Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.
Identifieur interne : 002B73 ( PubMed/Corpus ); précédent : 002B72; suivant : 002B74Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.
Auteurs : Moorthy Sakthidevi ; Vadivel Murugan ; Sugeerappa Laxmanappa Hoti ; Perumal KalirajSource :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2010.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (genetics), Brugia malayi (genetics), DNA, Helminth (genetics), Diagnosis, Differential, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Genetic Markers (genetics), Introns (genetics), Models, Genetic, Polymerase Chain Reaction, Recombinant Proteins (genetics), Species Specificity, Tandem Repeat Sequences, Wuchereria bancrofti (genetics).
- MESH :
- chemical , genetics : Antigens, Helminth, DNA, Helminth, Genetic Markers, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- genetics : Brugia malayi, Introns, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Diagnosis, Differential, Models, Genetic, Polymerase Chain Reaction, Species Specificity, Tandem Repeat Sequences.
Abstract
Polymerase chain reaction based methods are promising tools for the monitoring and evaluation of the Global Program for the Elimination of Lymphatic Filariasis. The currently available PCR methods do not differentiate the DNA of Wuchereria bancrofti or Brugia malayi by a single PCR and hence are cumbersome. Therefore, we designed a single step PCR strategy for differentiating Bancroftian infection from Brugian infection based on a newly identified gene from the W. bancrofti genome, abundant larval transcript-2 (alt-2), which is abundantly expressed. The difference in PCR product sizes generated from the presence or absence of evolutionarily altered tandem repeats in alt-2 intron-3 differentiated W. bancrofti from B. malayi. The analysis was performed on the genomic DNA of microfilariae from a number of patient blood samples or microfilariae positive slides from different Indian geographical regions. The assay gave consistent results, differentiating the two filarial parasite species accurately. This alt-2 intron-3 based PCR assay can be a potential tool for the diagnosis and differentiation of co-infections by lymphatic filarial parasites.
DOI: 10.1016/j.meegid.2010.01.012
PubMed: 20132908
Links to Exploration step
pubmed:20132908Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.</title><author><name sortKey="Sakthidevi, Moorthy" sort="Sakthidevi, Moorthy" uniqKey="Sakthidevi M" first="Moorthy" last="Sakthidevi">Moorthy Sakthidevi</name><affiliation><nlm:affiliation>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025, India.</nlm:affiliation></affiliation></author><author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></author><author><name sortKey="Hoti, Sugeerappa Laxmanappa" sort="Hoti, Sugeerappa Laxmanappa" uniqKey="Hoti S" first="Sugeerappa Laxmanappa" last="Hoti">Sugeerappa Laxmanappa Hoti</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20132908</idno><idno type="pmid">20132908</idno><idno type="doi">10.1016/j.meegid.2010.01.012</idno><idno type="wicri:Area/PubMed/Corpus">002B73</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B73</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.</title><author><name sortKey="Sakthidevi, Moorthy" sort="Sakthidevi, Moorthy" uniqKey="Sakthidevi M" first="Moorthy" last="Sakthidevi">Moorthy Sakthidevi</name><affiliation><nlm:affiliation>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025, India.</nlm:affiliation></affiliation></author><author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name></author><author><name sortKey="Hoti, Sugeerappa Laxmanappa" sort="Hoti, Sugeerappa Laxmanappa" uniqKey="Hoti S" first="Sugeerappa Laxmanappa" last="Hoti">Sugeerappa Laxmanappa Hoti</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Helminth (genetics)</term><term>Brugia malayi (genetics)</term><term>DNA, Helminth (genetics)</term><term>Diagnosis, Differential</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Genetic Markers (genetics)</term><term>Introns (genetics)</term><term>Models, Genetic</term><term>Polymerase Chain Reaction</term><term>Recombinant Proteins (genetics)</term><term>Species Specificity</term><term>Tandem Repeat Sequences</term><term>Wuchereria bancrofti (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>DNA, Helminth</term><term>Genetic Markers</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Introns</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Diagnosis, Differential</term><term>Models, Genetic</term><term>Polymerase Chain Reaction</term><term>Species Specificity</term><term>Tandem Repeat Sequences</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polymerase chain reaction based methods are promising tools for the monitoring and evaluation of the Global Program for the Elimination of Lymphatic Filariasis. The currently available PCR methods do not differentiate the DNA of Wuchereria bancrofti or Brugia malayi by a single PCR and hence are cumbersome. Therefore, we designed a single step PCR strategy for differentiating Bancroftian infection from Brugian infection based on a newly identified gene from the W. bancrofti genome, abundant larval transcript-2 (alt-2), which is abundantly expressed. The difference in PCR product sizes generated from the presence or absence of evolutionarily altered tandem repeats in alt-2 intron-3 differentiated W. bancrofti from B. malayi. The analysis was performed on the genomic DNA of microfilariae from a number of patient blood samples or microfilariae positive slides from different Indian geographical regions. The assay gave consistent results, differentiating the two filarial parasite species accurately. This alt-2 intron-3 based PCR assay can be a potential tool for the diagnosis and differentiation of co-infections by lymphatic filarial parasites.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20132908</PMID><DateCreated><Year>2010</Year><Month>04</Month><Day>21</Day></DateCreated><DateCompleted><Year>2010</Year><Month>07</Month><Day>27</Day></DateCompleted><DateRevised><Year>2010</Year><Month>04</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1567-7257</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>May</Month></PubDate></JournalIssue><Title>Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</Title><ISOAbbreviation>Infect. Genet. Evol.</ISOAbbreviation></Journal><ArticleTitle>Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.</ArticleTitle><Pagination><MedlinePgn>591-4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.meegid.2010.01.012</ELocationID><Abstract><AbstractText>Polymerase chain reaction based methods are promising tools for the monitoring and evaluation of the Global Program for the Elimination of Lymphatic Filariasis. The currently available PCR methods do not differentiate the DNA of Wuchereria bancrofti or Brugia malayi by a single PCR and hence are cumbersome. Therefore, we designed a single step PCR strategy for differentiating Bancroftian infection from Brugian infection based on a newly identified gene from the W. bancrofti genome, abundant larval transcript-2 (alt-2), which is abundantly expressed. The difference in PCR product sizes generated from the presence or absence of evolutionarily altered tandem repeats in alt-2 intron-3 differentiated W. bancrofti from B. malayi. The analysis was performed on the genomic DNA of microfilariae from a number of patient blood samples or microfilariae positive slides from different Indian geographical regions. The assay gave consistent results, differentiating the two filarial parasite species accurately. This alt-2 intron-3 based PCR assay can be a potential tool for the diagnosis and differentiation of co-infections by lymphatic filarial parasites.</AbstractText><CopyrightInformation>Copyright (c) 2010 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sakthidevi</LastName><ForeName>Moorthy</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre for Biotechnology, Anna University, Guindy, Chennai, Tamil Nadu 600025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murugan</LastName><ForeName>Vadivel</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Hoti</LastName><ForeName>Sugeerappa Laxmanappa</ForeName><Initials>SL</Initials></Author><Author ValidYN="Y"><LastName>Kaliraj</LastName><ForeName>Perumal</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>02</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Infect Genet Evol</MedlineTA><NlmUniqueID>101084138</NlmUniqueID><ISSNLinking>1567-1348</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C521555">ALT-2 protein, Brugia malayi</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018509">DNA, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018509" MajorTopicYN="N">DNA, Helminth</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003937" MajorTopicYN="N">Diagnosis, Differential</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007438" MajorTopicYN="N">Introns</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008957" MajorTopicYN="N">Models, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020080" MajorTopicYN="Y">Tandem Repeat Sequences</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>11</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>01</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>01</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>2</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>2</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20132908</ArticleId><ArticleId IdType="pii">S1567-1348(10)00016-X</ArticleId><ArticleId IdType="doi">10.1016/j.meegid.2010.01.012</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B73 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002B73 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:20132908
|texte= Lymphatic filarial species differentiation using evolutionarily modified tandem repeats: generation of new genetic markers.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:20132908" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |