Neurotoxic effects of indocyanine green -cerebellar granule cell culture viability study
Identifieur interne : 002C54 ( Pmc/Curation ); précédent : 002C53; suivant : 002C55Neurotoxic effects of indocyanine green -cerebellar granule cell culture viability study
Auteurs : Beata Toczylowska [Pologne] ; Elzbieta Zieminska [Pologne] ; Grazyna Goch [Pologne] ; Daniel Milej [Pologne] ; Anna Gerega [Pologne] ; Adam Liebert [Pologne]Source :
- Biomedical Optics Express [ 2156-7085 ] ; 2014.
Abstract
The aim of this study was to examine neurotoxicity indocyanine green (ICG). We assessed viability of primary cerebellar granule cell culture (CGC) exposed to ICG to test two mechanisms that could be the first triggers causing neuronal toxicity: imbalance in calcium homeostasis and the degree of oligomerization of ICG molecules. We have observed this imbalance in CGC after exposure to 75-125μΜ ICG and dose and application sequence dependent protective effect of Gadovist on surviving neurons in vitro when used with ICG. Spectroscopic studies suggest the major cause of toxicity of the ICG is connected with oligomers formation. ICG at concentration of 25 μM (which is about 4 times higher than the highest concentration of ICG in the brain applied in in-vivo human studies) is not neurotoxic in the cell culture.
Url:
DOI: 10.1364/BOE.5.000800
PubMed: 24688815
PubMed Central: 3959834
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sort="Zieminska, Elzbieta" uniqKey="Zieminska E" first="Elzbieta" last="Zieminska">Elzbieta Zieminska</name><affiliation wicri:level="1"><nlm:aff id="aff3">Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Goch, Grazyna" sort="Goch, Grazyna" uniqKey="Goch G" first="Grazyna" last="Goch">Grazyna Goch</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Milej, Daniel" sort="Milej, Daniel" uniqKey="Milej D" first="Daniel" last="Milej">Daniel Milej</name><affiliation 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viability study</title><author><name sortKey="Toczylowska, Beata" sort="Toczylowska, Beata" uniqKey="Toczylowska B" first="Beata" last="Toczylowska">Beata Toczylowska</name><affiliation wicri:level="1"><nlm:aff id="aff1">Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zieminska, Elzbieta" sort="Zieminska, Elzbieta" uniqKey="Zieminska E" first="Elzbieta" last="Zieminska">Elzbieta Zieminska</name><affiliation wicri:level="1"><nlm:aff id="aff3">Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Goch, Grazyna" sort="Goch, Grazyna" uniqKey="Goch G" first="Grazyna" last="Goch">Grazyna Goch</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Milej, Daniel" sort="Milej, Daniel" uniqKey="Milej D" first="Daniel" last="Milej">Daniel Milej</name><affiliation wicri:level="1"><nlm:aff id="aff1">Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gerega, Anna" sort="Gerega, Anna" uniqKey="Gerega A" first="Anna" last="Gerega">Anna Gerega</name><affiliation wicri:level="1"><nlm:aff id="aff1">Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liebert, Adam" sort="Liebert, Adam" uniqKey="Liebert A" first="Adam" last="Liebert">Adam Liebert</name><affiliation wicri:level="1"><nlm:aff id="aff1">Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw,<country>Poland</country></nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biomedical Optics Express</title><idno type="eISSN">2156-7085</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aim of this study was to examine neurotoxicity indocyanine green (ICG). 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biomed Opt Express</journal-id><journal-id journal-id-type="iso-abbrev">Biomed Opt Express</journal-id><journal-id journal-id-type="publisher-id">BOE</journal-id><journal-title-group><journal-title>Biomedical Optics Express</journal-title></journal-title-group><issn pub-type="epub">2156-7085</issn><publisher><publisher-name>Optical Society of America</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24688815</article-id><article-id pub-id-type="pmc">3959834</article-id><article-id pub-id-type="publisher-id">203157</article-id><article-id pub-id-type="doi">10.1364/BOE.5.000800</article-id><article-categories><subj-group subj-group-type="heading"><subject>Molecular Imaging and Probe Development</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research-Article</subject></subj-group></article-categories><title-group><article-title>Neurotoxic effects of indocyanine green -cerebellar granule cell culture viability study</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Toczylowska</surname><given-names>Beata</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zieminska</surname><given-names>Elzbieta</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Goch</surname><given-names>Grazyna</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Milej</surname><given-names>Daniel</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gerega</surname><given-names>Anna</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liebert</surname><given-names>Adam</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><aff id="aff1"><label>1</label>Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw,<country>Poland</country></aff><aff id="aff2"><label>2</label>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></aff><aff id="aff3"><label>3</label>Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Str., 02-106 Warsaw,<country>Poland</country></aff></contrib-group><author-notes><corresp id="cor1"><label>*</label><email xlink:href="elziem@imdik.pan.pl">elziem@imdik.pan.pl</email></corresp></author-notes><pub-date pub-type="epub"><day>19</day><month>2</month><year>2014</year></pub-date><pub-date pub-type="collection"><day>01</day><month>3</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>19</day><month>2</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>5</volume><issue>3</issue><fpage>800</fpage><lpage>816</lpage><history><date date-type="received"><day>17</day><month>12</month><year>2013</year></date><date date-type="rev-recd"><day>08</day><month>2</month><year>2014</year></date><date date-type="accepted"><day>10</day><month>2</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 Optical Society of America</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Optical Society of America</copyright-holder></permissions><abstract><p>The aim of this study was to examine neurotoxicity indocyanine green (ICG). We assessed viability of primary cerebellar granule cell culture (CGC) exposed to ICG to test two mechanisms that could be the first triggers causing neuronal toxicity: imbalance in calcium homeostasis and the degree of oligomerization of ICG molecules. We have observed this imbalance in CGC after exposure to 75-125μΜ ICG and dose and application sequence dependent protective effect of Gadovist on surviving neurons in vitro when used with ICG. Spectroscopic studies suggest the major cause of toxicity of the ICG is connected with oligomers formation. ICG at concentration of 25 μM (which is about 4 times higher than the highest concentration of ICG in the brain applied in in-vivo human studies) is not neurotoxic in the cell culture.</p></abstract><kwd-group kwd-group-type="OCIS"><title>OCIS codes: </title><kwd>(170.0170) Medical optics and biotechnology</kwd><kwd>(170.1610) Clinical applications</kwd><kwd>(170.6280) Spectroscopy, fluorescence and luminescence</kwd><kwd>(170.1530) Cell analysis</kwd></kwd-group><funding-group><award-group><funding-source id="sp1">Polish National Science Center</funding-source><award-id rid="sp1">2011/03/B/ST7/02576</award-id></award-group></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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