Improved regeneration of autologous transplanted lymph node fragments by VEGF-C treatment.
Identifieur interne : 002223 ( PubMed/Corpus ); précédent : 002222; suivant : 002224Improved regeneration of autologous transplanted lymph node fragments by VEGF-C treatment.
Auteurs : Tanja Sommer ; Manuela Buettner ; Frank Bruns ; Gerhard Breves ; Catarina Hadamitzky ; Reinhard PabstSource :
- Anatomical record (Hoboken, N.J. : 2007) [ 1932-8494 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Female, Injections, Subcutaneous, Lymph Node Excision (adverse effects), Lymph Nodes (drug effects), Lymph Nodes (physiopathology), Lymph Nodes (transplantation), Lymphangiogenesis (drug effects), Lymphedema (etiology), Lymphedema (physiopathology), Lymphedema (prevention & control), Radiotherapy, Adjuvant (adverse effects), Rats, Rats, Inbred Lew, Regeneration (drug effects), Time Factors, Transplantation, Autologous, Vascular Endothelial Growth Factor C (administration & dosage), Vascular Endothelial Growth Factor C (pharmacology).
- MESH :
- chemical , administration & dosage : Vascular Endothelial Growth Factor C.
- adverse effects : Lymph Node Excision, Radiotherapy, Adjuvant.
- drug effects : Lymph Nodes, Lymphangiogenesis, Regeneration.
- etiology : Lymphedema.
- chemical , pharmacology : Vascular Endothelial Growth Factor C.
- physiopathology : Lymph Nodes, Lymphedema.
- prevention & control : Lymphedema.
- transplantation : Lymph Nodes.
- Animals, Female, Injections, Subcutaneous, Rats, Rats, Inbred Lew, Time Factors, Transplantation, Autologous.
Abstract
Secondary lymphedema is a common complication after removal of lymph nodes in combination with radiation therapy in the treatment of breast cancer, cervical cancer, and melanomas. Only symptomatic therapies are available at the moment, and lymphedema is for most patients a lifelong condition involving psychological and physical disabilities. Animal models exist to study the pathophysiology of lymphedema but not to study surgical treatments. The aim of this study was to show that regeneration of autologous transplanted lymph node fragments is possible in rats that were irradiated previously locally in the groin and to examine the effects of vascular endothelial growth factor (VEGF)-C injections on the rate of regeneration of transplanted lymph nodes. In all of the animals, inguinal and popliteal lymph nodes and adjacent lymphatic vessels were unilaterally removed and the inguinal region irradiated by a single dose of 15 Gy. Afterward, lymph node fragments were transplanted subcutaneously in the irradiated region. Half of the animals were treated by local VEGF-C injections after transplantation. Four weeks after transplantation, drainage of the leg was tested by injection of blue dye, and the transplanted fragments were removed and examined immunohistologically. We could show that regeneration of autologous transplanted lymph node fragments is possible in areas treated with radiotherapy in the rat. We also documented that transplants can achieve a connection to the lymphatic collectors of the leg. The results suggest that the outcome of regeneration can be improved by injection of VEGF-C in the transplantation area. Thus, lymph node fragment regeneration may be relevant for lymphedema prevention and therapy.
DOI: 10.1002/ar.22438
PubMed: 22419614
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pubmed:22419614Le document en format XML
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Hadamitzky</name></author><author><name sortKey="Pabst, Reinhard" sort="Pabst, Reinhard" uniqKey="Pabst R" first="Reinhard" last="Pabst">Reinhard Pabst</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22419614</idno><idno type="pmid">22419614</idno><idno type="doi">10.1002/ar.22438</idno><idno type="wicri:Area/PubMed/Corpus">002223</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002223</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Improved regeneration of autologous transplanted lymph node fragments by VEGF-C treatment.</title><author><name sortKey="Sommer, Tanja" sort="Sommer, Tanja" uniqKey="Sommer T" first="Tanja" last="Sommer">Tanja Sommer</name><affiliation><nlm:affiliation>Institute of Immunomorphology, Hannover Medical School, Hannover, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Buettner, Manuela" sort="Buettner, Manuela" uniqKey="Buettner M" first="Manuela" last="Buettner">Manuela Buettner</name></author><author><name sortKey="Bruns, Frank" sort="Bruns, Frank" uniqKey="Bruns F" first="Frank" last="Bruns">Frank Bruns</name></author><author><name sortKey="Breves, Gerhard" sort="Breves, Gerhard" uniqKey="Breves G" first="Gerhard" last="Breves">Gerhard Breves</name></author><author><name sortKey="Hadamitzky, Catarina" sort="Hadamitzky, Catarina" uniqKey="Hadamitzky C" first="Catarina" last="Hadamitzky">Catarina Hadamitzky</name></author><author><name sortKey="Pabst, Reinhard" sort="Pabst, Reinhard" uniqKey="Pabst R" first="Reinhard" last="Pabst">Reinhard Pabst</name></author></analytic><series><title level="j">Anatomical record (Hoboken, N.J. : 2007)</title><idno type="eISSN">1932-8494</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" 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xml:lang="en"><term>Lymph Node Excision</term><term>Radiotherapy, Adjuvant</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lymph Nodes</term><term>Lymphangiogenesis</term><term>Regeneration</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Vascular Endothelial Growth Factor C</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Lymph Nodes</term><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Lymph Nodes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Injections, Subcutaneous</term><term>Rats</term><term>Rats, Inbred Lew</term><term>Time Factors</term><term>Transplantation, Autologous</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Secondary lymphedema is a common complication after removal of lymph nodes in combination with radiation therapy in the treatment of breast cancer, cervical cancer, and melanomas. Only symptomatic therapies are available at the moment, and lymphedema is for most patients a lifelong condition involving psychological and physical disabilities. Animal models exist to study the pathophysiology of lymphedema but not to study surgical treatments. The aim of this study was to show that regeneration of autologous transplanted lymph node fragments is possible in rats that were irradiated previously locally in the groin and to examine the effects of vascular endothelial growth factor (VEGF)-C injections on the rate of regeneration of transplanted lymph nodes. In all of the animals, inguinal and popliteal lymph nodes and adjacent lymphatic vessels were unilaterally removed and the inguinal region irradiated by a single dose of 15 Gy. Afterward, lymph node fragments were transplanted subcutaneously in the irradiated region. Half of the animals were treated by local VEGF-C injections after transplantation. Four weeks after transplantation, drainage of the leg was tested by injection of blue dye, and the transplanted fragments were removed and examined immunohistologically. We could show that regeneration of autologous transplanted lymph node fragments is possible in areas treated with radiotherapy in the rat. We also documented that transplants can achieve a connection to the lymphatic collectors of the leg. The results suggest that the outcome of regeneration can be improved by injection of VEGF-C in the transplantation area. Thus, lymph node fragment regeneration may be relevant for lymphedema prevention and therapy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22419614</PMID><DateCreated><Year>2012</Year><Month>04</Month><Day>12</Day></DateCreated><DateCompleted><Year>2012</Year><Month>08</Month><Day>27</Day></DateCompleted><DateRevised><Year>2012</Year><Month>04</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-8494</ISSN><JournalIssue CitedMedium="Internet"><Volume>295</Volume><Issue>5</Issue><PubDate><Year>2012</Year><Month>May</Month></PubDate></JournalIssue><Title>Anatomical record (Hoboken, N.J. : 2007)</Title><ISOAbbreviation>Anat Rec (Hoboken)</ISOAbbreviation></Journal><ArticleTitle>Improved regeneration of autologous transplanted lymph node fragments by VEGF-C treatment.</ArticleTitle><Pagination><MedlinePgn>786-91</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/ar.22438</ELocationID><Abstract><AbstractText>Secondary lymphedema is a common complication after removal of lymph nodes in combination with radiation therapy in the treatment of breast cancer, cervical cancer, and melanomas. Only symptomatic therapies are available at the moment, and lymphedema is for most patients a lifelong condition involving psychological and physical disabilities. Animal models exist to study the pathophysiology of lymphedema but not to study surgical treatments. The aim of this study was to show that regeneration of autologous transplanted lymph node fragments is possible in rats that were irradiated previously locally in the groin and to examine the effects of vascular endothelial growth factor (VEGF)-C injections on the rate of regeneration of transplanted lymph nodes. In all of the animals, inguinal and popliteal lymph nodes and adjacent lymphatic vessels were unilaterally removed and the inguinal region irradiated by a single dose of 15 Gy. Afterward, lymph node fragments were transplanted subcutaneously in the irradiated region. Half of the animals were treated by local VEGF-C injections after transplantation. Four weeks after transplantation, drainage of the leg was tested by injection of blue dye, and the transplanted fragments were removed and examined immunohistologically. We could show that regeneration of autologous transplanted lymph node fragments is possible in areas treated with radiotherapy in the rat. We also documented that transplants can achieve a connection to the lymphatic collectors of the leg. The results suggest that the outcome of regeneration can be improved by injection of VEGF-C in the transplantation area. Thus, lymph node fragment regeneration may be relevant for lymphedema prevention and therapy.</AbstractText><CopyrightInformation>Copyright © 2012 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sommer</LastName><ForeName>Tanja</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Immunomorphology, Hannover Medical School, Hannover, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Buettner</LastName><ForeName>Manuela</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bruns</LastName><ForeName>Frank</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Breves</LastName><ForeName>Gerhard</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Hadamitzky</LastName><ForeName>Catarina</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Pabst</LastName><ForeName>Reinhard</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>03</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anat Rec (Hoboken)</MedlineTA><NlmUniqueID>101292775</NlmUniqueID><ISSNLinking>1932-8486</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D042582">Vascular Endothelial Growth Factor C</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007279" MajorTopicYN="N">Injections, Subcutaneous</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008197" MajorTopicYN="N">Lymph Node Excision</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008198" MajorTopicYN="N">Lymph Nodes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName><QualifierName UI="Q000637" MajorTopicYN="Y">transplantation</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042583" MajorTopicYN="N">Lymphangiogenesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008209" MajorTopicYN="N">Lymphedema</DescriptorName><QualifierName UI="Q000209" 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