ZSTK474, a specific class I phosphatidylinositol 3-kinase inhibitor, induces G1 arrest and autophagy in human breast cancer MCF-7 cells
Identifieur interne : 004258 ( Pmc/Curation ); précédent : 004257; suivant : 004259ZSTK474, a specific class I phosphatidylinositol 3-kinase inhibitor, induces G1 arrest and autophagy in human breast cancer MCF-7 cells
Auteurs : Yaochen Wang [République populaire de Chine] ; Jing Liu [République populaire de Chine] ; Yuling Qiu [République populaire de Chine] ; Meihua Jin [République populaire de Chine] ; Xi Chen [République populaire de Chine] ; Guanwei Fan [République populaire de Chine] ; Ran Wang [République populaire de Chine] ; Dexin Kong [République populaire de Chine]Source :
- Oncotarget [ 1949-2553 ] ; 2016.
Abstract
Multifaceted activities of class I phosphatidylinositol 3-kinase (PI3K) inhibitor ZSTK474 were investigated on human breast cancer cell MCF-7. ZSTK474 inhibited proliferation of MCF-7 cells potently. Flow cytometric analysis indicated that ZSTK474 induced cell cycle arrest at G1 phase, but no obvious apoptosis occurred. Western blot analysis suggested that blockade of PI3K/Akt/GSK-3β/cyclin D1/p-Rb pathway might contribute to the G1 arrest induced. Moreover, we demonstrated that ZSTK474 induced autophagy in MCF-7 cells by use of various assays including monodansylcadaverine (MDC) staining, transmission electron microscopy (TEM), tandem mRFP-GFP-LC3 fluorescence microscopy, and western blot detection of the autophagy protein markers of LC3B II, p62 and Atg 5. Inhibition of class I PI3K and the downstream mTOR might be involved in the autophagy-inducing effect. Combinational use of ZSTK474 and autophagy inhibitors enhanced cell viability, suggesting ZSTK474-induced autophagy might contribute to the antitumor activity. Our report supports the application of ZSTK474, which is being evaluated in clinical trials, for breast cancer therapy.
Url:
DOI: 10.18632/oncotarget.7658
PubMed: 26918351
PubMed Central: 4991426
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :004259
Links to Exploration step
PMC:4991426Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">ZSTK474, a specific class I phosphatidylinositol 3-kinase inhibitor, induces G1 arrest and autophagy in human breast cancer MCF-7 cells</title>
<author><name sortKey="Wang, Yaochen" sort="Wang, Yaochen" uniqKey="Wang Y" first="Yaochen" last="Wang">Yaochen Wang</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A2"> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Jing" sort="Liu, Jing" uniqKey="Liu J" first="Jing" last="Liu">Jing Liu</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A2"> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Qiu, Yuling" sort="Qiu, Yuling" uniqKey="Qiu Y" first="Yuling" last="Qiu">Yuling Qiu</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jin, Meihua" sort="Jin, Meihua" uniqKey="Jin M" first="Meihua" last="Jin">Meihua Jin</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chen, Xi" sort="Chen, Xi" uniqKey="Chen X" first="Xi" last="Chen">Xi Chen</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fan, Guanwei" sort="Fan, Guanwei" uniqKey="Fan G" first="Guanwei" last="Fan">Guanwei Fan</name>
<affiliation wicri:level="1"><nlm:aff id="A3"> State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Nankai Dsitrict, Tianjin 300193, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Nankai Dsitrict, Tianjin 300193</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Ran" sort="Wang, Ran" uniqKey="Wang R" first="Ran" last="Wang">Ran Wang</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kong, Dexin" sort="Kong, Dexin" uniqKey="Kong D" first="Dexin" last="Kong">Dexin Kong</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A2"> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26918351</idno>
<idno type="pmc">4991426</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991426</idno>
<idno type="RBID">PMC:4991426</idno>
<idno type="doi">10.18632/oncotarget.7658</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">004259</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004259</idno>
<idno type="wicri:Area/Pmc/Curation">004258</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004258</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">ZSTK474, a specific class I phosphatidylinositol 3-kinase inhibitor, induces G1 arrest and autophagy in human breast cancer MCF-7 cells</title>
<author><name sortKey="Wang, Yaochen" sort="Wang, Yaochen" uniqKey="Wang Y" first="Yaochen" last="Wang">Yaochen Wang</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A2"> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Jing" sort="Liu, Jing" uniqKey="Liu J" first="Jing" last="Liu">Jing Liu</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A2"> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Qiu, Yuling" sort="Qiu, Yuling" uniqKey="Qiu Y" first="Yuling" last="Qiu">Yuling Qiu</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jin, Meihua" sort="Jin, Meihua" uniqKey="Jin M" first="Meihua" last="Jin">Meihua Jin</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chen, Xi" sort="Chen, Xi" uniqKey="Chen X" first="Xi" last="Chen">Xi Chen</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fan, Guanwei" sort="Fan, Guanwei" uniqKey="Fan G" first="Guanwei" last="Fan">Guanwei Fan</name>
<affiliation wicri:level="1"><nlm:aff id="A3"> State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Nankai Dsitrict, Tianjin 300193, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Nankai Dsitrict, Tianjin 300193</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Ran" sort="Wang, Ran" uniqKey="Wang R" first="Ran" last="Wang">Ran Wang</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kong, Dexin" sort="Kong, Dexin" uniqKey="Kong D" first="Dexin" last="Kong">Dexin Kong</name>
<affiliation wicri:level="1"><nlm:aff id="A1"> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A2"> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea> Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Oncotarget</title>
<idno type="eISSN">1949-2553</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Multifaceted activities of class I phosphatidylinositol 3-kinase (PI3K) inhibitor ZSTK474 were investigated on human breast cancer cell MCF-7. ZSTK474 inhibited proliferation of MCF-7 cells potently. Flow cytometric analysis indicated that ZSTK474 induced cell cycle arrest at G1 phase, but no obvious apoptosis occurred. Western blot analysis suggested that blockade of PI3K/Akt/GSK-3β/cyclin D1/p-Rb pathway might contribute to the G1 arrest induced. Moreover, we demonstrated that ZSTK474 induced autophagy in MCF-7 cells by use of various assays including monodansylcadaverine (MDC) staining, transmission electron microscopy (TEM), tandem mRFP-GFP-LC3 fluorescence microscopy, and western blot detection of the autophagy protein markers of LC3B II, p62 and Atg 5. Inhibition of class I PI3K and the downstream mTOR might be involved in the autophagy-inducing effect. Combinational use of ZSTK474 and autophagy inhibitors enhanced cell viability, suggesting ZSTK474-induced autophagy might contribute to the antitumor activity. Our report supports the application of ZSTK474, which is being evaluated in clinical trials, for breast cancer therapy.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Torre, La" uniqKey="Torre L">LA Torre</name>
</author>
<author><name sortKey="Bray, F" uniqKey="Bray F">F Bray</name>
</author>
<author><name sortKey="Siegel, Rl" uniqKey="Siegel R">RL Siegel</name>
</author>
<author><name sortKey="Ferlay, J" uniqKey="Ferlay J">J Ferlay</name>
</author>
<author><name sortKey="Lortet Tieulent, J" uniqKey="Lortet Tieulent J">J Lortet-Tieulent</name>
</author>
<author><name sortKey="Jemal, A" uniqKey="Jemal A">A Jemal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Desantis, Ce" uniqKey="Desantis C">CE DeSantis</name>
</author>
<author><name sortKey="Lin, Cc" uniqKey="Lin C">CC Lin</name>
</author>
<author><name sortKey="Mariotto, Ab" uniqKey="Mariotto A">AB Mariotto</name>
</author>
<author><name sortKey="Siegel, Rl" uniqKey="Siegel R">RL Siegel</name>
</author>
<author><name sortKey="Stein, Kd" uniqKey="Stein K">KD Stein</name>
</author>
<author><name sortKey="Kramer, Jl" uniqKey="Kramer J">JL Kramer</name>
</author>
<author><name sortKey="Alteri, R" uniqKey="Alteri R">R Alteri</name>
</author>
<author><name sortKey="Robbins, As" uniqKey="Robbins A">AS Robbins</name>
</author>
<author><name sortKey="Jemal, A" uniqKey="Jemal A">A Jemal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Samuels, Y" uniqKey="Samuels Y">Y Samuels</name>
</author>
<author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
<author><name sortKey="Bardelli, A" uniqKey="Bardelli A">A Bardelli</name>
</author>
<author><name sortKey="Silliman, N" uniqKey="Silliman N">N Silliman</name>
</author>
<author><name sortKey="Ptak, J" uniqKey="Ptak J">J Ptak</name>
</author>
<author><name sortKey="Szabo, S" uniqKey="Szabo S">S Szabo</name>
</author>
<author><name sortKey="Yan, H" uniqKey="Yan H">H Yan</name>
</author>
<author><name sortKey="Gazdar, A" uniqKey="Gazdar A">A Gazdar</name>
</author>
<author><name sortKey="Powell, Sm" uniqKey="Powell S">SM Powell</name>
</author>
<author><name sortKey="Riggins, Gj" uniqKey="Riggins G">GJ Riggins</name>
</author>
<author><name sortKey="Willson, Jk" uniqKey="Willson J">JK Willson</name>
</author>
<author><name sortKey="Markowitz, S" uniqKey="Markowitz S">S Markowitz</name>
</author>
<author><name sortKey="Kinzler, Kw" uniqKey="Kinzler K">KW Kinzler</name>
</author>
<author><name sortKey="Vogelstein, B" uniqKey="Vogelstein B">B Vogelstein</name>
</author>
<author><name sortKey="Velculescu, Ve" uniqKey="Velculescu V">VE Velculescu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gopal, Ak" uniqKey="Gopal A">AK Gopal</name>
</author>
<author><name sortKey="Kahl, Bs" uniqKey="Kahl B">BS Kahl</name>
</author>
<author><name sortKey="De Vos, S" uniqKey="De Vos S">S de Vos</name>
</author>
<author><name sortKey="Wagner Johnston, Nd" uniqKey="Wagner Johnston N">ND Wagner-Johnston</name>
</author>
<author><name sortKey="Schuster, Sj" uniqKey="Schuster S">SJ Schuster</name>
</author>
<author><name sortKey="Jurczak, Wj" uniqKey="Jurczak W">WJ Jurczak</name>
</author>
<author><name sortKey="Flinn, Iw" uniqKey="Flinn I">IW Flinn</name>
</author>
<author><name sortKey="Flowers, Cr" uniqKey="Flowers C">CR Flowers</name>
</author>
<author><name sortKey="Martin, P" uniqKey="Martin P">P Martin</name>
</author>
<author><name sortKey="Viardot, A" uniqKey="Viardot A">A Viardot</name>
</author>
<author><name sortKey="Blum, Ka" uniqKey="Blum K">KA Blum</name>
</author>
<author><name sortKey="Goy, Ah" uniqKey="Goy A">AH Goy</name>
</author>
<author><name sortKey="Davies, Aj" uniqKey="Davies A">AJ Davies</name>
</author>
<author><name sortKey="Zinzani, Pl" uniqKey="Zinzani P">PL Zinzani</name>
</author>
<author><name sortKey="Dreyling, M" uniqKey="Dreyling M">M Dreyling</name>
</author>
<author><name sortKey="Johnson, D" uniqKey="Johnson D">D Johnson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Yaguchi, S" uniqKey="Yaguchi S">S Yaguchi</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Dan, S" uniqKey="Dan S">S Dan</name>
</author>
<author><name sortKey="Yamazaki, K" uniqKey="Yamazaki K">K Yamazaki</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Okamura, M" uniqKey="Okamura M">M Okamura</name>
</author>
<author><name sortKey="Yoshimi, H" uniqKey="Yoshimi H">H Yoshimi</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhao, W" uniqKey="Zhao W">W Zhao</name>
</author>
<author><name sortKey="Guo, W" uniqKey="Guo W">W Guo</name>
</author>
<author><name sortKey="Zhou, Q" uniqKey="Zhou Q">Q Zhou</name>
</author>
<author><name sortKey="Ma, Sn" uniqKey="Ma S">SN Ma</name>
</author>
<author><name sortKey="Wang, R" uniqKey="Wang R">R Wang</name>
</author>
<author><name sortKey="Qiu, Y" uniqKey="Qiu Y">Y Qiu</name>
</author>
<author><name sortKey="Jin, M" uniqKey="Jin M">M Jin</name>
</author>
<author><name sortKey="Duan, Hq" uniqKey="Duan H">HQ Duan</name>
</author>
<author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="O Brien, C" uniqKey="O Brien C">C O'Brien</name>
</author>
<author><name sortKey="Wallin, Jj" uniqKey="Wallin J">JJ Wallin</name>
</author>
<author><name sortKey="Sampath, D" uniqKey="Sampath D">D Sampath</name>
</author>
<author><name sortKey="Guhathakurta, D" uniqKey="Guhathakurta D">D GuhaThakurta</name>
</author>
<author><name sortKey="Savage, H" uniqKey="Savage H">H Savage</name>
</author>
<author><name sortKey="Punnoose, Ea" uniqKey="Punnoose E">EA Punnoose</name>
</author>
<author><name sortKey="Guan, J" uniqKey="Guan J">J Guan</name>
</author>
<author><name sortKey="Berry, L" uniqKey="Berry L">L Berry</name>
</author>
<author><name sortKey="Prior, Ww" uniqKey="Prior W">WW Prior</name>
</author>
<author><name sortKey="Amler, Lc" uniqKey="Amler L">LC Amler</name>
</author>
<author><name sortKey="Belvin, M" uniqKey="Belvin M">M Belvin</name>
</author>
<author><name sortKey="Friedman, Ls" uniqKey="Friedman L">LS Friedman</name>
</author>
<author><name sortKey="Lackner, Mr" uniqKey="Lackner M">MR Lackner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
<author><name sortKey="Yamazaki, K" uniqKey="Yamazaki K">K Yamazaki</name>
</author>
<author><name sortKey="Dan, S" uniqKey="Dan S">S Dan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Zj" uniqKey="Yang Z">ZJ Yang</name>
</author>
<author><name sortKey="Chee, Ce" uniqKey="Chee C">CE Chee</name>
</author>
<author><name sortKey="Huang, S" uniqKey="Huang S">S Huang</name>
</author>
<author><name sortKey="Sinicrope, Fa" uniqKey="Sinicrope F">FA Sinicrope</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pap, M" uniqKey="Pap M">M Pap</name>
</author>
<author><name sortKey="Cooper, Gm" uniqKey="Cooper G">GM Cooper</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mathew, R" uniqKey="Mathew R">R Mathew</name>
</author>
<author><name sortKey="Karantza Wadsworth, V" uniqKey="Karantza Wadsworth V">V Karantza-Wadsworth</name>
</author>
<author><name sortKey="White, E" uniqKey="White E">E White</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klionsky, Dj" uniqKey="Klionsky D">DJ Klionsky</name>
</author>
<author><name sortKey="Abdelmohsen, K" uniqKey="Abdelmohsen K">K Abdelmohsen</name>
</author>
<author><name sortKey="Abe, A" uniqKey="Abe A">A Abe</name>
</author>
<author><name sortKey="Abedin, Mj" uniqKey="Abedin M">MJ Abedin</name>
</author>
<author><name sortKey="Abeliovich, H" uniqKey="Abeliovich H">H Abeliovich</name>
</author>
<author><name sortKey="Acevedo Arozena, A" uniqKey="Acevedo Arozena A">A Acevedo Arozena</name>
</author>
<author><name sortKey="Adachi, H" uniqKey="Adachi H">H Adachi</name>
</author>
<author><name sortKey="Adams, Cm" uniqKey="Adams C">CM Adams</name>
</author>
<author><name sortKey="Adams, Pd" uniqKey="Adams P">PD Adams</name>
</author>
<author><name sortKey="Adeli, K" uniqKey="Adeli K">K Adeli</name>
</author>
<author><name sortKey="Adhihetty, Pj" uniqKey="Adhihetty P">PJ Adhihetty</name>
</author>
<author><name sortKey="Adler, Sg" uniqKey="Adler S">SG Adler</name>
</author>
<author><name sortKey="Agam, G" uniqKey="Agam G">G Agam</name>
</author>
<author><name sortKey="Agarwal, R" uniqKey="Agarwal R">R Agarwal</name>
</author>
<author><name sortKey="Aghi, Mk" uniqKey="Aghi M">MK Aghi</name>
</author>
<author><name sortKey="Agnello, M" uniqKey="Agnello M">M Agnello</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Funakoshi, T" uniqKey="Funakoshi T">T Funakoshi</name>
</author>
<author><name sortKey="Aki, T" uniqKey="Aki T">T Aki</name>
</author>
<author><name sortKey="Unuma, K" uniqKey="Unuma K">K Unuma</name>
</author>
<author><name sortKey="Uemura, K" uniqKey="Uemura K">K Uemura</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kimura, S" uniqKey="Kimura S">S Kimura</name>
</author>
<author><name sortKey="Noda, T" uniqKey="Noda T">T Noda</name>
</author>
<author><name sortKey="Yoshimori, T" uniqKey="Yoshimori T">T Yoshimori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moretti, L" uniqKey="Moretti L">L Moretti</name>
</author>
<author><name sortKey="Yang, Es" uniqKey="Yang E">ES Yang</name>
</author>
<author><name sortKey="Kim, Kw" uniqKey="Kim K">KW Kim</name>
</author>
<author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, Dx" uniqKey="Kong D">DX Kong</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takahashi Yanaga, F" uniqKey="Takahashi Yanaga F">F Takahashi-Yanaga</name>
</author>
<author><name sortKey="Sasaguri, T" uniqKey="Sasaguri T">T Sasaguri</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dan, S" uniqKey="Dan S">S Dan</name>
</author>
<author><name sortKey="Okamura, M" uniqKey="Okamura M">M Okamura</name>
</author>
<author><name sortKey="Mukai, Y" uniqKey="Mukai Y">Y Mukai</name>
</author>
<author><name sortKey="Yoshimi, H" uniqKey="Yoshimi H">H Yoshimi</name>
</author>
<author><name sortKey="Inoue, Y" uniqKey="Inoue Y">Y Inoue</name>
</author>
<author><name sortKey="Hanyu, A" uniqKey="Hanyu A">A Hanyu</name>
</author>
<author><name sortKey="Sakaue Sawano, A" uniqKey="Sakaue Sawano A">A Sakaue-Sawano</name>
</author>
<author><name sortKey="Imamura, T" uniqKey="Imamura T">T Imamura</name>
</author>
<author><name sortKey="Miyawaki, A" uniqKey="Miyawaki A">A Miyawaki</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dan, S" uniqKey="Dan S">S Dan</name>
</author>
<author><name sortKey="Yoshimi, H" uniqKey="Yoshimi H">H Yoshimi</name>
</author>
<author><name sortKey="Okamura, M" uniqKey="Okamura M">M Okamura</name>
</author>
<author><name sortKey="Mukai, Y" uniqKey="Mukai Y">Y Mukai</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Walczak, M" uniqKey="Walczak M">M Walczak</name>
</author>
<author><name sortKey="Martens, S" uniqKey="Martens S">S Martens</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bove, J" uniqKey="Bove J">J Bove</name>
</author>
<author><name sortKey="Martinez Vicente, M" uniqKey="Martinez Vicente M">M Martinez-Vicente</name>
</author>
<author><name sortKey="Vila, M" uniqKey="Vila M">M Vila</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jean, S" uniqKey="Jean S">S Jean</name>
</author>
<author><name sortKey="Kiger, Aa" uniqKey="Kiger A">AA Kiger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pasquier, B" uniqKey="Pasquier B">B Pasquier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Petiot, A" uniqKey="Petiot A">A Petiot</name>
</author>
<author><name sortKey="Ogier Denis, E" uniqKey="Ogier Denis E">E Ogier-Denis</name>
</author>
<author><name sortKey="Blommaart, Ef" uniqKey="Blommaart E">EF Blommaart</name>
</author>
<author><name sortKey="Meijer, Aj" uniqKey="Meijer A">AJ Meijer</name>
</author>
<author><name sortKey="Codogno, P" uniqKey="Codogno P">P Codogno</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kroemer, G" uniqKey="Kroemer G">G Kroemer</name>
</author>
<author><name sortKey="Galluzzi, L" uniqKey="Galluzzi L">L Galluzzi</name>
</author>
<author><name sortKey="Kepp, O" uniqKey="Kepp O">O Kepp</name>
</author>
<author><name sortKey="Zitvogel, L" uniqKey="Zitvogel L">L Zitvogel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Michaud, M" uniqKey="Michaud M">M Michaud</name>
</author>
<author><name sortKey="Martins, I" uniqKey="Martins I">I Martins</name>
</author>
<author><name sortKey="Sukkurwala, Aq" uniqKey="Sukkurwala A">AQ Sukkurwala</name>
</author>
<author><name sortKey="Adjemian, S" uniqKey="Adjemian S">S Adjemian</name>
</author>
<author><name sortKey="Ma, Y" uniqKey="Ma Y">Y Ma</name>
</author>
<author><name sortKey="Pellegatti, P" uniqKey="Pellegatti P">P Pellegatti</name>
</author>
<author><name sortKey="Shen, S" uniqKey="Shen S">S Shen</name>
</author>
<author><name sortKey="Kepp, O" uniqKey="Kepp O">O Kepp</name>
</author>
<author><name sortKey="Scoazec, M" uniqKey="Scoazec M">M Scoazec</name>
</author>
<author><name sortKey="Mignot, G" uniqKey="Mignot G">G Mignot</name>
</author>
<author><name sortKey="Rello Varona, S" uniqKey="Rello Varona S">S Rello-Varona</name>
</author>
<author><name sortKey="Tailler, M" uniqKey="Tailler M">M Tailler</name>
</author>
<author><name sortKey="Menger, L" uniqKey="Menger L">L Menger</name>
</author>
<author><name sortKey="Vacchelli, E" uniqKey="Vacchelli E">E Vacchelli</name>
</author>
<author><name sortKey="Galluzzi, L" uniqKey="Galluzzi L">L Galluzzi</name>
</author>
<author><name sortKey="Ghiringhelli, F" uniqKey="Ghiringhelli F">F Ghiringhelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Levenson, As" uniqKey="Levenson A">AS Levenson</name>
</author>
<author><name sortKey="Jordan, Vc" uniqKey="Jordan V">VC Jordan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cancer Genome Atlas, N" uniqKey="Cancer Genome Atlas N">N Cancer Genome Atlas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ma, Cx" uniqKey="Ma C">CX Ma</name>
</author>
<author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name>
</author>
<author><name sortKey="Naughton, M" uniqKey="Naughton M">M Naughton</name>
</author>
<author><name sortKey="Ademuyiwa, F" uniqKey="Ademuyiwa F">F Ademuyiwa</name>
</author>
<author><name sortKey="Suresh, R" uniqKey="Suresh R">R Suresh</name>
</author>
<author><name sortKey="Griffith, M" uniqKey="Griffith M">M Griffith</name>
</author>
<author><name sortKey="Griffith, Ol" uniqKey="Griffith O">OL Griffith</name>
</author>
<author><name sortKey="Skidmore, Zl" uniqKey="Skidmore Z">ZL Skidmore</name>
</author>
<author><name sortKey="Spies, Nc" uniqKey="Spies N">NC Spies</name>
</author>
<author><name sortKey="Ramu, A" uniqKey="Ramu A">A Ramu</name>
</author>
<author><name sortKey="Trani, L" uniqKey="Trani L">L Trani</name>
</author>
<author><name sortKey="Pluard, T" uniqKey="Pluard T">T Pluard</name>
</author>
<author><name sortKey="Nagaraj, G" uniqKey="Nagaraj G">G Nagaraj</name>
</author>
<author><name sortKey="Thomas, S" uniqKey="Thomas S">S Thomas</name>
</author>
<author><name sortKey="Guo, Z" uniqKey="Guo Z">Z Guo</name>
</author>
<author><name sortKey="Hoog, J" uniqKey="Hoog J">J Hoog</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mohamed, A" uniqKey="Mohamed A">A Mohamed</name>
</author>
<author><name sortKey="Krajewski, K" uniqKey="Krajewski K">K Krajewski</name>
</author>
<author><name sortKey="Cakar, B" uniqKey="Cakar B">B Cakar</name>
</author>
<author><name sortKey="Ma, Cx" uniqKey="Ma C">CX Ma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rodon, J" uniqKey="Rodon J">J Rodon</name>
</author>
<author><name sortKey="Brana, I" uniqKey="Brana I">I Brana</name>
</author>
<author><name sortKey="Siu, Ll" uniqKey="Siu L">LL Siu</name>
</author>
<author><name sortKey="De Jonge, Mj" uniqKey="De Jonge M">MJ De Jonge</name>
</author>
<author><name sortKey="Homji, N" uniqKey="Homji N">N Homji</name>
</author>
<author><name sortKey="Mills, D" uniqKey="Mills D">D Mills</name>
</author>
<author><name sortKey="Di Tomaso, E" uniqKey="Di Tomaso E">E Di Tomaso</name>
</author>
<author><name sortKey="Sarr, C" uniqKey="Sarr C">C Sarr</name>
</author>
<author><name sortKey="Trandafir, L" uniqKey="Trandafir L">L Trandafir</name>
</author>
<author><name sortKey="Massacesi, C" uniqKey="Massacesi C">C Massacesi</name>
</author>
<author><name sortKey="Eskens, F" uniqKey="Eskens F">F Eskens</name>
</author>
<author><name sortKey="Bendell, Jc" uniqKey="Bendell J">JC Bendell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name>
</author>
<author><name sortKey="Tang, Sa" uniqKey="Tang S">SA Tang</name>
</author>
<author><name sortKey="Lee, E" uniqKey="Lee E">E Lee</name>
</author>
<author><name sortKey="Qiu, Y" uniqKey="Qiu Y">Y Qiu</name>
</author>
<author><name sortKey="Wang, R" uniqKey="Wang R">R Wang</name>
</author>
<author><name sortKey="Duan, Hq" uniqKey="Duan H">HQ Duan</name>
</author>
<author><name sortKey="Dan, S" uniqKey="Dan S">S Dan</name>
</author>
<author><name sortKey="Jin, M" uniqKey="Jin M">M Jin</name>
</author>
<author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tang, Sa" uniqKey="Tang S">SA Tang</name>
</author>
<author><name sortKey="Zhou, Q" uniqKey="Zhou Q">Q Zhou</name>
</author>
<author><name sortKey="Guo, Wz" uniqKey="Guo W">WZ Guo</name>
</author>
<author><name sortKey="Qiu, Y" uniqKey="Qiu Y">Y Qiu</name>
</author>
<author><name sortKey="Wang, R" uniqKey="Wang R">R Wang</name>
</author>
<author><name sortKey="Jin, M" uniqKey="Jin M">M Jin</name>
</author>
<author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name>
</author>
<author><name sortKey="Li, K" uniqKey="Li K">K Li</name>
</author>
<author><name sortKey="Yamori, T" uniqKey="Yamori T">T Yamori</name>
</author>
<author><name sortKey="Dan, S" uniqKey="Dan S">S Dan</name>
</author>
<author><name sortKey="Kong, D" uniqKey="Kong D">D Kong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name>
</author>
<author><name sortKey="Luo, Q" uniqKey="Luo Q">Q Luo</name>
</author>
<author><name sortKey="Yuan, L" uniqKey="Yuan L">L Yuan</name>
</author>
<author><name sortKey="Miao, C" uniqKey="Miao C">C Miao</name>
</author>
<author><name sortKey="Mu, X" uniqKey="Mu X">X Mu</name>
</author>
<author><name sortKey="Xiao, W" uniqKey="Xiao W">W Xiao</name>
</author>
<author><name sortKey="Li, J" uniqKey="Li J">J Li</name>
</author>
<author><name sortKey="Sun, T" uniqKey="Sun T">T Sun</name>
</author>
<author><name sortKey="Ma, E" uniqKey="Ma E">E Ma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sheng, Y" uniqKey="Sheng Y">Y Sheng</name>
</author>
<author><name sortKey="Sun, B" uniqKey="Sun B">B Sun</name>
</author>
<author><name sortKey="Guo, Wt" uniqKey="Guo W">WT Guo</name>
</author>
<author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name>
</author>
<author><name sortKey="Wang, Yc" uniqKey="Wang Y">YC Wang</name>
</author>
<author><name sortKey="Xie, X" uniqKey="Xie X">X Xie</name>
</author>
<author><name sortKey="Xiao, Xl" uniqKey="Xiao X">XL Xiao</name>
</author>
<author><name sortKey="Li, N" uniqKey="Li N">N Li</name>
</author>
<author><name sortKey="Dong, Dl" uniqKey="Dong D">DL Dong</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Oncotarget</journal-id>
<journal-id journal-id-type="iso-abbrev">Oncotarget</journal-id>
<journal-id journal-id-type="publisher-id">Oncotarget</journal-id>
<journal-id journal-id-type="publisher-id">ImpactJ</journal-id>
<journal-title-group><journal-title>Oncotarget</journal-title>
</journal-title-group>
<issn pub-type="epub">1949-2553</issn>
<publisher><publisher-name>Impact Journals LLC</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26918351</article-id>
<article-id pub-id-type="pmc">4991426</article-id>
<article-id pub-id-type="publisher-id">7658</article-id>
<article-id pub-id-type="doi">10.18632/oncotarget.7658</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>ZSTK474, a specific class I phosphatidylinositol 3-kinase inhibitor, induces G1 arrest and autophagy in human breast cancer MCF-7 cells</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Yaochen</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="A2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Jing</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="A2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qiu</surname>
<given-names>Yuling</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jin</surname>
<given-names>Meihua</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Xi</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fan</surname>
<given-names>Guanwei</given-names>
</name>
<xref ref-type="aff" rid="A3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Ran</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kong</surname>
<given-names>Dexin</given-names>
</name>
<xref ref-type="aff" rid="A1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="A2"><sup>2</sup>
</xref>
</contrib>
</contrib-group>
<aff id="A1"><sup>1</sup>
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China</aff>
<aff id="A2"><sup>2</sup>
Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China</aff>
<aff id="A3"><sup>3</sup>
State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Nankai Dsitrict, Tianjin 300193, China</aff>
<author-notes><corresp id="cor1"><bold><italic>Correspondence to:</italic>
</bold>
<italic>Dexin Kong,</italic>
<email>kongdexin@tmu.edu.cn</email>
</corresp>
<corresp id="cor2"><italic>Ran Wang,</italic>
<email>wangran@tmu.edu.cn</email>
</corresp>
</author-notes>
<pub-date pub-type="collection"><day>12</day>
<month>4</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub"><day>24</day>
<month>2</month>
<year>2016</year>
</pub-date>
<volume>7</volume>
<issue>15</issue>
<fpage>19897</fpage>
<lpage>19909</lpage>
<history><date date-type="received"><day>10</day>
<month>11</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>16</day>
<month>2</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>Copyright: © 2016 Wang et al.</copyright-statement>
<copyright-year>2016</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.5/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<abstract><p>Multifaceted activities of class I phosphatidylinositol 3-kinase (PI3K) inhibitor ZSTK474 were investigated on human breast cancer cell MCF-7. ZSTK474 inhibited proliferation of MCF-7 cells potently. Flow cytometric analysis indicated that ZSTK474 induced cell cycle arrest at G1 phase, but no obvious apoptosis occurred. Western blot analysis suggested that blockade of PI3K/Akt/GSK-3β/cyclin D1/p-Rb pathway might contribute to the G1 arrest induced. Moreover, we demonstrated that ZSTK474 induced autophagy in MCF-7 cells by use of various assays including monodansylcadaverine (MDC) staining, transmission electron microscopy (TEM), tandem mRFP-GFP-LC3 fluorescence microscopy, and western blot detection of the autophagy protein markers of LC3B II, p62 and Atg 5. Inhibition of class I PI3K and the downstream mTOR might be involved in the autophagy-inducing effect. Combinational use of ZSTK474 and autophagy inhibitors enhanced cell viability, suggesting ZSTK474-induced autophagy might contribute to the antitumor activity. Our report supports the application of ZSTK474, which is being evaluated in clinical trials, for breast cancer therapy.</p>
</abstract>
<kwd-group><kwd>ZSTK474</kwd>
<kwd>PI3K inhibitor</kwd>
<kwd>MCF-7</kwd>
<kwd>G1 arrest</kwd>
<kwd>autophagy</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004258 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 004258 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:4991426 |texte= ZSTK474, a specific class I phosphatidylinositol 3-kinase inhibitor, induces G1 arrest and autophagy in human breast cancer MCF-7 cells }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:26918351" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
![]() | This area was generated with Dilib version V0.6.31. | ![]() |