Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging
Identifieur interne : 003E61 ( Pmc/Curation ); précédent : 003E60; suivant : 003E62Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging
Auteurs : Michael Weiler ; J. Brandon DixonSource :
- Frontiers in Physiology [ 1664-042X ] ; 2013.
Abstract
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DOI: 10.3389/fphys.2013.00215
PubMed: 23966950
PubMed Central: 3744037
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging</title><author><name sortKey="Weiler, Michael" sort="Weiler, Michael" uniqKey="Weiler M" first="Michael" last="Weiler">Michael Weiler</name></author><author><name sortKey="Dixon, J Brandon" sort="Dixon, J Brandon" uniqKey="Dixon J" first="J. Brandon" last="Dixon">J. Brandon Dixon</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23966950</idno><idno type="pmc">3744037</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744037</idno><idno type="RBID">PMC:3744037</idno><idno type="doi">10.3389/fphys.2013.00215</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">003E62</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003E62</idno><idno type="wicri:Area/Pmc/Curation">003E61</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003E61</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging</title><author><name sortKey="Weiler, Michael" sort="Weiler, Michael" uniqKey="Weiler M" first="Michael" last="Weiler">Michael Weiler</name></author><author><name sortKey="Dixon, J Brandon" sort="Dixon, J Brandon" uniqKey="Dixon J" first="J. Brandon" last="Dixon">J. Brandon Dixon</name></author></analytic><series><title level="j">Frontiers in Physiology</title><idno type="eISSN">1664-042X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><bold>Introduction:</bold> Near-infrared (NIR) imaging has emerged as a novel imaging modality for assessing lymphatic function <italic>in vivo</italic>. While the technique has provided quantitative data previously unavailable, questions remain in regards to the spatiotemporal capabilities of the approach. We address three of the more important issues here using the rodent tail, one of the most widely utilized <italic>in vivo</italic> model systems in the lymphatic literature. Specifically we demonstrate (1) the transient vs. steady state response of lymphatics to tracer injection, (2) the functional characteristics of multiple collecting vessels draining the same tissue space in parallel, and (3) the long-term consequences of fluorescent tracers on lymphatic function to repeated functional measurements.</p><p><bold>Methods</bold>: Rat tails were imaged with NIR and metrics of function were calculated for both collecting vessels that drain the tail. A nitric oxide donor cream (GTNO) was applied to the tail. Additionally, two different NIR dyes, indocyanine green (ICG) and LI-COR IRDye 800CW PEG, were utilized for function imaging at the time of initial injection and at 1, 2, and 4 week follow-up time points after which both draining lymph nodes were harvested.</p><p><bold>Results and Discussion</bold>: Significant differences were found between the two collecting vessels such that the vessel first showing fluorescence (dominant) produced enhanced functional metrics compared to the second vessel (non-dominant). GTNO significantly reduced lymphatic function in the non-dominant vessel compared to the dominant. ICG remained visible in the tail for 2 weeks after injection and was accompanied by significant losses in lymphatic function and enlarged draining lymph nodes. The Licor tracer also remained visible for 2 weeks. However, the dye produced significantly lower effects on lymphatic function than ICG, and lymph nodes were not enlarged at any time point, suggesting that this may be a more appropriate contrast agent for longitudinal lymphatic imaging.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Abels, C" uniqKey="Abels C">C. Abels</name></author><author><name sortKey="Fickweiler, S" uniqKey="Fickweiler S">S. Fickweiler</name></author><author><name sortKey="Weiderer, P" uniqKey="Weiderer P">P. Weiderer</name></author><author><name sortKey="Baumler, W" uniqKey="Baumler W">W. Baumler</name></author><author><name sortKey="Hofst Dter, F" uniqKey="Hofst Dter F">F. Hofstädter</name></author><author><name sortKey="Landthaler, M" uniqKey="Landthaler M">M. Landthaler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aldrich, M B" uniqKey="Aldrich M">M. B. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Front Physiol</journal-id><journal-id journal-id-type="publisher-id">Front. Physiol.</journal-id><journal-title-group><journal-title>Frontiers in Physiology</journal-title></journal-title-group><issn pub-type="epub">1664-042X</issn><publisher><publisher-name>Frontiers Media S.A.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23966950</article-id><article-id pub-id-type="pmc">3744037</article-id><article-id pub-id-type="doi">10.3389/fphys.2013.00215</article-id><article-categories><subj-group subj-group-type="heading"><subject>Physiology</subject><subj-group><subject>Original Research Article</subject></subj-group></subj-group></article-categories><title-group><article-title>Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Weiler</surname><given-names>Michael</given-names></name></contrib><contrib contrib-type="author"><name><surname>Dixon</surname><given-names>J. Brandon</given-names></name><xref ref-type="author-notes" rid="fn001"><sup>*</sup></xref></contrib></contrib-group><aff><institution>Wallace H. Coulter Department of Biomedical Engineering, George W. Woodruff School of Mechanical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology</institution><country>Atlanta, GA, USA</country></aff><author-notes><fn fn-type="edited-by"><p>Edited by: John D. Imig, Medical College of Wisconsin, USA</p></fn><fn fn-type="edited-by"><p>Reviewed by: Anatoliy A. Gashev, Texas A&M Health Science Center College of Medicine, USA; Katrin S. Blum, University Dusseldorf, Germany</p></fn><corresp id="fn001">*Correspondence: J. Brandon Dixon, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, IBB 2312, 315 Ferst Dr., Atlanta, GA 30332-0405, USA e-mail: <email xlink:type="simple">dixon@gatech.edu</email></corresp><fn fn-type="other" id="fn002"><p>This article was submitted to Frontiers in Vascular Physiology, a specialty of Frontiers in Physiology.</p></fn></author-notes><pub-date pub-type="epreprint"><day>07</day><month>7</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>15</day><month>8</month><year>2013</year></pub-date><pub-date pub-type="collection"><year>2013</year></pub-date><volume>4</volume><elocation-id>215</elocation-id><history><date date-type="received"><day>24</day><month>6</month><year>2013</year></date><date date-type="accepted"><day>27</day><month>7</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013 Weiler and Dixon.</copyright-statement><copyright-year>2013</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p></license></permissions><abstract><p><bold>Introduction:</bold> Near-infrared (NIR) imaging has emerged as a novel imaging modality for assessing lymphatic function <italic>in vivo</italic>. While the technique has provided quantitative data previously unavailable, questions remain in regards to the spatiotemporal capabilities of the approach. We address three of the more important issues here using the rodent tail, one of the most widely utilized <italic>in vivo</italic> model systems in the lymphatic literature. Specifically we demonstrate (1) the transient vs. steady state response of lymphatics to tracer injection, (2) the functional characteristics of multiple collecting vessels draining the same tissue space in parallel, and (3) the long-term consequences of fluorescent tracers on lymphatic function to repeated functional measurements.</p><p><bold>Methods</bold>: Rat tails were imaged with NIR and metrics of function were calculated for both collecting vessels that drain the tail. A nitric oxide donor cream (GTNO) was applied to the tail. Additionally, two different NIR dyes, indocyanine green (ICG) and LI-COR IRDye 800CW PEG, were utilized for function imaging at the time of initial injection and at 1, 2, and 4 week follow-up time points after which both draining lymph nodes were harvested.</p><p><bold>Results and Discussion</bold>: Significant differences were found between the two collecting vessels such that the vessel first showing fluorescence (dominant) produced enhanced functional metrics compared to the second vessel (non-dominant). GTNO significantly reduced lymphatic function in the non-dominant vessel compared to the dominant. ICG remained visible in the tail for 2 weeks after injection and was accompanied by significant losses in lymphatic function and enlarged draining lymph nodes. The Licor tracer also remained visible for 2 weeks. However, the dye produced significantly lower effects on lymphatic function than ICG, and lymph nodes were not enlarged at any time point, suggesting that this may be a more appropriate contrast agent for longitudinal lymphatic imaging.</p></abstract><kwd-group><kwd>indocyanine green</kwd><kwd>ICG</kwd><kwd>lymphatic</kwd><kwd>near-infrared imaging</kwd><kwd>lymphatic imaging</kwd><kwd>lymphatic function</kwd></kwd-group><counts><fig-count count="8"></fig-count><table-count count="1"></table-count><equation-count count="0"></equation-count><ref-count count="40"></ref-count><page-count count="10"></page-count><word-count count="7765"></word-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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