Brugia malayi thioredoxin peroxidase as a potential vaccine candidate antigen for lymphatic filariasis.
Identifieur interne : 002153 ( PubMed/Corpus ); précédent : 002152; suivant : 002154Brugia malayi thioredoxin peroxidase as a potential vaccine candidate antigen for lymphatic filariasis.
Auteurs : Setty Balakrishnan Anand ; Vasudevan Rajagopal ; Perumal KalirajSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Antibodies (blood), Antibodies (immunology), Antigens (genetics), Antigens (immunology), Brugia malayi (enzymology), Brugia malayi (genetics), Cell Proliferation, Cytokines (metabolism), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (metabolism), Elephantiasis, Filarial (prevention & control), Endemic Diseases (prevention & control), Humans, Immunity, Cellular, Immunity, Humoral, Male, Mice, Peroxiredoxins (genetics), Peroxiredoxins (immunology), Spleen (cytology), Spleen (immunology), T-Lymphocytes (immunology), Vaccines (genetics), Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies.
- chemical , genetics : Antigens, Peroxiredoxins, Vaccines.
- chemical , immunology : Antibodies, Antigens, Peroxiredoxins, Vaccines.
- cytology : Spleen.
- enzymology : Brugia malayi.
- epidemiology : Elephantiasis, Filarial.
- genetics : Brugia malayi.
- immunology : Elephantiasis, Filarial, Spleen, T-Lymphocytes.
- chemical , metabolism : Cytokines, Elephantiasis, Filarial.
- prevention & control : Elephantiasis, Filarial, Endemic Diseases.
- Animals, Cell Proliferation, Humans, Immunity, Cellular, Immunity, Humoral, Male, Mice.
Abstract
Attempts were made to evaluate the protective efficacy of Brugia malayi thioredoxin peroxidase (BmTPX) in a mouse model. Mice immunized with a protein vaccine containing rBmTPX developed higher titres (1:5,000/1:10,000) of anti-BmTPX antibodies, compared with the mice immunized with the alum control. There was a higher level of cellular proliferative response in mice immunized with BmTPX compared with the alum control (p < 0.05), which was associated with a Th2-type of response. In order to compare the prophylactic efficacy of BmTPX in natural infection, we evaluated the human immune responses to these antigens in endemic normals (EN) and infected individuals (microfilaraemic and chronic pathology). Results showed that EN subjects carry BmTPX-specific IgG1 and IgG3 circulating antibodies against natural exposure to filariasis. Peripheral blood mononuclear cells from EN subjects responded strongly to rBmTPX by proliferating, as well as by secreting interferon (IFN)-γ (Th1) and IL-5 (Th2), a mixed type of response to rBmTPX. In the case of infected individuals, there was no IFN-γ or IL-5 response. Thus, there was a clear dichotomy in the cytokine production by infected versus EN individuals. Our findings suggest that BmTPX may be a suitable antigen candidate for lymphatic filariasis, but a further study is still required.
DOI: 10.1007/s12010-012-9643-6
PubMed: 22528648
Links to Exploration step
pubmed:22528648Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Brugia malayi thioredoxin peroxidase as a potential vaccine candidate antigen for lymphatic filariasis.</title><author><name sortKey="Anand, Setty Balakrishnan" sort="Anand, Setty Balakrishnan" uniqKey="Anand S" first="Setty Balakrishnan" last="Anand">Setty Balakrishnan Anand</name><affiliation><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.</nlm:affiliation></affiliation></author><author><name sortKey="Rajagopal, Vasudevan" sort="Rajagopal, Vasudevan" uniqKey="Rajagopal V" first="Vasudevan" last="Rajagopal">Vasudevan Rajagopal</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22528648</idno><idno type="pmid">22528648</idno><idno type="doi">10.1007/s12010-012-9643-6</idno><idno type="wicri:Area/PubMed/Corpus">002153</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002153</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Brugia malayi thioredoxin peroxidase as a potential vaccine candidate antigen for lymphatic filariasis.</title><author><name sortKey="Anand, Setty Balakrishnan" sort="Anand, Setty Balakrishnan" uniqKey="Anand S" first="Setty Balakrishnan" last="Anand">Setty Balakrishnan Anand</name><affiliation><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.</nlm:affiliation></affiliation></author><author><name sortKey="Rajagopal, Vasudevan" sort="Rajagopal, Vasudevan" uniqKey="Rajagopal V" first="Vasudevan" last="Rajagopal">Vasudevan Rajagopal</name></author><author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies (blood)</term><term>Antibodies (immunology)</term><term>Antigens (genetics)</term><term>Antigens (immunology)</term><term>Brugia malayi (enzymology)</term><term>Brugia malayi (genetics)</term><term>Cell Proliferation</term><term>Cytokines (metabolism)</term><term>Elephantiasis, Filarial (epidemiology)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (metabolism)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Endemic Diseases (prevention & control)</term><term>Humans</term><term>Immunity, Cellular</term><term>Immunity, Humoral</term><term>Male</term><term>Mice</term><term>Peroxiredoxins (genetics)</term><term>Peroxiredoxins (immunology)</term><term>Spleen (cytology)</term><term>Spleen (immunology)</term><term>T-Lymphocytes (immunology)</term><term>Vaccines (genetics)</term><term>Vaccines (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens</term><term>Peroxiredoxins</term><term>Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Antigens</term><term>Peroxiredoxins</term><term>Vaccines</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Spleen</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Endemic Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Proliferation</term><term>Humans</term><term>Immunity, Cellular</term><term>Immunity, Humoral</term><term>Male</term><term>Mice</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Attempts were made to evaluate the protective efficacy of Brugia malayi thioredoxin peroxidase (BmTPX) in a mouse model. Mice immunized with a protein vaccine containing rBmTPX developed higher titres (1:5,000/1:10,000) of anti-BmTPX antibodies, compared with the mice immunized with the alum control. There was a higher level of cellular proliferative response in mice immunized with BmTPX compared with the alum control (p < 0.05), which was associated with a Th2-type of response. In order to compare the prophylactic efficacy of BmTPX in natural infection, we evaluated the human immune responses to these antigens in endemic normals (EN) and infected individuals (microfilaraemic and chronic pathology). Results showed that EN subjects carry BmTPX-specific IgG1 and IgG3 circulating antibodies against natural exposure to filariasis. Peripheral blood mononuclear cells from EN subjects responded strongly to rBmTPX by proliferating, as well as by secreting interferon (IFN)-γ (Th1) and IL-5 (Th2), a mixed type of response to rBmTPX. In the case of infected individuals, there was no IFN-γ or IL-5 response. Thus, there was a clear dichotomy in the cytokine production by infected versus EN individuals. Our findings suggest that BmTPX may be a suitable antigen candidate for lymphatic filariasis, but a further study is still required.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22528648</PMID><DateCreated><Year>2012</Year><Month>07</Month><Day>20</Day></DateCreated><DateCompleted><Year>2012</Year><Month>11</Month><Day>21</Day></DateCompleted><DateRevised><Year>2012</Year><Month>07</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>167</Volume><Issue>5</Issue><PubDate><Year>2012</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl. Biochem. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>Brugia malayi thioredoxin peroxidase as a potential vaccine candidate antigen for lymphatic filariasis.</ArticleTitle><Pagination><MedlinePgn>1351-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-012-9643-6</ELocationID><Abstract><AbstractText>Attempts were made to evaluate the protective efficacy of Brugia malayi thioredoxin peroxidase (BmTPX) in a mouse model. Mice immunized with a protein vaccine containing rBmTPX developed higher titres (1:5,000/1:10,000) of anti-BmTPX antibodies, compared with the mice immunized with the alum control. There was a higher level of cellular proliferative response in mice immunized with BmTPX compared with the alum control (p < 0.05), which was associated with a Th2-type of response. In order to compare the prophylactic efficacy of BmTPX in natural infection, we evaluated the human immune responses to these antigens in endemic normals (EN) and infected individuals (microfilaraemic and chronic pathology). Results showed that EN subjects carry BmTPX-specific IgG1 and IgG3 circulating antibodies against natural exposure to filariasis. Peripheral blood mononuclear cells from EN subjects responded strongly to rBmTPX by proliferating, as well as by secreting interferon (IFN)-γ (Th1) and IL-5 (Th2), a mixed type of response to rBmTPX. In the case of infected individuals, there was no IFN-γ or IL-5 response. Thus, there was a clear dichotomy in the cytokine production by infected versus EN individuals. Our findings suggest that BmTPX may be a suitable antigen candidate for lymphatic filariasis, but a further study is still required.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Anand</LastName><ForeName>Setty Balakrishnan</ForeName><Initials>SB</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rajagopal</LastName><ForeName>Vasudevan</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Kaliraj</LastName><ForeName>Perumal</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>04</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000906">Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000941">Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014612">Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.15</RegistryNumber><NameOfSubstance UI="D054464">Peroxiredoxins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000941" MajorTopicYN="N">Antigens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000378" 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