Cloning and characterization of a potentially protective antigen in lymphatic filariasis.
Identifieur interne : 005998 ( PubMed/Checkpoint ); précédent : 005997; suivant : 005999Cloning and characterization of a potentially protective antigen in lymphatic filariasis.
Auteurs : T W Nilsen [États-Unis] ; P A Maroney ; R G Goodwin ; K G Perrine ; J A Denker ; J. Nanduri ; J W KazuraSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1988.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ADN, Antigènes d'helminthe, Brugia.
- immunologie : Filariose lymphatique, Filarioses.
- Animaux, Clonage moléculaire, Données de séquences moléculaires, Gerbillinae, Gènes, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Antigens, Helminth, DNA.
- genetics : Brugia.
- immunology : Elephantiasis, Filarial, Filariasis.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Genes, Gerbillinae, Molecular Sequence Data.
Abstract
To facilitate biochemical studies of protective filarial antigens, a lambda gt11 cDNA library was constructed from Brugia malayi adult mRNA and screened with rabbit sera that recognizes a limited set of filarial antigens of approximately 25, 42, 60, and 112 kDa. Antigens of approximately equal to 25 and approximately equal to 60 kDa have been shown previously to induce enhanced clearance of microfilaremia in mice. A 154-base pair clone detected by immunological reactivity was used to isolate by hybridization a nearly full-length cDNA clone of 1.8 kilobases. Nucleotide-sequence analysis indicated that this clone was derived from a mRNA encoding a 63-kDa antigen. A fusion polypeptide containing 37 kDa of the Escherichia coli TrpE protein (anthranilate synthase) and 55 kDa of the cloned protein was recognized in immunoblot experiments with antisera raised against a partially purified preparation of the approximately equal to 60-kDa protective filarial antigen. These data relate the cloned antigen to a potentially protective antigen in lymphatic filariasis.
PubMed: 3368467
Affiliations:
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Nanduri</name></author><author><name sortKey="Kazura, J W" sort="Kazura, J W" uniqKey="Kazura J" first="J W" last="Kazura">J W Kazura</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1988">1988</date><idno type="RBID">pubmed:3368467</idno><idno type="pmid">3368467</idno><idno type="wicri:Area/PubMed/Corpus">006187</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">006187</idno><idno type="wicri:Area/PubMed/Curation">006187</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">006187</idno><idno type="wicri:Area/PubMed/Checkpoint">006187</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">006187</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning and characterization of a potentially protective antigen in lymphatic filariasis.</title><author><name sortKey="Nilsen, T W" sort="Nilsen, T W" uniqKey="Nilsen T" first="T W" last="Nilsen">T W Nilsen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine and University Hospitals, Cleveland, OH 44106.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine and University Hospitals, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Maroney, P A" sort="Maroney, P A" uniqKey="Maroney P" first="P A" last="Maroney">P A Maroney</name></author><author><name sortKey="Goodwin, R G" sort="Goodwin, R G" uniqKey="Goodwin R" first="R G" last="Goodwin">R G Goodwin</name></author><author><name sortKey="Perrine, K G" sort="Perrine, K G" uniqKey="Perrine K" first="K G" last="Perrine">K G Perrine</name></author><author><name sortKey="Denker, J A" sort="Denker, J A" uniqKey="Denker J" first="J A" last="Denker">J A Denker</name></author><author><name sortKey="Nanduri, J" sort="Nanduri, J" uniqKey="Nanduri J" first="J" last="Nanduri">J. Nanduri</name></author><author><name sortKey="Kazura, J W" sort="Kazura, J W" uniqKey="Kazura J" first="J W" last="Kazura">J W Kazura</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1988" type="published">1988</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antigens, Helminth (genetics)</term><term>Base Sequence</term><term>Brugia (genetics)</term><term>Cloning, Molecular</term><term>DNA (genetics)</term><term>Elephantiasis, Filarial (immunology)</term><term>Filariasis (immunology)</term><term>Genes</term><term>Gerbillinae</term><term>Molecular Sequence Data</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (génétique)</term><term>Animaux</term><term>Antigènes d'helminthe (génétique)</term><term>Brugia (génétique)</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique (immunologie)</term><term>Filarioses (immunologie)</term><term>Gerbillinae</term><term>Gènes</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>DNA</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>Antigènes d'helminthe</term><term>Brugia</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Filarioses</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Filariasis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Genes</term><term>Gerbillinae</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Gerbillinae</term><term>Gènes</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To facilitate biochemical studies of protective filarial antigens, a lambda gt11 cDNA library was constructed from Brugia malayi adult mRNA and screened with rabbit sera that recognizes a limited set of filarial antigens of approximately 25, 42, 60, and 112 kDa. Antigens of approximately equal to 25 and approximately equal to 60 kDa have been shown previously to induce enhanced clearance of microfilaremia in mice. A 154-base pair clone detected by immunological reactivity was used to isolate by hybridization a nearly full-length cDNA clone of 1.8 kilobases. Nucleotide-sequence analysis indicated that this clone was derived from a mRNA encoding a 63-kDa antigen. A fusion polypeptide containing 37 kDa of the Escherichia coli TrpE protein (anthranilate synthase) and 55 kDa of the cloned protein was recognized in immunoblot experiments with antisera raised against a partially purified preparation of the approximately equal to 60-kDa protective filarial antigen. These data relate the cloned antigen to a potentially protective antigen in lymphatic filariasis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3368467</PMID><DateCreated><Year>1988</Year><Month>06</Month><Day>22</Day></DateCreated><DateCompleted><Year>1988</Year><Month>06</Month><Day>22</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>85</Volume><Issue>10</Issue><PubDate><Year>1988</Year><Month>May</Month></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Cloning and characterization of a potentially protective antigen in lymphatic filariasis.</ArticleTitle><Pagination><MedlinePgn>3604-7</MedlinePgn></Pagination><Abstract><AbstractText>To facilitate biochemical studies of protective filarial antigens, a lambda gt11 cDNA library was constructed from Brugia malayi adult mRNA and screened with rabbit sera that recognizes a limited set of filarial antigens of approximately 25, 42, 60, and 112 kDa. Antigens of approximately equal to 25 and approximately equal to 60 kDa have been shown previously to induce enhanced clearance of microfilaremia in mice. A 154-base pair clone detected by immunological reactivity was used to isolate by hybridization a nearly full-length cDNA clone of 1.8 kilobases. Nucleotide-sequence analysis indicated that this clone was derived from a mRNA encoding a 63-kDa antigen. A fusion polypeptide containing 37 kDa of the Escherichia coli TrpE protein (anthranilate synthase) and 55 kDa of the cloned protein was recognized in immunoblot experiments with antisera raised against a partially purified preparation of the approximately equal to 60-kDa protective filarial antigen. These data relate the cloned antigen to a potentially protective antigen in lymphatic filariasis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nilsen</LastName><ForeName>T W</ForeName><Initials>TW</Initials><AffiliationInfo><Affiliation>Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine and University Hospitals, Cleveland, OH 44106.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maroney</LastName><ForeName>P A</ForeName><Initials>PA</Initials></Author><Author ValidYN="Y"><LastName>Goodwin</LastName><ForeName>R G</ForeName><Initials>RG</Initials></Author><Author ValidYN="Y"><LastName>Perrine</LastName><ForeName>K G</ForeName><Initials>KG</Initials></Author><Author ValidYN="Y"><LastName>Denker</LastName><ForeName>J A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Nanduri</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author 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P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Parasitol. 1970 Oct;56(5):969-73</RefSource><PMID Version="1">5504534</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Invest. 1986 Jun;77(6):1985-92</RefSource><PMID Version="1">2423560</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 1979 Oct 10;254(19):9747-53</RefSource><PMID 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Version="1">271968</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002009" MajorTopicYN="N">Brugia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005368" MajorTopicYN="N">Filariasis</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005796" MajorTopicYN="N">Genes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005849" MajorTopicYN="N">Gerbillinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC280262</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year><Month>5</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1988</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1988</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">3368467</ArticleId><ArticleId IdType="pmc">PMC280262</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><noCountry><name sortKey="Denker, J A" sort="Denker, J A" uniqKey="Denker J" first="J A" last="Denker">J A Denker</name><name sortKey="Goodwin, R G" sort="Goodwin, R G" uniqKey="Goodwin R" first="R G" last="Goodwin">R G Goodwin</name><name sortKey="Kazura, J W" sort="Kazura, J W" uniqKey="Kazura J" first="J W" last="Kazura">J W Kazura</name><name sortKey="Maroney, P A" sort="Maroney, P A" uniqKey="Maroney P" first="P A" last="Maroney">P A Maroney</name><name sortKey="Nanduri, J" sort="Nanduri, J" uniqKey="Nanduri J" first="J" last="Nanduri">J. Nanduri</name><name sortKey="Perrine, K G" sort="Perrine, K G" uniqKey="Perrine K" first="K G" last="Perrine">K G Perrine</name></noCountry><country name="États-Unis"><region name="Ohio"><name sortKey="Nilsen, T W" sort="Nilsen, T W" uniqKey="Nilsen T" first="T W" last="Nilsen">T W Nilsen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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