An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.
Identifieur interne : 002D39 ( PubMed/Checkpoint ); précédent : 002D38; suivant : 002D40An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.
Auteurs : S L Hoti [Inde] ; R. Dhamodharan ; K. Subramaniyan ; P K DasSource :
- The Indian journal of medical research [ 0971-5916 ] ; 2009.
Descripteurs français
- KwdFr :
- Albendazole (pharmacologie), Albendazole (usage thérapeutique), Alignement de séquences, Allèles, Animaux, Données de séquences moléculaires, Filaricides (pharmacologie), Filaricides (usage thérapeutique), Filariose lymphatique (parasitologie), Filariose lymphatique (traitement médicamenteux), Humains, Polymorphisme génétique, Réaction de polymérisation en chaîne (), Résistance aux substances (génétique), Séquence d'acides aminés, Séquence nucléotidique, Tests de sensibilité parasitaire, Tubuline (génétique), Wuchereria bancrofti (), Wuchereria bancrofti (génétique), Wuchereria bancrofti (physiologie).
- MESH :
- génétique : Résistance aux substances, Tubuline, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- pharmacologie : Albendazole, Filaricides.
- physiologie : Wuchereria bancrofti.
- traitement médicamenteux : Filariose lymphatique.
- usage thérapeutique : Albendazole, Filaricides.
- Alignement de séquences, Allèles, Animaux, Données de séquences moléculaires, Humains, Polymorphisme génétique, Réaction de polymérisation en chaîne, Séquence d'acides aminés, Séquence nucléotidique, Tests de sensibilité parasitaire, Wuchereria bancrofti.
English descriptors
- KwdEn :
- Albendazole (pharmacology), Albendazole (therapeutic use), Alleles, Amino Acid Sequence, Animals, Base Sequence, Drug Resistance (genetics), Elephantiasis, Filarial (drug therapy), Elephantiasis, Filarial (parasitology), Filaricides (pharmacology), Filaricides (therapeutic use), Humans, Molecular Sequence Data, Parasitic Sensitivity Tests, Polymerase Chain Reaction (methods), Polymorphism, Genetic, Sequence Alignment, Tubulin (genetics), Wuchereria bancrofti (drug effects), Wuchereria bancrofti (genetics), Wuchereria bancrofti (physiology).
- MESH :
- chemical , genetics : Tubulin.
- chemical , pharmacology : Albendazole, Filaricides.
- chemical , therapeutic use : Albendazole, Filaricides.
- drug effects : Wuchereria bancrofti.
- drug therapy : Elephantiasis, Filarial.
- genetics : Drug Resistance, Wuchereria bancrofti.
- methods : Polymerase Chain Reaction.
- parasitology : Elephantiasis, Filarial.
- physiology : Wuchereria bancrofti.
- Alleles, Amino Acid Sequence, Animals, Base Sequence, Humans, Molecular Sequence Data, Parasitic Sensitivity Tests, Polymorphism, Genetic, Sequence Alignment.
Abstract
Albendazole, a commonly used anthelminthic drug that targets the polymerization of alpha- and beta-tubulin dimer is currently co-administered with the antifilarial drug, diethylcarbamazine citrate (DEC) in the ongoing Global Programme for Elimination of Lymphatic Filariasis (GPELF). The experience in veterinary field has shown that there can be a rapid development of resistance to this drug, which therefore, needs to be monitored regularly in GPELF. Hence, we investigated the nucleotide polymorphism in the albendazole-binding domain of the isotype 1 beta-tubulin gene from several populations of Wuchereria bancrofti and developed an AS-PCR assay useful in screening for sensitive/resistance alleles among parasite populations and also evaluated its utility.
PubMed: 19797818
Affiliations:
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pubmed:19797818Le document en format XML
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<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Drug Resistance (genetics)</term>
<term>Elephantiasis, Filarial (drug therapy)</term>
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<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Parasitic Sensitivity Tests</term>
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<term>Polymorphism, Genetic</term>
<term>Sequence Alignment</term>
<term>Tubulin (genetics)</term>
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<term>Albendazole (usage thérapeutique)</term>
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<term>Allèles</term>
<term>Animaux</term>
<term>Données de séquences moléculaires</term>
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<term>Filaricides (usage thérapeutique)</term>
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<term>Filariose lymphatique (traitement médicamenteux)</term>
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<term>Polymorphisme génétique</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Résistance aux substances (génétique)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Tests de sensibilité parasitaire</term>
<term>Tubuline (génétique)</term>
<term>Wuchereria bancrofti ()</term>
<term>Wuchereria bancrofti (génétique)</term>
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<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Résistance aux substances</term>
<term>Tubuline</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
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<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term>
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<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Albendazole</term>
<term>Filaricides</term>
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<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
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<term>Allèles</term>
<term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Polymorphisme génétique</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">Albendazole, a commonly used anthelminthic drug that targets the polymerization of alpha- and beta-tubulin dimer is currently co-administered with the antifilarial drug, diethylcarbamazine citrate (DEC) in the ongoing Global Programme for Elimination of Lymphatic Filariasis (GPELF). The experience in veterinary field has shown that there can be a rapid development of resistance to this drug, which therefore, needs to be monitored regularly in GPELF. Hence, we investigated the nucleotide polymorphism in the albendazole-binding domain of the isotype 1 beta-tubulin gene from several populations of Wuchereria bancrofti and developed an AS-PCR assay useful in screening for sensitive/resistance alleles among parasite populations and also evaluated its utility.</div>
</front>
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<DateCreated><Year>2009</Year>
<Month>10</Month>
<Day>02</Day>
</DateCreated>
<DateCompleted><Year>2009</Year>
<Month>12</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0971-5916</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>130</Volume>
<Issue>2</Issue>
<PubDate><Year>2009</Year>
<Month>Aug</Month>
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<Title>The Indian journal of medical research</Title>
<ISOAbbreviation>Indian J. Med. Res.</ISOAbbreviation>
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<ArticleTitle>An allele specific PCR assay for screening for drug resistance among Wuchereria bancrofti populations in India.</ArticleTitle>
<Pagination><MedlinePgn>193-9</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND & OBJECTIVE" NlmCategory="OBJECTIVE">Albendazole, a commonly used anthelminthic drug that targets the polymerization of alpha- and beta-tubulin dimer is currently co-administered with the antifilarial drug, diethylcarbamazine citrate (DEC) in the ongoing Global Programme for Elimination of Lymphatic Filariasis (GPELF). The experience in veterinary field has shown that there can be a rapid development of resistance to this drug, which therefore, needs to be monitored regularly in GPELF. Hence, we investigated the nucleotide polymorphism in the albendazole-binding domain of the isotype 1 beta-tubulin gene from several populations of Wuchereria bancrofti and developed an AS-PCR assay useful in screening for sensitive/resistance alleles among parasite populations and also evaluated its utility.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">For studying the polymorphism of isotype 1 beta-tubulin gene, a 475 bp fragment spanning exon 5 and 6 of the gene was amplified and sequenced from the genomic DNA of W. bancrofti collected from six geographic regions of India. An allele specific (AS) PCR for screening albendazole sensitivity/resistance was developed and a total of 55 mf samples from blood smears on slides collected from Thiruvannamalai, Thanjavur and Puducherry were screened. Selective therapy with DEC was in place in three areas, mass drug administration (MDA) with DEC alone was implemented in four areas, while DEC plus albendazole was administered in one district.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The analysis of the nucleotide sequence of the fragment from 20 W. bancrofti populations showed the domain to be highly conserved. An allele-specific PCR assay developed was used to detect sensitive/resistance alleles among 55 isolates of W. bancrofti and no albendazole resistance alleles were detected among the populations tested.</AbstractText>
<AbstractText Label="INTERPRETATION & CONCLUSION" NlmCategory="CONCLUSIONS">The drug-binding domain of isotype 1 beta-tubulin gene of W. bancrofti from different geographical locations was highly conserved. The AS-PCR developed showed potential application as a tool for monitoring albendazole sensitivity/resistance alleles among W. bancrofti populations, in areas where combination therapy of DEC-albendazole is being mass administered in the LF elimination programme.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hoti</LastName>
<ForeName>S L</ForeName>
<Initials>SL</Initials>
<AffiliationInfo><Affiliation>Vector Control Research Centre (ICMR), Puducherry, India. slhoti@yahoo.com</Affiliation>
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<Author ValidYN="Y"><LastName>Dhamodharan</LastName>
<ForeName>R</ForeName>
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<Author ValidYN="Y"><LastName>Subramaniyan</LastName>
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<Author ValidYN="Y"><LastName>Das</LastName>
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<NlmUniqueID>0374701</NlmUniqueID>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D015766" MajorTopicYN="N">Albendazole</DescriptorName>
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<MeshHeading><DescriptorName UI="D000483" MajorTopicYN="Y">Alleles</DescriptorName>
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<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
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<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
<QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
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<MeshHeading><DescriptorName UI="D021261" MajorTopicYN="N">Parasitic Sensitivity Tests</DescriptorName>
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<MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011110" MajorTopicYN="Y">Polymorphism, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
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<MeshHeading><DescriptorName UI="D014404" MajorTopicYN="N">Tubulin</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
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