Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.
Identifieur interne : 000214 ( PubMed/Corpus ); précédent : 000213; suivant : 000215Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.
Auteurs : Sheri Wang ; Daibang Nie ; J Peter Rubin ; Lauren KokaiSource :
- Lymphatic research and biology [ 1557-8585 ] ; 2017.
Abstract
Secondary lymphedema, resulting from damage to lymphatic vessels, is a common sequela following surgical removal of lymph nodes for cancer. Current therapeutics for treating lymphedema are limited and further research on underlying causes is warranted. Published studies on molecular mechanisms of lymphedema primarily focus on lymphatic endothelial cells (LECs), which comprise the innermost lining of lymphatic capillaries and collecting vessels. However, traditional static culture of LECs may not adequately recapitulate the lymphedemous cell phenotype as transcriptomal comparison of human dermal LECs has shown significant differences in ex vivo and in vitro LEC gene expression. In this study, we designed a dynamic culture system, in which LECs were exposed to physiologic and excess mechanical strain to determine if native and lymphedemous phenotypes could be reproduced in vitro.
DOI: 10.1089/lrb.2016.0042
PubMed: 28486010
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.</title><author><name sortKey="Wang, Sheri" sort="Wang, Sheri" uniqKey="Wang S" first="Sheri" last="Wang">Sheri Wang</name><affiliation><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author><author><name sortKey="Nie, Daibang" sort="Nie, Daibang" uniqKey="Nie D" first="Daibang" last="Nie">Daibang Nie</name><affiliation><nlm:affiliation>2 Department of Orthopaedic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author><author><name sortKey="Rubin, J Peter" sort="Rubin, J Peter" uniqKey="Rubin J" first="J Peter" last="Rubin">J Peter Rubin</name><affiliation><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author><author><name sortKey="Kokai, Lauren" sort="Kokai, Lauren" uniqKey="Kokai L" first="Lauren" last="Kokai">Lauren Kokai</name><affiliation><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28486010</idno><idno type="pmid">28486010</idno><idno type="doi">10.1089/lrb.2016.0042</idno><idno type="wicri:Area/PubMed/Corpus">000214</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000214</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.</title><author><name sortKey="Wang, Sheri" sort="Wang, Sheri" uniqKey="Wang S" first="Sheri" last="Wang">Sheri Wang</name><affiliation><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author><author><name sortKey="Nie, Daibang" sort="Nie, Daibang" uniqKey="Nie D" first="Daibang" last="Nie">Daibang Nie</name><affiliation><nlm:affiliation>2 Department of Orthopaedic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author><author><name sortKey="Rubin, J Peter" sort="Rubin, J Peter" uniqKey="Rubin J" first="J Peter" last="Rubin">J Peter Rubin</name><affiliation><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author><author><name sortKey="Kokai, Lauren" sort="Kokai, Lauren" uniqKey="Kokai L" first="Lauren" last="Kokai">Lauren Kokai</name><affiliation><nlm:affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Lymphatic research and biology</title><idno type="eISSN">1557-8585</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Secondary lymphedema, resulting from damage to lymphatic vessels, is a common sequela following surgical removal of lymph nodes for cancer. Current therapeutics for treating lymphedema are limited and further research on underlying causes is warranted. Published studies on molecular mechanisms of lymphedema primarily focus on lymphatic endothelial cells (LECs), which comprise the innermost lining of lymphatic capillaries and collecting vessels. However, traditional static culture of LECs may not adequately recapitulate the lymphedemous cell phenotype as transcriptomal comparison of human dermal LECs has shown significant differences in ex vivo and in vitro LEC gene expression. In this study, we designed a dynamic culture system, in which LECs were exposed to physiologic and excess mechanical strain to determine if native and lymphedemous phenotypes could be reproduced in vitro.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">28486010</PMID><DateCreated><Year>2017</Year><Month>05</Month><Day>09</Day></DateCreated><DateRevised><Year>2017</Year><Month>06</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1557-8585</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>2</Issue><PubDate><Year>2017</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Lymphatic research and biology</Title><ISOAbbreviation>Lymphat Res Biol</ISOAbbreviation></Journal><ArticleTitle>Lymphatic Endothelial Cells under Mechanical Stress: Altered Expression of Inflammatory Cytokines and Fibrosis.</ArticleTitle><Pagination><MedlinePgn>130-135</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1089/lrb.2016.0042</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Secondary lymphedema, resulting from damage to lymphatic vessels, is a common sequela following surgical removal of lymph nodes for cancer. Current therapeutics for treating lymphedema are limited and further research on underlying causes is warranted. Published studies on molecular mechanisms of lymphedema primarily focus on lymphatic endothelial cells (LECs), which comprise the innermost lining of lymphatic capillaries and collecting vessels. However, traditional static culture of LECs may not adequately recapitulate the lymphedemous cell phenotype as transcriptomal comparison of human dermal LECs has shown significant differences in ex vivo and in vitro LEC gene expression. In this study, we designed a dynamic culture system, in which LECs were exposed to physiologic and excess mechanical strain to determine if native and lymphedemous phenotypes could be reproduced in vitro.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Purified human LECs were cultured in silicon dishes and subjected to 0% (control), 4%, and 8% mechanical strain for 72 hours. Our results indicate that control and stretched LECs maintained a mature phenotype. Extreme stretching at 8% strain significantly increased LEC proliferation and significantly increased Prox1 expression, suggesting a lymphedemous cell phenotype resulting with lymphangiogenesis.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Mechanical strain reinforced a mature lymphatic phenotype and excess strain promoted lymphangiogenesis, while altering collagen deposition and cytokine secretion.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Sheri</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nie</LastName><ForeName>Daibang</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>2 Department of Orthopaedic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rubin</LastName><ForeName>J Peter</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kokai</LastName><ForeName>Lauren</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>1 Department of Plastic Surgery, University of Pittsburgh , Pittsburgh, Pennsylvania.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Lymphat Res Biol</MedlineTA><NlmUniqueID>101163587</NlmUniqueID><ISSNLinking>1539-6851</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">lymphangiogenesis</Keyword><Keyword MajorTopicYN="N">lymphatic endothelial cells</Keyword><Keyword MajorTopicYN="N">lymphedema</Keyword><Keyword MajorTopicYN="N">mechanical strain</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28486010</ArticleId><ArticleId IdType="doi">10.1089/lrb.2016.0042</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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