Improved regeneration of autologous transplanted lymph node fragments by VEGF-C treatment.
Identifieur interne : 002223 ( PubMed/Corpus ); précédent : 002222; suivant : 002224Improved regeneration of autologous transplanted lymph node fragments by VEGF-C treatment.
Auteurs : Tanja Sommer ; Manuela Buettner ; Frank Bruns ; Gerhard Breves ; Catarina Hadamitzky ; Reinhard PabstSource :
- Anatomical record (Hoboken, N.J. : 2007) [ 1932-8494 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Female, Injections, Subcutaneous, Lymph Node Excision (adverse effects), Lymph Nodes (drug effects), Lymph Nodes (physiopathology), Lymph Nodes (transplantation), Lymphangiogenesis (drug effects), Lymphedema (etiology), Lymphedema (physiopathology), Lymphedema (prevention & control), Radiotherapy, Adjuvant (adverse effects), Rats, Rats, Inbred Lew, Regeneration (drug effects), Time Factors, Transplantation, Autologous, Vascular Endothelial Growth Factor C (administration & dosage), Vascular Endothelial Growth Factor C (pharmacology).
- MESH :
- chemical , administration & dosage : Vascular Endothelial Growth Factor C.
- adverse effects : Lymph Node Excision, Radiotherapy, Adjuvant.
- drug effects : Lymph Nodes, Lymphangiogenesis, Regeneration.
- etiology : Lymphedema.
- chemical , pharmacology : Vascular Endothelial Growth Factor C.
- physiopathology : Lymph Nodes, Lymphedema.
- prevention & control : Lymphedema.
- transplantation : Lymph Nodes.
- Animals, Female, Injections, Subcutaneous, Rats, Rats, Inbred Lew, Time Factors, Transplantation, Autologous.
Abstract
Secondary lymphedema is a common complication after removal of lymph nodes in combination with radiation therapy in the treatment of breast cancer, cervical cancer, and melanomas. Only symptomatic therapies are available at the moment, and lymphedema is for most patients a lifelong condition involving psychological and physical disabilities. Animal models exist to study the pathophysiology of lymphedema but not to study surgical treatments. The aim of this study was to show that regeneration of autologous transplanted lymph node fragments is possible in rats that were irradiated previously locally in the groin and to examine the effects of vascular endothelial growth factor (VEGF)-C injections on the rate of regeneration of transplanted lymph nodes. In all of the animals, inguinal and popliteal lymph nodes and adjacent lymphatic vessels were unilaterally removed and the inguinal region irradiated by a single dose of 15 Gy. Afterward, lymph node fragments were transplanted subcutaneously in the irradiated region. Half of the animals were treated by local VEGF-C injections after transplantation. Four weeks after transplantation, drainage of the leg was tested by injection of blue dye, and the transplanted fragments were removed and examined immunohistologically. We could show that regeneration of autologous transplanted lymph node fragments is possible in areas treated with radiotherapy in the rat. We also documented that transplants can achieve a connection to the lymphatic collectors of the leg. The results suggest that the outcome of regeneration can be improved by injection of VEGF-C in the transplantation area. Thus, lymph node fragment regeneration may be relevant for lymphedema prevention and therapy.
DOI: 10.1002/ar.22438
PubMed: 22419614
Links to Exploration step
pubmed:22419614Le document en format XML
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<author><name sortKey="Sommer, Tanja" sort="Sommer, Tanja" uniqKey="Sommer T" first="Tanja" last="Sommer">Tanja Sommer</name>
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<author><name sortKey="Buettner, Manuela" sort="Buettner, Manuela" uniqKey="Buettner M" first="Manuela" last="Buettner">Manuela Buettner</name>
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<author><name sortKey="Bruns, Frank" sort="Bruns, Frank" uniqKey="Bruns F" first="Frank" last="Bruns">Frank Bruns</name>
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<author><name sortKey="Breves, Gerhard" sort="Breves, Gerhard" uniqKey="Breves G" first="Gerhard" last="Breves">Gerhard Breves</name>
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<author><name sortKey="Hadamitzky, Catarina" sort="Hadamitzky, Catarina" uniqKey="Hadamitzky C" first="Catarina" last="Hadamitzky">Catarina Hadamitzky</name>
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<term>Lymph Nodes (drug effects)</term>
<term>Lymph Nodes (physiopathology)</term>
<term>Lymph Nodes (transplantation)</term>
<term>Lymphangiogenesis (drug effects)</term>
<term>Lymphedema (etiology)</term>
<term>Lymphedema (physiopathology)</term>
<term>Lymphedema (prevention & control)</term>
<term>Radiotherapy, Adjuvant (adverse effects)</term>
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<term>Rats, Inbred Lew</term>
<term>Regeneration (drug effects)</term>
<term>Time Factors</term>
<term>Transplantation, Autologous</term>
<term>Vascular Endothelial Growth Factor C (administration & dosage)</term>
<term>Vascular Endothelial Growth Factor C (pharmacology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vascular Endothelial Growth Factor C</term>
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<keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Lymph Node Excision</term>
<term>Radiotherapy, Adjuvant</term>
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<term>Regeneration</term>
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<term>Female</term>
<term>Injections, Subcutaneous</term>
<term>Rats</term>
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<front><div type="abstract" xml:lang="en">Secondary lymphedema is a common complication after removal of lymph nodes in combination with radiation therapy in the treatment of breast cancer, cervical cancer, and melanomas. Only symptomatic therapies are available at the moment, and lymphedema is for most patients a lifelong condition involving psychological and physical disabilities. Animal models exist to study the pathophysiology of lymphedema but not to study surgical treatments. The aim of this study was to show that regeneration of autologous transplanted lymph node fragments is possible in rats that were irradiated previously locally in the groin and to examine the effects of vascular endothelial growth factor (VEGF)-C injections on the rate of regeneration of transplanted lymph nodes. In all of the animals, inguinal and popliteal lymph nodes and adjacent lymphatic vessels were unilaterally removed and the inguinal region irradiated by a single dose of 15 Gy. Afterward, lymph node fragments were transplanted subcutaneously in the irradiated region. Half of the animals were treated by local VEGF-C injections after transplantation. Four weeks after transplantation, drainage of the leg was tested by injection of blue dye, and the transplanted fragments were removed and examined immunohistologically. We could show that regeneration of autologous transplanted lymph node fragments is possible in areas treated with radiotherapy in the rat. We also documented that transplants can achieve a connection to the lymphatic collectors of the leg. The results suggest that the outcome of regeneration can be improved by injection of VEGF-C in the transplantation area. Thus, lymph node fragment regeneration may be relevant for lymphedema prevention and therapy.</div>
</front>
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<Abstract><AbstractText>Secondary lymphedema is a common complication after removal of lymph nodes in combination with radiation therapy in the treatment of breast cancer, cervical cancer, and melanomas. Only symptomatic therapies are available at the moment, and lymphedema is for most patients a lifelong condition involving psychological and physical disabilities. Animal models exist to study the pathophysiology of lymphedema but not to study surgical treatments. The aim of this study was to show that regeneration of autologous transplanted lymph node fragments is possible in rats that were irradiated previously locally in the groin and to examine the effects of vascular endothelial growth factor (VEGF)-C injections on the rate of regeneration of transplanted lymph nodes. In all of the animals, inguinal and popliteal lymph nodes and adjacent lymphatic vessels were unilaterally removed and the inguinal region irradiated by a single dose of 15 Gy. Afterward, lymph node fragments were transplanted subcutaneously in the irradiated region. Half of the animals were treated by local VEGF-C injections after transplantation. Four weeks after transplantation, drainage of the leg was tested by injection of blue dye, and the transplanted fragments were removed and examined immunohistologically. We could show that regeneration of autologous transplanted lymph node fragments is possible in areas treated with radiotherapy in the rat. We also documented that transplants can achieve a connection to the lymphatic collectors of the leg. The results suggest that the outcome of regeneration can be improved by injection of VEGF-C in the transplantation area. Thus, lymph node fragment regeneration may be relevant for lymphedema prevention and therapy.</AbstractText>
<CopyrightInformation>Copyright © 2012 Wiley Periodicals, Inc.</CopyrightInformation>
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