Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.
Identifieur interne : 002B62 ( PubMed/Checkpoint ); précédent : 002B61; suivant : 002B63Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.
Auteurs : Claudius Conrad [États-Unis] ; Hanno Niess ; Ralf Huss ; Stephan Huber ; Irene Von Luettichau ; Peter J. Nelson ; Harald C. Ott ; Karl-Walter Jauch ; Christiane J. BrunsSource :
- Circulation [ 1524-4539 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Cellules souches multipotentes (cytologie), Cellules souches multipotentes (physiologie), Cellules stromales mésenchymateuses (cytologie), Cellules stromales mésenchymateuses (physiologie), Différenciation cellulaire (physiologie), Endothélium lymphatique (cytologie), Endothélium lymphatique (physiologie), Femelle, Humains, Lignée cellulaire, Lymphangiogenèse (physiologie), Mouvement cellulaire (physiologie), Phénotype, Régénération (physiologie), Souris, Souris de lignée BALB C, Souris de lignée C57BL, Vaisseaux lymphatiques (cytologie), Vaisseaux lymphatiques (physiologie).
- MESH :
- cytologie : Cellules souches multipotentes, Cellules stromales mésenchymateuses, Endothélium lymphatique, Vaisseaux lymphatiques.
- physiologie : Cellules souches multipotentes, Cellules stromales mésenchymateuses, Différenciation cellulaire, Endothélium lymphatique, Lymphangiogenèse, Mouvement cellulaire, Régénération, Vaisseaux lymphatiques.
- Animaux, Femelle, Humains, Lignée cellulaire, Phénotype, Souris, Souris de lignée BALB C, Souris de lignée C57BL.
English descriptors
- KwdEn :
- Animals, Cell Differentiation (physiology), Cell Line, Cell Movement (physiology), Endothelium, Lymphatic (cytology), Endothelium, Lymphatic (physiology), Female, Humans, Lymphangiogenesis (physiology), Lymphatic Vessels (cytology), Lymphatic Vessels (physiology), Mesenchymal Stromal Cells (cytology), Mesenchymal Stromal Cells (physiology), Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Multipotent Stem Cells (cytology), Multipotent Stem Cells (physiology), Phenotype, Regeneration (physiology).
- MESH :
- cytology : Endothelium, Lymphatic, Lymphatic Vessels, Mesenchymal Stromal Cells, Multipotent Stem Cells.
- physiology : Cell Differentiation, Cell Movement, Endothelium, Lymphatic, Lymphangiogenesis, Lymphatic Vessels, Mesenchymal Stromal Cells, Multipotent Stem Cells, Regeneration.
- Animals, Cell Line, Female, Humans, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Phenotype.
Abstract
The importance and therapeutic value of stem cells in lymphangiogenesis are poorly understood. We evaluated the potential of human and murine mesenchymal stem cells (MSCs) to acquire a lymphatic phenotype in vitro and to enhance lymphatic regeneration in vivo.
DOI: 10.1161/CIRCULATIONAHA.108.793208
PubMed: 19118255
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:19118255Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.</title><author><name sortKey="Conrad, Claudius" sort="Conrad, Claudius" uniqKey="Conrad C" first="Claudius" last="Conrad">Claudius Conrad</name><affiliation wicri:level="2"><nlm:affiliation>Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. CConrad1@partners.org</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Niess, Hanno" sort="Niess, Hanno" uniqKey="Niess H" first="Hanno" last="Niess">Hanno Niess</name></author><author><name sortKey="Huss, Ralf" sort="Huss, Ralf" uniqKey="Huss R" first="Ralf" last="Huss">Ralf Huss</name></author><author><name sortKey="Huber, Stephan" sort="Huber, Stephan" uniqKey="Huber S" first="Stephan" last="Huber">Stephan Huber</name></author><author><name sortKey="Von Luettichau, Irene" sort="Von Luettichau, Irene" uniqKey="Von Luettichau I" first="Irene" last="Von Luettichau">Irene Von Luettichau</name></author><author><name sortKey="Nelson, Peter J" sort="Nelson, Peter J" uniqKey="Nelson P" first="Peter J" last="Nelson">Peter J. Nelson</name></author><author><name sortKey="Ott, Harald C" sort="Ott, Harald C" uniqKey="Ott H" first="Harald C" last="Ott">Harald C. Ott</name></author><author><name sortKey="Jauch, Karl Walter" sort="Jauch, Karl Walter" uniqKey="Jauch K" first="Karl-Walter" last="Jauch">Karl-Walter Jauch</name></author><author><name sortKey="Bruns, Christiane J" sort="Bruns, Christiane J" uniqKey="Bruns C" first="Christiane J" last="Bruns">Christiane J. Bruns</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19118255</idno><idno type="pmid">19118255</idno><idno type="doi">10.1161/CIRCULATIONAHA.108.793208</idno><idno type="wicri:Area/PubMed/Corpus">003019</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003019</idno><idno type="wicri:Area/PubMed/Curation">003019</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003019</idno><idno type="wicri:Area/PubMed/Checkpoint">003019</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003019</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.</title><author><name sortKey="Conrad, Claudius" sort="Conrad, Claudius" uniqKey="Conrad C" first="Claudius" last="Conrad">Claudius Conrad</name><affiliation wicri:level="2"><nlm:affiliation>Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. CConrad1@partners.org</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Niess, Hanno" sort="Niess, Hanno" uniqKey="Niess H" first="Hanno" last="Niess">Hanno Niess</name></author><author><name sortKey="Huss, Ralf" sort="Huss, Ralf" uniqKey="Huss R" first="Ralf" last="Huss">Ralf Huss</name></author><author><name sortKey="Huber, Stephan" sort="Huber, Stephan" uniqKey="Huber S" first="Stephan" last="Huber">Stephan Huber</name></author><author><name sortKey="Von Luettichau, Irene" sort="Von Luettichau, Irene" uniqKey="Von Luettichau I" first="Irene" last="Von Luettichau">Irene Von Luettichau</name></author><author><name sortKey="Nelson, Peter J" sort="Nelson, Peter J" uniqKey="Nelson P" first="Peter J" last="Nelson">Peter J. Nelson</name></author><author><name sortKey="Ott, Harald C" sort="Ott, Harald C" uniqKey="Ott H" first="Harald C" last="Ott">Harald C. Ott</name></author><author><name sortKey="Jauch, Karl Walter" sort="Jauch, Karl Walter" uniqKey="Jauch K" first="Karl-Walter" last="Jauch">Karl-Walter Jauch</name></author><author><name sortKey="Bruns, Christiane J" sort="Bruns, Christiane J" uniqKey="Bruns C" first="Christiane J" last="Bruns">Christiane J. Bruns</name></author></analytic><series><title level="j">Circulation</title><idno type="eISSN">1524-4539</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Differentiation (physiology)</term><term>Cell Line</term><term>Cell Movement (physiology)</term><term>Endothelium, Lymphatic (cytology)</term><term>Endothelium, Lymphatic (physiology)</term><term>Female</term><term>Humans</term><term>Lymphangiogenesis (physiology)</term><term>Lymphatic Vessels (cytology)</term><term>Lymphatic Vessels (physiology)</term><term>Mesenchymal Stromal Cells (cytology)</term><term>Mesenchymal Stromal Cells (physiology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Multipotent Stem Cells (cytology)</term><term>Multipotent Stem Cells (physiology)</term><term>Phenotype</term><term>Regeneration (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules souches multipotentes (cytologie)</term><term>Cellules souches multipotentes (physiologie)</term><term>Cellules stromales mésenchymateuses (cytologie)</term><term>Cellules stromales mésenchymateuses (physiologie)</term><term>Différenciation cellulaire (physiologie)</term><term>Endothélium lymphatique (cytologie)</term><term>Endothélium lymphatique (physiologie)</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Lymphangiogenèse (physiologie)</term><term>Mouvement cellulaire (physiologie)</term><term>Phénotype</term><term>Régénération (physiologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Vaisseaux lymphatiques (cytologie)</term><term>Vaisseaux lymphatiques (physiologie)</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules souches multipotentes</term><term>Cellules stromales mésenchymateuses</term><term>Endothélium lymphatique</term><term>Vaisseaux lymphatiques</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Endothelium, Lymphatic</term><term>Lymphatic Vessels</term><term>Mesenchymal Stromal Cells</term><term>Multipotent Stem Cells</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules souches multipotentes</term><term>Cellules stromales mésenchymateuses</term><term>Différenciation cellulaire</term><term>Endothélium lymphatique</term><term>Lymphangiogenèse</term><term>Mouvement cellulaire</term><term>Régénération</term><term>Vaisseaux lymphatiques</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Differentiation</term><term>Cell Movement</term><term>Endothelium, Lymphatic</term><term>Lymphangiogenesis</term><term>Lymphatic Vessels</term><term>Mesenchymal Stromal Cells</term><term>Multipotent Stem Cells</term><term>Regeneration</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Phénotype</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The importance and therapeutic value of stem cells in lymphangiogenesis are poorly understood. We evaluated the potential of human and murine mesenchymal stem cells (MSCs) to acquire a lymphatic phenotype in vitro and to enhance lymphatic regeneration in vivo.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19118255</PMID><DateCreated><Year>2009</Year><Month>01</Month><Day>20</Day></DateCreated><DateCompleted><Year>2009</Year><Month>02</Month><Day>13</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1524-4539</ISSN><JournalIssue CitedMedium="Internet"><Volume>119</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Jan</Month><Day>20</Day></PubDate></JournalIssue><Title>Circulation</Title><ISOAbbreviation>Circulation</ISOAbbreviation></Journal><ArticleTitle>Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.</ArticleTitle><Pagination><MedlinePgn>281-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1161/CIRCULATIONAHA.108.793208</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The importance and therapeutic value of stem cells in lymphangiogenesis are poorly understood. We evaluated the potential of human and murine mesenchymal stem cells (MSCs) to acquire a lymphatic phenotype in vitro and to enhance lymphatic regeneration in vivo.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">We assessed the lymphendothelial differentiation of human and murine MSCs after induction with supernatant derived from human dermal microvascular endothelial cells, isolated lymphatic endothelial cells, and purified vascular endothelial growth factor (VEGF)-C in vitro. We used human or murine progenitor MSC lines and then characterized the lymphatic phenotype by morphology, migratory capacity, and the expression of lymphatic markers such as Prox-1, podoplanin, Lyve-1, VEGF receptor-2, and VEGF receptor-3. Using a murine lymphatic edema model, we assessed the potential of these cells to form a functional lymphatic vasculature in vivo after injection of syngeneic MSCs. Incubation with supernatant from lymphatic endothelial cells induced an endothelium-like morphology and the expression of lymphendothelial markers in both human and murine MSCs in vitro. MSCs showed migratory activity along a VEGF-C gradient, which was enhanced by VEGF-C conditioning. In vivo, the local application of MSCs resulted in a significant decrease in edema formation (-20.1%; P<0.01 versus untreated tails) after 3 weekly cell injections and restored the drainage of intradermally injected methylene blue after 7 weekly injections.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">MSCs were capable of expressing a lymphatic phenotype when exposed to lymph-inductive media and purified VEGF-C. Migratory activity toward VEGF-C in vitro suggests homing capability in vivo. Restoration of lymphatic drainage after injection of MSCs in a lymphedema model indicates that MSCs play a role in lymphatic regeneration. The potential clinical application of MSC in wound healing and reduction of lymphatic edema warrants further research.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Conrad</LastName><ForeName>Claudius</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. CConrad1@partners.org</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niess</LastName><ForeName>Hanno</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Huss</LastName><ForeName>Ralf</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Huber</LastName><ForeName>Stephan</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>von Luettichau</LastName><ForeName>Irene</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Nelson</LastName><ForeName>Peter J</ForeName><Initials>PJ</Initials></Author><Author ValidYN="Y"><LastName>Ott</LastName><ForeName>Harald C</ForeName><Initials>HC</Initials></Author><Author ValidYN="Y"><LastName>Jauch</LastName><ForeName>Karl-Walter</ForeName><Initials>KW</Initials></Author><Author ValidYN="Y"><LastName>Bruns</LastName><ForeName>Christiane J</ForeName><Initials>CJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>12</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Circulation</MedlineTA><NlmUniqueID>0147763</NlmUniqueID><ISSNLinking>0009-7322</ISSNLinking></MedlineJournalInfo><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002465" MajorTopicYN="N">Cell Movement</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004729" MajorTopicYN="N">Endothelium, Lymphatic</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042583" MajorTopicYN="N">Lymphangiogenesis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042601" MajorTopicYN="N">Lymphatic Vessels</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059630" MajorTopicYN="N">Mesenchymal Stromal Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D039902" MajorTopicYN="N">Multipotent Stem Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="Y">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012038" MajorTopicYN="N">Regeneration</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>1</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>2</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19118255</ArticleId><ArticleId IdType="pii">CIRCULATIONAHA.108.793208</ArticleId><ArticleId IdType="doi">10.1161/CIRCULATIONAHA.108.793208</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Bruns, Christiane J" sort="Bruns, Christiane J" uniqKey="Bruns C" first="Christiane J" last="Bruns">Christiane J. Bruns</name><name sortKey="Huber, Stephan" sort="Huber, Stephan" uniqKey="Huber S" first="Stephan" last="Huber">Stephan Huber</name><name sortKey="Huss, Ralf" sort="Huss, Ralf" uniqKey="Huss R" first="Ralf" last="Huss">Ralf Huss</name><name sortKey="Jauch, Karl Walter" sort="Jauch, Karl Walter" uniqKey="Jauch K" first="Karl-Walter" last="Jauch">Karl-Walter Jauch</name><name sortKey="Nelson, Peter J" sort="Nelson, Peter J" uniqKey="Nelson P" first="Peter J" last="Nelson">Peter J. Nelson</name><name sortKey="Niess, Hanno" sort="Niess, Hanno" uniqKey="Niess H" first="Hanno" last="Niess">Hanno Niess</name><name sortKey="Ott, Harald C" sort="Ott, Harald C" uniqKey="Ott H" first="Harald C" last="Ott">Harald C. Ott</name><name sortKey="Von Luettichau, Irene" sort="Von Luettichau, Irene" uniqKey="Von Luettichau I" first="Irene" last="Von Luettichau">Irene Von Luettichau</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Conrad, Claudius" sort="Conrad, Claudius" uniqKey="Conrad C" first="Claudius" last="Conrad">Claudius Conrad</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002B62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:19118255
|texte= Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:19118255" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |