Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.
Identifieur interne : 000400 ( PubMed/Corpus ); précédent : 000399; suivant : 000401Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.
Auteurs : Iman R. Abdel-Shafi ; Eman Y. Shoieb ; Samar S. Attia ; José M. Rubio ; Thuy-Huong Ta-Tang ; Ayman A. El-BadrySource :
- Parasitology research [ 1432-1955 ] ; 2017.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Base Sequence, Blood (parasitology), Child, Child, Preschool, Culex (genetics), Egypt (epidemiology), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (parasitology), Female, Humans, Infant, Male, Middle Aged, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Wuchereria bancrofti (classification), Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification), Young Adult.
- MESH :
- blood : Elephantiasis, Filarial.
- classification : Wuchereria bancrofti.
- epidemiology : Egypt, Elephantiasis, Filarial.
- genetics : Culex, Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- parasitology : Blood, Elephantiasis, Filarial.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Base Sequence, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Young Adult.
Abstract
Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.
DOI: 10.1007/s00436-017-5372-7
PubMed: 28120100
Links to Exploration step
pubmed:28120100Le document en format XML
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Attia</name><affiliation><nlm:affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</nlm:affiliation></affiliation></author><author><name sortKey="Rubio, Jose M" sort="Rubio, Jose M" uniqKey="Rubio J" first="José M" last="Rubio">José M. Rubio</name><affiliation><nlm:affiliation>Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Ta Tang, Thuy Huong" sort="Ta Tang, Thuy Huong" uniqKey="Ta Tang T" first="Thuy-Huong" last="Ta-Tang">Thuy-Huong Ta-Tang</name><affiliation><nlm:affiliation>Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="El Badry, Ayman A" sort="El Badry, Ayman A" uniqKey="El Badry A" first="Ayman A" last="El-Badry">Ayman A. 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Abdel-Shafi</name><affiliation><nlm:affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</nlm:affiliation></affiliation></author><author><name sortKey="Shoieb, Eman Y" sort="Shoieb, Eman Y" uniqKey="Shoieb E" first="Eman Y" last="Shoieb">Eman Y. Shoieb</name><affiliation><nlm:affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</nlm:affiliation></affiliation></author><author><name sortKey="Attia, Samar S" sort="Attia, Samar S" uniqKey="Attia S" first="Samar S" last="Attia">Samar S. Attia</name><affiliation><nlm:affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</nlm:affiliation></affiliation></author><author><name sortKey="Rubio, Jose M" sort="Rubio, Jose M" uniqKey="Rubio J" first="José M" last="Rubio">José M. Rubio</name><affiliation><nlm:affiliation>Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Ta Tang, Thuy Huong" sort="Ta Tang, Thuy Huong" uniqKey="Ta Tang T" first="Thuy-Huong" last="Ta-Tang">Thuy-Huong Ta-Tang</name><affiliation><nlm:affiliation>Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="El Badry, Ayman A" sort="El Badry, Ayman A" uniqKey="El Badry A" first="Ayman A" last="El-Badry">Ayman A. El-Badry</name><affiliation><nlm:affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt. aelbadry@kasralainy.edu.eg.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Parasitology research</title><idno type="eISSN">1432-1955</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Base Sequence</term><term>Blood (parasitology)</term><term>Child</term><term>Child, Preschool</term><term>Culex (genetics)</term><term>Egypt (epidemiology)</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (epidemiology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Wuchereria bancrofti (classification)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term><term>Young Adult</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Egypt</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Culex</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Blood</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Base Sequence</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Sequence Alignment</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28120100</PMID><DateCreated><Year>2017</Year><Month>01</Month><Day>25</Day></DateCreated><DateCompleted><Year>2017</Year><Month>06</Month><Day>28</Day></DateCompleted><DateRevised><Year>2017</Year><Month>06</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1955</ISSN><JournalIssue CitedMedium="Internet"><Volume>116</Volume><Issue>3</Issue><PubDate><Year>2017</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Parasitology research</Title><ISOAbbreviation>Parasitol. Res.</ISOAbbreviation></Journal><ArticleTitle>Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.</ArticleTitle><Pagination><MedlinePgn>963-970</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00436-017-5372-7</ELocationID><Abstract><AbstractText>Lymphatic filariasis (LF) is a serious vector-borne health problem, and Wuchereria bancrofti (W.b) is the major cause of LF worldwide and is focally endemic in Egypt. Identification of filarial infection using traditional morphologic and immunological criteria can be difficult and lead to misdiagnosis. The aim of the present study was molecular detection of W.b in residents in endemic areas in Egypt, sequence variance analysis, and phylogenetic analysis of W.b DNA. Collected blood samples from residents in filariasis endemic areas in five governorates were subjected to semi-nested PCR targeting repeated DNA sequence, for detection of W.b DNA. PCR products were sequenced; subsequently, a phylogenetic analysis of the obtained sequences was performed. Out of 300 blood samples, W.b DNA was identified in 48 (16%). Sequencing analysis confirmed PCR results identifying only W.b species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W.b among the study population. Study results demonstrated that the semi-nested PCR proved to be an effective diagnostic tool for accurate and rapid detection of W.b infections in nano-epidemics and is applicable for samples collected in the daytime as well as the night time. PCR products sequencing and phylogenitic analysis revealed three different nucleotide sequences variants. Further genetic studies of W.b in Egypt and other endemic areas are needed to distinguish related strains and the various ecological as well as drug effects exerted on them to support W.b elimination.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abdel-Shafi</LastName><ForeName>Iman R</ForeName><Initials>IR</Initials><AffiliationInfo><Affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shoieb</LastName><ForeName>Eman Y</ForeName><Initials>EY</Initials><AffiliationInfo><Affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Attia</LastName><ForeName>Samar S</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rubio</LastName><ForeName>José M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ta-Tang</LastName><ForeName>Thuy-Huong</ForeName><Initials>TH</Initials><AffiliationInfo><Affiliation>Malaria & Emerging Parasitic Diseases Laboratory (MAPELab), National Microbiology Center, Instituto De Salud Carlos III, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>El-Badry</LastName><ForeName>Ayman A</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt. aelbadry@kasralainy.edu.eg.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Parasitol Res</MedlineTA><NlmUniqueID>8703571</NlmUniqueID><ISSNLinking>0932-0113</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001769" MajorTopicYN="N">Blood</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003465" MajorTopicYN="N">Culex</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004534" MajorTopicYN="N">Egypt</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phylogenetic analysis</Keyword><Keyword MajorTopicYN="N">Repeated DNA sequence</Keyword><Keyword MajorTopicYN="N">Semi-nested PCR</Keyword><Keyword MajorTopicYN="N">Wuchereria bancrofti</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>6</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28120100</ArticleId><ArticleId IdType="doi">10.1007/s00436-017-5372-7</ArticleId><ArticleId IdType="pii">10.1007/s00436-017-5372-7</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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