Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope.
Identifieur interne : 000F11 ( PubMed/Corpus ); précédent : 000F10; suivant : 000F12Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope.
Auteurs : Michael V. D'Ambrosio ; Matthew Bakalar ; Sasisekhar Bennuru ; Clay Reber ; Arunan Skandarajah ; Lina Nilsson ; Neil Switz ; Joseph Kamgno ; Sébastien Pion ; Michel Boussinesq ; Thomas B. Nutman ; Daniel A. FletcherSource :
- Science translational medicine [ 1946-6242 ] ; 2015.
English descriptors
- KwdEn :
- Africa, Central, Algorithms, Animals, Automation, Cameroon, Cell Phones, Elephantiasis, Filarial (diagnosis), False Negative Reactions, Image Processing, Computer-Assisted, Loa (isolation & purification), Microscopy, Video (methods), Motion, Onchocerciasis (diagnosis), Point-of-Care Systems, Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- geographic : Africa, Central, Cameroon.
- diagnosis : Elephantiasis, Filarial, Onchocerciasis.
- isolation & purification : Loa.
- methods : Microscopy, Video.
- Algorithms, Animals, Automation, Cell Phones, False Negative Reactions, Image Processing, Computer-Assisted, Motion, Point-of-Care Systems, Reproducibility of Results, Sensitivity and Specificity.
Abstract
Parasitic helminths cause debilitating diseases that affect millions of people in primarily low-resource settings. Efforts to eliminate onchocerciasis and lymphatic filariasis in Central Africa through mass drug administration have been suspended because of ivermectin-associated serious adverse events, including death, in patients infected with the filarial parasite Loa loa. To safely administer ivermectin for onchocerciasis or lymphatic filariasis in regions co-endemic with L. loa, a strategy termed "test and (not) treat" has been proposed whereby those with high levels of L. loa microfilariae (>30,000/ml) that put them at risk for life-threatening serious adverse events are identified and excluded from mass drug administration. To enable this, we developed a mobile phone-based video microscope that automatically quantifies L. loa microfilariae in whole blood loaded directly into a small glass capillary from a fingerprick without the need for conventional sample preparation or staining. This point-of-care device automatically captures and analyzes videos of microfilarial motion in whole blood using motorized sample scanning and onboard motion detection, minimizing input from health care workers and providing a quantification of microfilariae per milliliter of whole blood in under 2 min. To validate performance and usability of the mobile phone microscope, we tested 33 potentially Loa-infected patients in Cameroon and confirmed that automated counts correlated with manual thick smear counts (94% specificity; 100% sensitivity). Use of this technology to exclude patients from ivermectin-based treatment at the point of care in Loa-endemic regions would allow resumption/expansion of mass drug administration programs for onchocerciasis and lymphatic filariasis in Central Africa.
DOI: 10.1126/scitranslmed.aaa3480
PubMed: 25947164
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D'Ambrosio</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bakalar, Matthew" sort="Bakalar, Matthew" uniqKey="Bakalar M" first="Matthew" last="Bakalar">Matthew Bakalar</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bennuru, Sasisekhar" sort="Bennuru, Sasisekhar" uniqKey="Bennuru S" first="Sasisekhar" last="Bennuru">Sasisekhar Bennuru</name><affiliation><nlm:affiliation>Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Reber, Clay" sort="Reber, Clay" uniqKey="Reber C" first="Clay" last="Reber">Clay Reber</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Skandarajah, Arunan" sort="Skandarajah, Arunan" uniqKey="Skandarajah A" first="Arunan" last="Skandarajah">Arunan Skandarajah</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Nilsson, Lina" sort="Nilsson, Lina" uniqKey="Nilsson L" first="Lina" last="Nilsson">Lina Nilsson</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Switz, Neil" sort="Switz, Neil" uniqKey="Switz N" first="Neil" last="Switz">Neil Switz</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA. 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UMI 233, Institut de Recherche pour le Développement and University of Montpellier, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Boussinesq, Michel" sort="Boussinesq, Michel" uniqKey="Boussinesq M" first="Michel" last="Boussinesq">Michel Boussinesq</name><affiliation><nlm:affiliation>UMI 233, Institut de Recherche pour le Développement and University of Montpellier, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Nutman, Thomas B" sort="Nutman, Thomas B" uniqKey="Nutman T" first="Thomas B" last="Nutman">Thomas B. Nutman</name><affiliation><nlm:affiliation>Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA. fletch@berkeley.edu tnutman@niaid.nih.gov.</nlm:affiliation></affiliation></author><author><name sortKey="Fletcher, Daniel A" sort="Fletcher, Daniel A" uniqKey="Fletcher D" first="Daniel A" last="Fletcher">Daniel A. 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D'Ambrosio</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bakalar, Matthew" sort="Bakalar, Matthew" uniqKey="Bakalar M" first="Matthew" last="Bakalar">Matthew Bakalar</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bennuru, Sasisekhar" sort="Bennuru, Sasisekhar" uniqKey="Bennuru S" first="Sasisekhar" last="Bennuru">Sasisekhar Bennuru</name><affiliation><nlm:affiliation>Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Reber, Clay" sort="Reber, Clay" uniqKey="Reber C" first="Clay" last="Reber">Clay Reber</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Skandarajah, Arunan" sort="Skandarajah, Arunan" uniqKey="Skandarajah A" first="Arunan" last="Skandarajah">Arunan Skandarajah</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Nilsson, Lina" sort="Nilsson, Lina" uniqKey="Nilsson L" first="Lina" last="Nilsson">Lina Nilsson</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Switz, Neil" sort="Switz, Neil" uniqKey="Switz N" first="Neil" last="Switz">Neil Switz</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA. Biophysics Graduate Group, UC Berkeley, Berkeley, CA 94720, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kamgno, Joseph" sort="Kamgno, Joseph" uniqKey="Kamgno J" first="Joseph" last="Kamgno">Joseph Kamgno</name><affiliation><nlm:affiliation>Center for Research on Filariasis and other Tropical Diseases, Yaoundé, Cameroon. Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.</nlm:affiliation></affiliation></author><author><name sortKey="Pion, Sebastien" sort="Pion, Sebastien" uniqKey="Pion S" first="Sébastien" last="Pion">Sébastien Pion</name><affiliation><nlm:affiliation>Center for Research on Filariasis and other Tropical Diseases, Yaoundé, Cameroon. UMI 233, Institut de Recherche pour le Développement and University of Montpellier, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Boussinesq, Michel" sort="Boussinesq, Michel" uniqKey="Boussinesq M" first="Michel" last="Boussinesq">Michel Boussinesq</name><affiliation><nlm:affiliation>UMI 233, Institut de Recherche pour le Développement and University of Montpellier, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Nutman, Thomas B" sort="Nutman, Thomas B" uniqKey="Nutman T" first="Thomas B" last="Nutman">Thomas B. Nutman</name><affiliation><nlm:affiliation>Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA. fletch@berkeley.edu tnutman@niaid.nih.gov.</nlm:affiliation></affiliation></author><author><name sortKey="Fletcher, Daniel A" sort="Fletcher, Daniel A" uniqKey="Fletcher D" first="Daniel A" last="Fletcher">Daniel A. Fletcher</name><affiliation><nlm:affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA. 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Efforts to eliminate onchocerciasis and lymphatic filariasis in Central Africa through mass drug administration have been suspended because of ivermectin-associated serious adverse events, including death, in patients infected with the filarial parasite Loa loa. To safely administer ivermectin for onchocerciasis or lymphatic filariasis in regions co-endemic with L. loa, a strategy termed "test and (not) treat" has been proposed whereby those with high levels of L. loa microfilariae (>30,000/ml) that put them at risk for life-threatening serious adverse events are identified and excluded from mass drug administration. To enable this, we developed a mobile phone-based video microscope that automatically quantifies L. loa microfilariae in whole blood loaded directly into a small glass capillary from a fingerprick without the need for conventional sample preparation or staining. This point-of-care device automatically captures and analyzes videos of microfilarial motion in whole blood using motorized sample scanning and onboard motion detection, minimizing input from health care workers and providing a quantification of microfilariae per milliliter of whole blood in under 2 min. To validate performance and usability of the mobile phone microscope, we tested 33 potentially Loa-infected patients in Cameroon and confirmed that automated counts correlated with manual thick smear counts (94% specificity; 100% sensitivity). Use of this technology to exclude patients from ivermectin-based treatment at the point of care in Loa-endemic regions would allow resumption/expansion of mass drug administration programs for onchocerciasis and lymphatic filariasis in Central Africa.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25947164</PMID><DateCreated><Year>2015</Year><Month>05</Month><Day>07</Day></DateCreated><DateCompleted><Year>2016</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2015</Year><Month>05</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1946-6242</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>286</Issue><PubDate><Year>2015</Year><Month>May</Month><Day>06</Day></PubDate></JournalIssue><Title>Science translational medicine</Title><ISOAbbreviation>Sci Transl Med</ISOAbbreviation></Journal><ArticleTitle>Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope.</ArticleTitle><Pagination><MedlinePgn>286re4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1126/scitranslmed.aaa3480</ELocationID><Abstract><AbstractText>Parasitic helminths cause debilitating diseases that affect millions of people in primarily low-resource settings. Efforts to eliminate onchocerciasis and lymphatic filariasis in Central Africa through mass drug administration have been suspended because of ivermectin-associated serious adverse events, including death, in patients infected with the filarial parasite Loa loa. To safely administer ivermectin for onchocerciasis or lymphatic filariasis in regions co-endemic with L. loa, a strategy termed "test and (not) treat" has been proposed whereby those with high levels of L. loa microfilariae (>30,000/ml) that put them at risk for life-threatening serious adverse events are identified and excluded from mass drug administration. To enable this, we developed a mobile phone-based video microscope that automatically quantifies L. loa microfilariae in whole blood loaded directly into a small glass capillary from a fingerprick without the need for conventional sample preparation or staining. This point-of-care device automatically captures and analyzes videos of microfilarial motion in whole blood using motorized sample scanning and onboard motion detection, minimizing input from health care workers and providing a quantification of microfilariae per milliliter of whole blood in under 2 min. To validate performance and usability of the mobile phone microscope, we tested 33 potentially Loa-infected patients in Cameroon and confirmed that automated counts correlated with manual thick smear counts (94% specificity; 100% sensitivity). Use of this technology to exclude patients from ivermectin-based treatment at the point of care in Loa-endemic regions would allow resumption/expansion of mass drug administration programs for onchocerciasis and lymphatic filariasis in Central Africa.</AbstractText><CopyrightInformation>Copyright © 2015, American Association for the Advancement of Science.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>D'Ambrosio</LastName><ForeName>Michael V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bakalar</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bennuru</LastName><ForeName>Sasisekhar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reber</LastName><ForeName>Clay</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Skandarajah</LastName><ForeName>Arunan</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nilsson</LastName><ForeName>Lina</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Switz</LastName><ForeName>Neil</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA. Biophysics Graduate Group, UC Berkeley, Berkeley, CA 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kamgno</LastName><ForeName>Joseph</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Center for Research on Filariasis and other Tropical Diseases, Yaoundé, Cameroon. Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pion</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Center for Research on Filariasis and other Tropical Diseases, Yaoundé, Cameroon. UMI 233, Institut de Recherche pour le Développement and University of Montpellier, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boussinesq</LastName><ForeName>Michel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>UMI 233, Institut de Recherche pour le Développement and University of Montpellier, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nutman</LastName><ForeName>Thomas B</ForeName><Initials>TB</Initials><AffiliationInfo><Affiliation>Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA. fletch@berkeley.edu tnutman@niaid.nih.gov.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fletcher</LastName><ForeName>Daniel A</ForeName><Initials>DA</Initials><AffiliationInfo><Affiliation>Department of Bioengineering, University of California (UC), Berkeley, Berkeley, CA 94720, USA. Biophysics Graduate Group, UC Berkeley, Berkeley, CA 94720, USA. fletch@berkeley.edu tnutman@niaid.nih.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Sci Transl Med</MedlineTA><NlmUniqueID>101505086</NlmUniqueID><ISSNLinking>1946-6234</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000350" MajorTopicYN="N" Type="Geographic">Africa, Central</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000465" MajorTopicYN="N">Algorithms</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001331" MajorTopicYN="N">Automation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002163" MajorTopicYN="N" Type="Geographic">Cameroon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040421" MajorTopicYN="Y">Cell Phones</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005188" MajorTopicYN="N">False Negative Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007091" MajorTopicYN="N">Image Processing, Computer-Assisted</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008117" MajorTopicYN="N">Loa</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018715" MajorTopicYN="N">Microscopy, Video</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009038" MajorTopicYN="N">Motion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009855" MajorTopicYN="N">Onchocerciasis</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019095" MajorTopicYN="Y">Point-of-Care Systems</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25947164</ArticleId><ArticleId IdType="pii">7/286/286re4</ArticleId><ArticleId IdType="doi">10.1126/scitranslmed.aaa3480</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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