Colorimetric tests for diagnosis of filarial infection and vector surveillance using non-instrumented nucleic acid loop-mediated isothermal amplification (NINA-LAMP).
Identifieur interne : 000428 ( PubMed/Checkpoint ); précédent : 000427; suivant : 000429Colorimetric tests for diagnosis of filarial infection and vector surveillance using non-instrumented nucleic acid loop-mediated isothermal amplification (NINA-LAMP).
Auteurs : Catherine B. Poole ; Zhiru Li ; Andy Alhassan ; Dylan Guelig [États-Unis] ; Steven Diesburg [États-Unis] ; Nathan A. Tanner ; Yinhua Zhang ; Thomas C. Evans ; Paul Labarre [États-Unis] ; Samuel Wanji [Cameroun] ; Robert A. Burton [États-Unis] ; Clotilde K S. CarlowSource :
- PloS one [ 1932-6203 ] ; 2017.
Descripteurs français
- KwdFr :
- ADN des helminthes (génétique), Aedes (parasitologie), Animaux, Colorimétrie, Filariose lymphatique (diagnostic), Filariose lymphatique (génétique), Humains, Onchocerca volvulus (génétique), Onchocercose (diagnostic), Onchocercose (génétique), Simuliidae (parasitologie), Techniques d'amplification d'acides nucléiques (), Wuchereria bancrofti (génétique).
- MESH :
- diagnostic : Filariose lymphatique, Onchocercose.
- génétique : ADN des helminthes, Filariose lymphatique, Onchocerca volvulus, Onchocercose, Wuchereria bancrofti.
- parasitologie : Aedes, Simuliidae.
- Animaux, Colorimétrie, Humains, Techniques d'amplification d'acides nucléiques.
English descriptors
- KwdEn :
- Aedes (parasitology), Animals, Colorimetry, DNA, Helminth (genetics), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (genetics), Humans, Nucleic Acid Amplification Techniques (methods), Onchocerca volvulus (genetics), Onchocerciasis (diagnosis), Onchocerciasis (genetics), Simuliidae (parasitology), Wuchereria bancrofti (genetics).
- MESH :
- chemical , genetics : DNA, Helminth.
- diagnosis : Elephantiasis, Filarial, Onchocerciasis.
- genetics : Elephantiasis, Filarial, Onchocerca volvulus, Onchocerciasis, Wuchereria bancrofti.
- methods : Nucleic Acid Amplification Techniques.
- parasitology : Aedes, Simuliidae.
- Animals, Colorimetry, Humans.
Abstract
Accurate detection of filarial parasites in humans is essential for the implementation and evaluation of mass drug administration programs to control onchocerciasis and lymphatic filariasis. Determining the infection levels in vector populations is also important for assessing transmission, deciding when drug treatments may be terminated and for monitoring recrudescence. Immunological methods to detect infection in humans are available, however, cross-reactivity issues have been reported. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity, and can be used to detect infection in both humans and vectors. In this study we developed loop-mediated isothermal amplification (LAMP) tests to detect three different filarial DNAs in human and insect samples using pH sensitive dyes for enhanced visual detection of amplification. Furthermore, reactions were performed in a portable, non-instrumented nucleic acid amplification (NINA) device that provides a stable heat source for LAMP. The efficacy of several strand displacing DNA polymerases were evaluated in combination with neutral red or phenol red dyes. Colorimetric NINA-LAMP assays targeting Brugia Hha I repeat, Onchocerca volvulus GST1a and Wuchereria bancrofti LDR each exhibit species-specificity and are also highly sensitive, detecting DNA equivalent to 1/10-1/5000th of one microfilaria. Reaction times varied depending on whether a single copy gene (70 minutes, O. volvulus) or repetitive DNA (40 min, B. malayi and W. bancrofti) was employed as a biomarker. The NINA heater can be used to detect multiple infections simultaneously. The accuracy, simplicity and versatility of the technology suggests that colorimetric NINA-LAMP assays are ideally suited for monitoring the success of filariasis control programs.
DOI: 10.1371/journal.pone.0169011
PubMed: 28199317
Affiliations:
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Poole</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Li, Zhiru" sort="Li, Zhiru" uniqKey="Li Z" first="Zhiru" last="Li">Zhiru Li</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Alhassan, Andy" sort="Alhassan, Andy" uniqKey="Alhassan A" first="Andy" last="Alhassan">Andy Alhassan</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Guelig, Dylan" sort="Guelig, Dylan" uniqKey="Guelig D" first="Dylan" last="Guelig">Dylan Guelig</name><affiliation wicri:level="2"><nlm:affiliation>PATH, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>PATH, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Diesburg, Steven" sort="Diesburg, Steven" uniqKey="Diesburg S" first="Steven" last="Diesburg">Steven Diesburg</name><affiliation wicri:level="2"><nlm:affiliation>PATH, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>PATH, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Tanner, Nathan A" sort="Tanner, Nathan A" uniqKey="Tanner N" first="Nathan A" last="Tanner">Nathan A. 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Poole</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Li, Zhiru" sort="Li, Zhiru" uniqKey="Li Z" first="Zhiru" last="Li">Zhiru Li</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Alhassan, Andy" sort="Alhassan, Andy" uniqKey="Alhassan A" first="Andy" last="Alhassan">Andy Alhassan</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Guelig, Dylan" sort="Guelig, Dylan" uniqKey="Guelig D" first="Dylan" last="Guelig">Dylan Guelig</name><affiliation wicri:level="2"><nlm:affiliation>PATH, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>PATH, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Diesburg, Steven" sort="Diesburg, Steven" uniqKey="Diesburg S" first="Steven" last="Diesburg">Steven Diesburg</name><affiliation wicri:level="2"><nlm:affiliation>PATH, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>PATH, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Tanner, Nathan A" sort="Tanner, Nathan A" uniqKey="Tanner N" first="Nathan A" last="Tanner">Nathan A. Tanner</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Yinhua" sort="Zhang, Yinhua" uniqKey="Zhang Y" first="Yinhua" last="Zhang">Yinhua Zhang</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author><author><name sortKey="Evans, Thomas C" sort="Evans, Thomas C" uniqKey="Evans T" first="Thomas C" last="Evans">Thomas C. 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Burton</name><affiliation wicri:level="2"><nlm:affiliation>PATH, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>PATH, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Carlow, Clotilde K S" sort="Carlow, Clotilde K S" uniqKey="Carlow C" first="Clotilde K S" last="Carlow">Clotilde K S. Carlow</name><affiliation><nlm:affiliation>New England Biolabs, Ipswich, MA United States of America.</nlm:affiliation><wicri:noCountry code="subField">MA United States of America</wicri:noCountry></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aedes (parasitology)</term><term>Animals</term><term>Colorimetry</term><term>DNA, Helminth (genetics)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (genetics)</term><term>Humans</term><term>Nucleic Acid Amplification Techniques (methods)</term><term>Onchocerca volvulus (genetics)</term><term>Onchocerciasis (diagnosis)</term><term>Onchocerciasis (genetics)</term><term>Simuliidae (parasitology)</term><term>Wuchereria bancrofti (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (génétique)</term><term>Aedes (parasitologie)</term><term>Animaux</term><term>Colorimétrie</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (génétique)</term><term>Humains</term><term>Onchocerca volvulus (génétique)</term><term>Onchocercose (diagnostic)</term><term>Onchocercose (génétique)</term><term>Simuliidae (parasitologie)</term><term>Techniques d'amplification d'acides nucléiques ()</term><term>Wuchereria bancrofti (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Onchocerciasis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term><term>Onchocercose</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Onchocerca volvulus</term><term>Onchocerciasis</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des helminthes</term><term>Filariose lymphatique</term><term>Onchocerca volvulus</term><term>Onchocercose</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Aedes</term><term>Simuliidae</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Aedes</term><term>Simuliidae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Colorimetry</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Colorimétrie</term><term>Humains</term><term>Techniques d'amplification d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Accurate detection of filarial parasites in humans is essential for the implementation and evaluation of mass drug administration programs to control onchocerciasis and lymphatic filariasis. Determining the infection levels in vector populations is also important for assessing transmission, deciding when drug treatments may be terminated and for monitoring recrudescence. Immunological methods to detect infection in humans are available, however, cross-reactivity issues have been reported. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity, and can be used to detect infection in both humans and vectors. In this study we developed loop-mediated isothermal amplification (LAMP) tests to detect three different filarial DNAs in human and insect samples using pH sensitive dyes for enhanced visual detection of amplification. Furthermore, reactions were performed in a portable, non-instrumented nucleic acid amplification (NINA) device that provides a stable heat source for LAMP. The efficacy of several strand displacing DNA polymerases were evaluated in combination with neutral red or phenol red dyes. Colorimetric NINA-LAMP assays targeting Brugia Hha I repeat, Onchocerca volvulus GST1a and Wuchereria bancrofti LDR each exhibit species-specificity and are also highly sensitive, detecting DNA equivalent to 1/10-1/5000th of one microfilaria. Reaction times varied depending on whether a single copy gene (70 minutes, O. volvulus) or repetitive DNA (40 min, B. malayi and W. bancrofti) was employed as a biomarker. The NINA heater can be used to detect multiple infections simultaneously. The accuracy, simplicity and versatility of the technology suggests that colorimetric NINA-LAMP assays are ideally suited for monitoring the success of filariasis control programs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28199317</PMID><DateCreated><Year>2017</Year><Month>02</Month><Day>15</Day></DateCreated><DateCompleted><Year>2017</Year><Month>08</Month><Day>09</Day></DateCompleted><DateRevised><Year>2017</Year><Month>08</Month><Day>09</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>2</Issue><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Colorimetric tests for diagnosis of filarial infection and vector surveillance using non-instrumented nucleic acid loop-mediated isothermal amplification (NINA-LAMP).</ArticleTitle><Pagination><MedlinePgn>e0169011</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0169011</ELocationID><Abstract><AbstractText>Accurate detection of filarial parasites in humans is essential for the implementation and evaluation of mass drug administration programs to control onchocerciasis and lymphatic filariasis. Determining the infection levels in vector populations is also important for assessing transmission, deciding when drug treatments may be terminated and for monitoring recrudescence. Immunological methods to detect infection in humans are available, however, cross-reactivity issues have been reported. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity, and can be used to detect infection in both humans and vectors. In this study we developed loop-mediated isothermal amplification (LAMP) tests to detect three different filarial DNAs in human and insect samples using pH sensitive dyes for enhanced visual detection of amplification. Furthermore, reactions were performed in a portable, non-instrumented nucleic acid amplification (NINA) device that provides a stable heat source for LAMP. The efficacy of several strand displacing DNA polymerases were evaluated in combination with neutral red or phenol red dyes. Colorimetric NINA-LAMP assays targeting Brugia Hha I repeat, Onchocerca volvulus GST1a and Wuchereria bancrofti LDR each exhibit species-specificity and are also highly sensitive, detecting DNA equivalent to 1/10-1/5000th of one microfilaria. Reaction times varied depending on whether a single copy gene (70 minutes, O. volvulus) or repetitive DNA (40 min, B. malayi and W. bancrofti) was employed as a biomarker. The NINA heater can be used to detect multiple infections simultaneously. The accuracy, simplicity and versatility of the technology suggests that colorimetric NINA-LAMP assays are ideally suited for monitoring the success of filariasis control programs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Poole</LastName><ForeName>Catherine B</ForeName><Initials>CB</Initials><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhiru</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alhassan</LastName><ForeName>Andy</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guelig</LastName><ForeName>Dylan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>PATH, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Diesburg</LastName><ForeName>Steven</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>PATH, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tanner</LastName><ForeName>Nathan A</ForeName><Initials>NA</Initials><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yinhua</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Evans</LastName><ForeName>Thomas C</ForeName><Initials>TC</Initials><Suffix>Jr</Suffix><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>LaBarre</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>PATH, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wanji</LastName><ForeName>Samuel</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Research Foundation in Tropical Diseases and Environment, Buea, Cameroon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burton</LastName><ForeName>Robert A</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>PATH, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carlow</LastName><ForeName>Clotilde K S</ForeName><Initials>CK</Initials><AffiliationInfo><Affiliation>New England Biolabs, Ipswich, MA United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>02</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018509">DNA, Helminth</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2000 Jun 15;28(12):E63</RefSource><PMID Version="1">10871386</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Parasitology. 1998 Apr;116 ( Pt 4):327-36</RefSource><PMID 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Carlow</name><name sortKey="Evans, Thomas C" sort="Evans, Thomas C" uniqKey="Evans T" first="Thomas C" last="Evans">Thomas C. Evans</name><name sortKey="Li, Zhiru" sort="Li, Zhiru" uniqKey="Li Z" first="Zhiru" last="Li">Zhiru Li</name><name sortKey="Poole, Catherine B" sort="Poole, Catherine B" uniqKey="Poole C" first="Catherine B" last="Poole">Catherine B. Poole</name><name sortKey="Tanner, Nathan A" sort="Tanner, Nathan A" uniqKey="Tanner N" first="Nathan A" last="Tanner">Nathan A. Tanner</name><name sortKey="Zhang, Yinhua" sort="Zhang, Yinhua" uniqKey="Zhang Y" first="Yinhua" last="Zhang">Yinhua Zhang</name></noCountry><country name="États-Unis"><region name="Washington (État)"><name sortKey="Guelig, Dylan" sort="Guelig, Dylan" uniqKey="Guelig D" first="Dylan" last="Guelig">Dylan Guelig</name></region><name sortKey="Burton, Robert A" sort="Burton, Robert A" uniqKey="Burton R" first="Robert A" last="Burton">Robert A. Burton</name><name sortKey="Diesburg, Steven" sort="Diesburg, Steven" uniqKey="Diesburg S" first="Steven" last="Diesburg">Steven Diesburg</name><name sortKey="Labarre, Paul" sort="Labarre, Paul" uniqKey="Labarre P" first="Paul" last="Labarre">Paul Labarre</name></country><country name="Cameroun"><noRegion><name sortKey="Wanji, Samuel" sort="Wanji, Samuel" uniqKey="Wanji S" first="Samuel" last="Wanji">Samuel Wanji</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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