11704277</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Med Microbiol Immunol. 2003 Feb;192(1):23-31</RefSource><PMID Version="1">12592560</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Ann Trop Med Parasitol. 2002 Dec;96 Suppl 2:S97-104</RefSource><PMID Version="1">12625923</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vet Microbiol. 2003 Jun 10;93(4):349-59</RefSource><PMID Version="1">12713896</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Trends Parasitol. 2003 Nov;19(11):516-22</RefSource><PMID Version="1">14580963</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2004 Aug;72(8):4707-15</RefSource><PMID Version="1">15271932</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11088-93</RefSource><PMID Version="1">15258286</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 2004 Sep 9;22(27-28):3622-7</RefSource><PMID Version="1">15315841</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Jpn J Exp Med. 1979 Apr;49(2):117-21</RefSource><PMID Version="1">384057</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 1989 Jun;40(6):598-604</RefSource><PMID Version="1">2662785</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Immunol. 1992 Jun 15;148(12):3733-9</RefSource><PMID Version="1">1351086</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Immunol. 1993 Mar 1;150(5):1881-5</RefSource><PMID Version="1">8436822</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 1993 Dec;49(6):783-8</RefSource><PMID Version="1">8279644</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Immunol. 1995 May;25(5):1477-80</RefSource><PMID Version="1">7774653</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell Immunol. 1996 Nov 1;173(2):192-7</RefSource><PMID Version="1">8912876</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Pharm Sci. 1996 Dec;85(12):1294-300</RefSource><PMID Version="1">8961142</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 1997 Jun;15(8):842-5</RefSource><PMID Version="1">9234529</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9378-83</RefSource><PMID Version="1">9256490</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Immunol Cell Biol. 1997 Aug;75(4):370-5</RefSource><PMID Version="1">9315480</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Hum Gene Ther. 1998 Sep 1;9(13):1899-907</RefSource><PMID Version="1">9741428</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Bull World Health Organ. 1999;77(2):138-43</RefSource><PMID Version="1">10083712</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Exp Parasitol. 1999 Apr;91(4):334-40</RefSource><PMID Version="1">10092477</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 1999 Aug 20;18(1-2):76-81</RefSource><PMID Version="1">10501237</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2004 Dec;72(12):6945-50</RefSource><PMID Version="1">15557616</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Microbiol Immunol. 2004;48(12):945-55</RefSource><PMID Version="1">15611611</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 2005 May 16;23(26):3446-52</RefSource><PMID Version="1">15837368</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Am J Trop Med Hyg. 2005 Oct;73(4):753-8</RefSource><PMID Version="1">16222021</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Lancet. 2006 Mar 25;367(9515):992-9</RefSource><PMID Version="1">16564361</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 2006 Jul 26;24(31-32):5787-99</RefSource><PMID Version="1">16759763</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 1999 Nov 12;18(7-8):647-55</RefSource><PMID Version="1">10547424</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Immunol. 1999 Dec 15;163(12):6448-54</RefSource><PMID Version="1">10586035</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 2000 Jan 31;18(14):1367-73</RefSource><PMID Version="1">10618534</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2000 May;68(5):2513-7</RefSource><PMID Version="1">10768938</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2000 May;68(5):3034-5</RefSource><PMID Version="1">10769010</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Parasite Immunol. 2000 May;22(5):249-57</RefSource><PMID Version="1">10792764</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2000 Jun;68(6):3651-6</RefSource><PMID Version="1">10816524</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Gut. 2001 Jun;48(6):765-73</RefSource><PMID Version="1">11358893</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018509" MajorTopicYN="N">DNA, Helminth</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015194" MajorTopicYN="N">Diffusion Chambers, Culture</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005849" MajorTopicYN="N">Gerbillinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015801" MajorTopicYN="N">Helminth Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007111" MajorTopicYN="N">Immunity, Cellular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007114" MajorTopicYN="N">Immunization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008213" MajorTopicYN="N">Lymphocyte Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018418" MajorTopicYN="N">Th2 Cells</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014612" MajorTopicYN="N">Vaccines</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014614" MajorTopicYN="N">Vaccines, Synthetic</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">NIHMS126827</OtherID><OtherID Source="NLM">PMC2763209</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>09</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2007</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>4</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>8</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>4</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17442307</ArticleId><ArticleId IdType="pii">S0014-4894(07)00061-6</ArticleId><ArticleId IdType="doi">10.1016/j.exppara.2007.02.017</ArticleId><ArticleId IdType="pmc">PMC2763209</ArticleId><ArticleId IdType="mid">NIHMS126827</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003689 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 003689 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:17442307
|texte= Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:17442307" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |