An alternative strategy to generate coding sequence of macrophage migration inhibitory factor-2 of Wuchereria bancrofti.
Identifieur interne : 000A81 ( PubMed/Checkpoint ); précédent : 000A80; suivant : 000A82An alternative strategy to generate coding sequence of macrophage migration inhibitory factor-2 of Wuchereria bancrofti.
Auteurs : Nikhil Chauhan ; S L Hoti [Inde]Source :
- The Indian journal of medical research [ 0971-5916 ] ; 2016.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (isolement et purification), Animaux, Exons (génétique), Facteurs inhibiteurs de la migration des macrophages (génétique), Facteurs inhibiteurs de la migration des macrophages (isolement et purification), Filariose lymphatique (diagnostic), Filariose lymphatique (génétique), Filariose lymphatique (parasitologie), Humains, Réaction de polymérisation en chaîne, Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification), Wuchereria bancrofti (pathogénicité).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : ADN complémentaire, Exons, Facteurs inhibiteurs de la migration des macrophages, Filariose lymphatique, Wuchereria bancrofti.
- isolement et purification : ADN complémentaire, Facteurs inhibiteurs de la migration des macrophages, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- pathogénicité : Wuchereria bancrofti.
- Animaux, Humains, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Animals, DNA, Complementary (genetics), DNA, Complementary (isolation & purification), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (genetics), Elephantiasis, Filarial (parasitology), Exons (genetics), Humans, Macrophage Migration-Inhibitory Factors (genetics), Macrophage Migration-Inhibitory Factors (isolation & purification), Polymerase Chain Reaction, Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification), Wuchereria bancrofti (pathogenicity).
- MESH :
- chemical , genetics : DNA, Complementary, Macrophage Migration-Inhibitory Factors.
- chemical , isolation & purification : DNA, Complementary, Macrophage Migration-Inhibitory Factors.
- diagnosis : Elephantiasis, Filarial.
- genetics : Elephantiasis, Filarial, Exons, Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- pathogenicity : Wuchereria bancrofti.
- Animals, Humans, Polymerase Chain Reaction.
Abstract
Different developmental stages of Wuchereria bancrofti, the major causal organism of lymphatic filariasis (LF), are difficult to obtain. Beside this limitation, to obtain complete coding sequence (CDS) of a gene one has to isolate mRNA and perform subsequent cDNA synthesis which is laborious and not successful at times. In this study, an alternative strategy employing polymerase chain reaction (PCR) was optimized and validated, to generate CDS of Macrophage migration Inhibitory Factor-2 (wbMIF-2), a gene expressed in the transition stage between L3 to L4.
DOI: 10.4103/0971-5916.180217
PubMed: 27121522
Affiliations:
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pubmed:27121522Le document en format XML
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Scientist 'G' and Director-in-Charge, Regional Medical Research Centre (ICMR), Nehru Nagar, Belagavi 590 010, Karnataka, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Microbiology & Immunology, Vector Control Research Centre (ICMR), Puducherry; Scientist 'G' and Director-in-Charge, Regional Medical Research Centre (ICMR), Nehru Nagar, Belagavi 590 010, Karnataka</wicri:regionArea><wicri:noRegion>Karnataka</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Indian journal of medical research</title><idno type="ISSN">0971-5916</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (isolation & purification)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (genetics)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Exons (genetics)</term><term>Humans</term><term>Macrophage Migration-Inhibitory Factors (genetics)</term><term>Macrophage Migration-Inhibitory Factors (isolation & purification)</term><term>Polymerase Chain Reaction</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term><term>Wuchereria bancrofti (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (isolement et purification)</term><term>Animaux</term><term>Exons (génétique)</term><term>Facteurs inhibiteurs de la migration des macrophages (génétique)</term><term>Facteurs inhibiteurs de la migration des macrophages (isolement et purification)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (génétique)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (isolement et purification)</term><term>Wuchereria bancrofti (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Macrophage Migration-Inhibitory Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term><term>Macrophage Migration-Inhibitory Factors</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Exons</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Exons</term><term>Facteurs inhibiteurs de la migration des macrophages</term><term>Filariose lymphatique</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>Facteurs inhibiteurs de la migration des macrophages</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Different developmental stages of Wuchereria bancrofti, the major causal organism of lymphatic filariasis (LF), are difficult to obtain. Beside this limitation, to obtain complete coding sequence (CDS) of a gene one has to isolate mRNA and perform subsequent cDNA synthesis which is laborious and not successful at times. In this study, an alternative strategy employing polymerase chain reaction (PCR) was optimized and validated, to generate CDS of Macrophage migration Inhibitory Factor-2 (wbMIF-2), a gene expressed in the transition stage between L3 to L4.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27121522</PMID><DateCreated><Year>2016</Year><Month>04</Month><Day>28</Day></DateCreated><DateCompleted><Year>2017</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2017</Year><Month>08</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0971-5916</ISSN><JournalIssue CitedMedium="Internet"><Volume>143</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>The Indian journal of medical research</Title><ISOAbbreviation>Indian J. Med. Res.</ISOAbbreviation></Journal><ArticleTitle>An alternative strategy to generate coding sequence of macrophage migration inhibitory factor-2 of Wuchereria bancrofti.</ArticleTitle><Pagination><MedlinePgn>232-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.4103/0971-5916.180217</ELocationID><Abstract><AbstractText Label="BACKGROUND & OBJECTIVES" NlmCategory="OBJECTIVE">Different developmental stages of Wuchereria bancrofti, the major causal organism of lymphatic filariasis (LF), are difficult to obtain. Beside this limitation, to obtain complete coding sequence (CDS) of a gene one has to isolate mRNA and perform subsequent cDNA synthesis which is laborious and not successful at times. In this study, an alternative strategy employing polymerase chain reaction (PCR) was optimized and validated, to generate CDS of Macrophage migration Inhibitory Factor-2 (wbMIF-2), a gene expressed in the transition stage between L3 to L4.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The genomic DNA of W. bancrofti microfilariae was extracted and used to amplify the full length wbMIF-2 gene (4.275 kb). This amplified product was used as a template for amplifying the exons separately, using the overlapping primers, which were then assembled through another round of PCR.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">A simple strategy was developed based on PCR, which is used routinely in molecular biology laboratories. The amplified CDS of 363 bp of wbMIF-2 generated using genomic DNA splicing technique was devoid of any intronic sequence.</AbstractText><AbstractText Label="INTERPRETATION & CONCLUSIONS" NlmCategory="CONCLUSIONS">The cDNA of wbMIF-2 gene was successfully amplified from genomic DNA of microfilarial stage of W. bancrofti thus circumventing the use of inaccessible L3-L4 transitional stage of this parasite. This strategy is useful for generating CDS of genes from parasites that have restricted availability.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chauhan</LastName><ForeName>Nikhil</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Hoti</LastName><ForeName>S L</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>Division of Microbiology & Immunology, Vector Control Research Centre (ICMR), Puducherry; Scientist 'G' and Director-in-Charge, Regional Medical Research Centre (ICMR), Nehru Nagar, Belagavi 590 010, Karnataka, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>India</Country><MedlineTA>Indian J Med Res</MedlineTA><NlmUniqueID>0374701</NlmUniqueID><ISSNLinking>0971-5916</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008263">Macrophage Migration-Inhibitory Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 2002 Feb;70(2):427-33</RefSource><PMID Version="1">11796567</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biomol Tech. 2004 Sep;15(3):155-66</RefSource><PMID Version="1">15331581</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Parasitol. 1970 Jun;56(3):623-4</RefSource><PMID Version="1">5421099</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Trop. 1974;31(2):108-28</RefSource><PMID Version="1">4152907</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Southeast Asian J Trop Med Public Health. 1975 Sep;6(3):328-37</RefSource><PMID Version="1">1221502</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Trends Parasitol. 2005 Apr;21(4):162-8</RefSource><PMID Version="1">15780837</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Protoc. 2006;1(2):791-7</RefSource><PMID Version="1">17406309</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2007;2(11):e1179</RefSource><PMID Version="1">18000550</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biotechniques. 2007 Dec;43(6):785-9</RefSource><PMID Version="1">18251255</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biochem Parasitol. 2008 Jul;160(1):8-21</RefSource><PMID Version="1">18439691</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biotechnol. 2008 Jul;39(3):201-6</RefSource><PMID Version="1">18228164</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Negl Trop Dis. 2009;3(4):e410</RefSource><PMID Version="1">19352421</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Biol Evol. 2011 Oct;28(10):2731-9</RefSource><PMID Version="1">21546353</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genet Mol Res. 2011;10(4):3455-62</RefSource><PMID Version="1">22180065</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Wkly Epidemiol Rec. 2013 Sep 13;88(37):389-99</RefSource><PMID Version="1">24073461</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Negl Trop Dis. 2014 Nov;8(11):e3319</RefSource><PMID Version="1">25412180</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005091" MajorTopicYN="N">Exons</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008263" MajorTopicYN="N">Macrophage Migration-Inhibitory Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4859133</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27121522</ArticleId><ArticleId IdType="pii">IndianJMedRes_2016_143_2_232_180217</ArticleId><ArticleId IdType="doi">10.4103/0971-5916.180217</ArticleId><ArticleId IdType="pmc">PMC4859133</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Chauhan, Nikhil" sort="Chauhan, Nikhil" uniqKey="Chauhan N" first="Nikhil" last="Chauhan">Nikhil Chauhan</name></noCountry><country name="Inde"><noRegion><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document 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