Recombinant Human Endostatin Endostar Suppresses Angiogenesis and Lymphangiogenesis of Malignant Pleural Effusion in Mice
Identifieur interne : 004462 ( Pmc/Curation ); précédent : 004461; suivant : 004463Recombinant Human Endostatin Endostar Suppresses Angiogenesis and Lymphangiogenesis of Malignant Pleural Effusion in Mice
Auteurs : Xingqun Ma [République populaire de Chine] ; Yanwen Yao [République populaire de Chine] ; Dongmei Yuan [République populaire de Chine] ; Hongbing Liu [République populaire de Chine] ; Shouju Wang [République populaire de Chine] ; Changsheng Zhou [République populaire de Chine] ; Yong Song [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
Malignant pleural effusion (MPE) is a common complication of lung cancer. One widely used treatment for MPE is Endostar, a recombined humanized endostatin based treatment. However, the mechanism of this treatment is still unclear. The aim of this study was to investigate the effects of Endostar in mice with MPE.
Lewis lung carcinoma (LLC) cell line expressing enhanced green fluorescent protein (EGFP) was injected into pleural cavity to establish MPE mice model. Mice were randomly divided into four groups. High dose of Endostar (30 mg/kg), low dose of Endostar (8 mg/kg), normal saline, or Bevacizumab (5 mg/kg) was respectively injected into pleural cavity three times with 3-day interval in each group. Transverse computed tomography (CT) was performed to observe pleural fluid formation 14 days after LLC cells injection. Mice were anesthetized and sacrificed 3 days after final administration. The volume of pleural effusion n was measured using 1 ml syringe. Micro blood vessel density (MVD), Lymphatic micro vessel density (LMVD), the expression level of vascular endothelial growth factor A (VEGF-A) and VEGF-C were observed by immunohistochemistry (IHC) staining.
The volume of pleural effusion as well as the number of pleural tumor foci, MVD and the expression of VEGF-A were significantly reduced in high dose of Endostar treat group. More importantly, LMVD and the expression of VEGF-C were markedly lower in treat group than those in the other three control groups.
Our work demonstrated that Endostar played an efficient anti-cancer role in MPE through its suppressive effect on angiogenesis and lymphangiogenesis, which provided a certain theoretical basis for the effectiveness of Endostar on the MPE treatment.
Url:
DOI: 10.1371/journal.pone.0053449
PubMed: 23285296
PubMed Central: 3532165
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :004463
Links to Exploration step
PMC:3532165Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinant Human Endostatin Endostar Suppresses Angiogenesis and Lymphangiogenesis of Malignant Pleural Effusion in Mice</title><author><name sortKey="Ma, Xingqun" sort="Ma, Xingqun" uniqKey="Ma X" first="Xingqun" last="Ma">Xingqun Ma</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Yao, Yanwen" sort="Yao, Yanwen" uniqKey="Yao Y" first="Yanwen" last="Yao">Yanwen Yao</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, Dongmei" sort="Yuan, Dongmei" uniqKey="Yuan D" first="Dongmei" last="Yuan">Dongmei Yuan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Hongbing" sort="Liu, Hongbing" uniqKey="Liu H" first="Hongbing" last="Liu">Hongbing Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Shouju" sort="Wang, Shouju" uniqKey="Wang S" first="Shouju" last="Wang">Shouju Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Changsheng" sort="Zhou, Changsheng" uniqKey="Zhou C" first="Changsheng" last="Zhou">Changsheng Zhou</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Song, Yong" sort="Song, Yong" uniqKey="Song Y" first="Yong" last="Song">Yong Song</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23285296</idno><idno type="pmc">3532165</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3532165</idno><idno type="RBID">PMC:3532165</idno><idno type="doi">10.1371/journal.pone.0053449</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">004463</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004463</idno><idno type="wicri:Area/Pmc/Curation">004462</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004462</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Recombinant Human Endostatin Endostar Suppresses Angiogenesis and Lymphangiogenesis of Malignant Pleural Effusion in Mice</title><author><name sortKey="Ma, Xingqun" sort="Ma, Xingqun" uniqKey="Ma X" first="Xingqun" last="Ma">Xingqun Ma</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Yao, Yanwen" sort="Yao, Yanwen" uniqKey="Yao Y" first="Yanwen" last="Yao">Yanwen Yao</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, Dongmei" sort="Yuan, Dongmei" uniqKey="Yuan D" first="Dongmei" last="Yuan">Dongmei Yuan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Hongbing" sort="Liu, Hongbing" uniqKey="Liu H" first="Hongbing" last="Liu">Hongbing Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Shouju" sort="Wang, Shouju" uniqKey="Wang S" first="Shouju" last="Wang">Shouju Wang</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Changsheng" sort="Zhou, Changsheng" uniqKey="Zhou C" first="Changsheng" last="Zhou">Changsheng Zhou</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Song, Yong" sort="Song, Yong" uniqKey="Song Y" first="Yong" last="Song">Yong Song</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Malignant pleural effusion (MPE) is a common complication of lung cancer. One widely used treatment for MPE is Endostar, a recombined humanized endostatin based treatment. However, the mechanism of this treatment is still unclear. The aim of this study was to investigate the effects of Endostar in mice with MPE.</p></sec><sec><title>Methods and Materials</title><p>Lewis lung carcinoma (LLC) cell line expressing enhanced green fluorescent protein (EGFP) was injected into pleural cavity to establish MPE mice model. Mice were randomly divided into four groups. High dose of Endostar (30 mg/kg), low dose of Endostar (8 mg/kg), normal saline, or Bevacizumab (5 mg/kg) was respectively injected into pleural cavity three times with 3-day interval in each group. Transverse computed tomography (CT) was performed to observe pleural fluid formation 14 days after LLC cells injection. Mice were anesthetized and sacrificed 3 days after final administration. The volume of pleural effusion n was measured using 1 ml syringe. Micro blood vessel density (MVD), Lymphatic micro vessel density (LMVD), the expression level of vascular endothelial growth factor A (VEGF-A) and VEGF-C were observed by immunohistochemistry (IHC) staining.</p></sec><sec><title>Results</title><p>The volume of pleural effusion as well as the number of pleural tumor foci, MVD and the expression of VEGF-A were significantly reduced in high dose of Endostar treat group. More importantly, LMVD and the expression of VEGF-C were markedly lower in treat group than those in the other three control groups.</p></sec><sec><title>Conclusion</title><p>Our work demonstrated that Endostar played an efficient anti-cancer role in MPE through its suppressive effect on angiogenesis and lymphangiogenesis, which provided a certain theoretical basis for the effectiveness of Endostar on the MPE treatment.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Siegel, R" uniqKey="Siegel R">R Siegel</name></author><author><name sortKey="Naishadham, D" uniqKey="Naishadham D">D Naishadham</name></author><author><name sortKey="Jemal, A" uniqKey="Jemal A">A Jemal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Yc" uniqKey="Lee Y">YC Lee</name></author><author><name sortKey="Light, Rw" uniqKey="Light R">RW Light</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaifi, Jt" uniqKey="Kaifi J">JT Kaifi</name></author><author><name sortKey="Toth, Jw" uniqKey="Toth J">JW Toth</name></author><author><name sortKey="Gusani, Nj" uniqKey="Gusani N">NJ Gusani</name></author><author><name sortKey="Kimchi, Et" uniqKey="Kimchi E">ET Kimchi</name></author><author><name sortKey="Staveley O Carroll, Kf" uniqKey="Staveley O Carroll K">KF Staveley-O'Carroll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jantz, Ma" uniqKey="Jantz M">MA Jantz</name></author><author><name sortKey="Antony, Vb" uniqKey="Antony V">VB Antony</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mundhenke, C" uniqKey="Mundhenke C">C Mundhenke</name></author><author><name sortKey="Thomas, Jp" uniqKey="Thomas J">JP Thomas</name></author><author><name sortKey="Wilding, G" uniqKey="Wilding G">G Wilding</name></author><author><name sortKey="Lee, Ft" uniqKey="Lee F">FT Lee</name></author><author><name sortKey="Kelzc, F" uniqKey="Kelzc F">F Kelzc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chernow, B" uniqKey="Chernow B">B Chernow</name></author><author><name sortKey="Sahn, Sa" uniqKey="Sahn S">SA Sahn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lynch, Tj" uniqKey="Lynch T">TJ Lynch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagahashi, M" uniqKey="Nagahashi M">M Nagahashi</name></author><author><name sortKey="Ramachandran, S" uniqKey="Ramachandran S">S Ramachandran</name></author><author><name sortKey="Rashid, Om" uniqKey="Rashid O">OM Rashid</name></author><author><name sortKey="Takabe, K" uniqKey="Takabe K">K Takabe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="O Reilly, Ms" uniqKey="O Reilly M">MS O'Reilly</name></author><author><name sortKey="Boehm, T" uniqKey="Boehm T">T Boehm</name></author><author><name sortKey="Shing, Y" uniqKey="Shing Y">Y Shing</name></author><author><name sortKey="Fukai, N" uniqKey="Fukai N">N Fukai</name></author><author><name sortKey="Vasios, G" uniqKey="Vasios G">G Vasios</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brideau, G" uniqKey="Brideau G">G Brideau</name></author><author><name sortKey="Makinen, Mj" uniqKey="Makinen M">MJ Makinen</name></author><author><name sortKey="Elamaa, H" uniqKey="Elamaa H">H Elamaa</name></author><author><name sortKey="Tu, H" uniqKey="Tu H">H Tu</name></author><author><name sortKey="Nilsson, G" uniqKey="Nilsson G">G Nilsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fukumoto, S" uniqKey="Fukumoto S">S Fukumoto</name></author><author><name sortKey="Morifuji, M" uniqKey="Morifuji M">M Morifuji</name></author><author><name sortKey="Katakura, Y" uniqKey="Katakura Y">Y Katakura</name></author><author><name sortKey="Ohishi, M" uniqKey="Ohishi M">M Ohishi</name></author><author><name sortKey="Nakamura, S" uniqKey="Nakamura S">S Nakamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nasreen, N" uniqKey="Nasreen N">N Nasreen</name></author><author><name sortKey="Mohammed, Ka" uniqKey="Mohammed K">KA Mohammed</name></author><author><name sortKey="Brown, S" uniqKey="Brown S">S Brown</name></author><author><name sortKey="Su, Y" uniqKey="Su Y">Y Su</name></author><author><name sortKey="Sriram, Ps" uniqKey="Sriram P">PS Sriram</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eder, Jp" uniqKey="Eder J">JP Eder</name></author><author><name sortKey="Supko, Jg" uniqKey="Supko J">JG Supko</name></author><author><name sortKey="Clark, Jw" uniqKey="Clark J">JW Clark</name></author><author><name sortKey="Puchalski, Ta" uniqKey="Puchalski T">TA Puchalski</name></author><author><name sortKey="Garcia Carbonero, R" uniqKey="Garcia Carbonero R">R Garcia-Carbonero</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ling, Y" uniqKey="Ling Y">Y Ling</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author><author><name sortKey="Lu, N" uniqKey="Lu N">N Lu</name></author><author><name sortKey="You, Qd" uniqKey="You Q">QD You</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, X" uniqKey="Ma X">X Ma</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y Sun</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z Yang</name></author><author><name sortKey="Song, Y" uniqKey="Song Y">Y Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dong, X" uniqKey="Dong X">X Dong</name></author><author><name sortKey="Zhao, X" uniqKey="Zhao X">X Zhao</name></author><author><name sortKey="Xiao, T" uniqKey="Xiao T">T Xiao</name></author><author><name sortKey="Tian, H" uniqKey="Tian H">H Tian</name></author><author><name sortKey="Yun, C" uniqKey="Yun C">C Yun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fang, F" uniqKey="Fang F">F Fang</name></author><author><name sortKey="Chen, P" uniqKey="Chen P">P Chen</name></author><author><name sortKey="Wu, X" uniqKey="Wu X">X Wu</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobold, S" uniqKey="Kobold S">S Kobold</name></author><author><name sortKey="Hegewisch Becker, S" uniqKey="Hegewisch Becker S">S Hegewisch-Becker</name></author><author><name sortKey="Oechsle, K" uniqKey="Oechsle K">K Oechsle</name></author><author><name sortKey="Jordan, K" uniqKey="Jordan K">K Jordan</name></author><author><name sortKey="Bokemeyer, C" uniqKey="Bokemeyer C">C Bokemeyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wei, Hm" uniqKey="Wei H">HM Wei</name></author><author><name sortKey="Qin, Sk" uniqKey="Qin S">SK Qin</name></author><author><name sortKey="Yin, Xj" uniqKey="Yin X">XJ Yin</name></author><author><name sortKey="Chen, Yl" uniqKey="Chen Y">YL Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wei, S" uniqKey="Wei S">S Wei</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y Sun</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z Yang</name></author><author><name sortKey="Song, Y" uniqKey="Song Y">Y Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stathopoulos, Gt" uniqKey="Stathopoulos G">GT Stathopoulos</name></author><author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z Zhu</name></author><author><name sortKey="Everhart, Mb" uniqKey="Everhart M">MB Everhart</name></author><author><name sortKey="Kalomenidis, I" uniqKey="Kalomenidis I">I Kalomenidis</name></author><author><name sortKey="Lawson, We" uniqKey="Lawson W">WE Lawson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mohammed, Ra" uniqKey="Mohammed R">RA Mohammed</name></author><author><name sortKey="Green, A" uniqKey="Green A">A Green</name></author><author><name sortKey="El Shikh, S" uniqKey="El Shikh S">S El-Shikh</name></author><author><name sortKey="Paish, Ec" uniqKey="Paish E">EC Paish</name></author><author><name sortKey="Ellis, Io" uniqKey="Ellis I">IO Ellis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rolny, C" uniqKey="Rolny C">C Rolny</name></author><author><name sortKey="Mazzone, M" uniqKey="Mazzone M">M Mazzone</name></author><author><name sortKey="Tugues, S" uniqKey="Tugues S">S Tugues</name></author><author><name sortKey="Laoui, D" uniqKey="Laoui D">D Laoui</name></author><author><name sortKey="Johansson, I" uniqKey="Johansson I">I Johansson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Juttner, S" uniqKey="Juttner S">S Juttner</name></author><author><name sortKey="Wissmann, C" uniqKey="Wissmann C">C Wissmann</name></author><author><name sortKey="Jons, T" uniqKey="Jons T">T Jons</name></author><author><name sortKey="Vieth, M" uniqKey="Vieth M">M Vieth</name></author><author><name sortKey="Hertel, J" uniqKey="Hertel J">J Hertel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stathopoulos, Gt" uniqKey="Stathopoulos G">GT Stathopoulos</name></author><author><name sortKey="Kollintza, A" uniqKey="Kollintza A">A Kollintza</name></author><author><name sortKey="Moschos, C" uniqKey="Moschos C">C Moschos</name></author><author><name sortKey="Psallidas, I" uniqKey="Psallidas I">I Psallidas</name></author><author><name sortKey="Sherrill, Tp" uniqKey="Sherrill T">TP Sherrill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bose, D" uniqKey="Bose D">D Bose</name></author><author><name sortKey="Meric Bernstam, F" uniqKey="Meric Bernstam F">F Meric-Bernstam</name></author><author><name sortKey="Hofstetter, W" uniqKey="Hofstetter W">W Hofstetter</name></author><author><name sortKey="Reardon, Da" uniqKey="Reardon D">DA Reardon</name></author><author><name sortKey="Flaherty, Kt" uniqKey="Flaherty K">KT Flaherty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gerber, Hp" uniqKey="Gerber H">HP Gerber</name></author><author><name sortKey="Ferrara, N" uniqKey="Ferrara N">N Ferrara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Presta, Lg" uniqKey="Presta L">LG Presta</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="O Connor, Sj" uniqKey="O Connor S">SJ O'Connor</name></author><author><name sortKey="Chisholm, V" uniqKey="Chisholm V">V Chisholm</name></author><author><name sortKey="Meng, Yg" uniqKey="Meng Y">YG Meng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hajitou, A" uniqKey="Hajitou A">A Hajitou</name></author><author><name sortKey="Grignet, C" uniqKey="Grignet C">C Grignet</name></author><author><name sortKey="Devy, L" uniqKey="Devy L">L Devy</name></author><author><name sortKey="Berndt, S" uniqKey="Berndt S">S Berndt</name></author><author><name sortKey="Blacher, S" uniqKey="Blacher S">S Blacher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lohela, M" uniqKey="Lohela M">M Lohela</name></author><author><name sortKey="Bry, M" uniqKey="Bry M">M Bry</name></author><author><name sortKey="Tammela, T" uniqKey="Tammela T">T Tammela</name></author><author><name sortKey="Alitalo, K" uniqKey="Alitalo K">K Alitalo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Avraamides, Cj" uniqKey="Avraamides C">CJ Avraamides</name></author><author><name sortKey="Garmy Susini, B" uniqKey="Garmy Susini B">B Garmy-Susini</name></author><author><name sortKey="Varner, Ja" uniqKey="Varner J">JA Varner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shibuya, M" uniqKey="Shibuya M">M Shibuya</name></author><author><name sortKey="Claesson Welsh, L" uniqKey="Claesson Welsh L">L Claesson-Welsh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reck, M" uniqKey="Reck M">M Reck</name></author><author><name sortKey="Crino, L" uniqKey="Crino L">L Crino</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23285296</article-id><article-id pub-id-type="pmc">3532165</article-id><article-id pub-id-type="publisher-id">PONE-D-12-24534</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0053449</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Anatomy and Physiology</subject><subj-group><subject>Endocrine System</subject><subj-group><subject>Endocrine Physiology</subject><subj-group><subject>Epidermal Growth Factor</subject></subj-group></subj-group></subj-group></subj-group><subj-group><subject>Model Organisms</subject><subj-group><subject>Animal Models</subject><subj-group><subject>Mouse</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Anatomy and Physiology</subject><subj-group><subject>Endocrine System</subject><subj-group><subject>Endocrine Physiology</subject><subj-group><subject>Epidermal Growth Factor</subject></subj-group></subj-group></subj-group></subj-group><subj-group><subject>Clinical Research Design</subject><subj-group><subject>Animal Models of Disease</subject></subj-group></subj-group><subj-group><subject>Endocrinology</subject><subj-group><subject>Endocrine Physiology</subject><subj-group><subject>Epidermal Growth Factor</subject></subj-group></subj-group></subj-group><subj-group><subject>Oncology</subject><subj-group><subject>Cancer Treatment</subject><subj-group><subject>Antiangiogenesis Therapy</subject></subj-group></subj-group><subj-group><subject>Cancers and Neoplasms</subject><subj-group><subject>Lung and Intrathoracic Tumors</subject></subj-group></subj-group><subj-group><subject>Basic Cancer Research</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Recombinant Human Endostatin Endostar Suppresses Angiogenesis and Lymphangiogenesis of Malignant Pleural Effusion in Mice</article-title><alt-title alt-title-type="running-head">Endostar Suppresses Lymphangiogenesis of MPE</alt-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Ma</surname><given-names>Xingqun</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Yao</surname><given-names>Yanwen</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yuan</surname><given-names>Dongmei</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Hongbing</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Shouju</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Changsheng</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Song</surname><given-names>Yong</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Kalinichenko</surname><given-names>Vladimir V.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Cincinnati Children's Hospital Medical Center, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>yong_song6310@yahoo.com</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: XQM YS. Performed the experiments: XQM SJW CSZ. Analyzed the data: XQM YWY. Contributed reagents/materials/analysis tools: XQM DMY HBL. Wrote the paper: XQM YWY YS.</p></fn></author-notes><pub-date pub-type="collection"><year>2012</year></pub-date><pub-date pub-type="epub"><day>28</day><month>12</month><year>2012</year></pub-date><volume>7</volume><issue>12</issue><elocation-id>e53449</elocation-id><history><date date-type="received"><day>9</day><month>8</month><year>2012</year></date><date date-type="accepted"><day>28</day><month>11</month><year>2012</year></date></history><permissions><copyright-year>2012</copyright-year><copyright-holder>Ma et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><sec><title>Background</title><p>Malignant pleural effusion (MPE) is a common complication of lung cancer. One widely used treatment for MPE is Endostar, a recombined humanized endostatin based treatment. However, the mechanism of this treatment is still unclear. The aim of this study was to investigate the effects of Endostar in mice with MPE.</p></sec><sec><title>Methods and Materials</title><p>Lewis lung carcinoma (LLC) cell line expressing enhanced green fluorescent protein (EGFP) was injected into pleural cavity to establish MPE mice model. Mice were randomly divided into four groups. High dose of Endostar (30 mg/kg), low dose of Endostar (8 mg/kg), normal saline, or Bevacizumab (5 mg/kg) was respectively injected into pleural cavity three times with 3-day interval in each group. Transverse computed tomography (CT) was performed to observe pleural fluid formation 14 days after LLC cells injection. Mice were anesthetized and sacrificed 3 days after final administration. The volume of pleural effusion n was measured using 1 ml syringe. Micro blood vessel density (MVD), Lymphatic micro vessel density (LMVD), the expression level of vascular endothelial growth factor A (VEGF-A) and VEGF-C were observed by immunohistochemistry (IHC) staining.</p></sec><sec><title>Results</title><p>The volume of pleural effusion as well as the number of pleural tumor foci, MVD and the expression of VEGF-A were significantly reduced in high dose of Endostar treat group. More importantly, LMVD and the expression of VEGF-C were markedly lower in treat group than those in the other three control groups.</p></sec><sec><title>Conclusion</title><p>Our work demonstrated that Endostar played an efficient anti-cancer role in MPE through its suppressive effect on angiogenesis and lymphangiogenesis, which provided a certain theoretical basis for the effectiveness of Endostar on the MPE treatment.</p></sec></abstract><funding-group><funding-statement>This research was supported by the Natural Science Fund of Jiangsu Province (BK2011658) to Song Yong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="10"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004462 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 004462 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:3532165
|texte= Recombinant Human Endostatin Endostar Suppresses Angiogenesis and Lymphangiogenesis of Malignant Pleural Effusion in Mice
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:23285296" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |