Assessment of plasma anti-elastin antibodies for use as a diagnostic aid for chronic progressive lymphoedema in Belgian Draught Horses.
Identifieur interne : 001070 ( PubMed/Checkpoint ); précédent : 001069; suivant : 001071Assessment of plasma anti-elastin antibodies for use as a diagnostic aid for chronic progressive lymphoedema in Belgian Draught Horses.
Auteurs : K. De Keyser [Belgique] ; M. Berth [Belgique] ; N. Christensen [Belgique] ; S. Willaert [Belgique] ; S. Janssens [Belgique] ; R. Ducatelle [Belgique] ; B M Goddeeris [Belgique] ; H E V. De Cock [Belgique] ; N. Buys [Belgique]Source :
- Veterinary immunology and immunopathology [ 1873-2534 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Anticorps (immunologie), Anticorps (sang), Equus caballus (immunologie), Equus caballus (sang), Facteurs de l'âge, Femelle, Grossesse, Lymphoedème (diagnostic), Lymphoedème (immunologie), Lymphoedème (médecine vétérinaire), Lymphoedème (sang), Maladies des chevaux (diagnostic), Maladies des chevaux (immunologie), Maladies des chevaux (sang), Mâle, Reproductibilité des résultats, Sensibilité et spécificité, Test ELISA (médecine vétérinaire), Élastine (immunologie).
- MESH :
- diagnostic : Lymphoedème, Maladies des chevaux.
- immunologie : Anticorps, Equus caballus, Lymphoedème, Maladies des chevaux, Élastine.
- médecine vétérinaire : Lymphoedème, Test ELISA.
- sang : Anticorps, Equus caballus, Lymphoedème, Maladies des chevaux.
- Animaux, Facteurs de l'âge, Femelle, Grossesse, Mâle, Reproductibilité des résultats, Sensibilité et spécificité.
English descriptors
- KwdEn :
- Age Factors, Animals, Antibodies (blood), Antibodies (immunology), Elastin (immunology), Enzyme-Linked Immunosorbent Assay (veterinary), Female, Horse Diseases (blood), Horse Diseases (diagnosis), Horse Diseases (immunology), Horses (blood), Horses (immunology), Lymphedema (blood), Lymphedema (diagnosis), Lymphedema (immunology), Lymphedema (veterinary), Male, Pregnancy, Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- chemical , blood : Antibodies.
- chemical , immunology : Antibodies, Elastin.
- blood : Horse Diseases, Horses, Lymphedema.
- diagnosis : Horse Diseases, Lymphedema.
- immunology : Horse Diseases, Horses, Lymphedema.
- veterinary : Enzyme-Linked Immunosorbent Assay, Lymphedema.
- Age Factors, Animals, Female, Male, Pregnancy, Reproducibility of Results, Sensitivity and Specificity.
Abstract
Diagnosis of chronic progressive lymphoedema (CPL) in draught horses, including the Belgian Draught Horse, is mainly based on clinical evaluation of typical lower limb lesions. A deficient perilymphatic elastic support, caused by a pathological elastin degradation in skin and subcutis, has been suggested as a contributing factor for CPL. Elastin degradation products induce the generation of anti-elastin Ab (AEAb), detectable in horse serum by ELISA. For a clinically healthy group of draught horses, a significantly lower average AEAb-level than 3 clinically affected groups (mild, moderate and severe symptoms) was demonstrated previously. To improve CPL-diagnosis, we evaluated the AEAb-ELISA as an in vitro diagnostic aid in individual horses. Test reproducibility was assessed, performing assays independently in 2 laboratories on a total of 345 horses. Possible factors associated with AEAb-levels (age, gender, pregnancy, test lab and date of blood collection) were analyzed using a mixed statistical model. Results were reproducible in both laboratories. AEAb-levels in moderately and severely affected horses were significantly higher than in healthy horses. Nevertheless, this was only demonstrated in barren mares, and, there was a very large overlap between the clinical groups. Consequently, even when a high AEAb cut-off was handled to obtain a reasonable specificity of 90%, a very low sensitivity (21%) of AEAb for CPL-diagnosis was obtained. Results on the present sample demonstrate that the described ELISA procedure is of no use as a diagnostic test for CPL in individual horses.
DOI: 10.1016/j.vetimm.2014.11.004
PubMed: 25500056
Affiliations:
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<front><div type="abstract" xml:lang="en">Diagnosis of chronic progressive lymphoedema (CPL) in draught horses, including the Belgian Draught Horse, is mainly based on clinical evaluation of typical lower limb lesions. A deficient perilymphatic elastic support, caused by a pathological elastin degradation in skin and subcutis, has been suggested as a contributing factor for CPL. Elastin degradation products induce the generation of anti-elastin Ab (AEAb), detectable in horse serum by ELISA. For a clinically healthy group of draught horses, a significantly lower average AEAb-level than 3 clinically affected groups (mild, moderate and severe symptoms) was demonstrated previously. To improve CPL-diagnosis, we evaluated the AEAb-ELISA as an in vitro diagnostic aid in individual horses. Test reproducibility was assessed, performing assays independently in 2 laboratories on a total of 345 horses. Possible factors associated with AEAb-levels (age, gender, pregnancy, test lab and date of blood collection) were analyzed using a mixed statistical model. Results were reproducible in both laboratories. AEAb-levels in moderately and severely affected horses were significantly higher than in healthy horses. Nevertheless, this was only demonstrated in barren mares, and, there was a very large overlap between the clinical groups. Consequently, even when a high AEAb cut-off was handled to obtain a reasonable specificity of 90%, a very low sensitivity (21%) of AEAb for CPL-diagnosis was obtained. Results on the present sample demonstrate that the described ELISA procedure is of no use as a diagnostic test for CPL in individual horses.</div>
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<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.vetimm.2014.11.004</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S0165-2427(14)00261-X</ELocationID>
<Abstract><AbstractText>Diagnosis of chronic progressive lymphoedema (CPL) in draught horses, including the Belgian Draught Horse, is mainly based on clinical evaluation of typical lower limb lesions. A deficient perilymphatic elastic support, caused by a pathological elastin degradation in skin and subcutis, has been suggested as a contributing factor for CPL. Elastin degradation products induce the generation of anti-elastin Ab (AEAb), detectable in horse serum by ELISA. For a clinically healthy group of draught horses, a significantly lower average AEAb-level than 3 clinically affected groups (mild, moderate and severe symptoms) was demonstrated previously. To improve CPL-diagnosis, we evaluated the AEAb-ELISA as an in vitro diagnostic aid in individual horses. Test reproducibility was assessed, performing assays independently in 2 laboratories on a total of 345 horses. Possible factors associated with AEAb-levels (age, gender, pregnancy, test lab and date of blood collection) were analyzed using a mixed statistical model. Results were reproducible in both laboratories. AEAb-levels in moderately and severely affected horses were significantly higher than in healthy horses. Nevertheless, this was only demonstrated in barren mares, and, there was a very large overlap between the clinical groups. Consequently, even when a high AEAb cut-off was handled to obtain a reasonable specificity of 90%, a very low sensitivity (21%) of AEAb for CPL-diagnosis was obtained. Results on the present sample demonstrate that the described ELISA procedure is of no use as a diagnostic test for CPL in individual horses.</AbstractText>
<CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De Keyser</LastName>
<ForeName>K</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>KU Leuven Department of Biosystems, Division of Gene Technology, Kasteelpark Arenberg 30, 3001 Leuven, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Berth</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Algemeen Medisch Laboratorium, Emiel Vloorsstraat 9, 2020 Hoboken, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Christensen</LastName>
<ForeName>N</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Algemeen Medisch Laboratorium, Emiel Vloorsstraat 9, 2020 Hoboken, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Willaert</LastName>
<ForeName>S</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Algemeen Medisch Laboratorium, Emiel Vloorsstraat 9, 2020 Hoboken, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Janssens</LastName>
<ForeName>S</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>KU Leuven Department of Biosystems, Division of Gene Technology, Kasteelpark Arenberg 30, 3001 Leuven, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ducatelle</LastName>
<ForeName>R</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Ghent University Department of Pathology, Bacteriology and Poultry Diseases, Salisburylaan 133, 9820 Merelbeke, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Goddeeris</LastName>
<ForeName>B M</ForeName>
<Initials>BM</Initials>
<AffiliationInfo><Affiliation>KU Leuven Department of Biosystems, Division of Gene Technology, Kasteelpark Arenberg 30, 3001 Leuven, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>De Cock</LastName>
<ForeName>H E V</ForeName>
<Initials>HE</Initials>
<AffiliationInfo><Affiliation>Algemeen Medisch Laboratorium, Emiel Vloorsstraat 9, 2020 Hoboken, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Buys</LastName>
<ForeName>N</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>KU Leuven Department of Biosystems, Division of Gene Technology, Kasteelpark Arenberg 30, 3001 Leuven, Belgium. Electronic address: nadine.buys@biw.kuleuven.be.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>11</Month>
<Day>14</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Vet Immunol Immunopathol</MedlineTA>
<NlmUniqueID>8002006</NlmUniqueID>
<ISSNLinking>0165-2427</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000906">Antibodies</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-58-3</RegistryNumber>
<NameOfSubstance UI="D004549">Elastin</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000367" MajorTopicYN="N">Age Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004549" MajorTopicYN="N">Elastin</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
<QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006734" MajorTopicYN="N">Horse Diseases</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006736" MajorTopicYN="N">Horses</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008209" MajorTopicYN="N">Lymphedema</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011247" MajorTopicYN="N">Pregnancy</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anti-elastin Ab</Keyword>
<Keyword MajorTopicYN="N">Belgian Draught Horse</Keyword>
<Keyword MajorTopicYN="N">Chronic progressive lymphoedema</Keyword>
<Keyword MajorTopicYN="N">ELISA</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
<Month>02</Month>
<Day>12</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2014</Year>
<Month>11</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2014</Year>
<Month>11</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>12</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>12</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2015</Year>
<Month>8</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">25500056</ArticleId>
<ArticleId IdType="pii">S0165-2427(14)00261-X</ArticleId>
<ArticleId IdType="doi">10.1016/j.vetimm.2014.11.004</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Belgique</li>
</country>
<region><li>Province d'Anvers</li>
<li>Province du Brabant flamand</li>
</region>
<settlement><li>Anvers</li>
<li>Heverlee</li>
<li>Louvain</li>
</settlement>
</list>
<tree><country name="Belgique"><region name="Province du Brabant flamand"><name sortKey="De Keyser, K" sort="De Keyser, K" uniqKey="De Keyser K" first="K" last="De Keyser">K. De Keyser</name>
</region>
<name sortKey="Berth, M" sort="Berth, M" uniqKey="Berth M" first="M" last="Berth">M. Berth</name>
<name sortKey="Buys, N" sort="Buys, N" uniqKey="Buys N" first="N" last="Buys">N. Buys</name>
<name sortKey="Christensen, N" sort="Christensen, N" uniqKey="Christensen N" first="N" last="Christensen">N. Christensen</name>
<name sortKey="De Cock, H E V" sort="De Cock, H E V" uniqKey="De Cock H" first="H E V" last="De Cock">H E V. De Cock</name>
<name sortKey="Ducatelle, R" sort="Ducatelle, R" uniqKey="Ducatelle R" first="R" last="Ducatelle">R. Ducatelle</name>
<name sortKey="Goddeeris, B M" sort="Goddeeris, B M" uniqKey="Goddeeris B" first="B M" last="Goddeeris">B M Goddeeris</name>
<name sortKey="Janssens, S" sort="Janssens, S" uniqKey="Janssens S" first="S" last="Janssens">S. Janssens</name>
<name sortKey="Willaert, S" sort="Willaert, S" uniqKey="Willaert S" first="S" last="Willaert">S. Willaert</name>
</country>
</tree>
</affiliations>
</record>
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