LymphedemaV1, PubMed, Checkpoint, bibRecord, 000834

Local inhibition of elastase reduces EMILIN1 cleavage reactivating lymphatic vessel function in a mouse lymphoedema model.

Identifieur interne : 000834 ( PubMed/Checkpoint ); précédent : 000833; suivant : 000835

Local inhibition of elastase reduces EMILIN1 cleavage reactivating lymphatic vessel function in a mouse lymphoedema model.

Auteurs : Eliana Pivetta [Italie] ; Bruna Wassermann [Italie] ; Lisa Del Bel Belluz [Suède] ; Carla Danussi [États-Unis] ; Teresa Maria Elisa Modica [Italie] ; Orlando Maiorani [Italie] ; Giulia Bosisio [Italie] ; Francesco Boccardo [Italie] ; Vincenzo Canzonieri [Italie] ; Alfonso Colombatti [Italie] ; Paola Spessotto [Italie]

Source :

Clinical science (London, England : 1979) [ 1470-8736 ] ; 2016.

RBID : pubmed:26920215

Descripteurs français

KwdFr :
- Animaux, Cellules cultivées, Cellules endothéliales (physiologie), Femelle, Glycoprotéines membranaires (physiologie), Humains, Infiltration par les neutrophiles, Lymphoedème (métabolisme), Lymphoedème (traitement médicamenteux), Modèles animaux de maladie humaine, Protéines sécrétoires inhibitrices de protéinases (pharmacologie), Protéines sécrétoires inhibitrices de protéinases (usage thérapeutique), Souris, Souris de lignée C57BL, Vaisseaux lymphatiques (), Vaisseaux lymphatiques (physiologie).
MESH :
- métabolisme : Lymphoedème.
- pharmacologie : Protéines sécrétoires inhibitrices de protéinases.
- physiologie : Cellules endothéliales, Glycoprotéines membranaires, Vaisseaux lymphatiques.
- traitement médicamenteux : Lymphoedème.
- usage thérapeutique : Protéines sécrétoires inhibitrices de protéinases.
- Animaux, Cellules cultivées, Femelle, Humains, Infiltration par les neutrophiles, Modèles animaux de maladie humaine, Souris, Souris de lignée C57BL, Vaisseaux lymphatiques.

English descriptors

KwdEn :
- Animals, Cells, Cultured, Disease Models, Animal, Endothelial Cells (physiology), Female, Humans, Lymphatic Vessels (drug effects), Lymphatic Vessels (physiology), Lymphedema (drug therapy), Lymphedema (metabolism), Membrane Glycoproteins (physiology), Mice, Mice, Inbred C57BL, Neutrophil Infiltration, Proteinase Inhibitory Proteins, Secretory (pharmacology), Proteinase Inhibitory Proteins, Secretory (therapeutic use).
MESH :
- chemical , pharmacology : Proteinase Inhibitory Proteins, Secretory.
- chemical , physiology : Membrane Glycoproteins.
- drug effects : Lymphatic Vessels.
- drug therapy : Lymphedema.
- metabolism : Lymphedema.
- physiology : Endothelial Cells, Lymphatic Vessels.
- chemical , therapeutic use : Proteinase Inhibitory Proteins, Secretory.
- Animals, Cells, Cultured, Disease Models, Animal, Female, Humans, Mice, Mice, Inbred C57BL, Neutrophil Infiltration.

Abstract

Lymphatic vasculature critically depends on the connections of lymphatic endothelial cells with the extracellular matrix (ECM), which are mediated by anchoring filaments (AFs). The ECM protein EMILIN1 is a component of AFs and is involved in the regulation of lymphatic vessel functions: accordingly, Emilin1(-/-) mice display lymphatic vascular morphological alterations, leading to functional defects such as mild lymphoedema, lymph leakage and compromised lymph drainage. In the present study, using a mouse post-surgical tail lymphoedema model, we show that the acute phase of acquired lymphoedema correlates with EMILIN1 degradation due to neutrophil elastase (NE) released by infiltrating neutrophils. As a consequence, the intercellular junctions of lymphatic endothelial cells are weakened and drainage to regional lymph nodes is severely affected. The local administration of sivelestat, a specific NE inhibitor, prevents EMILIN1 degradation and reduces lymphoedema, restoring a normal lymphatic functionality. The finding that, in human secondary lymphoedema samples, we also detected cleaved EMILIN1 with the typical bands of an NE-dependent pattern of fragmentation establishes a rationale for a powerful strategy that targets NE inhibition. In conclusion, the attempts to block EMILIN1 degradation locally represent the basis for a novel 'ECM' pharmacological approach to assessing new lymphoedema treatments.

DOI: 10.1042/CS20160064
PubMed: 26920215

Affiliations:

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Le document en format XML

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<term>Lymphedema (drug therapy)</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Endothelial Cells</term>
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<term>Infiltration par les neutrophiles</term>
<term>Modèles animaux de maladie humaine</term>
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<front><div type="abstract" xml:lang="en">Lymphatic vasculature critically depends on the connections of lymphatic endothelial cells with the extracellular matrix (ECM), which are mediated by anchoring filaments (AFs). The ECM protein EMILIN1 is a component of AFs and is involved in the regulation of lymphatic vessel functions: accordingly, Emilin1(-/-) mice display lymphatic vascular morphological alterations, leading to functional defects such as mild lymphoedema, lymph leakage and compromised lymph drainage. In the present study, using a mouse post-surgical tail lymphoedema model, we show that the acute phase of acquired lymphoedema correlates with EMILIN1 degradation due to neutrophil elastase (NE) released by infiltrating neutrophils. As a consequence, the intercellular junctions of lymphatic endothelial cells are weakened and drainage to regional lymph nodes is severely affected. The local administration of sivelestat, a specific NE inhibitor, prevents EMILIN1 degradation and reduces lymphoedema, restoring a normal lymphatic functionality. The finding that, in human secondary lymphoedema samples, we also detected cleaved EMILIN1 with the typical bands of an NE-dependent pattern of fragmentation establishes a rationale for a powerful strategy that targets NE inhibition. In conclusion, the attempts to block EMILIN1 degradation locally represent the basis for a novel 'ECM' pharmacological approach to assessing new lymphoedema treatments.</div>
</front>
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<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1470-8736</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>130</Volume>
<Issue>14</Issue>
<PubDate><Year>2016</Year>
<Month>Jul</Month>
<Day>01</Day>
</PubDate>
</JournalIssue>
<Title>Clinical science (London, England : 1979)</Title>
<ISOAbbreviation>Clin. Sci.</ISOAbbreviation>
</Journal>
<ArticleTitle>Local inhibition of elastase reduces EMILIN1 cleavage reactivating lymphatic vessel function in a mouse lymphoedema model.</ArticleTitle>
<Pagination><MedlinePgn>1221-36</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1042/CS20160064</ELocationID>
<Abstract><AbstractText>Lymphatic vasculature critically depends on the connections of lymphatic endothelial cells with the extracellular matrix (ECM), which are mediated by anchoring filaments (AFs). The ECM protein EMILIN1 is a component of AFs and is involved in the regulation of lymphatic vessel functions: accordingly, Emilin1(-/-) mice display lymphatic vascular morphological alterations, leading to functional defects such as mild lymphoedema, lymph leakage and compromised lymph drainage. In the present study, using a mouse post-surgical tail lymphoedema model, we show that the acute phase of acquired lymphoedema correlates with EMILIN1 degradation due to neutrophil elastase (NE) released by infiltrating neutrophils. As a consequence, the intercellular junctions of lymphatic endothelial cells are weakened and drainage to regional lymph nodes is severely affected. The local administration of sivelestat, a specific NE inhibitor, prevents EMILIN1 degradation and reduces lymphoedema, restoring a normal lymphatic functionality. The finding that, in human secondary lymphoedema samples, we also detected cleaved EMILIN1 with the typical bands of an NE-dependent pattern of fragmentation establishes a rationale for a powerful strategy that targets NE inhibition. In conclusion, the attempts to block EMILIN1 degradation locally represent the basis for a novel 'ECM' pharmacological approach to assessing new lymphoedema treatments.</AbstractText>
<CopyrightInformation>© 2016 The Author(s).</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pivetta</LastName>
<ForeName>Eliana</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wassermann</LastName>
<ForeName>Bruna</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Del Bel Belluz</LastName>
<ForeName>Lisa</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Danussi</LastName>
<ForeName>Carla</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, U.S.A.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Modica</LastName>
<ForeName>Teresa Maria Elisa</ForeName>
<Initials>TM</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Maiorani</LastName>
<ForeName>Orlando</ForeName>
<Initials>O</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bosisio</LastName>
<ForeName>Giulia</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boccardo</LastName>
<ForeName>Francesco</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Department of Surgery-Unit of Lymphatic Surgery, IRCCS S. Martino University Hospital-IST, National Institute for Cancer Research, University of Genoa, 16132 Genoa, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Canzonieri</LastName>
<ForeName>Vincenzo</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>Division of Pathology, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Colombatti</LastName>
<ForeName>Alfonso</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Spessotto</LastName>
<ForeName>Paola</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Experimental Oncology 2, Department of Translational Research, CRO-IRCCS, National Cancer Institute, 33081, Aviano, Italy pspessotto@cro.it.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>02</Month>
<Day>26</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Clin Sci (Lond)</MedlineTA>
<NlmUniqueID>7905731</NlmUniqueID>
<ISSNLinking>0143-5221</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D053491">Proteinase Inhibitory Proteins, Secretory</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C030340">elastin microfibril interface located protein</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Am J Physiol Cell Physiol. 2012 Jan 15;302(2):C392-404</RefSource>
<PMID Version="1">21940662</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Leukoc Biol. 2015 Dec;98(6):897-912</RefSource>
<PMID Version="1">26216937</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>World J Gastroenterol. 2015 Mar 28;21(12):3720-30</RefSource>
<PMID Version="1">25834341</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Am J Physiol Heart Circ Physiol. 2006 Sep;291(3):H1402-10</RefSource>
<PMID Version="1">16648194</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Ann N Y Acad Sci. 2002 Dec;979:94-110</RefSource>
<PMID Version="1">12543720</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Int Angiol. 1999 Mar;18(1):31-41</RefSource>
<PMID Version="1">10392478</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Mol Cell Biol. 2013 Nov;33(22):4381-94</RefSource>
<PMID Version="1">24019067</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Anal Biochem. 2012 Jun 15;425(2):96-103</RefSource>
<PMID Version="1">22449498</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Surg Res. 2015 Jun 1;196(1):200-7</RefSource>
<PMID Version="1">25777822</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>FASEB J. 2007 Apr;21(4):1003-12</RefSource>
<PMID Version="1">17210781</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Rev Immunol. 2006 Mar;6(3):173-82</RefSource>
<PMID Version="1">16498448</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Cell Biol. 1985 Jan;100(1):18-26</RefSource>
<PMID Version="1">3880750</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Curr Treat Options Cardiovasc Med. 2012 Apr;14(2):184-92</RefSource>
<PMID Version="1">22382848</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Intensive Care. 2014 Dec 31;2(1):67</RefSource>
<PMID Version="1">25705423</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nature. 2005 Dec 15;438(7070):946-53</RefSource>
<PMID Version="1">16355212</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2000 Aug 18;275(33):25471-80</RefSource>
<PMID Version="1">10821830</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Lymphat Res Biol. 2009;7(1):47-57</RefSource>
<PMID Version="1">19302023</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Microvasc Res. 2006 Nov;72(3):161-71</RefSource>
<PMID Version="1">16876204</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Int J Biol Macromol. 2012 Oct;51(3):196-200</RefSource>
<PMID Version="1">22579959</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Mol Cell Biol. 2008 Jun;28(12):4026-39</RefSource>
<PMID Version="1">18411305</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2003 Feb 21;278(8):6160-7</RefSource>
<PMID Version="1">12456677</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Eur J Cancer Care (Engl). 1995 Mar;4(1):11-6</RefSource>
<PMID Version="1">7620649</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Cell. 2006 Mar 10;124(5):929-42</RefSource>
<PMID Version="1">16530041</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Am J Pathol. 2010 Dec;177(6):3202-14</RefSource>
<PMID Version="1">21056998</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Matrix Biol. 2014 Feb;34:22-32</RefSource>
<PMID Version="1">24513040</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Mol Cell Biol. 2004 Jan;24(2):638-50</RefSource>
<PMID Version="1">14701737</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Ann N Y Acad Sci. 2008;1131:50-74</RefSource>
<PMID Version="1">18519959</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Med. 2007 Dec;13(12):1458-66</RefSource>
<PMID Version="1">18059280</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Curr Opin Hematol. 2014 Jan;21(1):23-8</RefSource>
<PMID Version="1">24241342</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>PLoS Med. 2006 Jul;3(7):e254</RefSource>
<PMID Version="1">16834456</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Angiogenesis. 2014 Apr;17(2):325-34</RefSource>
<PMID Version="1">24449090</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Microsc Res Tech. 2001 Oct 15;55(2):122-45</RefSource>
<PMID Version="1">11596157</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2009 Dec 21;4(12):e8380</RefSource>
<PMID Version="1">20027220</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Lymphat Res Biol. 2006;4(2):67-72</RefSource>
<PMID Version="1">16808668</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Circ Res. 2005 Jun 10;96(11):1193-9</RefSource>
<PMID Version="1">15890974</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Biomech. 1999 Dec;32(12 ):1297-307</RefSource>
<PMID Version="1">10569708</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Ann Plast Surg. 2013 Aug;71(2):191-5</RefSource>
<PMID Version="1">23542829</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Coll Relat Res. 1987 Sep;7(4):259-75</RefSource>
<PMID Version="1">3311601</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Mol Oncol. 2013 Apr;7(2):259-82</RefSource>
<PMID Version="1">23522958</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Oncol Lett. 2015 Apr;9(4):1889-1892</RefSource>
<PMID Version="1">25789062</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Exp Cell Res. 1999 Feb 1;246(2):368-75</RefSource>
<PMID Version="1">9925752</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Chem Res Toxicol. 2005 Feb;18(2):154-61</RefSource>
<PMID Version="1">15720119</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Cell Biol. 2011 Oct 3;195(1):131-45</RefSource>
<PMID Version="1">21949412</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Cochrane Database Syst Rev. 2004;(2):CD003140</RefSource>
<PMID Version="1">15106192</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Cancer Prev Res (Phila). 2012 Sep;5(9):1131-43</RefSource>
<PMID Version="1">22827975</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Clin Invest. 2014 Mar;124(3):936-42</RefSource>
<PMID Version="1">24590279</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Lymphology. 1991 Sep;24(3):105-12</RefSource>
<PMID Version="1">1753801</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042783" MajorTopicYN="N">Endothelial Cells</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042601" MajorTopicYN="N">Lymphatic Vessels</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008209" MajorTopicYN="N">Lymphedema</DescriptorName>
<QualifierName UI="Q000188" MajorTopicYN="Y">drug therapy</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020556" MajorTopicYN="N">Neutrophil Infiltration</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053491" MajorTopicYN="N">Proteinase Inhibitory Proteins, Secretory</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
<QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName>
</MeshHeading>
</MeshHeadingList>
<OtherID Source="NLM">PMC4888021</OtherID>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">EMILIN1</Keyword>
<Keyword MajorTopicYN="N">extracellular matrix</Keyword>
<Keyword MajorTopicYN="N">human lymphoedematous tissue</Keyword>
<Keyword MajorTopicYN="N">neutrophil elastase</Keyword>
<Keyword MajorTopicYN="N">secondary lymphoedema</Keyword>
<Keyword MajorTopicYN="N">sivelestat</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>09</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>02</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>6</Hour>
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<Hour>6</Hour>
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<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>6</Month>
<Day>22</Day>
<Hour>6</Hour>
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</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">26920215</ArticleId>
<ArticleId IdType="pii">CS20160064</ArticleId>
<ArticleId IdType="doi">10.1042/CS20160064</ArticleId>
<ArticleId IdType="pmc">PMC4888021</ArticleId>
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</pubmed>
<affiliations><list><country><li>Italie</li>
<li>Suède</li>
<li>États-Unis</li>
</country>
<region><li>État de New York</li>
</region>
</list>
<tree><country name="Italie"><noRegion><name sortKey="Pivetta, Eliana" sort="Pivetta, Eliana" uniqKey="Pivetta E" first="Eliana" last="Pivetta">Eliana Pivetta</name>
</noRegion>
<name sortKey="Boccardo, Francesco" sort="Boccardo, Francesco" uniqKey="Boccardo F" first="Francesco" last="Boccardo">Francesco Boccardo</name>
<name sortKey="Bosisio, Giulia" sort="Bosisio, Giulia" uniqKey="Bosisio G" first="Giulia" last="Bosisio">Giulia Bosisio</name>
<name sortKey="Canzonieri, Vincenzo" sort="Canzonieri, Vincenzo" uniqKey="Canzonieri V" first="Vincenzo" last="Canzonieri">Vincenzo Canzonieri</name>
<name sortKey="Colombatti, Alfonso" sort="Colombatti, Alfonso" uniqKey="Colombatti A" first="Alfonso" last="Colombatti">Alfonso Colombatti</name>
<name sortKey="Maiorani, Orlando" sort="Maiorani, Orlando" uniqKey="Maiorani O" first="Orlando" last="Maiorani">Orlando Maiorani</name>
<name sortKey="Modica, Teresa Maria Elisa" sort="Modica, Teresa Maria Elisa" uniqKey="Modica T" first="Teresa Maria Elisa" last="Modica">Teresa Maria Elisa Modica</name>
<name sortKey="Spessotto, Paola" sort="Spessotto, Paola" uniqKey="Spessotto P" first="Paola" last="Spessotto">Paola Spessotto</name>
<name sortKey="Wassermann, Bruna" sort="Wassermann, Bruna" uniqKey="Wassermann B" first="Bruna" last="Wassermann">Bruna Wassermann</name>
</country>
<country name="Suède"><noRegion><name sortKey="Del Bel Belluz, Lisa" sort="Del Bel Belluz, Lisa" uniqKey="Del Bel Belluz L" first="Lisa" last="Del Bel Belluz">Lisa Del Bel Belluz</name>
</noRegion>
</country>
<country name="États-Unis"><region name="État de New York"><name sortKey="Danussi, Carla" sort="Danussi, Carla" uniqKey="Danussi C" first="Carla" last="Danussi">Carla Danussi</name>
</region>
</country>
</tree>
</affiliations>
</record>

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Local inhibition of elastase reduces EMILIN1 cleavage reactivating lymphatic vessel function in a mouse lymphoedema model.

Local inhibition of elastase reduces EMILIN1 cleavage reactivating lymphatic vessel function in a mouse lymphoedema model.

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