DNA-based diagnosis of lymphatic filariasis.
Identifieur interne : 002C95 ( PubMed/Checkpoint ); précédent : 002C94; suivant : 002C96DNA-based diagnosis of lymphatic filariasis.
Auteurs : Surang Nuchprayoon [Thaïlande]Source :
- The Southeast Asian journal of tropical medicine and public health [ 0125-1562 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN des helminthes (isolement et purification), Animaux, Antigènes d'helminthe (sang), Brugia malayi (génétique), Brugia malayi (isolement et purification), Filariose lymphatique (diagnostic), Filariose lymphatique (ethnologie), Filariose lymphatique (parasitologie), Humains, Thaïlande (épidémiologie), Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification).
- MESH :
- diagnostic : Filariose lymphatique.
- ethnologie : Filariose lymphatique.
- génétique : Brugia malayi, Wuchereria bancrofti.
- isolement et purification : ADN des helminthes, Brugia malayi, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Antigènes d'helminthe.
- épidémiologie : Thaïlande.
- Animaux, Humains.
- Wicri :
- geographic : Thaïlande.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (blood), Brugia malayi (genetics), Brugia malayi (isolation & purification), DNA, Helminth (isolation & purification), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (ethnology), Elephantiasis, Filarial (parasitology), Humans, Thailand (epidemiology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , blood : Antigens, Helminth.
- geographic , epidemiology : Thailand.
- diagnosis : Elephantiasis, Filarial.
- ethnology : Elephantiasis, Filarial.
- genetics : Brugia malayi, Wuchereria bancrofti.
- isolation & purification : Brugia malayi, DNA, Helminth, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Humans.
Abstract
Lymphatic filariasis (LF) is still a major public health problem. The disease is ranked by the World Health Organization (WHO) as the second leading cause of permanent and long-term disability, and has been targeted for elimination by 2020. Effective diagnosis LF is required for treatment of infected individuals, for epidemiological assessment and for monitoring of the control program. Conventional diagnosis of LF depends on detection of microfilariae (Mf) in blood specimens, which has low sensitivity and specificity. Detection of specific circulating filarial antigens is regarded by WHO as the 'gold standard' for diagnosis of LF. However, the limitations of the antigen tests are cost and inconsistent availability. Although anti-filarial IgG4 antibody levels are associated with active LF infections, however, cross-reactivity with other filarial parasites is common. Not as sensitive as antigen tests, DNA-based techniques have been developed to diagnose and differentiate filarial parasites in humans, animal reservoir hosts, and mosquito vectors. These include DNA hybridization, polymerase chain reaction (PCR) amplification using specific primers (eg Ssp I repeat, pWb12 repeat, pWb-35 repeat, and LDR repeat for Wuchereria bancrofti and Hha I repeat, glutathione peroxidase gene, mitochondrial DNA for Brugia malayi), and universal primers, multiplex-PCR, PCR-restriction fragment length polymorphism (PCR-RFLP), PCR-enzyme linked immunosorbent assay (PCR-ELISA), as well as quantitative PCR. Furthermore, because bancroftian filariasis is endemic on the Thai-Myanmar border, the potential now exists for a re-emergence of bancroftian filariasis in Thailand, and random amplified polymorphic DNA (RAPD) analysis has proved effective to differentiate Thai and Myanmar strains of W. bancrofti.
PubMed: 19842372
Affiliations:
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Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Thaïlande</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Thaïlande</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis (LF) is still a major public health problem. The disease is ranked by the World Health Organization (WHO) as the second leading cause of permanent and long-term disability, and has been targeted for elimination by 2020. Effective diagnosis LF is required for treatment of infected individuals, for epidemiological assessment and for monitoring of the control program. Conventional diagnosis of LF depends on detection of microfilariae (Mf) in blood specimens, which has low sensitivity and specificity. Detection of specific circulating filarial antigens is regarded by WHO as the 'gold standard' for diagnosis of LF. However, the limitations of the antigen tests are cost and inconsistent availability. Although anti-filarial IgG4 antibody levels are associated with active LF infections, however, cross-reactivity with other filarial parasites is common. Not as sensitive as antigen tests, DNA-based techniques have been developed to diagnose and differentiate filarial parasites in humans, animal reservoir hosts, and mosquito vectors. These include DNA hybridization, polymerase chain reaction (PCR) amplification using specific primers (eg Ssp I repeat, pWb12 repeat, pWb-35 repeat, and LDR repeat for Wuchereria bancrofti and Hha I repeat, glutathione peroxidase gene, mitochondrial DNA for Brugia malayi), and universal primers, multiplex-PCR, PCR-restriction fragment length polymorphism (PCR-RFLP), PCR-enzyme linked immunosorbent assay (PCR-ELISA), as well as quantitative PCR. Furthermore, because bancroftian filariasis is endemic on the Thai-Myanmar border, the potential now exists for a re-emergence of bancroftian filariasis in Thailand, and random amplified polymorphic DNA (RAPD) analysis has proved effective to differentiate Thai and Myanmar strains of W. bancrofti.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19842372</PMID><DateCreated><Year>2009</Year><Month>10</Month><Day>21</Day></DateCreated><DateCompleted><Year>2009</Year><Month>11</Month><Day>24</Day></DateCompleted><DateRevised><Year>2009</Year><Month>10</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0125-1562</ISSN><JournalIssue CitedMedium="Print"><Volume>40</Volume><Issue>5</Issue><PubDate><Year>2009</Year><Month>Sep</Month></PubDate></JournalIssue><Title>The Southeast Asian journal of tropical medicine and public health</Title><ISOAbbreviation>Southeast Asian J. Trop. Med. Public Health</ISOAbbreviation></Journal><ArticleTitle>DNA-based diagnosis of lymphatic filariasis.</ArticleTitle><Pagination><MedlinePgn>904-13</MedlinePgn></Pagination><Abstract><AbstractText>Lymphatic filariasis (LF) is still a major public health problem. The disease is ranked by the World Health Organization (WHO) as the second leading cause of permanent and long-term disability, and has been targeted for elimination by 2020. Effective diagnosis LF is required for treatment of infected individuals, for epidemiological assessment and for monitoring of the control program. Conventional diagnosis of LF depends on detection of microfilariae (Mf) in blood specimens, which has low sensitivity and specificity. Detection of specific circulating filarial antigens is regarded by WHO as the 'gold standard' for diagnosis of LF. However, the limitations of the antigen tests are cost and inconsistent availability. Although anti-filarial IgG4 antibody levels are associated with active LF infections, however, cross-reactivity with other filarial parasites is common. Not as sensitive as antigen tests, DNA-based techniques have been developed to diagnose and differentiate filarial parasites in humans, animal reservoir hosts, and mosquito vectors. These include DNA hybridization, polymerase chain reaction (PCR) amplification using specific primers (eg Ssp I repeat, pWb12 repeat, pWb-35 repeat, and LDR repeat for Wuchereria bancrofti and Hha I repeat, glutathione peroxidase gene, mitochondrial DNA for Brugia malayi), and universal primers, multiplex-PCR, PCR-restriction fragment length polymorphism (PCR-RFLP), PCR-enzyme linked immunosorbent assay (PCR-ELISA), as well as quantitative PCR. Furthermore, because bancroftian filariasis is endemic on the Thai-Myanmar border, the potential now exists for a re-emergence of bancroftian filariasis in Thailand, and random amplified polymorphic DNA (RAPD) analysis has proved effective to differentiate Thai and Myanmar strains of W. bancrofti.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nuchprayoon</LastName><ForeName>Surang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. fmedstt@md2.md.chula.ac.th</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016454">Review</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Thailand</Country><MedlineTA>Southeast Asian J Trop Med Public Health</MedlineTA><NlmUniqueID>0266303</NlmUniqueID><ISSNLinking>0125-1562</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018509">DNA, Helminth</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018509" MajorTopicYN="N">DNA, Helminth</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000208" MajorTopicYN="N">ethnology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013785" MajorTopicYN="N" Type="Geographic">Thailand</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList><NumberOfReferences>49</NumberOfReferences></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19842372</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Nuchprayoon, Surang" sort="Nuchprayoon, Surang" uniqKey="Nuchprayoon S" first="Surang" last="Nuchprayoon">Surang Nuchprayoon</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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