Platelet and Neutrophil Responses to Gram Positive Pathogens in Patients with Bacteremic Infection
Identifieur interne : 004521 ( Pmc/Curation ); précédent : 004520; suivant : 004522Platelet and Neutrophil Responses to Gram Positive Pathogens in Patients with Bacteremic Infection
Auteurs : Daniel Johansson ; Oonagh Shannon ; Magnus RasmussenSource :
- PLoS ONE [ 1932-6203 ] ; 2011.
Abstract
Many Gram-positive pathogens aggregate and activate platelets
We added isolates of Gram-positive bacteria from 38 patients with a bacteremic infection to blood drawn from the same patient. Aggregometry and flow cytometry were used to assess platelet aggregation and to quantify activation of platelets, neutrophils, and platelet-neutrophils complexes (PNCs) induced by the bacteria. Fifteen healthy persons served as controls. Most isolates of
We have demonstrated for the first time that bacteria isolated from patients with Gram-positive bacteremia can induce platelet activation and aggregation, PNC formation, and neutrophil activation in the same infected host. This underlines the significance of these interactions during infection, which could be a target for future therapies in sepsis.
Url:
DOI: 10.1371/journal.pone.0026928
PubMed: 22140434
PubMed Central: 3226579
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Platelet and Neutrophil Responses to Gram Positive Pathogens in Patients with Bacteremic Infection</title><author><name sortKey="Johansson, Daniel" sort="Johansson, Daniel" uniqKey="Johansson D" first="Daniel" last="Johansson">Daniel Johansson</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Shannon, Oonagh" sort="Shannon, Oonagh" uniqKey="Shannon O" first="Oonagh" last="Shannon">Oonagh Shannon</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rasmussen, Magnus" sort="Rasmussen, Magnus" uniqKey="Rasmussen M" first="Magnus" last="Rasmussen">Magnus Rasmussen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22140434</idno><idno type="pmc">3226579</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226579</idno><idno type="RBID">PMC:3226579</idno><idno type="doi">10.1371/journal.pone.0026928</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">004522</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004522</idno><idno type="wicri:Area/Pmc/Curation">004521</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004521</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Platelet and Neutrophil Responses to Gram Positive Pathogens in Patients with Bacteremic Infection</title><author><name sortKey="Johansson, Daniel" sort="Johansson, Daniel" uniqKey="Johansson D" first="Daniel" last="Johansson">Daniel Johansson</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Shannon, Oonagh" sort="Shannon, Oonagh" uniqKey="Shannon O" first="Oonagh" last="Shannon">Oonagh Shannon</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rasmussen, Magnus" sort="Rasmussen, Magnus" uniqKey="Rasmussen M" first="Magnus" last="Rasmussen">Magnus Rasmussen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Many Gram-positive pathogens aggregate and activate platelets <italic>in vitro</italic> and this has been proposed to contribute to virulence. Platelets can also form complexes with neutrophils but little is however known about platelet and platelet-neutrophil responses in bacterial infection.</p></sec><sec><title>Methodology/Principal Findings</title><p>We added isolates of Gram-positive bacteria from 38 patients with a bacteremic infection to blood drawn from the same patient. Aggregometry and flow cytometry were used to assess platelet aggregation and to quantify activation of platelets, neutrophils, and platelet-neutrophils complexes (PNCs) induced by the bacteria. Fifteen healthy persons served as controls. Most isolates of <italic>Staphylococcus aureus</italic>, beta hemolytic streptococci, and <italic>Enterococcus faecalis</italic> induced aggregation of platelets from their respective hosts, whereas pneumococci failed to do so. <italic>S. aureus</italic> isolates induced platelet aggregation more rapidly in patients than in controls, whereas platelet activation by <italic>S. aureus</italic> was lower in patients than in controls. PNCs were more abundant in baseline samples from patients than in healthy controls and most bacterial isolates induced additional PNC formation and neutrophil activation.</p></sec><sec><title>Conclusion/Significance</title><p>We have demonstrated for the first time that bacteria isolated from patients with Gram-positive bacteremia can induce platelet activation and aggregation, PNC formation, and neutrophil activation in the same infected host. This underlines the significance of these interactions during infection, which could be a target for future therapies in sepsis.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Angus, Dc" uniqKey="Angus D">DC Angus</name></author><author><name sortKey="Linde Zwirble, Wt" uniqKey="Linde Zwirble W">WT Linde-Zwirble</name></author><author><name sortKey="Lidicker, J" uniqKey="Lidicker J">J Lidicker</name></author><author><name sortKey="Clermont, G" uniqKey="Clermont G">G Clermont</name></author><author><name sortKey="Carcillo, J" uniqKey="Carcillo J">J Carcillo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Annane, D" uniqKey="Annane D">D Annane</name></author><author><name sortKey="Bellissant, E" uniqKey="Bellissant E">E Bellissant</name></author><author><name sortKey="Cavaillon, Jm" uniqKey="Cavaillon J">JM Cavaillon</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22140434</article-id><article-id pub-id-type="pmc">3226579</article-id><article-id pub-id-type="publisher-id">PONE-D-11-12942</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0026928</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Bacterial Pathogens</subject><subj-group><subject>Gram Positive</subject><subject>Staphylococci</subject><subject>Streptococci</subject></subj-group></subj-group><subj-group><subject>Immunity</subject><subj-group><subject>Innate Immunity</subject></subj-group></subj-group><subj-group><subject>Host-Pathogen Interaction</subject><subject>Medical Microbiology</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Hematology</subject><subj-group><subject>Platelets</subject></subj-group></subj-group><subj-group><subject>Infectious Diseases</subject><subj-group><subject>Bacterial Diseases</subject><subj-group><subject>Bacteremia</subject><subject>Bloodstream Infections</subject><subject>Group A streptococcal infection</subject><subject>Pneumococcus</subject><subject>Staph Infections</subject><subject>Staphylococcus Aureus</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Platelet and Neutrophil Responses to Gram Positive Pathogens in Patients with Bacteremic Infection</article-title><alt-title alt-title-type="running-head">Platelet and Neutrophil Responses to Bacteremia</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Johansson</surname><given-names>Daniel</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Shannon</surname><given-names>Oonagh</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Rasmussen</surname><given-names>Magnus</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Bereswill</surname><given-names>Stefan</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1">Charité-University Medicine Berlin, Germany</aff><author-notes><corresp id="cor1">* E-mail: <email>Magnus.Rasmussen@med.lu.se</email></corresp><fn fn-type="con"><p>Conceived and designed the experiments: MR OS DJ. Performed the experiments: DJ MR OS. Analyzed the data: DJ MR OS. Contributed reagents/materials/analysis tools: MR OS. Wrote the paper: MR DJ.</p></fn></author-notes><pub-date pub-type="collection"><year>2011</year></pub-date><pub-date pub-type="epub"><day>29</day><month>11</month><year>2011</year></pub-date><volume>6</volume><issue>11</issue><elocation-id>e26928</elocation-id><history><date date-type="received"><day>6</day><month>7</month><year>2011</year></date><date date-type="accepted"><day>6</day><month>10</month><year>2011</year></date></history><permissions><copyright-statement>Johansson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement><copyright-year>2011</copyright-year></permissions><abstract><sec><title>Background</title><p>Many Gram-positive pathogens aggregate and activate platelets <italic>in vitro</italic> and this has been proposed to contribute to virulence. Platelets can also form complexes with neutrophils but little is however known about platelet and platelet-neutrophil responses in bacterial infection.</p></sec><sec><title>Methodology/Principal Findings</title><p>We added isolates of Gram-positive bacteria from 38 patients with a bacteremic infection to blood drawn from the same patient. Aggregometry and flow cytometry were used to assess platelet aggregation and to quantify activation of platelets, neutrophils, and platelet-neutrophils complexes (PNCs) induced by the bacteria. Fifteen healthy persons served as controls. Most isolates of <italic>Staphylococcus aureus</italic>, beta hemolytic streptococci, and <italic>Enterococcus faecalis</italic> induced aggregation of platelets from their respective hosts, whereas pneumococci failed to do so. <italic>S. aureus</italic> isolates induced platelet aggregation more rapidly in patients than in controls, whereas platelet activation by <italic>S. aureus</italic> was lower in patients than in controls. PNCs were more abundant in baseline samples from patients than in healthy controls and most bacterial isolates induced additional PNC formation and neutrophil activation.</p></sec><sec><title>Conclusion/Significance</title><p>We have demonstrated for the first time that bacteria isolated from patients with Gram-positive bacteremia can induce platelet activation and aggregation, PNC formation, and neutrophil activation in the same infected host. This underlines the significance of these interactions during infection, which could be a target for future therapies in sepsis.</p></sec></abstract><counts><page-count count="9"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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