Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi.
Identifieur interne : 001E97 ( PubMed/Corpus ); précédent : 001E96; suivant : 001E98Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi.
Auteurs : Jeetendra Kumar Nag ; Nidhi Shrivastava ; Jyoti Gupta ; Shailja Misra-BhattacharyaSource :
- Comparative immunology, microbiology and infectious diseases [ 1878-1667 ] ; 2013.
English descriptors
- KwdEn :
- Animals, B-Lymphocytes (immunology), Brugia malayi (immunology), Cell Adhesion (immunology), Cloning, Molecular, Cross Reactions (immunology), Cytokines (immunology), Cytokines (metabolism), Cytotoxicity, Immunologic, Elephantiasis, Filarial (prevention & control), Female, Gene Expression, Histocompatibility Antigens Class II (immunology), Immunoglobulin E (blood), Immunoglobulin E (immunology), Immunoglobulin G (blood), Immunoglobulin G (immunology), Immunoglobulin M (blood), Immunoglobulin M (immunology), Lymphocyte Activation, Macrophage Activation (immunology), Macrophages (immunology), Male, Mice, Murinae, Prokaryotic Initiation Factor-1 (genetics), Prokaryotic Initiation Factor-1 (immunology), Prokaryotic Initiation Factor-1 (secretion), Reactive Oxygen Species (metabolism), Recombinant Proteins (administration & dosage), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Th2 Cells (immunology), Wolbachia (genetics), Wolbachia (immunology).
- MESH :
- chemical , administration & dosage : Recombinant Proteins.
- chemical , blood : Immunoglobulin E, Immunoglobulin G, Immunoglobulin M.
- chemical , genetics : Prokaryotic Initiation Factor-1, Recombinant Proteins.
- chemical , immunology : Cytokines, Histocompatibility Antigens Class II, Immunoglobulin E, Immunoglobulin G, Immunoglobulin M, Prokaryotic Initiation Factor-1, Recombinant Proteins.
- genetics : Wolbachia.
- immunology : B-Lymphocytes, Brugia malayi, Cell Adhesion, Cross Reactions, Macrophage Activation, Macrophages, Th2 Cells, Wolbachia.
- chemical , metabolism : Cytokines, Reactive Oxygen Species.
- prevention & control : Elephantiasis, Filarial.
- chemical , secretion : Prokaryotic Initiation Factor-1.
- Animals, Cloning, Molecular, Cytotoxicity, Immunologic, Female, Gene Expression, Lymphocyte Activation, Male, Mice, Murinae.
Abstract
Wolbachia, the intracellular alpha-proteobacteria are required for the development, fertility and survival of filarial parasites. Wolbachia Translation initiation factor-1 (Wol Tl IF-1) is one of the factors required for Wolbachia growth and viability. In the present study, we cloned, over expressed and purified Wol Tl IF-1 that exhibited strong immuno-reactivity with various categories of bancroftian sera. Immunization with the recombinant protein resulted into significant reduction in microfilarial density (70-72%) and adult worm establishment (61-63%) in susceptible Mastomys coucha. Protection offered by Wol Tl IF-1 was found associated with humoral immune arm as observed by an increased antibody level with preponderance of IgE, IgM, IgG1 and IgG2a isotypes. The anti-Wol Tl IF-1 antibodies promoted profound adherence of peritoneal exudates cells to the surface of microfilariae and infective larvae causing cytotoxicity and their death. The present study indicates potential of recombinant Wol Tl IF-1 as a promising vaccine candidate against human lymphatic filarial infection.
DOI: 10.1016/j.cimid.2012.09.004
PubMed: 23079772
Links to Exploration step
pubmed:23079772Le document en format XML
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last="Misra-Bhattacharya">Shailja Misra-Bhattacharya</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23079772</idno><idno type="pmid">23079772</idno><idno type="doi">10.1016/j.cimid.2012.09.004</idno><idno type="wicri:Area/PubMed/Corpus">001E97</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi.</title><author><name sortKey="Nag, Jeetendra Kumar" sort="Nag, Jeetendra Kumar" uniqKey="Nag J" first="Jeetendra Kumar" last="Nag">Jeetendra Kumar Nag</name><affiliation><nlm:affiliation>Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.</nlm:affiliation></affiliation></author><author><name sortKey="Shrivastava, Nidhi" sort="Shrivastava, Nidhi" uniqKey="Shrivastava N" first="Nidhi" last="Shrivastava">Nidhi Shrivastava</name></author><author><name sortKey="Gupta, Jyoti" sort="Gupta, Jyoti" uniqKey="Gupta J" first="Jyoti" last="Gupta">Jyoti Gupta</name></author><author><name sortKey="Misra Bhattacharya, Shailja" sort="Misra Bhattacharya, Shailja" uniqKey="Misra Bhattacharya S" first="Shailja" last="Misra-Bhattacharya">Shailja Misra-Bhattacharya</name></author></analytic><series><title level="j">Comparative immunology, microbiology and infectious diseases</title><idno type="eISSN">1878-1667</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>B-Lymphocytes (immunology)</term><term>Brugia malayi (immunology)</term><term>Cell Adhesion 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(metabolism)</term><term>Recombinant Proteins (administration & dosage)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Th2 Cells (immunology)</term><term>Wolbachia (genetics)</term><term>Wolbachia (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin E</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Prokaryotic Initiation Factor-1</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Cytokines</term><term>Histocompatibility Antigens Class II</term><term>Immunoglobulin E</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term><term>Prokaryotic Initiation Factor-1</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Brugia malayi</term><term>Cell Adhesion</term><term>Cross Reactions</term><term>Macrophage Activation</term><term>Macrophages</term><term>Th2 Cells</term><term>Wolbachia</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="secretion" xml:lang="en"><term>Prokaryotic Initiation Factor-1</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Cytotoxicity, Immunologic</term><term>Female</term><term>Gene Expression</term><term>Lymphocyte Activation</term><term>Male</term><term>Mice</term><term>Murinae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wolbachia, the intracellular alpha-proteobacteria are required for the development, fertility and survival of filarial parasites. Wolbachia Translation initiation factor-1 (Wol Tl IF-1) is one of the factors required for Wolbachia growth and viability. In the present study, we cloned, over expressed and purified Wol Tl IF-1 that exhibited strong immuno-reactivity with various categories of bancroftian sera. Immunization with the recombinant protein resulted into significant reduction in microfilarial density (70-72%) and adult worm establishment (61-63%) in susceptible Mastomys coucha. Protection offered by Wol Tl IF-1 was found associated with humoral immune arm as observed by an increased antibody level with preponderance of IgE, IgM, IgG1 and IgG2a isotypes. The anti-Wol Tl IF-1 antibodies promoted profound adherence of peritoneal exudates cells to the surface of microfilariae and infective larvae causing cytotoxicity and their death. The present study indicates potential of recombinant Wol Tl IF-1 as a promising vaccine candidate against human lymphatic filarial infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23079772</PMID><DateCreated><Year>2012</Year><Month>12</Month><Day>20</Day></DateCreated><DateCompleted><Year>2013</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2012</Year><Month>12</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1878-1667</ISSN><JournalIssue CitedMedium="Internet"><Volume>36</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Comparative immunology, microbiology and infectious diseases</Title><ISOAbbreviation>Comp. Immunol. Microbiol. Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi.</ArticleTitle><Pagination><MedlinePgn>25-38</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cimid.2012.09.004</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0147-9571(12)00104-X</ELocationID><Abstract><AbstractText>Wolbachia, the intracellular alpha-proteobacteria are required for the development, fertility and survival of filarial parasites. Wolbachia Translation initiation factor-1 (Wol Tl IF-1) is one of the factors required for Wolbachia growth and viability. In the present study, we cloned, over expressed and purified Wol Tl IF-1 that exhibited strong immuno-reactivity with various categories of bancroftian sera. Immunization with the recombinant protein resulted into significant reduction in microfilarial density (70-72%) and adult worm establishment (61-63%) in susceptible Mastomys coucha. Protection offered by Wol Tl IF-1 was found associated with humoral immune arm as observed by an increased antibody level with preponderance of IgE, IgM, IgG1 and IgG2a isotypes. The anti-Wol Tl IF-1 antibodies promoted profound adherence of peritoneal exudates cells to the surface of microfilariae and infective larvae causing cytotoxicity and their death. The present study indicates potential of recombinant Wol Tl IF-1 as a promising vaccine candidate against human lymphatic filarial infection.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nag</LastName><ForeName>Jeetendra Kumar</ForeName><Initials>JK</Initials><AffiliationInfo><Affiliation>Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shrivastava</LastName><ForeName>Nidhi</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Jyoti</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Misra-Bhattacharya</LastName><ForeName>Shailja</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate 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UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>37341-29-0</RegistryNumber><NameOfSubstance UI="D007073">Immunoglobulin E</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001402" MajorTopicYN="N">B-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002448" MajorTopicYN="N">Cell Adhesion</DescriptorName><QualifierName UI="Q000276" 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MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008213" MajorTopicYN="N">Lymphocyte Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008262" MajorTopicYN="N">Macrophage Activation</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008264" MajorTopicYN="N">Macrophages</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051189" MajorTopicYN="N">Murinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D039861" MajorTopicYN="N">Prokaryotic Initiation Factor-1</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000557" MajorTopicYN="N">secretion</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018418" MajorTopicYN="N">Th2 Cells</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020577" MajorTopicYN="N">Wolbachia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>08</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>09</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23079772</ArticleId><ArticleId IdType="pii">S0147-9571(12)00104-X</ArticleId><ArticleId IdType="doi">10.1016/j.cimid.2012.09.004</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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