Molecular characterization of dermal lymphatic endothelial cells from primary lymphedema skin.
Identifieur interne : 002707 ( PubMed/Corpus ); précédent : 002706; suivant : 002708Molecular characterization of dermal lymphatic endothelial cells from primary lymphedema skin.
Auteurs : Samuel Ogunbiyi ; Ganessen Chinien ; Della Field ; Julia Humphries ; Kevin Burand ; Barbara Sawyer ; Steve Jeffrey ; Peter Mortimer ; Steve Clasper ; Dave Jackson ; Alberto SmithSource :
- Lymphatic research and biology [ 1557-8585 ] ; 2011.
English descriptors
- KwdEn :
- Adult, Aged, Cell Proliferation (drug effects), Cells, Cultured, Endothelial Cells (cytology), Endothelial Cells (metabolism), Endothelial Cells (pathology), Female, Gene Expression Profiling, Gene Expression Regulation, Humans, Lymphedema (congenital), Lymphedema (genetics), Lymphedema (metabolism), Lymphedema (pathology), Male, Middle Aged, Skin (cytology), Skin (metabolism), Skin (pathology), Vascular Endothelial Growth Factors (pharmacology).
- MESH :
- chemical , pharmacology : Vascular Endothelial Growth Factors.
- congenital : Lymphedema.
- cytology : Endothelial Cells, Skin.
- drug effects : Cell Proliferation.
- genetics : Lymphedema.
- metabolism : Endothelial Cells, Lymphedema, Skin.
- pathology : Endothelial Cells, Lymphedema, Skin.
- Adult, Aged, Cells, Cultured, Female, Gene Expression Profiling, Gene Expression Regulation, Humans, Male, Middle Aged.
Abstract
Lymphatic endothelial cells from primary lymphedema skin have never been cultured nor characterized. A subgroup of patients with primary lymphedema undergo surgery to bring about an improvement in their quality of life. The aim of this study was to culture and characterize LECs from the skin of these patients.
DOI: 10.1089/lrb.2010.0019
PubMed: 21417764
Links to Exploration step
pubmed:21417764Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular characterization of dermal lymphatic endothelial cells from primary lymphedema skin.</title><author><name sortKey="Ogunbiyi, Samuel" sort="Ogunbiyi, Samuel" uniqKey="Ogunbiyi S" first="Samuel" last="Ogunbiyi">Samuel Ogunbiyi</name><affiliation><nlm:affiliation>Academic Department of Surgery, BHF Centre of Research Excellence and NIMR Biomedical Research Centre at Kings Health Partners, St. Thomas Hospital, London, United Kingdom. samuelogunbiyi@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Chinien, Ganessen" sort="Chinien, Ganessen" uniqKey="Chinien G" first="Ganessen" last="Chinien">Ganessen Chinien</name></author><author><name sortKey="Field, Della" sort="Field, Della" uniqKey="Field D" first="Della" last="Field">Della Field</name></author><author><name sortKey="Humphries, Julia" sort="Humphries, Julia" uniqKey="Humphries J" first="Julia" last="Humphries">Julia Humphries</name></author><author><name sortKey="Burand, Kevin" sort="Burand, Kevin" uniqKey="Burand K" first="Kevin" last="Burand">Kevin Burand</name></author><author><name sortKey="Sawyer, Barbara" sort="Sawyer, Barbara" uniqKey="Sawyer B" first="Barbara" last="Sawyer">Barbara Sawyer</name></author><author><name sortKey="Jeffrey, Steve" sort="Jeffrey, Steve" uniqKey="Jeffrey S" first="Steve" last="Jeffrey">Steve Jeffrey</name></author><author><name sortKey="Mortimer, Peter" sort="Mortimer, Peter" uniqKey="Mortimer P" first="Peter" last="Mortimer">Peter Mortimer</name></author><author><name sortKey="Clasper, Steve" sort="Clasper, Steve" uniqKey="Clasper S" first="Steve" last="Clasper">Steve Clasper</name></author><author><name sortKey="Jackson, Dave" sort="Jackson, Dave" uniqKey="Jackson D" first="Dave" last="Jackson">Dave Jackson</name></author><author><name sortKey="Smith, Alberto" sort="Smith, Alberto" uniqKey="Smith A" first="Alberto" last="Smith">Alberto Smith</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21417764</idno><idno type="pmid">21417764</idno><idno type="doi">10.1089/lrb.2010.0019</idno><idno type="wicri:Area/PubMed/Corpus">002707</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002707</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular characterization of dermal lymphatic endothelial cells from primary lymphedema skin.</title><author><name sortKey="Ogunbiyi, Samuel" sort="Ogunbiyi, Samuel" uniqKey="Ogunbiyi S" first="Samuel" last="Ogunbiyi">Samuel Ogunbiyi</name><affiliation><nlm:affiliation>Academic Department of Surgery, BHF Centre of Research Excellence and NIMR Biomedical Research Centre at Kings Health Partners, St. Thomas Hospital, London, United Kingdom. samuelogunbiyi@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Chinien, Ganessen" sort="Chinien, Ganessen" uniqKey="Chinien G" first="Ganessen" last="Chinien">Ganessen Chinien</name></author><author><name sortKey="Field, Della" sort="Field, Della" uniqKey="Field D" first="Della" last="Field">Della Field</name></author><author><name sortKey="Humphries, Julia" sort="Humphries, Julia" uniqKey="Humphries J" first="Julia" last="Humphries">Julia Humphries</name></author><author><name sortKey="Burand, Kevin" sort="Burand, Kevin" uniqKey="Burand K" first="Kevin" last="Burand">Kevin Burand</name></author><author><name sortKey="Sawyer, Barbara" sort="Sawyer, Barbara" uniqKey="Sawyer B" first="Barbara" last="Sawyer">Barbara Sawyer</name></author><author><name sortKey="Jeffrey, Steve" sort="Jeffrey, Steve" uniqKey="Jeffrey S" first="Steve" last="Jeffrey">Steve Jeffrey</name></author><author><name sortKey="Mortimer, Peter" sort="Mortimer, Peter" uniqKey="Mortimer P" first="Peter" last="Mortimer">Peter Mortimer</name></author><author><name sortKey="Clasper, Steve" sort="Clasper, Steve" uniqKey="Clasper S" first="Steve" last="Clasper">Steve Clasper</name></author><author><name sortKey="Jackson, Dave" sort="Jackson, Dave" uniqKey="Jackson D" first="Dave" last="Jackson">Dave Jackson</name></author><author><name sortKey="Smith, Alberto" sort="Smith, Alberto" uniqKey="Smith A" first="Alberto" last="Smith">Alberto Smith</name></author></analytic><series><title level="j">Lymphatic research and biology</title><idno type="eISSN">1557-8585</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Cell Proliferation (drug effects)</term><term>Cells, Cultured</term><term>Endothelial Cells (cytology)</term><term>Endothelial Cells (metabolism)</term><term>Endothelial Cells (pathology)</term><term>Female</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation</term><term>Humans</term><term>Lymphedema (congenital)</term><term>Lymphedema (genetics)</term><term>Lymphedema (metabolism)</term><term>Lymphedema (pathology)</term><term>Male</term><term>Middle Aged</term><term>Skin (cytology)</term><term>Skin (metabolism)</term><term>Skin (pathology)</term><term>Vascular Endothelial Growth Factors (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Vascular Endothelial Growth Factors</term></keywords><keywords scheme="MESH" qualifier="congenital" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Endothelial Cells</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endothelial Cells</term><term>Lymphedema</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Endothelial Cells</term><term>Lymphedema</term><term>Skin</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Cells, Cultured</term><term>Female</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation</term><term>Humans</term><term>Male</term><term>Middle Aged</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic endothelial cells from primary lymphedema skin have never been cultured nor characterized. A subgroup of patients with primary lymphedema undergo surgery to bring about an improvement in their quality of life. The aim of this study was to culture and characterize LECs from the skin of these patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21417764</PMID><DateCreated><Year>2011</Year><Month>03</Month><Day>22</Day></DateCreated><DateCompleted><Year>2011</Year><Month>08</Month><Day>11</Day></DateCompleted><DateRevised><Year>2017</Year><Month>09</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1557-8585</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Lymphatic research and biology</Title><ISOAbbreviation>Lymphat Res Biol</ISOAbbreviation></Journal><ArticleTitle>Molecular characterization of dermal lymphatic endothelial cells from primary lymphedema skin.</ArticleTitle><Pagination><MedlinePgn>19-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1089/lrb.2010.0019</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Lymphatic endothelial cells from primary lymphedema skin have never been cultured nor characterized. A subgroup of patients with primary lymphedema undergo surgery to bring about an improvement in their quality of life. The aim of this study was to culture and characterize LECs from the skin of these patients.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Lymphatic endothelial cells were isolated and cultured from the skin of patients with primary lymphedema and from normal skin. The isolated cells were compared in their ability to form microvascular networks in a three-dimensional culture medium, and in their response to treatment with vascular endothelial growth factors A, C, and D. Whole tissue transcriptional profiling was carried out on two pools of isolated lymphatic endothelial cells--one from primary lymphedema skin and the other from normal skin. Lymphatic endothelial cells from primary lymphedema skin form tubule-like structures when cultured in three-dimensional media. They respond in a similar fashion to stimulation with the vascular endothelial growth factors A, C, and D. Comparative analysis between lymphedema tissue and normal tissue (fold change >2) showed differential expression of 2793 genes (5% of all transcripts), 2184 upregulated, and 609 downregulated. Genes involved in cellular apoptosis (vascular endothelial growth inhibitor, zinc finger protein), extracellular matrix turnover (matrix metalloproteinase inhibitor-16), and type IV collagen deposition were upregulated. Various pro-inflammatory genes (interleukin-6, interleukin-8, interleukin-32, E-selectin) were downregulated.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Cellular adhesion, apoptosis, and increased extracellular matrix turnover play a more prominent role in primary lymphedema than previously thought. In addition, the acute inflammatory response is attenuated as evidenced by the downregulation of various pro-inflammatory genes.This sheds further light on the interplay of the various pathological processes taking place in primary lymphedema.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ogunbiyi</LastName><ForeName>Samuel</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Academic Department of Surgery, BHF Centre of Research Excellence and NIMR Biomedical Research Centre at Kings Health Partners, St. Thomas Hospital, London, United Kingdom. samuelogunbiyi@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chinien</LastName><ForeName>Ganessen</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Field</LastName><ForeName>Della</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Humphries</LastName><ForeName>Julia</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Burand</LastName><ForeName>Kevin</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Sawyer</LastName><ForeName>Barbara</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Jeffrey</LastName><ForeName>Steve</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Mortimer</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Clasper</LastName><ForeName>Steve</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Jackson</LastName><ForeName>Dave</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Alberto</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><CollectiveName>London Lymphedema Consortium</CollectiveName></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>MRC_G1100134</GrantID><Agency>Medical Research Council</Agency><Country>United Kingdom</Country></Grant><Grant><GrantID>MRC_MC_U137884182</GrantID><Agency>Medical Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Lymphat Res Biol</MedlineTA><NlmUniqueID>101163587</NlmUniqueID><ISSNLinking>1539-6851</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D042442">Vascular Endothelial Growth Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042783" MajorTopicYN="N">Endothelial Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008209" MajorTopicYN="N">Lymphedema</DescriptorName><QualifierName UI="Q000151" MajorTopicYN="N">congenital</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012867" MajorTopicYN="N">Skin</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042442" MajorTopicYN="N">Vascular Endothelial Growth Factors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>8</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21417764</ArticleId><ArticleId IdType="doi">10.1089/lrb.2010.0019</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002707 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002707 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:21417764
|texte= Molecular characterization of dermal lymphatic endothelial cells from primary lymphedema skin.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:21417764" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |