Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1
Identifieur interne : 004505 ( Pmc/Curation ); précédent : 004504; suivant : 004506Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1
Auteurs : Tan N. Truong [États-Unis] ; Hannah Li [États-Unis] ; Young-Kwon Hong [États-Unis] ; Lu Chen [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
Schlemm's canal is an important structure of the conventional aqueous humor outflow pathway and is critically involved in regulating the intraocular pressure. In this study, we report a novel finding that prospero homeobox protein 1 (Prox-1), the master control gene for lymphatic development, is expressed in Schlemm's canal. Moreover, we provide a novel in vivo method of visualizing Schlemm's canal using a transgenic mouse model of Prox-1-green fluorescent protein (GFP). The anatomical location of Prox-1+ Schlemm's canal was further confirmed by in vivo gonioscopic examination and ex vivo immunohistochemical analysis. Additionally, we show that the Schlemm's canal is distinguishable from typical lymphatic vessels by lack of lymphatic vessel endothelial hyaluronan receptor (LYVE-1) expression and absence of apparent sprouting reaction when inflammatory lymphangiogenesis occurred in the cornea. Taken together, our findings offer new insights into Schlemm's canal and provide a new experimental model for live imaging of this critical structure to help further our understanding of the aqueous humor outflow. This may lead to new avenues toward the development of novel therapeutic intervention for relevant diseases, most notably glaucoma.
Url:
DOI: 10.1371/journal.pone.0098245
PubMed: 24827370
PubMed Central: 4020937
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1</title><author><name sortKey="Truong, Tan N" sort="Truong, Tan N" uniqKey="Truong T" first="Tan N." last="Truong">Tan N. Truong</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Graduate Group in Vision Science, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, University of California, Berkeley, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California</wicri:regionArea></affiliation></author><author><name sortKey="Li, Hannah" sort="Li, Hannah" uniqKey="Li H" first="Hannah" last="Li">Hannah Li</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California</wicri:regionArea></affiliation></author><author><name sortKey="Hong, Young Kwon" sort="Hong, Young Kwon" uniqKey="Hong Y" first="Young-Kwon" last="Hong">Young-Kwon Hong</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Surgery and Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery and Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Graduate Group in Vision Science, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, University of California, Berkeley, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>The Francis I. 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Truong</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Graduate Group in Vision Science, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, University of California, Berkeley, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California</wicri:regionArea></affiliation></author><author><name sortKey="Li, Hannah" sort="Li, Hannah" uniqKey="Li H" first="Hannah" last="Li">Hannah Li</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California</wicri:regionArea></affiliation></author><author><name sortKey="Hong, Young Kwon" sort="Hong, Young Kwon" uniqKey="Hong Y" first="Young-Kwon" last="Hong">Young-Kwon Hong</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Surgery and Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery and Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Graduate Group in Vision Science, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, University of California, Berkeley, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>The Francis I. Proctor Foundation for Research in Ophthalmology, University of California San Francisco, San Francisco, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Francis I. Proctor Foundation for Research in Ophthalmology, University of California San Francisco, San Francisco, California</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Schlemm's canal is an important structure of the conventional aqueous humor outflow pathway and is critically involved in regulating the intraocular pressure. In this study, we report a novel finding that prospero homeobox protein 1 (Prox-1), the master control gene for lymphatic development, is expressed in Schlemm's canal. Moreover, we provide a novel in vivo method of visualizing Schlemm's canal using a transgenic mouse model of Prox-1-green fluorescent protein (GFP). The anatomical location of Prox-1<sup>+</sup> Schlemm's canal was further confirmed by in vivo gonioscopic examination and ex vivo immunohistochemical analysis. Additionally, we show that the Schlemm's canal is distinguishable from typical lymphatic vessels by lack of lymphatic vessel endothelial hyaluronan receptor (LYVE-1) expression and absence of apparent sprouting reaction when inflammatory lymphangiogenesis occurred in the cornea. Taken together, our findings offer new insights into Schlemm's canal and provide a new experimental model for live imaging of this critical structure to help further our understanding of the aqueous humor outflow. This may lead to new avenues toward the development of novel therapeutic intervention for relevant diseases, most notably glaucoma.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Maepea, O" uniqKey="Maepea O">O Maepea</name></author><author><name sortKey="Bill, A" uniqKey="Bill A">A Bill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cook, C" uniqKey="Cook C">C Cook</name></author><author><name sortKey="Foster, P" uniqKey="Foster P">P Foster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramos, Rf" uniqKey="Ramos R">RF Ramos</name></author><author><name sortKey="Hoying, Jb" uniqKey="Hoying J">JB Hoying</name></author><author><name sortKey="Witte, Mh" uniqKey="Witte M">MH Witte</name></author><author><name sortKey="Daniel Stamer, W" uniqKey="Daniel Stamer W">W Daniel Stamer</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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Stamer</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24827370</article-id><article-id pub-id-type="pmc">4020937</article-id><article-id pub-id-type="publisher-id">PONE-D-13-55114</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0098245</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Ocular System</subject><subj-group><subject>Ocular Anatomy</subject></subj-group></subj-group></subj-group><subj-group><subject>Immunology</subject><subj-group><subject>Immune Response</subject><subj-group><subject>Inflammation</subject></subj-group></subj-group><subj-group><subject>Immunity</subject></subj-group></subj-group><subj-group><subject>Neuroscience</subject><subj-group><subject>Sensory Systems</subject><subj-group><subject>Visual System</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine and Health Sciences</subject><subj-group><subject>Ophthalmology</subject><subj-group><subject>Eye Diseases</subject><subj-group><subject>Glaucoma</subject></subj-group></subj-group><subj-group><subject>Corneal Disorders</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Research and Analysis Methods</subject><subj-group><subject>Histochemistry and Cytochemistry Techniques</subject><subj-group><subject>Immunohistochemistry Techniques</subject><subj-group><subject>Immunohistochemical Analysis</subject></subj-group></subj-group></subj-group><subj-group><subject>Immunologic Techniques</subject></subj-group><subj-group><subject>Model Organisms</subject><subj-group><subject>Animal Models</subject><subj-group><subject>Mouse Models</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1</article-title><alt-title alt-title-type="running-head">Schlemm's Canal Live Imaging in Prox-1-GFP Mouse</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Truong</surname><given-names>Tan N.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Hannah</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hong</surname><given-names>Young-Kwon</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Lu</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Graduate Group in Vision Science, University of California, Berkeley, California, United States of America</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, California, United States of America</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Department of Surgery and Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America</addr-line></aff><aff id="aff4"><label>4</label><addr-line>The Francis I. Proctor Foundation for Research in Ophthalmology, University of California San Francisco, San Francisco, California, United States of America</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Acott</surname><given-names>Ted S.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Casey Eye Institute, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>chenlu@berkeley.edu.</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: TT LC. Performed the experiments: TT HL. Analyzed the data: TT LC. Contributed reagents/materials/analysis tools: YKH. Wrote the paper: TT LC.</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>14</day><month>5</month><year>2014</year></pub-date><volume>9</volume><issue>5</issue><elocation-id>e98245</elocation-id><history><date date-type="received"><day>30</day><month>12</month><year>2013</year></date><date date-type="accepted"><day>29</day><month>4</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Truong et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>Schlemm's canal is an important structure of the conventional aqueous humor outflow pathway and is critically involved in regulating the intraocular pressure. In this study, we report a novel finding that prospero homeobox protein 1 (Prox-1), the master control gene for lymphatic development, is expressed in Schlemm's canal. Moreover, we provide a novel in vivo method of visualizing Schlemm's canal using a transgenic mouse model of Prox-1-green fluorescent protein (GFP). The anatomical location of Prox-1<sup>+</sup> Schlemm's canal was further confirmed by in vivo gonioscopic examination and ex vivo immunohistochemical analysis. Additionally, we show that the Schlemm's canal is distinguishable from typical lymphatic vessels by lack of lymphatic vessel endothelial hyaluronan receptor (LYVE-1) expression and absence of apparent sprouting reaction when inflammatory lymphangiogenesis occurred in the cornea. Taken together, our findings offer new insights into Schlemm's canal and provide a new experimental model for live imaging of this critical structure to help further our understanding of the aqueous humor outflow. This may lead to new avenues toward the development of novel therapeutic intervention for relevant diseases, most notably glaucoma.</p></abstract><funding-group><funding-statement>This work is supported in part by research grants from National Institutes of Health and the University of California at Berkeley. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="8"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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