Proteomic profiling of lymphedema development in mouse model.
Identifieur interne : 000847 ( PubMed/Corpus ); précédent : 000846; suivant : 000848Proteomic profiling of lymphedema development in mouse model.
Auteurs : Joomin Lee ; Haeun Song ; Kangsan Roh ; Sungrae Cho ; Sukchan Lee ; Chang-Hwan Yeom ; Seyeon ParkSource :
- Cell biochemistry and function [ 1099-0844 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Proteome.
- metabolism : Lymphedema.
- methods : Proteomics.
- pathology : Hindlimb, Lymphedema.
- Animals, Blotting, Western, Disease Models, Animal, Electrophoresis, Gel, Two-Dimensional, Male, Mice, Inbred ICR, Reproducibility of Results.
Abstract
The lymphatic vascular system plays an important role in tissue fluid homeostasis. Lymphedema is a chronic, progressive, and incurable condition that leads to lymphatic fluid retention; it may be primary (heritable) or secondary (acquired) in nature. Although there is a growing understanding of lymphedema, methods for the prevention and treatment of lymphedema are still limited. In this study, we investigated differential protein expressions in sham-operated and lymphedema-operated mice for 3 days, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced-right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A-1 preprotein, alpha-actinin-3, mCG21744, parkinson disease, serum amyloid P-component precursor, annexin A8, mKIAA0098 protein, and fibrinogen beta chain precursor were differentially upregulated in the lymphedema mice compared with the sham-operated group. Western blotting analysis was used to validate the proteomics results. Our results showing differential up-regulation of serum amyloid P-component precursor, parkinson disease, and apolipoprotein A-1 preprotein in lymphedema model over sham-operated model suggest important insights into pathophysiological target for lymphedema. Copyright © 2016 John Wiley & Sons, Ltd.
DOI: 10.1002/cbf.3192
PubMed: 27151289
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteomic profiling of lymphedema development in mouse model.</title><author><name sortKey="Lee, Joomin" sort="Lee, Joomin" uniqKey="Lee J" first="Joomin" last="Lee">Joomin Lee</name><affiliation><nlm:affiliation>Department of food and nutrition, College of Natural Science, Chosun University, Gwangju, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Haeun" sort="Song, Haeun" uniqKey="Song H" first="Haeun" last="Song">Haeun Song</name><affiliation><nlm:affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Roh, Kangsan" sort="Roh, Kangsan" uniqKey="Roh K" first="Kangsan" last="Roh">Kangsan Roh</name><affiliation><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Sungrae" sort="Cho, Sungrae" uniqKey="Cho S" first="Sungrae" last="Cho">Sungrae Cho</name><affiliation><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sukchan" sort="Lee, Sukchan" uniqKey="Lee S" first="Sukchan" last="Lee">Sukchan Lee</name><affiliation><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Yeom, Chang Hwan" sort="Yeom, Chang Hwan" uniqKey="Yeom C" first="Chang-Hwan" last="Yeom">Chang-Hwan Yeom</name><affiliation><nlm:affiliation>Yeom's Clinic of Palliative Medicine, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Park, Seyeon" sort="Park, Seyeon" uniqKey="Park S" first="Seyeon" last="Park">Seyeon Park</name><affiliation><nlm:affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27151289</idno><idno type="pmid">27151289</idno><idno type="doi">10.1002/cbf.3192</idno><idno type="wicri:Area/PubMed/Corpus">000847</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000847</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomic profiling of lymphedema development in mouse model.</title><author><name sortKey="Lee, Joomin" sort="Lee, Joomin" uniqKey="Lee J" first="Joomin" last="Lee">Joomin Lee</name><affiliation><nlm:affiliation>Department of food and nutrition, College of Natural Science, Chosun University, Gwangju, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Haeun" sort="Song, Haeun" uniqKey="Song H" first="Haeun" last="Song">Haeun Song</name><affiliation><nlm:affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Roh, Kangsan" sort="Roh, Kangsan" uniqKey="Roh K" first="Kangsan" last="Roh">Kangsan Roh</name><affiliation><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Sungrae" sort="Cho, Sungrae" uniqKey="Cho S" first="Sungrae" last="Cho">Sungrae Cho</name><affiliation><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sukchan" sort="Lee, Sukchan" uniqKey="Lee S" first="Sukchan" last="Lee">Sukchan Lee</name><affiliation><nlm:affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Yeom, Chang Hwan" sort="Yeom, Chang Hwan" uniqKey="Yeom C" first="Chang-Hwan" last="Yeom">Chang-Hwan Yeom</name><affiliation><nlm:affiliation>Yeom's Clinic of Palliative Medicine, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Park, Seyeon" sort="Park, Seyeon" uniqKey="Park S" first="Seyeon" last="Park">Seyeon Park</name><affiliation><nlm:affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Cell biochemistry and function</title><idno type="eISSN">1099-0844</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Disease Models, Animal</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Hindlimb (pathology)</term><term>Lymphedema (metabolism)</term><term>Lymphedema (pathology)</term><term>Male</term><term>Mice, Inbred ICR</term><term>Proteome (metabolism)</term><term>Proteomics (methods)</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Hindlimb</term><term>Lymphedema</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Disease Models, Animal</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Male</term><term>Mice, Inbred ICR</term><term>Reproducibility of Results</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The lymphatic vascular system plays an important role in tissue fluid homeostasis. Lymphedema is a chronic, progressive, and incurable condition that leads to lymphatic fluid retention; it may be primary (heritable) or secondary (acquired) in nature. Although there is a growing understanding of lymphedema, methods for the prevention and treatment of lymphedema are still limited. In this study, we investigated differential protein expressions in sham-operated and lymphedema-operated mice for 3 days, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced-right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A-1 preprotein, alpha-actinin-3, mCG21744, parkinson disease, serum amyloid P-component precursor, annexin A8, mKIAA0098 protein, and fibrinogen beta chain precursor were differentially upregulated in the lymphedema mice compared with the sham-operated group. Western blotting analysis was used to validate the proteomics results. Our results showing differential up-regulation of serum amyloid P-component precursor, parkinson disease, and apolipoprotein A-1 preprotein in lymphedema model over sham-operated model suggest important insights into pathophysiological target for lymphedema. Copyright © 2016 John Wiley & Sons, Ltd.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27151289</PMID><DateCreated><Year>2016</Year><Month>06</Month><Day>24</Day></DateCreated><DateCompleted><Year>2017</Year><Month>01</Month><Day>23</Day></DateCompleted><DateRevised><Year>2017</Year><Month>01</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1099-0844</ISSN><JournalIssue CitedMedium="Internet"><Volume>34</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Cell biochemistry and function</Title><ISOAbbreviation>Cell Biochem. Funct.</ISOAbbreviation></Journal><ArticleTitle>Proteomic profiling of lymphedema development in mouse model.</ArticleTitle><Pagination><MedlinePgn>317-25</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/cbf.3192</ELocationID><Abstract><AbstractText>The lymphatic vascular system plays an important role in tissue fluid homeostasis. Lymphedema is a chronic, progressive, and incurable condition that leads to lymphatic fluid retention; it may be primary (heritable) or secondary (acquired) in nature. Although there is a growing understanding of lymphedema, methods for the prevention and treatment of lymphedema are still limited. In this study, we investigated differential protein expressions in sham-operated and lymphedema-operated mice for 3 days, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced-right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A-1 preprotein, alpha-actinin-3, mCG21744, parkinson disease, serum amyloid P-component precursor, annexin A8, mKIAA0098 protein, and fibrinogen beta chain precursor were differentially upregulated in the lymphedema mice compared with the sham-operated group. Western blotting analysis was used to validate the proteomics results. Our results showing differential up-regulation of serum amyloid P-component precursor, parkinson disease, and apolipoprotein A-1 preprotein in lymphedema model over sham-operated model suggest important insights into pathophysiological target for lymphedema. Copyright © 2016 John Wiley & Sons, Ltd.</AbstractText><CopyrightInformation>Copyright © 2016 John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Joomin</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of food and nutrition, College of Natural Science, Chosun University, Gwangju, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Haeun</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Yeom's Clinic of Palliative Medicine, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roh</LastName><ForeName>Kangsan</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Sungrae</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sukchan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yeom</LastName><ForeName>Chang-Hwan</ForeName><Initials>CH</Initials><AffiliationInfo><Affiliation>Yeom's Clinic of Palliative Medicine, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Seyeon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Applied Chemistry, Dongduk Women's University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cell Biochem Funct</MedlineTA><NlmUniqueID>8305874</NlmUniqueID><ISSNLinking>0263-6484</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006614" MajorTopicYN="N">Hindlimb</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008209" MajorTopicYN="N">Lymphedema</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008813" MajorTopicYN="N">Mice, Inbred ICR</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">development</Keyword><Keyword MajorTopicYN="N">lymphedema</Keyword><Keyword MajorTopicYN="N">mouse model</Keyword><Keyword MajorTopicYN="N">proteomics</Keyword><Keyword MajorTopicYN="N">serum amyloid P-component precursor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>11</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>04</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>18</Day></PubMedPubDate><PubMedPubDate 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