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Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis

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Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis

Auteurs : B. Fischler ; P. Rodensjo ; A. Nemeth ; M. Forsgren ; I. Lewensohn-Fuchs

Source :

Archives of Disease in Childhood. Fetal and Neonatal Edition [ 1359-2998 ] ; 1999.

RBID : PMC:1720908

Abstract

AIM—To time the onset of cytomegalovirus (CMV) infection in patients (n=39) with CMV associated neonatal cholestasis by analysing CMV DNA on Guthrie cards sampled at 3 days of age. METHODS—CMV infection was diagnosed by serology/urine isolation or by CMV DNA detection (polymerase chain reaction) in liver biopsy specimens. In order to time the infection dry blood filter paper discs were punched out from stored Guthrie cards. After phenol-chloroform extraction CMV DNA was detected by nested polymerase chain reaction. RESULTS—All cards from control children (n=8) with congenital CMV tested positive; none of the negative controls (n=4) did so. Two of 39 cholestatic infants were CMV DNA positive; their mothers had serological signs compatible with infection during the second half of the pregnancy. All other cholestatic infants tested negative. CONCLUSIONS—CMV DNA was not detected in most of the children using Guthrie cards, suggesting that infection developed at or soon after birth.  

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1720908

PubMed: 10325791
PubMed Central: 1720908

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PMC:1720908

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cytomegalovirus DNA detection on Guthrie cards in patients with
neonatal cholestasis</title>
<author><name sortKey="Fischler, B" sort="Fischler, B" uniqKey="Fischler B" first="B." last="Fischler">B. Fischler</name>
</author>
<author><name sortKey="Rodensjo, P" sort="Rodensjo, P" uniqKey="Rodensjo P" first="P." last="Rodensjo">P. Rodensjo</name>
</author>
<author><name sortKey="Nemeth, A" sort="Nemeth, A" uniqKey="Nemeth A" first="A." last="Nemeth">A. Nemeth</name>
</author>
<author><name sortKey="Forsgren, M" sort="Forsgren, M" uniqKey="Forsgren M" first="M." last="Forsgren">M. Forsgren</name>
</author>
<author><name sortKey="Lewensohn Fuchs, I" sort="Lewensohn Fuchs, I" uniqKey="Lewensohn Fuchs I" first="I." last="Lewensohn-Fuchs">I. Lewensohn-Fuchs</name>
</author>
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<idno type="pmid">10325791</idno>
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<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1720908</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cytomegalovirus DNA detection on Guthrie cards in patients with
neonatal cholestasis</title>
<author><name sortKey="Fischler, B" sort="Fischler, B" uniqKey="Fischler B" first="B." last="Fischler">B. Fischler</name>
</author>
<author><name sortKey="Rodensjo, P" sort="Rodensjo, P" uniqKey="Rodensjo P" first="P." last="Rodensjo">P. Rodensjo</name>
</author>
<author><name sortKey="Nemeth, A" sort="Nemeth, A" uniqKey="Nemeth A" first="A." last="Nemeth">A. Nemeth</name>
</author>
<author><name sortKey="Forsgren, M" sort="Forsgren, M" uniqKey="Forsgren M" first="M." last="Forsgren">M. Forsgren</name>
</author>
<author><name sortKey="Lewensohn Fuchs, I" sort="Lewensohn Fuchs, I" uniqKey="Lewensohn Fuchs I" first="I." last="Lewensohn-Fuchs">I. Lewensohn-Fuchs</name>
</author>
</analytic>
<series><title level="j">Archives of Disease in Childhood. Fetal and Neonatal Edition</title>
<idno type="ISSN">1359-2998</idno>
<idno type="eISSN">1468-2052</idno>
<imprint><date when="1999">1999</date>
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<front><div type="abstract" xml:lang="en"><p><italic>AIM</italic>—To time the onset
of cytomegalovirus (CMV) infection in patients (n=39) with CMV
associated neonatal cholestasis by analysing CMV DNA on Guthrie cards
sampled at 3 days of age. <italic>METHODS</italic>—CMV infection
was diagnosed by serology/urine isolation or by CMV DNA detection
(polymerase chain reaction) in liver biopsy specimens. In order to time
the infection dry blood filter paper discs were punched out from stored
Guthrie cards. After phenol-chloroform extraction CMV DNA was detected
by nested polymerase chain reaction. <italic>RESULTS</italic>—All cards from
control children (n=8) with congenital CMV tested positive; none of the
negative controls (n=4) did so. Two of 39 cholestatic infants were CMV
DNA positive; their mothers had serological signs compatible with
infection during the second half of the pregnancy. All other
cholestatic infants tested negative. <italic>CONCLUSIONS</italic>—CMV DNA
was not detected in most of the children using Guthrie cards,
suggesting that infection developed at or soon after birth.  </p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Arch Dis Child Fetal Neonatal Ed</journal-id>
<journal-title>Archives of Disease in Childhood. Fetal and Neonatal Edition</journal-title>
<issn pub-type="ppub">1359-2998</issn>
<issn pub-type="epub">1468-2052</issn>
<publisher><publisher-name>BMJ Group</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">10325791</article-id>
<article-id pub-id-type="pmc">1720908</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cytomegalovirus DNA detection on Guthrie cards in patients with
neonatal cholestasis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Fischler</surname>
<given-names>B.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Rodensjo</surname>
<given-names>P.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Nemeth</surname>
<given-names>A.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Forsgren</surname>
<given-names>M.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Lewensohn-Fuchs</surname>
<given-names>I.</given-names>
</name>
</contrib>
</contrib-group>
<pub-date pub-type="ppub"><month>3</month>
<year>1999</year>
</pub-date>
<volume>80</volume>
<issue>2</issue>
<fpage>F130</fpage>
<lpage>F134</lpage>
<self-uri xlink:role="pdf" xlink:type="simple" xlink:href="http://fn.bmj.com/cgi/reprint/80/2/F130.pdf"></self-uri>
<self-uri xlink:role="abstract" xlink:type="simple" xlink:href="http://fn.bmj.com/cgi/content/abstract/80/2/F130"></self-uri>
<self-uri xlink:role="fulltext" xlink:type="simple" xlink:href="http://fn.bmj.com/cgi/content/full/80/2/F130"></self-uri>
<abstract><p><italic>AIM</italic>—To time the onset
of cytomegalovirus (CMV) infection in patients (n=39) with CMV
associated neonatal cholestasis by analysing CMV DNA on Guthrie cards
sampled at 3 days of age. <italic>METHODS</italic>—CMV infection
was diagnosed by serology/urine isolation or by CMV DNA detection
(polymerase chain reaction) in liver biopsy specimens. In order to time
the infection dry blood filter paper discs were punched out from stored
Guthrie cards. After phenol-chloroform extraction CMV DNA was detected
by nested polymerase chain reaction. <italic>RESULTS</italic>—All cards from
control children (n=8) with congenital CMV tested positive; none of the
negative controls (n=4) did so. Two of 39 cholestatic infants were CMV
DNA positive; their mothers had serological signs compatible with
infection during the second half of the pregnancy. All other
cholestatic infants tested negative. <italic>CONCLUSIONS</italic>—CMV DNA
was not detected in most of the children using Guthrie cards,
suggesting that infection developed at or soon after birth.  </p>
</abstract>
</article-meta>
</front>
</pmc>
</record>

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Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis

Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis

Source :

Abstract

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