Serveur d'exploration sur le lymphœdème

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Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices

Identifieur interne : 003E44 ( Pmc/Curation ); précédent : 003E43; suivant : 003E45

Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices

Auteurs : Lea Knezevic [Autriche, Pays-Bas] ; Mira Schaupper [Autriche] ; Severin Mühleder [Autriche] ; Katharina Schimek [Allemagne] ; Tobias Hasenberg [Allemagne] ; Uwe Marx [Allemagne] ; Eleni Priglinger [Autriche] ; Heinz Redl [Autriche] ; Wolfgang Holnthoner [Autriche]

Source :

RBID : PMC:5394507

Abstract

Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of tissue-engineered constructs. The aim of this study was to isolate pure populations of lymphatic endothelial cells (LEC) and blood vascular endothelial cells (BEC) from human dermal microvascular endothelial cells and to study their network formation in our previously described coculture model with adipose-derived stromal cells (ASC) in fibrin scaffolds. We could follow the network development over a period of 4 weeks by fluorescently labeling the cells. We show that LEC and BEC form separate networks, which are morphologically distinguishable and sustainable over several weeks. In addition, lymphatic network development was dependent on vascular endothelial growth factor (VEGF)-C, resulting in denser networks with increasing VEGF-C concentration. Finally, we confirm the necessity of cell–cell contact between endothelial cells and ASC for the formation of both blood and lymphatic microvascular networks. This model represents a valuable platform for in vitro drug testing and for the future in vivo studies on lymphatic and blood microvascularization.


Url:
DOI: 10.3389/fbioe.2017.00025
PubMed: 28459049
PubMed Central: 5394507

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Links to Exploration step

PMC:5394507

Le document en format XML

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<name sortKey="Priglinger, Eleni" sort="Priglinger, Eleni" uniqKey="Priglinger E" first="Eleni" last="Priglinger">Eleni Priglinger</name>
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<institution>Ludwig Boltzmann Institute for Experimental and Clinical Traumatology</institution>
,
<addr-line>Vienna</addr-line>
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<p>Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of tissue-engineered constructs. The aim of this study was to isolate pure populations of lymphatic endothelial cells (LEC) and blood vascular endothelial cells (BEC) from human dermal microvascular endothelial cells and to study their network formation in our previously described coculture model with adipose-derived stromal cells (ASC) in fibrin scaffolds. We could follow the network development over a period of 4 weeks by fluorescently labeling the cells. We show that LEC and BEC form separate networks, which are morphologically distinguishable and sustainable over several weeks. In addition, lymphatic network development was dependent on vascular endothelial growth factor (VEGF)-C, resulting in denser networks with increasing VEGF-C concentration. Finally, we confirm the necessity of cell–cell contact between endothelial cells and ASC for the formation of both blood and lymphatic microvascular networks. This model represents a valuable platform for
<italic>in vitro</italic>
drug testing and for the future
<italic>in vivo</italic>
studies on lymphatic and blood microvascularization.</p>
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</TEI>
<pmc article-type="research-article">
<pmc-dir>properties open_access</pmc-dir>
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">Front Bioeng Biotechnol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Bioeng Biotechnol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Bioeng. Biotechnol.</journal-id>
<journal-title-group>
<journal-title>Frontiers in Bioengineering and Biotechnology</journal-title>
</journal-title-group>
<issn pub-type="epub">2296-4185</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="pmid">28459049</article-id>
<article-id pub-id-type="pmc">5394507</article-id>
<article-id pub-id-type="doi">10.3389/fbioe.2017.00025</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Bioengineering and Biotechnology</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Knezevic</surname>
<given-names>Lea</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="aff" rid="aff3">
<sup>3</sup>
</xref>
<xref ref-type="author-notes" rid="fn001">
<sup></sup>
</xref>
<uri xlink:type="simple" xlink:href="http://frontiersin.org/people/u/424464"></uri>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Schaupper</surname>
<given-names>Mira</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="fn001">
<sup></sup>
</xref>
<uri xlink:type="simple" xlink:href="http://frontiersin.org/people/u/415800"></uri>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Mühleder</surname>
<given-names>Severin</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://frontiersin.org/people/u/182298"></uri>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Schimek</surname>
<given-names>Katharina</given-names>
</name>
<xref ref-type="aff" rid="aff4">
<sup>4</sup>
</xref>
<xref ref-type="aff" rid="aff5">
<sup>5</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Hasenberg</surname>
<given-names>Tobias</given-names>
</name>
<xref ref-type="aff" rid="aff5">
<sup>5</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Marx</surname>
<given-names>Uwe</given-names>
</name>
<xref ref-type="aff" rid="aff5">
<sup>5</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Priglinger</surname>
<given-names>Eleni</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Redl</surname>
<given-names>Heinz</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://frontiersin.org/people/u/162134"></uri>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Holnthoner</surname>
<given-names>Wolfgang</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
<uri xlink:type="simple" xlink:href="http://frontiersin.org/people/u/167834"></uri>
</contrib>
</contrib-group>
<aff id="aff1">
<sup>1</sup>
<institution>Ludwig Boltzmann Institute for Experimental and Clinical Traumatology</institution>
,
<addr-line>Vienna</addr-line>
,
<country>Austria</country>
</aff>
<aff id="aff2">
<sup>2</sup>
<institution>Austrian Cluster for Tissue Regeneration</institution>
,
<addr-line>Vienna</addr-line>
,
<country>Austria</country>
</aff>
<aff id="aff3">
<sup>3</sup>
<institution>Department of Cardiology, Division of Heart and Lungs, University Medical Center Utrecht</institution>
,
<addr-line>Utrecht</addr-line>
,
<country>Netherlands</country>
</aff>
<aff id="aff4">
<sup>4</sup>
<institution>Technische Universität Berlin, Medical Biotechnology</institution>
,
<addr-line>Berlin</addr-line>
,
<country>Germany</country>
</aff>
<aff id="aff5">
<sup>5</sup>
<institution>TissUse GmbH</institution>
,
<addr-line>Berlin</addr-line>
,
<country>Germany</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>Edited by: Bruno Peault, University of California Los Angeles, USA</p>
</fn>
<fn fn-type="edited-by">
<p>Reviewed by: Andriana Margariti, Queen’s University Belfast, Ireland; Giovanni Vozzi, University of Pisa, Italy</p>
</fn>
<corresp content-type="corresp" id="cor1">*Correspondence: Wolfgang Holnthoner,
<email>wolfgang.holnthoner@trauma.lbg.ac.at</email>
</corresp>
<fn fn-type="other" id="fn001">
<p>
<sup></sup>
These authors have contributed equally to this work.</p>
</fn>
<fn fn-type="other" id="fn002">
<p>Specialty section: This article was submitted to Tissue Engineering and Regenerative Medicine, a section of the journal Frontiers in Bioengineering and Biotechnology</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>18</day>
<month>4</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="collection">
<year>2017</year>
</pub-date>
<volume>5</volume>
<elocation-id>25</elocation-id>
<history>
<date date-type="received">
<day>14</day>
<month>2</month>
<year>2017</year>
</date>
<date date-type="accepted">
<day>28</day>
<month>3</month>
<year>2017</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright © 2017 Knezevic, Schaupper, Mühleder, Schimek, Hasenberg, Marx, Priglinger, Redl and Holnthoner.</copyright-statement>
<copyright-year>2017</copyright-year>
<copyright-holder>Knezevic, Schaupper, Mühleder, Schimek, Hasenberg, Marx, Priglinger, Redl and Holnthoner</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract>
<p>Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are the main functions of the lymphatic system. Engineering both the blood and lymphatic microvasculature would advance the survival and functionality of tissue-engineered constructs. The aim of this study was to isolate pure populations of lymphatic endothelial cells (LEC) and blood vascular endothelial cells (BEC) from human dermal microvascular endothelial cells and to study their network formation in our previously described coculture model with adipose-derived stromal cells (ASC) in fibrin scaffolds. We could follow the network development over a period of 4 weeks by fluorescently labeling the cells. We show that LEC and BEC form separate networks, which are morphologically distinguishable and sustainable over several weeks. In addition, lymphatic network development was dependent on vascular endothelial growth factor (VEGF)-C, resulting in denser networks with increasing VEGF-C concentration. Finally, we confirm the necessity of cell–cell contact between endothelial cells and ASC for the formation of both blood and lymphatic microvascular networks. This model represents a valuable platform for
<italic>in vitro</italic>
drug testing and for the future
<italic>in vivo</italic>
studies on lymphatic and blood microvascularization.</p>
</abstract>
<kwd-group>
<kwd>lymphatics</kwd>
<kwd>endothelial cells</kwd>
<kwd>coculture</kwd>
<kwd>fibrin</kwd>
<kwd>tissue engineering</kwd>
</kwd-group>
<funding-group>
<award-group>
<funding-source id="cn01">Österreichische Forschungsförderungsgesellschaft
<named-content content-type="fundref-id">10.13039/501100004955</named-content>
</funding-source>
<award-id rid="cn01">FFG #845290</award-id>
</award-group>
</funding-group>
<counts>
<fig-count count="6"></fig-count>
<table-count count="0"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="64"></ref-count>
<page-count count="12"></page-count>
<word-count count="8522"></word-count>
</counts>
</article-meta>
</front>
</pmc>
</record>

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