Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.
Identifieur interne : 002377 ( PubMed/Corpus ); précédent : 002376; suivant : 002378Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.
Auteurs : Tine V. Karlsen ; Emmet Mccormack ; Maja Mujic ; Olav Tenstad ; Helge WiigSource :
- American journal of physiology. Heart and circulatory physiology [ 1522-1539 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Albumins.
- physiology : Lymph, Lymphatic System.
- Animals, Female, Fluorescent Dyes, Mice, Radiopharmaceuticals, Rats.
Abstract
There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and subsequent calculation of removal rate constants (k). BSA was conjugated with Alexa680 NHS ester and remained stable in protein-rich solutions without free dye dissociation. To assess lymph flow, mice or rats were imaged every 30 or 60 min during a 3- to 6-h period following an intradermal injection of 0.5 or 1 μl Alexa680-albumin. Mice were awake between measurements, whereas rats were anesthetized throughout the experiment. The k, a parameter defined as equivalent to lymph flow, was calculated from the slopes of the resultant log-linear washout curves and averaged -0.40 ± 0.03 and -0.30 ± 0.02%/min for control C57BL/6 and C3H mice, respectively. Local administration of the vasoconstrictor endothelin-1 in mice led to a significant reduction in k, whereas overhydration in rats increased k, reflecting the coupling between capillary filtration and lymph flow. Furthermore, k was 50% of wild type in lymphedema Chy mice where dermal lymphatics are absent. We conclude that lymph flow can be determined as its rate constant k by optical imaging of depot clearance of submicroliter amounts of Alexa680-albumin. The method offers a minimally invasive, reproducible, and simple alternative to assess lymphatic function in mice and rats.
DOI: 10.1152/ajpheart.00842.2011
PubMed: 22101523
Links to Exploration step
pubmed:22101523Le document en format XML
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Karlsen</name><affiliation><nlm:affiliation>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen, Norway. tine.karlsen@biomed.uib.no</nlm:affiliation></affiliation></author><author><name sortKey="Mccormack, Emmet" sort="Mccormack, Emmet" uniqKey="Mccormack E" first="Emmet" last="Mccormack">Emmet Mccormack</name></author><author><name sortKey="Mujic, Maja" sort="Mujic, Maja" uniqKey="Mujic M" first="Maja" last="Mujic">Maja Mujic</name></author><author><name sortKey="Tenstad, Olav" sort="Tenstad, Olav" uniqKey="Tenstad O" first="Olav" last="Tenstad">Olav Tenstad</name></author><author><name sortKey="Wiig, Helge" sort="Wiig, Helge" uniqKey="Wiig H" first="Helge" last="Wiig">Helge Wiig</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22101523</idno><idno type="pmid">22101523</idno><idno type="doi">10.1152/ajpheart.00842.2011</idno><idno type="wicri:Area/PubMed/Corpus">002377</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002377</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.</title><author><name sortKey="Karlsen, Tine V" sort="Karlsen, Tine V" uniqKey="Karlsen T" first="Tine V" last="Karlsen">Tine V. Karlsen</name><affiliation><nlm:affiliation>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen, Norway. tine.karlsen@biomed.uib.no</nlm:affiliation></affiliation></author><author><name sortKey="Mccormack, Emmet" sort="Mccormack, Emmet" uniqKey="Mccormack E" first="Emmet" last="Mccormack">Emmet Mccormack</name></author><author><name sortKey="Mujic, Maja" sort="Mujic, Maja" uniqKey="Mujic M" first="Maja" last="Mujic">Maja Mujic</name></author><author><name sortKey="Tenstad, Olav" sort="Tenstad, Olav" uniqKey="Tenstad O" first="Olav" last="Tenstad">Olav Tenstad</name></author><author><name sortKey="Wiig, Helge" sort="Wiig, Helge" uniqKey="Wiig H" first="Helge" last="Wiig">Helge Wiig</name></author></analytic><series><title level="j">American journal of physiology. Heart and circulatory physiology</title><idno type="eISSN">1522-1539</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Albumins (metabolism)</term><term>Animals</term><term>Female</term><term>Fluorescent Dyes</term><term>Lymph (physiology)</term><term>Lymphatic System (physiology)</term><term>Mice</term><term>Radiopharmaceuticals</term><term>Rats</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Albumins</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lymph</term><term>Lymphatic System</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Fluorescent Dyes</term><term>Mice</term><term>Radiopharmaceuticals</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and subsequent calculation of removal rate constants (k). BSA was conjugated with Alexa680 NHS ester and remained stable in protein-rich solutions without free dye dissociation. To assess lymph flow, mice or rats were imaged every 30 or 60 min during a 3- to 6-h period following an intradermal injection of 0.5 or 1 μl Alexa680-albumin. Mice were awake between measurements, whereas rats were anesthetized throughout the experiment. The k, a parameter defined as equivalent to lymph flow, was calculated from the slopes of the resultant log-linear washout curves and averaged -0.40 ± 0.03 and -0.30 ± 0.02%/min for control C57BL/6 and C3H mice, respectively. Local administration of the vasoconstrictor endothelin-1 in mice led to a significant reduction in k, whereas overhydration in rats increased k, reflecting the coupling between capillary filtration and lymph flow. Furthermore, k was 50% of wild type in lymphedema Chy mice where dermal lymphatics are absent. We conclude that lymph flow can be determined as its rate constant k by optical imaging of depot clearance of submicroliter amounts of Alexa680-albumin. The method offers a minimally invasive, reproducible, and simple alternative to assess lymphatic function in mice and rats.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22101523</PMID><DateCreated><Year>2012</Year><Month>01</Month><Day>04</Day></DateCreated><DateCompleted><Year>2012</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2012</Year><Month>01</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1522-1539</ISSN><JournalIssue CitedMedium="Internet"><Volume>302</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Jan</Month></PubDate></JournalIssue><Title>American journal of physiology. Heart and circulatory physiology</Title><ISOAbbreviation>Am. J. Physiol. Heart Circ. Physiol.</ISOAbbreviation></Journal><ArticleTitle>Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.</ArticleTitle><Pagination><MedlinePgn>H391-401</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1152/ajpheart.00842.2011</ELocationID><Abstract><AbstractText>There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and subsequent calculation of removal rate constants (k). BSA was conjugated with Alexa680 NHS ester and remained stable in protein-rich solutions without free dye dissociation. To assess lymph flow, mice or rats were imaged every 30 or 60 min during a 3- to 6-h period following an intradermal injection of 0.5 or 1 μl Alexa680-albumin. Mice were awake between measurements, whereas rats were anesthetized throughout the experiment. The k, a parameter defined as equivalent to lymph flow, was calculated from the slopes of the resultant log-linear washout curves and averaged -0.40 ± 0.03 and -0.30 ± 0.02%/min for control C57BL/6 and C3H mice, respectively. Local administration of the vasoconstrictor endothelin-1 in mice led to a significant reduction in k, whereas overhydration in rats increased k, reflecting the coupling between capillary filtration and lymph flow. Furthermore, k was 50% of wild type in lymphedema Chy mice where dermal lymphatics are absent. We conclude that lymph flow can be determined as its rate constant k by optical imaging of depot clearance of submicroliter amounts of Alexa680-albumin. The method offers a minimally invasive, reproducible, and simple alternative to assess lymphatic function in mice and rats.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Karlsen</LastName><ForeName>Tine V</ForeName><Initials>TV</Initials><AffiliationInfo><Affiliation>Department of Biomedicine, Hematology Section, University of Bergen, Jonas Lies Vei 91, Bergen, Norway. tine.karlsen@biomed.uib.no</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McCormack</LastName><ForeName>Emmet</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Mujic</LastName><ForeName>Maja</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tenstad</LastName><ForeName>Olav</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Wiig</LastName><ForeName>Helge</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>11</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Am J Physiol Heart Circ Physiol</MedlineTA><NlmUniqueID>100901228</NlmUniqueID><ISSNLinking>0363-6135</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000418">Albumins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019275">Radiopharmaceuticals</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000418" MajorTopicYN="N">Albumins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008196" MajorTopicYN="N">Lymph</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008208" MajorTopicYN="N">Lymphatic System</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019275" MajorTopicYN="N">Radiopharmaceuticals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22101523</ArticleId><ArticleId IdType="pii">ajpheart.00842.2011</ArticleId><ArticleId IdType="doi">10.1152/ajpheart.00842.2011</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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