Comparison and functional characterization of peripheral blood mononuclear cells isolated from filarial lymphedema and endemic normals of a South Indian population.
Identifieur interne : 000022 ( PubMed/Corpus ); précédent : 000021; suivant : 000023Comparison and functional characterization of peripheral blood mononuclear cells isolated from filarial lymphedema and endemic normals of a South Indian population.
Auteurs : Abel Arul Nathan ; Madhulika Dixit ; Subash Babu ; Anand Setty BalakrishnanSource :
- Tropical medicine & international health : TM & IH [ 1365-3156 ] ; 2017.
Abstract
The underlying problem in lymphatic filariasis is irreversible swelling of the limbs (lymphedema), which is a unique feature of lymphatic insufficiency. It is still unclear whether the natural ability of lymphatics to form functional lymphatic vasculature is achieved or attenuated in the lymphedemal pathology. Clinical studies have clearly shown that circulating lymphatic progenitors (CLPs), a subset of bone-marrow derived mononuclear cells (PBMCs), contribute to post-natal lymph vasculogenesis. CLP-based revascularization could be a promising strategy to bypass the endothelial disruption and damage incurred by the filarial parasites. Thus our aim was to compare and characterize the functional prowess of PBMCs in physiological and lymphedemal pathology.
DOI: 10.1111/tmi.12969
PubMed: 28869696
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison and functional characterization of peripheral blood mononuclear cells isolated from filarial lymphedema and endemic normals of a South Indian population.</title><author><name sortKey="Nathan, Abel Arul" sort="Nathan, Abel Arul" uniqKey="Nathan A" first="Abel Arul" last="Nathan">Abel Arul Nathan</name><affiliation><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.</nlm:affiliation></affiliation></author><author><name sortKey="Dixit, Madhulika" sort="Dixit, Madhulika" uniqKey="Dixit M" first="Madhulika" last="Dixit">Madhulika Dixit</name><affiliation><nlm:affiliation>Laboratory of Vascular Biology, Department of Biotechnology, Bhupat Joyti Metha School of Biosciences and Bioengineering, Indian Institute of Technology Madras, India.</nlm:affiliation></affiliation></author><author><name sortKey="Babu, Subash" sort="Babu, Subash" uniqKey="Babu S" first="Subash" last="Babu">Subash Babu</name><affiliation><nlm:affiliation>NIH-ICER, National Institute for Research in Tuberculosis, Chennai, India.</nlm:affiliation></affiliation></author><author><name sortKey="Balakrishnan, Anand Setty" sort="Balakrishnan, Anand Setty" uniqKey="Balakrishnan A" first="Anand Setty" last="Balakrishnan">Anand Setty Balakrishnan</name><affiliation><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28869696</idno><idno type="pmid">28869696</idno><idno type="doi">10.1111/tmi.12969</idno><idno type="wicri:Area/PubMed/Corpus">000022</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000022</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison and functional characterization of peripheral blood mononuclear cells isolated from filarial lymphedema and endemic normals of a South Indian population.</title><author><name sortKey="Nathan, Abel Arul" sort="Nathan, Abel Arul" uniqKey="Nathan A" first="Abel Arul" last="Nathan">Abel Arul Nathan</name><affiliation><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.</nlm:affiliation></affiliation></author><author><name sortKey="Dixit, Madhulika" sort="Dixit, Madhulika" uniqKey="Dixit M" first="Madhulika" last="Dixit">Madhulika Dixit</name><affiliation><nlm:affiliation>Laboratory of Vascular Biology, Department of Biotechnology, Bhupat Joyti Metha School of Biosciences and Bioengineering, Indian Institute of Technology Madras, India.</nlm:affiliation></affiliation></author><author><name sortKey="Babu, Subash" sort="Babu, Subash" uniqKey="Babu S" first="Subash" last="Babu">Subash Babu</name><affiliation><nlm:affiliation>NIH-ICER, National Institute for Research in Tuberculosis, Chennai, India.</nlm:affiliation></affiliation></author><author><name sortKey="Balakrishnan, Anand Setty" sort="Balakrishnan, Anand Setty" uniqKey="Balakrishnan A" first="Anand Setty" last="Balakrishnan">Anand Setty Balakrishnan</name><affiliation><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Tropical medicine & international health : TM & IH</title><idno type="eISSN">1365-3156</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The underlying problem in lymphatic filariasis is irreversible swelling of the limbs (lymphedema), which is a unique feature of lymphatic insufficiency. It is still unclear whether the natural ability of lymphatics to form functional lymphatic vasculature is achieved or attenuated in the lymphedemal pathology. Clinical studies have clearly shown that circulating lymphatic progenitors (CLPs), a subset of bone-marrow derived mononuclear cells (PBMCs), contribute to post-natal lymph vasculogenesis. CLP-based revascularization could be a promising strategy to bypass the endothelial disruption and damage incurred by the filarial parasites. Thus our aim was to compare and characterize the functional prowess of PBMCs in physiological and lymphedemal pathology.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">28869696</PMID><DateCreated><Year>2017</Year><Month>09</Month><Day>04</Day></DateCreated><DateRevised><Year>2017</Year><Month>09</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3156</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2017</Year><Month>Sep</Month><Day>04</Day></PubDate></JournalIssue><Title>Tropical medicine & international health : TM & IH</Title><ISOAbbreviation>Trop. Med. Int. Health</ISOAbbreviation></Journal><ArticleTitle>Comparison and functional characterization of peripheral blood mononuclear cells isolated from filarial lymphedema and endemic normals of a South Indian population.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/tmi.12969</ELocationID><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">The underlying problem in lymphatic filariasis is irreversible swelling of the limbs (lymphedema), which is a unique feature of lymphatic insufficiency. It is still unclear whether the natural ability of lymphatics to form functional lymphatic vasculature is achieved or attenuated in the lymphedemal pathology. Clinical studies have clearly shown that circulating lymphatic progenitors (CLPs), a subset of bone-marrow derived mononuclear cells (PBMCs), contribute to post-natal lymph vasculogenesis. CLP-based revascularization could be a promising strategy to bypass the endothelial disruption and damage incurred by the filarial parasites. Thus our aim was to compare and characterize the functional prowess of PBMCs in physiological and lymphedemal pathology.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">PBMCs were isolated from venous blood sample from drug-naive endemic normals (EN) and drug deprived filarial lymphedema (FL) individuals using density gradient centrifugation. Adhesion, Trans-well migration and in-vitro matrigel assays were employed to characterize the lymphvasculogenic potential of PBMCs. CLPs were phenotypically characterized using flow cytometry; expression levels of lymphatic markers and inflammatory cytokines were quantified using qRT-PCR and ELISA respectively.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">PBMCs from FL group display poor adherence to fibronectin (p=0.040), reduced migration towards SDF-1α (p=0.035), impaired tubular network (p=0.004) and branching point (p=0.048) formation. The PBMCs mRNA expression of VEGFR3 (p=0.039) and Podoplanin (p=0.050) were elevated, whereas Integrin α9 (p=0.046) was inhibited in FL individuals; additionally the surface expression of CD34 (p=0.048) was significantly reduced in the FL group compared to the EN group.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">PBMCs from filarial lymphedema show defective and dysregulated lymph vasculogenic function compared to endemic normals. This article is protected by copyright. All rights reserved.</AbstractText><CopyrightInformation>This article is protected by copyright. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nathan</LastName><ForeName>Abel Arul</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dixit</LastName><ForeName>Madhulika</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Vascular Biology, Department of Biotechnology, Bhupat Joyti Metha School of Biosciences and Bioengineering, Indian Institute of Technology Madras, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babu</LastName><ForeName>Subash</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>NIH-ICER, National Institute for Research in Tuberculosis, Chennai, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Balakrishnan</LastName><ForeName>Anand Setty</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>09</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Trop Med Int Health</MedlineTA><NlmUniqueID>9610576</NlmUniqueID><ISSNLinking>1360-2276</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Lymphangiogenesis</Keyword><Keyword MajorTopicYN="N">Lymphatic Endothelial progenitor cells</Keyword><Keyword MajorTopicYN="N">Lymphatic filariasis</Keyword><Keyword MajorTopicYN="N">Peripheral blood mononuclear cells</Keyword><Keyword MajorTopicYN="N">Secondary lymphedema</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>9</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28869696</ArticleId><ArticleId IdType="doi">10.1111/tmi.12969</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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