Intraspecies variation of Brugia spp. in cat reservoirs using complete ITS sequences.
Identifieur interne : 002F80 ( PubMed/Corpus ); précédent : 002F79; suivant : 002F81Intraspecies variation of Brugia spp. in cat reservoirs using complete ITS sequences.
Auteurs : Supatra Areekit ; Pirom Singhaphan ; Sintawee Khuchareontaworn ; Pornpimon Kanjanavas ; Thayat Sriyaphai ; Arda Pakpitchareon ; Paisan Khawsak ; Kosum ChansiriSource :
- Parasitology research [ 1432-1955 ] ; 2009.
English descriptors
- KwdEn :
- Animals, Biodiversity, Blood (parasitology), Brugia malayi (classification), Brugia malayi (genetics), Brugia malayi (isolation & purification), Brugia pahangi (classification), Brugia pahangi (genetics), Brugia pahangi (isolation & purification), Cat Diseases (parasitology), Cats, DNA, Helminth (chemistry), DNA, Helminth (genetics), DNA, Ribosomal Spacer (chemistry), DNA, Ribosomal Spacer (genetics), Disease Reservoirs, Elephantiasis, Filarial (parasitology), Elephantiasis, Filarial (veterinary), Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Thailand.
- MESH :
- chemical , chemistry : DNA, Helminth, DNA, Ribosomal Spacer.
- geographic : Thailand.
- classification : Brugia malayi, Brugia pahangi.
- genetics : Brugia malayi, Brugia pahangi, DNA, Helminth, DNA, Ribosomal Spacer.
- isolation & purification : Brugia malayi, Brugia pahangi.
- parasitology : Blood, Cat Diseases, Elephantiasis, Filarial.
- veterinary : Elephantiasis, Filarial.
- Animals, Biodiversity, Cats, Disease Reservoirs, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA.
Abstract
The internal transcribed spacer (ITS) region was used to study the intraspecies variation of Brugia spp. in cat reservoirs. Blood specimens from seven naturally infected cats were collected from two different geographical brugian-endemic areas in Thailand. The DNAPAR tree of these Brugia spp. was constructed using a maximum likelihood approach based on ITS nucleotide sequences and was compared to those of Brugia malayi, Brugia pahangi, and Dirofilaria immitis that were previously reported in GenBank. The phylogenetic trees inferred from ITS1, ITS2, and complete ITS sequences indicated that B. malayi and B. pahangi were separated into two clades, and subgroups were generated within each clade. The data revealed that ITS2 sequences were less informative than ITS1 for studying intraspecies variation of Brugia spp. Our results are primary data for intraspecies variation of B. malayi and B. pahangi in cat reservoirs. The information could be applicable for studying the molecular epidemiology and the dynamic nature of the parasites.
DOI: 10.1007/s00436-009-1352-x
PubMed: 19205740
Links to Exploration step
pubmed:19205740Le document en format XML
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sortKey="Sriyaphai, Thayat" sort="Sriyaphai, Thayat" uniqKey="Sriyaphai T" first="Thayat" last="Sriyaphai">Thayat Sriyaphai</name></author><author><name sortKey="Pakpitchareon, Arda" sort="Pakpitchareon, Arda" uniqKey="Pakpitchareon A" first="Arda" last="Pakpitchareon">Arda Pakpitchareon</name></author><author><name sortKey="Khawsak, Paisan" sort="Khawsak, Paisan" uniqKey="Khawsak P" first="Paisan" last="Khawsak">Paisan Khawsak</name></author><author><name sortKey="Chansiri, Kosum" sort="Chansiri, Kosum" uniqKey="Chansiri K" first="Kosum" last="Chansiri">Kosum Chansiri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19205740</idno><idno type="pmid">19205740</idno><idno type="doi">10.1007/s00436-009-1352-x</idno><idno type="wicri:Area/PubMed/Corpus">002F80</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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Kanjanavas</name></author><author><name sortKey="Sriyaphai, Thayat" sort="Sriyaphai, Thayat" uniqKey="Sriyaphai T" first="Thayat" last="Sriyaphai">Thayat Sriyaphai</name></author><author><name sortKey="Pakpitchareon, Arda" sort="Pakpitchareon, Arda" uniqKey="Pakpitchareon A" first="Arda" last="Pakpitchareon">Arda Pakpitchareon</name></author><author><name sortKey="Khawsak, Paisan" sort="Khawsak, Paisan" uniqKey="Khawsak P" first="Paisan" last="Khawsak">Paisan Khawsak</name></author><author><name sortKey="Chansiri, Kosum" sort="Chansiri, Kosum" uniqKey="Chansiri K" first="Kosum" last="Chansiri">Kosum Chansiri</name></author></analytic><series><title level="j">Parasitology research</title><idno type="eISSN">1432-1955</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biodiversity</term><term>Blood (parasitology)</term><term>Brugia malayi (classification)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (isolation & purification)</term><term>Brugia pahangi (classification)</term><term>Brugia pahangi (genetics)</term><term>Brugia pahangi (isolation & purification)</term><term>Cat Diseases (parasitology)</term><term>Cats</term><term>DNA, Helminth (chemistry)</term><term>DNA, Helminth (genetics)</term><term>DNA, Ribosomal Spacer (chemistry)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Disease Reservoirs</term><term>Elephantiasis, Filarial (parasitology)</term><term>Elephantiasis, Filarial (veterinary)</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Thailand</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Helminth</term><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Thailand</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Brugia malayi</term><term>Brugia pahangi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Brugia pahangi</term><term>DNA, Helminth</term><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia malayi</term><term>Brugia pahangi</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Blood</term><term>Cat Diseases</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biodiversity</term><term>Cats</term><term>Disease Reservoirs</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The internal transcribed spacer (ITS) region was used to study the intraspecies variation of Brugia spp. in cat reservoirs. Blood specimens from seven naturally infected cats were collected from two different geographical brugian-endemic areas in Thailand. The DNAPAR tree of these Brugia spp. was constructed using a maximum likelihood approach based on ITS nucleotide sequences and was compared to those of Brugia malayi, Brugia pahangi, and Dirofilaria immitis that were previously reported in GenBank. The phylogenetic trees inferred from ITS1, ITS2, and complete ITS sequences indicated that B. malayi and B. pahangi were separated into two clades, and subgroups were generated within each clade. The data revealed that ITS2 sequences were less informative than ITS1 for studying intraspecies variation of Brugia spp. Our results are primary data for intraspecies variation of B. malayi and B. pahangi in cat reservoirs. The information could be applicable for studying the molecular epidemiology and the dynamic nature of the parasites.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19205740</PMID><DateCreated><Year>2009</Year><Month>04</Month><Day>23</Day></DateCreated><DateCompleted><Year>2009</Year><Month>07</Month><Day>06</Day></DateCompleted><DateRevised><Year>2009</Year><Month>04</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1955</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>6</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Parasitology research</Title><ISOAbbreviation>Parasitol. Res.</ISOAbbreviation></Journal><ArticleTitle>Intraspecies variation of Brugia spp. in cat reservoirs using complete ITS sequences.</ArticleTitle><Pagination><MedlinePgn>1465-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00436-009-1352-x</ELocationID><Abstract><AbstractText>The internal transcribed spacer (ITS) region was used to study the intraspecies variation of Brugia spp. in cat reservoirs. Blood specimens from seven naturally infected cats were collected from two different geographical brugian-endemic areas in Thailand. The DNAPAR tree of these Brugia spp. was constructed using a maximum likelihood approach based on ITS nucleotide sequences and was compared to those of Brugia malayi, Brugia pahangi, and Dirofilaria immitis that were previously reported in GenBank. The phylogenetic trees inferred from ITS1, ITS2, and complete ITS sequences indicated that B. malayi and B. pahangi were separated into two clades, and subgroups were generated within each clade. The data revealed that ITS2 sequences were less informative than ITS1 for studying intraspecies variation of Brugia spp. Our results are primary data for intraspecies variation of B. malayi and B. pahangi in cat reservoirs. The information could be applicable for studying the molecular epidemiology and the dynamic nature of the parasites.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Areekit</LastName><ForeName>Supatra</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singhaphan</LastName><ForeName>Pirom</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Khuchareontaworn</LastName><ForeName>Sintawee</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kanjanavas</LastName><ForeName>Pornpimon</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Sriyaphai</LastName><ForeName>Thayat</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Pakpitchareon</LastName><ForeName>Arda</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Khawsak</LastName><ForeName>Paisan</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Chansiri</LastName><ForeName>Kosum</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>02</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Parasitol Res</MedlineTA><NlmUniqueID>8703571</NlmUniqueID><ISSNLinking>0932-0113</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018509">DNA, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="Y">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001769" 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Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013785" MajorTopicYN="N" Type="Geographic">Thailand</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>04</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>2</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>2</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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