[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].
Identifieur interne : 005492 ( PubMed/Checkpoint ); précédent : 005491; suivant : 005493[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].
Auteurs : H J ZhengSource :
- Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [ 0253-9624 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes d'helminthe.
- immunologie : Brugia, Filariose lymphatique, Microfilaria.
- sécrétion : Hybridomes.
- Animaux, Anticorps monoclonaux, Souris, Souris de lignée BALB C, Test ELISA.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Helminth.
- chemical : Antibodies, Monoclonal.
- immunology : Brugia, Elephantiasis, Filarial, Microfilariae.
- methods : Enzyme-Linked Immunosorbent Assay.
- secretion : Hybridomas.
- Animals, Mice, Mice, Inbred BALB C.
Abstract
A hybridoma cell line secreting monoclonal antibody (McAb) against Brugia malayi adult worm excretory-secretory antigen (AWES) was established. The identification of the Ig sub-classes showed that McAb AWES belongs to IgG. We developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to detect circulating parasite antigens in human lymphatic filariasis. 75 of 77 (97.4%) bancroftian microfilaremia cases showed positive reaction. 27 out of 40 (67.5%) microfilarial patients with hydroceles, chyluria, or elephantiasis, and 20% of sera from asymptomatic residents of filariasis-endemic areas evidently contained filarial antigens. 31 of sera from non-endemic area with ascaris infection were all negative. The minimal amount of antigens in pool normal sera adding AWES antigen was detectable at 6.3 pg/ml. The results suggested that Dot-ELISA is a sensitive, specific, cheap and simple agent for use in epidemiological field studies.
PubMed: 2340764
Affiliations:
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pubmed:2340764Le document en format XML
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The identification of the Ig sub-classes showed that McAb AWES belongs to IgG. We developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to detect circulating parasite antigens in human lymphatic filariasis. 75 of 77 (97.4%) bancroftian microfilaremia cases showed positive reaction. 27 out of 40 (67.5%) microfilarial patients with hydroceles, chyluria, or elephantiasis, and 20% of sera from asymptomatic residents of filariasis-endemic areas evidently contained filarial antigens. 31 of sera from non-endemic area with ascaris infection were all negative. The minimal amount of antigens in pool normal sera adding AWES antigen was detectable at 6.3 pg/ml. The results suggested that Dot-ELISA is a sensitive, specific, cheap and simple agent for use in epidemiological field studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2340764</PMID><DateCreated><Year>1990</Year><Month>06</Month><Day>27</Day></DateCreated><DateCompleted><Year>1990</Year><Month>06</Month><Day>27</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0253-9624</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>1</Issue><PubDate><Year>1990</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</Title><ISOAbbreviation>Zhonghua Yu Fang Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].</ArticleTitle><Pagination><MedlinePgn>25-7</MedlinePgn></Pagination><Abstract><AbstractText>A hybridoma cell line secreting monoclonal antibody (McAb) against Brugia malayi adult worm excretory-secretory antigen (AWES) was established. The identification of the Ig sub-classes showed that McAb AWES belongs to IgG. We developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to detect circulating parasite antigens in human lymphatic filariasis. 75 of 77 (97.4%) bancroftian microfilaremia cases showed positive reaction. 27 out of 40 (67.5%) microfilarial patients with hydroceles, chyluria, or elephantiasis, and 20% of sera from asymptomatic residents of filariasis-endemic areas evidently contained filarial antigens. 31 of sera from non-endemic area with ascaris infection were all negative. The minimal amount of antigens in pool normal sera adding AWES antigen was detectable at 6.3 pg/ml. The results suggested that Dot-ELISA is a sensitive, specific, cheap and simple agent for use in epidemiological field studies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>H J</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Guizhou Provincial Institute of Parasitic Diseases, Guiyang.</Affiliation></AffiliationInfo></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Yu Fang Yi Xue Za Zhi</MedlineTA><NlmUniqueID>7904962</NlmUniqueID><ISSNLinking>0253-9624</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="Y">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002009" MajorTopicYN="N">Brugia</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006825" MajorTopicYN="N">Hybridomas</DescriptorName><QualifierName UI="Q000557" MajorTopicYN="N">secretion</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008842" MajorTopicYN="N">Microfilariae</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2340764</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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