IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype
Identifieur interne : 002A68 ( Pmc/Curation ); précédent : 002A67; suivant : 002A69IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype
Auteurs : P'Ng Loke [Royaume-Uni, États-Unis] ; Meera G. Nair [Royaume-Uni] ; John Parkinson [Royaume-Uni] ; David Guiliano [Royaume-Uni] ; Mark Blaxter [Royaume-Uni] ; Judith E. Allen [Royaume-Uni]Source :
- BMC Immunology [ 1471-2172 ] ; 2002.
Abstract
"Alternatively-activated" macrophages are found in Th2-mediated inflammatory settings such as nematode infection and allergic pulmonary inflammation. Due in part to a lack of markers, these cells have not been well characterized
We have used murine macrophages elicited by nematode infection (NeMφ) as a source of
Our data demonstrate that alternatively activated macrophages generated
Url:
DOI: 10.1186/1471-2172-3-7
PubMed: 12098359
PubMed Central: 117781
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype</title><author><name sortKey="Loke, P Ng" sort="Loke, P Ng" uniqKey="Loke P" first="P'Ng" last="Loke">P'Ng Loke</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</wicri:regionArea></affiliation></author><author><name sortKey="Nair, Meera G" sort="Nair, Meera G" uniqKey="Nair M" first="Meera G" last="Nair">Meera G. Nair</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Guiliano, David" sort="Guiliano, David" uniqKey="Guiliano D" first="David" last="Guiliano">David Guiliano</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Blaxter, Mark" sort="Blaxter, Mark" uniqKey="Blaxter M" first="Mark" last="Blaxter">Mark Blaxter</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Allen, Judith E" sort="Allen, Judith E" uniqKey="Allen J" first="Judith E" last="Allen">Judith E. Allen</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12098359</idno><idno type="pmc">117781</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117781</idno><idno type="RBID">PMC:117781</idno><idno type="doi">10.1186/1471-2172-3-7</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">002A69</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002A69</idno><idno type="wicri:Area/Pmc/Curation">002A68</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002A68</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype</title><author><name sortKey="Loke, P Ng" sort="Loke, P Ng" uniqKey="Loke P" first="P'Ng" last="Loke">P'Ng Loke</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720</wicri:regionArea></affiliation></author><author><name sortKey="Nair, Meera G" sort="Nair, Meera G" uniqKey="Nair M" first="Meera G" last="Nair">Meera G. Nair</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Guiliano, David" sort="Guiliano, David" uniqKey="Guiliano D" first="David" last="Guiliano">David Guiliano</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Blaxter, Mark" sort="Blaxter, Mark" uniqKey="Blaxter M" first="Mark" last="Blaxter">Mark Blaxter</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author><author><name sortKey="Allen, Judith E" sort="Allen, Judith E" uniqKey="Allen J" first="Judith E" last="Allen">Judith E. Allen</name><affiliation wicri:level="1"><nlm:aff id="I1">Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Immunology</title><idno type="eISSN">1471-2172</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>"Alternatively-activated" macrophages are found in Th2-mediated inflammatory settings such as nematode infection and allergic pulmonary inflammation. Due in part to a lack of markers, these cells have not been well characterized <italic>in vivo</italic> and their function remains unknown.</p></sec><sec><title>Results</title><p>We have used murine macrophages elicited by nematode infection (NeMφ) as a source of <italic>in vivo</italic> derived alternatively activated macrophages. Using three distinct yet complementary molecular approaches we have established a gene expression profile of alternatively activated macrophages and identified macrophage genes that are regulated <italic>in vivo</italic> by IL-4. First, genes abundantly expressed were identified by an expressed sequence tag strategy. Second, an array of 1176 known mouse genes was screened for differential expression between NeMφ from wild type or IL-4 deficient mice. Third, a subtractive library was screened to identify novel IL-4 dependent macrophage genes. Differential expression was confirmed by real time RT-PCR analysis.</p></sec><sec><title>Conclusions</title><p>Our data demonstrate that alternatively activated macrophages generated <italic>in vivo</italic> have a gene expression profile distinct from any macrophage population described to date. Several of the genes we identified, including those most abundantly expressed, have not previously been associated with macrophages and thus this study provides unique new information regarding the phenotype of macrophages found in Th2-mediated, chronic inflammatory settings. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Immunol</journal-id><journal-id journal-id-type="iso-abbrev">BMC Immunol</journal-id><journal-title-group><journal-title>BMC Immunology</journal-title></journal-title-group><issn pub-type="epub">1471-2172</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12098359</article-id><article-id pub-id-type="pmc">117781</article-id><article-id pub-id-type="publisher-id">1471-2172-3-7</article-id><article-id pub-id-type="doi">10.1186/1471-2172-3-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Loke</surname><given-names>P'ng</given-names></name><xref ref-type="aff" rid="I1">1</xref><xref ref-type="aff" rid="I2">2</xref><email>ploke@uclink.berkeley.edu</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Nair</surname><given-names>Meera G</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>m.g.nair@ed.ac.uk</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Parkinson</surname><given-names>John</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>john.parkinson@ed.ac.uk</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Guiliano</surname><given-names>David</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>David.Guiliano@ed.ac.uk</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Blaxter</surname><given-names>Mark</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>mark.blaxter@ed.ac.uk</email></contrib><contrib contrib-type="author" corresp="yes" id="A6"><name><surname>Allen</surname><given-names>Judith E</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>j.allen@ed.ac.uk</email></contrib></contrib-group><aff id="I1"><label>1</label>Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, UK</aff><aff id="I2"><label>2</label>Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA</aff><pub-date pub-type="collection"><year>2002</year></pub-date><pub-date pub-type="epub"><day>4</day><month>7</month><year>2002</year></pub-date><volume>3</volume><fpage>7</fpage><lpage>7</lpage><history><date date-type="received"><day>2</day><month>5</month><year>2002</year></date><date date-type="accepted"><day>4</day><month>7</month><year>2002</year></date></history><permissions><copyright-statement>Copyright ©2002 Loke et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</copyright-statement><copyright-year>2002</copyright-year><copyright-holder>Loke et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</copyright-holder></permissions><self-uri xlink:href="http://www.biomedcentral.com/1471-2172/3/7"></self-uri><abstract><sec><title>Background</title><p>"Alternatively-activated" macrophages are found in Th2-mediated inflammatory settings such as nematode infection and allergic pulmonary inflammation. Due in part to a lack of markers, these cells have not been well characterized <italic>in vivo</italic> and their function remains unknown.</p></sec><sec><title>Results</title><p>We have used murine macrophages elicited by nematode infection (NeMφ) as a source of <italic>in vivo</italic> derived alternatively activated macrophages. Using three distinct yet complementary molecular approaches we have established a gene expression profile of alternatively activated macrophages and identified macrophage genes that are regulated <italic>in vivo</italic> by IL-4. First, genes abundantly expressed were identified by an expressed sequence tag strategy. Second, an array of 1176 known mouse genes was screened for differential expression between NeMφ from wild type or IL-4 deficient mice. Third, a subtractive library was screened to identify novel IL-4 dependent macrophage genes. Differential expression was confirmed by real time RT-PCR analysis.</p></sec><sec><title>Conclusions</title><p>Our data demonstrate that alternatively activated macrophages generated <italic>in vivo</italic> have a gene expression profile distinct from any macrophage population described to date. Several of the genes we identified, including those most abundantly expressed, have not previously been associated with macrophages and thus this study provides unique new information regarding the phenotype of macrophages found in Th2-mediated, chronic inflammatory settings. Our data also provide additional <italic>in vivo</italic> evidence for parallels between the inflammatory processes involved in nematode infection and allergy.</p></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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