Brugia malayi: the diagnostic potential of recombinant excretory/secretory antigens.
Identifieur interne : 004E95 ( PubMed/Checkpoint ); précédent : 004E94; suivant : 004E96Brugia malayi: the diagnostic potential of recombinant excretory/secretory antigens.
Auteurs : S. Kumari [États-Unis] ; C D Lillibridge ; M. Bakeer ; R C Lowrie ; K. Jayaraman ; M T PhilippSource :
- Experimental parasitology [ 0014-4894 ] ; 1994.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Brugia malayi (génétique), Brugia malayi (immunologie), Cercopithecus aethiops, Clonage moléculaire, Données de séquences moléculaires, Femelle, Filariose lymphatique (diagnostic), Humains, Lapins, Macaca mulatta, Mâle, Protéines recombinantes (), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Réactions croisées, Sensibilité et spécificité, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Technique de Western.
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Brugia malayi, Protéines recombinantes.
- immunologie : Antigènes d'helminthe, Brugia malayi, Protéines recombinantes.
- sang : Anticorps antihelminthe.
- Animaux, Antigènes d'helminthe, Cercopithecus aethiops, Clonage moléculaire, Données de séquences moléculaires, Femelle, Humains, Lapins, Macaca mulatta, Mâle, Protéines recombinantes, Réactions croisées, Sensibilité et spécificité, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Technique de Western.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (chemistry), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Base Sequence, Blotting, Western, Brugia malayi (genetics), Brugia malayi (immunology), Cercopithecus aethiops, Cloning, Molecular, Cross Reactions, Elephantiasis, Filarial (diagnosis), Female, Humans, Macaca mulatta, Male, Molecular Sequence Data, Rabbits, Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Sensitivity and Specificity, Sequence Homology, Amino Acid.
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , chemistry : Antigens, Helminth, Recombinant Proteins.
- chemical , genetics : Antigens, Helminth, Recombinant Proteins.
- chemical , immunology : Antigens, Helminth, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- genetics : Brugia malayi.
- immunology : Brugia malayi.
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, Cercopithecus aethiops, Cloning, Molecular, Cross Reactions, Female, Humans, Macaca mulatta, Male, Molecular Sequence Data, Rabbits, Sensitivity and Specificity, Sequence Homology, Amino Acid.
Abstract
The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from B. malayi male and female adult worms. Two of these recombinants, Bm12 and Bm14L, were studied after subcloning the cDNA inserts in an Escherichia coli plasmid expression and purification vector, obtaining the inserts' nucleotide sequence, and purifying the expressed proteins. By homology of their deduced amino acid sequence with that of previously identified proteins, Bm12 was identified as the B. malayi gp 15/400 antigen, and Bm14 as a member of the hsp90 family of heat shock proteins. The antigenic cross-reactivity of the purified recombinant proteins was assessed with 28 serum samples from patients infected with Ascaris, Trichuris, or hookworm, and also with a few samples from patients with onchocerciasis and loiasis. For Bm12, the specificity for all of the intestinal helminthiasis together was 75%. Bm14L, on the other hand, cross-reacted with all of the ascariasis serum samples with which it was tested. Presence of antibodies cross-reactive with B. malayi was confirmed in all of these serum samples by examining their antibody reactivity with Western blots of extracts of whole B. malayi adult worms. A semiquantitative (+ or -) assessment of the sensitivity of Bm12 for antibody detection was performed using 6 serum samples from patients with chronic filariasis and 24 samples from patients with microfilaremia. All of these serum samples contained anti-Bm12 antibody (sensitivity of 100%). Finally, the ability of Bm12 to detect antibody before the onset of patency was established with a longitudinal collection of serum samples obtained from 2 African green vervets (Cercopithecus aethiops) and 3 rhesus macaques (Macaca mulatta), all of which were infected with B. malayi. Anti-Bm12 antibodies were detectable in all animals between 4 and 11 weeks before patency.
DOI: 10.1006/expr.1994.1110
PubMed: 8001660
Affiliations:
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Kumari</name><affiliation wicri:level="2"><nlm:affiliation>Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington</wicri:cityArea></affiliation></author><author><name sortKey="Lillibridge, C D" sort="Lillibridge, C D" uniqKey="Lillibridge C" first="C D" last="Lillibridge">C D Lillibridge</name></author><author><name sortKey="Bakeer, M" sort="Bakeer, M" uniqKey="Bakeer M" first="M" last="Bakeer">M. Bakeer</name></author><author><name sortKey="Lowrie, R C" sort="Lowrie, R C" uniqKey="Lowrie R" first="R C" last="Lowrie">R C Lowrie</name></author><author><name sortKey="Jayaraman, K" sort="Jayaraman, K" uniqKey="Jayaraman K" first="K" last="Jayaraman">K. Jayaraman</name></author><author><name sortKey="Philipp, M T" sort="Philipp, M T" uniqKey="Philipp M" first="M T" last="Philipp">M T Philipp</name></author></analytic><series><title level="j">Experimental parasitology</title><idno type="ISSN">0014-4894</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (chemistry)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Base Sequence</term><term>Blotting, Western</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (immunology)</term><term>Cercopithecus aethiops</term><term>Cloning, Molecular</term><term>Cross Reactions</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Female</term><term>Humans</term><term>Macaca mulatta</term><term>Male</term><term>Molecular Sequence Data</term><term>Rabbits</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Sensitivity and Specificity</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe ()</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (immunologie)</term><term>Cercopithecus aethiops</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Filariose lymphatique (diagnostic)</term><term>Humains</term><term>Lapins</term><term>Macaca mulatta</term><term>Mâle</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Réactions croisées</term><term>Sensibilité et spécificité</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Western</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Blotting, Western</term><term>Cercopithecus aethiops</term><term>Cloning, Molecular</term><term>Cross Reactions</term><term>Female</term><term>Humans</term><term>Macaca mulatta</term><term>Male</term><term>Molecular Sequence Data</term><term>Rabbits</term><term>Sensitivity and Specificity</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigènes d'helminthe</term><term>Cercopithecus aethiops</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Humains</term><term>Lapins</term><term>Macaca mulatta</term><term>Mâle</term><term>Protéines recombinantes</term><term>Réactions croisées</term><term>Sensibilité et spécificité</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from B. malayi male and female adult worms. Two of these recombinants, Bm12 and Bm14L, were studied after subcloning the cDNA inserts in an Escherichia coli plasmid expression and purification vector, obtaining the inserts' nucleotide sequence, and purifying the expressed proteins. By homology of their deduced amino acid sequence with that of previously identified proteins, Bm12 was identified as the B. malayi gp 15/400 antigen, and Bm14 as a member of the hsp90 family of heat shock proteins. The antigenic cross-reactivity of the purified recombinant proteins was assessed with 28 serum samples from patients infected with Ascaris, Trichuris, or hookworm, and also with a few samples from patients with onchocerciasis and loiasis. For Bm12, the specificity for all of the intestinal helminthiasis together was 75%. Bm14L, on the other hand, cross-reacted with all of the ascariasis serum samples with which it was tested. Presence of antibodies cross-reactive with B. malayi was confirmed in all of these serum samples by examining their antibody reactivity with Western blots of extracts of whole B. malayi adult worms. A semiquantitative (+ or -) assessment of the sensitivity of Bm12 for antibody detection was performed using 6 serum samples from patients with chronic filariasis and 24 samples from patients with microfilaremia. All of these serum samples contained anti-Bm12 antibody (sensitivity of 100%). Finally, the ability of Bm12 to detect antibody before the onset of patency was established with a longitudinal collection of serum samples obtained from 2 African green vervets (Cercopithecus aethiops) and 3 rhesus macaques (Macaca mulatta), all of which were infected with B. malayi. Anti-Bm12 antibodies were detectable in all animals between 4 and 11 weeks before patency.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8001660</PMID><DateCreated><Year>1995</Year><Month>01</Month><Day>23</Day></DateCreated><DateCompleted><Year>1995</Year><Month>01</Month><Day>23</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-4894</ISSN><JournalIssue CitedMedium="Print"><Volume>79</Volume><Issue>4</Issue><PubDate><Year>1994</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Experimental parasitology</Title><ISOAbbreviation>Exp. Parasitol.</ISOAbbreviation></Journal><ArticleTitle>Brugia malayi: the diagnostic potential of recombinant excretory/secretory antigens.</ArticleTitle><Pagination><MedlinePgn>489-505</MedlinePgn></Pagination><Abstract><AbstractText>The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from B. malayi male and female adult worms. Two of these recombinants, Bm12 and Bm14L, were studied after subcloning the cDNA inserts in an Escherichia coli plasmid expression and purification vector, obtaining the inserts' nucleotide sequence, and purifying the expressed proteins. By homology of their deduced amino acid sequence with that of previously identified proteins, Bm12 was identified as the B. malayi gp 15/400 antigen, and Bm14 as a member of the hsp90 family of heat shock proteins. The antigenic cross-reactivity of the purified recombinant proteins was assessed with 28 serum samples from patients infected with Ascaris, Trichuris, or hookworm, and also with a few samples from patients with onchocerciasis and loiasis. For Bm12, the specificity for all of the intestinal helminthiasis together was 75%. Bm14L, on the other hand, cross-reacted with all of the ascariasis serum samples with which it was tested. Presence of antibodies cross-reactive with B. malayi was confirmed in all of these serum samples by examining their antibody reactivity with Western blots of extracts of whole B. malayi adult worms. A semiquantitative (+ or -) assessment of the sensitivity of Bm12 for antibody detection was performed using 6 serum samples from patients with chronic filariasis and 24 samples from patients with microfilaremia. All of these serum samples contained anti-Bm12 antibody (sensitivity of 100%). Finally, the ability of Bm12 to detect antibody before the onset of patency was established with a longitudinal collection of serum samples obtained from 2 African green vervets (Cercopithecus aethiops) and 3 rhesus macaques (Macaca mulatta), all of which were infected with B. malayi. Anti-Bm12 antibodies were detectable in all animals between 4 and 11 weeks before patency.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kumari</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lillibridge</LastName><ForeName>C D</ForeName><Initials>CD</Initials></Author><Author ValidYN="Y"><LastName>Bakeer</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Lowrie</LastName><ForeName>R C</ForeName><Initials>RC</Initials><Suffix>Jr</Suffix></Author><Author ValidYN="Y"><LastName>Jayaraman</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Philipp</LastName><ForeName>M T</ForeName><Initials>MT</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>S76365</AccessionNumber><AccessionNumber>S76367</AccessionNumber></AccessionNumberList></DataBank></DataBankList><GrantList CompleteYN="Y"><Grant><GrantID>RRO164</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Exp Parasitol</MedlineTA><NlmUniqueID>0370713</NlmUniqueID><ISSNLinking>0014-4894</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Cercopithecus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003429" MajorTopicYN="N">Cross Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1994</Year><Month>12</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1994</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8001660</ArticleId><ArticleId IdType="pii">S0014-4894(84)71110-6</ArticleId><ArticleId IdType="doi">10.1006/expr.1994.1110</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Louisiane</li></region></list><tree><noCountry><name sortKey="Bakeer, M" sort="Bakeer, M" uniqKey="Bakeer M" first="M" last="Bakeer">M. Bakeer</name><name sortKey="Jayaraman, K" sort="Jayaraman, K" uniqKey="Jayaraman K" first="K" last="Jayaraman">K. Jayaraman</name><name sortKey="Lillibridge, C D" sort="Lillibridge, C D" uniqKey="Lillibridge C" first="C D" last="Lillibridge">C D Lillibridge</name><name sortKey="Lowrie, R C" sort="Lowrie, R C" uniqKey="Lowrie R" first="R C" last="Lowrie">R C Lowrie</name><name sortKey="Philipp, M T" sort="Philipp, M T" uniqKey="Philipp M" first="M T" last="Philipp">M T Philipp</name></noCountry><country name="États-Unis"><region name="Louisiane"><name sortKey="Kumari, S" sort="Kumari, S" uniqKey="Kumari S" first="S" last="Kumari">S. Kumari</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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