Confocal Image-Based Computational Modeling of Nitric Oxide Transport in a Rat Mesenteric Lymphatic Vessel
Identifieur interne : 002E98 ( Pmc/Curation ); précédent : 002E97; suivant : 002E99Confocal Image-Based Computational Modeling of Nitric Oxide Transport in a Rat Mesenteric Lymphatic Vessel
Auteurs : John T. Wilson ; Wei Wang ; Augustus H. Hellerstedt ; David C. Zawieja ; James E. MooreSource :
- Journal of Biomechanical Engineering [ 0148-0731 ] ; 2013.
Abstract
The lymphatic system plays important roles in protein and solute transport as well as in the immune system. Its functionality is vital to proper homeostasis and fluid balance. Lymph may be propelled by intrinsic (active) vessel pumping or passive compression from external tissue movement. With regard to the former, nitric oxide (NO) is known to play an important role modulating lymphatic vessel contraction and vasodilation. Lymphatic endothelial cells (LECs) are sensitive to shear, and increases in flow have been shown to cause enhanced production of NO by LECs. Additionally, high concentrations of NO have been experimentally observed in the sinus region of mesenteric lymphatic vessels. A computational flow and mass transfer model using physiologic geometries obtained from confocal images of a rat mesenteric lymphatic vessel was developed to determine the characteristics of NO transport in the lymphatic flow regime. Both steady and unsteady analyses were performed. Production of NO was shear-dependent; basal cases using constant production were also generated. Simulations revealed areas of flow stagnation adjacent to the valve leaflets, suggesting the high concentrations observed here experimentally are due to minimal convection in this region. LEC sensitivity to shear was found to alter the concentration of NO in the vessel, and the convective forces were found to profoundly affect the concentration of NO at a Péclet value greater than approximately 61. The quasisteady analysis was able to resolve wall shear stress within 0.15% of the unsteady case. However, the percent difference between unsteady and quasisteady conditions was higher for NO concentration (6.7%). We have shown high NO concentrations adjacent to the valve leaflets are most likely due to flow-mediated processes rather than differential production by shear-sensitive LECs. Additionally, this model supports experimental findings of shear-dependent production, since removing shear dependence resulted in concentrations that are physiologically counterintuitive. Understanding the transport mechanisms and flow regimes in the lymphatic vasculature could help in the development of therapeutics to treat lymphatic disorders.
Url:
DOI: 10.1115/1.4023986
PubMed: 24231961
PubMed Central: 3707814
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002E99
Links to Exploration step
PMC:3707814Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Confocal Image-Based Computational Modeling of Nitric Oxide Transport in a Rat Mesenteric Lymphatic Vessel</title>
<author><name sortKey="Wilson, John T" sort="Wilson, John T" uniqKey="Wilson J" first="John T." last="Wilson">John T. Wilson</name>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
</author>
<author><name sortKey="Hellerstedt, Augustus H" sort="Hellerstedt, Augustus H" uniqKey="Hellerstedt A" first="Augustus H." last="Hellerstedt">Augustus H. Hellerstedt</name>
</author>
<author><name sortKey="Zawieja, David C" sort="Zawieja, David C" uniqKey="Zawieja D" first="David C." last="Zawieja">David C. Zawieja</name>
</author>
<author><name sortKey="Moore, James E" sort="Moore, James E" uniqKey="Moore J" first="James E." last="Moore">James E. Moore</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24231961</idno>
<idno type="pmc">3707814</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707814</idno>
<idno type="RBID">PMC:3707814</idno>
<idno type="doi">10.1115/1.4023986</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">002E99</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002E99</idno>
<idno type="wicri:Area/Pmc/Curation">002E98</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002E98</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Confocal Image-Based Computational Modeling of Nitric Oxide Transport in a Rat Mesenteric Lymphatic Vessel</title>
<author><name sortKey="Wilson, John T" sort="Wilson, John T" uniqKey="Wilson J" first="John T." last="Wilson">John T. Wilson</name>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
</author>
<author><name sortKey="Hellerstedt, Augustus H" sort="Hellerstedt, Augustus H" uniqKey="Hellerstedt A" first="Augustus H." last="Hellerstedt">Augustus H. Hellerstedt</name>
</author>
<author><name sortKey="Zawieja, David C" sort="Zawieja, David C" uniqKey="Zawieja D" first="David C." last="Zawieja">David C. Zawieja</name>
</author>
<author><name sortKey="Moore, James E" sort="Moore, James E" uniqKey="Moore J" first="James E." last="Moore">James E. Moore</name>
</author>
</analytic>
<series><title level="j">Journal of Biomechanical Engineering</title>
<idno type="ISSN">0148-0731</idno>
<idno type="eISSN">1528-8951</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The lymphatic system plays important roles in protein and solute transport as well as in the immune system. Its functionality is vital to proper homeostasis and fluid balance. Lymph may be propelled by intrinsic (active) vessel pumping or passive compression from external tissue movement. With regard to the former, nitric oxide (NO) is known to play an important role modulating lymphatic vessel contraction and vasodilation. Lymphatic endothelial cells (LECs) are sensitive to shear, and increases in flow have been shown to cause enhanced production of NO by LECs. Additionally, high concentrations of NO have been experimentally observed in the sinus region of mesenteric lymphatic vessels. A computational flow and mass transfer model using physiologic geometries obtained from confocal images of a rat mesenteric lymphatic vessel was developed to determine the characteristics of NO transport in the lymphatic flow regime. Both steady and unsteady analyses were performed. Production of NO was shear-dependent; basal cases using constant production were also generated. Simulations revealed areas of flow stagnation adjacent to the valve leaflets, suggesting the high concentrations observed here experimentally are due to minimal convection in this region. LEC sensitivity to shear was found to alter the concentration of NO in the vessel, and the convective forces were found to profoundly affect the concentration of NO at a Péclet value greater than approximately 61. The quasisteady analysis was able to resolve wall shear stress within 0.15% of the unsteady case. However, the percent difference between unsteady and quasisteady conditions was higher for NO concentration (6.7%). We have shown high NO concentrations adjacent to the valve leaflets are most likely due to flow-mediated processes rather than differential production by shear-sensitive LECs. Additionally, this model supports experimental findings of shear-dependent production, since removing shear dependence resulted in concentrations that are physiologically counterintuitive. Understanding the transport mechanisms and flow regimes in the lymphatic vasculature could help in the development of therapeutics to treat lymphatic disorders.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Biomech Eng</journal-id>
<journal-id journal-id-type="iso-abbrev">J Biomech Eng</journal-id>
<journal-id journal-id-type="pmc">BIO</journal-id>
<journal-title-group><journal-title>Journal of Biomechanical Engineering</journal-title>
</journal-title-group>
<issn pub-type="ppub">0148-0731</issn>
<issn pub-type="epub">1528-8951</issn>
<publisher><publisher-name>American Society of Mechanical Engineers</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24231961</article-id>
<article-id pub-id-type="pmc">3707814</article-id>
<article-id pub-id-type="doi">10.1115/1.4023986</article-id>
<article-id pub-id-type="coden">JBENDY</article-id>
<article-id pub-id-type="publisher-id">BIO-12-1570</article-id>
<article-id pub-id-type="publisher-manuscript">BIO-12-1570</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Papers</subject>
</subj-group>
</article-categories>
<title-group><article-title>Confocal Image-Based Computational Modeling of Nitric Oxide Transport in a Rat Mesenteric Lymphatic Vessel</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wilson</surname>
<given-names>John T.</given-names>
</name>
<aff><institution>Department of Bioengineering</institution>
,<break></break>
<institution>Imperial College London</institution>
,<break></break>
<addr-line>South Kensington Campus</addr-line>
,<break></break>
<addr-line>London SW7 2AZ</addr-line>
,<country>UK</country>
;<break></break>
Department of Biomedical Engineering,<break></break>
<institution>Texas A&M University</institution>
,<break></break>
<addr-line>5045 Emerging Technologies Building</addr-line>
,<break></break>
<addr-line>3120 TAMU</addr-line>
,<break></break>
<addr-line>College Station, TX 77843</addr-line>
</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Wei</given-names>
</name>
<aff><institution>Department of Systems Biology and Translational Medicine</institution>
,<break></break>
<institution>Texas A&M Health Science Center</institution>
,<break></break>
<addr-line>702 Southwest H.K. Dodgen Loop</addr-line>
,<break></break>
<addr-line>Temple, TX 76504</addr-line>
</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Hellerstedt</surname>
<given-names>Augustus H.</given-names>
</name>
<aff>Department of Biomedical Engineering,<break></break>
<institution>Texas A&M University</institution>
,<break></break>
<addr-line>5045 Emerging Technologies Building</addr-line>
,<break></break>
<addr-line>3120 TAMU</addr-line>
,<break></break>
<addr-line>College Station, TX 77843</addr-line>
</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Zawieja</surname>
<given-names>David C.</given-names>
</name>
<aff><institution>Department of Systems Biology and Translational Medicine</institution>
,<break></break>
<institution>Texas A&M Health Science Center</institution>
,<break></break>
<addr-line>702 Southwest H.K. Dodgen Loop</addr-line>
,<break></break>
<addr-line>Temple, TX 76504</addr-line>
</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Moore,</surname>
<given-names>James E.</given-names>
</name>
<role>Jr.</role>
<aff><institution>Department of Bioengineering</institution>
,<break></break>
<institution>Imperial College London</institution>
,<break></break>
<addr-line>South Kensington Campus</addr-line>
,<break></break>
<addr-line>London SW7 2AZ</addr-line>
,<country>UK</country>
;<break></break>
Department of Biomedical Engineering,<break></break>
<institution>Texas A&M University</institution>
,<break></break>
<addr-line>5045 Emerging Technologies Building</addr-line>
,<break></break>
<addr-line>3120 TAMU</addr-line>
,<break></break>
<addr-line>College Station, TX 77843</addr-line>
</aff>
</contrib>
</contrib-group>
<author-notes><fn fn-type="other"><p>Contributed by the Bioengineering Division of ASME for publication in the J<sc>OURNAL OF</sc>
B<sc>IOMECHANICAL</sc>
E<sc>NGINEERING</sc>
. Manuscript received November 19, 2012; final manuscript received March 4, 2013; accepted manuscript posted April 24, 2013; published online April 24, 2013. Assoc. Editor: Tim David.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>5</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>24</day>
<month>4</month>
<year>2013</year>
</pub-date>
<volume>135</volume>
<issue>5</issue>
<fpage>0510051</fpage>
<lpage>0510058</lpage>
<history><date date-type="received"><day>19</day>
<month>11</month>
<year>2012</year>
</date>
<date date-type="rev-recd"><day>04</day>
<month>3</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>08</day>
<month>3</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2013 by ASME</copyright-statement>
<copyright-year>2013</copyright-year>
<license license-type="ccc"><license-p>0148-0731/2013/135(5)/051005/8/<price>$0.00</price>
</license-p>
</license>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="bio_135_5_051005.pdf"></self-uri>
<abstract abstract-type="short"><p>The lymphatic system plays important roles in protein and solute transport as well as in the immune system. Its functionality is vital to proper homeostasis and fluid balance. Lymph may be propelled by intrinsic (active) vessel pumping or passive compression from external tissue movement. With regard to the former, nitric oxide (NO) is known to play an important role modulating lymphatic vessel contraction and vasodilation. Lymphatic endothelial cells (LECs) are sensitive to shear, and increases in flow have been shown to cause enhanced production of NO by LECs. Additionally, high concentrations of NO have been experimentally observed in the sinus region of mesenteric lymphatic vessels. A computational flow and mass transfer model using physiologic geometries obtained from confocal images of a rat mesenteric lymphatic vessel was developed to determine the characteristics of NO transport in the lymphatic flow regime. Both steady and unsteady analyses were performed. Production of NO was shear-dependent; basal cases using constant production were also generated. Simulations revealed areas of flow stagnation adjacent to the valve leaflets, suggesting the high concentrations observed here experimentally are due to minimal convection in this region. LEC sensitivity to shear was found to alter the concentration of NO in the vessel, and the convective forces were found to profoundly affect the concentration of NO at a Péclet value greater than approximately 61. The quasisteady analysis was able to resolve wall shear stress within 0.15% of the unsteady case. However, the percent difference between unsteady and quasisteady conditions was higher for NO concentration (6.7%). We have shown high NO concentrations adjacent to the valve leaflets are most likely due to flow-mediated processes rather than differential production by shear-sensitive LECs. Additionally, this model supports experimental findings of shear-dependent production, since removing shear dependence resulted in concentrations that are physiologically counterintuitive. Understanding the transport mechanisms and flow regimes in the lymphatic vasculature could help in the development of therapeutics to treat lymphatic disorders.</p>
</abstract>
<kwd-group kwd-group-type="author"><kwd>nitric oxide</kwd>
<kwd>computational fluid dynamics</kwd>
<kwd>mass transport</kwd>
<kwd>lymphatic</kwd>
</kwd-group>
<counts><fig-count count="10"></fig-count>
<table-count count="3"></table-count>
<equation-count count="9"></equation-count>
<ref-count count="19"></ref-count>
<page-count count="8"></page-count>
<word-count count="0000"></word-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E98 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 002E98 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:3707814 |texte= Confocal Image-Based Computational Modeling of Nitric Oxide Transport in a Rat Mesenteric Lymphatic Vessel }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:24231961" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
This area was generated with Dilib version V0.6.31. |