In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes
Identifieur interne : 002816 ( Pmc/Curation ); précédent : 002815; suivant : 002817In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes
Auteurs : Ekaterina I. Galanzha [États-Unis, Russie] ; Evgeny V. Shashkov [États-Unis, Russie] ; Valery V. Tuchin [Russie] ; Vladimir P. Zharov [États-Unis]Source :
- Cytometry. Part A : the journal of the International Society for Analytical Cytology [ 1552-4922 ] ; 2008.
Abstract
Compared to blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop
Cells in living animals were counted by laser (420-2300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data was verified by high-speed transmission, photothermal, and fluorescent imaging.
Counting melanoma and immune-related cells in normal, apoptotic, and necrotic stages in lymphatics
Multispectral, non-invasive, portable flow cytometery can be used for preclinical studies on animals with the potential of translation to humans for
Url:
DOI: 10.1002/cyto.a.20587
PubMed: 18677768
PubMed Central: 2606693
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<author><name sortKey="Galanzha, Ekaterina I" sort="Galanzha, Ekaterina I" uniqKey="Galanzha E" first="Ekaterina I." last="Galanzha">Ekaterina I. Galanzha</name>
<affiliation wicri:level="2"><nlm:aff id="A1">University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205</nlm:aff>
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<author><name sortKey="Galanzha, Ekaterina I" sort="Galanzha, Ekaterina I" uniqKey="Galanzha E" first="Ekaterina I." last="Galanzha">Ekaterina I. Galanzha</name>
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<author><name sortKey="Shashkov, Evgeny V" sort="Shashkov, Evgeny V" uniqKey="Shashkov E" first="Evgeny V." last="Shashkov">Evgeny V. Shashkov</name>
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<series><title level="j">Cytometry. Part A : the journal of the International Society for Analytical Cytology</title>
<idno type="ISSN">1552-4922</idno>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">Compared to blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop <italic>in vivo</italic>
lymph tests using the principles of flow cytometry.</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methods</title>
<p id="P2">Cells in living animals were counted by laser (420-2300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data was verified by high-speed transmission, photothermal, and fluorescent imaging.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Counting melanoma and immune-related cells in normal, apoptotic, and necrotic stages in lymphatics <italic>in vivo</italic>
was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of normal cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Multispectral, non-invasive, portable flow cytometery can be used for preclinical studies on animals with the potential of translation to humans for <italic>in vivo</italic>
PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra.</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101235694</journal-id>
<journal-id journal-id-type="pubmed-jr-id">32205</journal-id>
<journal-id journal-id-type="nlm-ta">Cytometry A</journal-id>
<journal-title>Cytometry. Part A : the journal of the International Society for Analytical Cytology</journal-title>
<issn pub-type="ppub">1552-4922</issn>
<issn pub-type="epub">1552-4930</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">18677768</article-id>
<article-id pub-id-type="pmc">2606693</article-id>
<article-id pub-id-type="doi">10.1002/cyto.a.20587</article-id>
<article-id pub-id-type="manuscript">NIHMS79944</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Galanzha</surname>
<given-names>Ekaterina I.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="aff" rid="A2">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shashkov</surname>
<given-names>Evgeny V.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="aff" rid="A3">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tuchin</surname>
<given-names>Valery V.</given-names>
</name>
<xref ref-type="aff" rid="A2">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zharov</surname>
<given-names>Vladimir P.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="corresp" rid="CR1">*</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>a</label>
University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205</aff>
<aff id="A2"><label>b</label>
Saratov State University, Institute of Optics and Biophotonics, Saratov 410012, Russia</aff>
<aff id="A3"><label>c</label>
Prokhorov General Physics Institute, Moscow 119991, Russia</aff>
<author-notes><corresp id="CR1"><label>*</label>
Correspondence to: Vladimir P. Zharov, PhD, Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, 4301 West Markham St. #543, Little Rock, AR 72205-7199, USA. Phone: 501-603-1213; fax: 501-686-8029 E-mail: <email>ZharovVladimirP@uams.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>4</day>
<month>12</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="ppub"><month>10</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>10</month>
<year>2009</year>
</pub-date>
<volume>73</volume>
<issue>10</issue>
<fpage>884</fpage>
<lpage>894</lpage>
<abstract><sec id="S1"><title>Background</title>
<p id="P1">Compared to blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop <italic>in vivo</italic>
lymph tests using the principles of flow cytometry.</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methods</title>
<p id="P2">Cells in living animals were counted by laser (420-2300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data was verified by high-speed transmission, photothermal, and fluorescent imaging.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Counting melanoma and immune-related cells in normal, apoptotic, and necrotic stages in lymphatics <italic>in vivo</italic>
was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of normal cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Multispectral, non-invasive, portable flow cytometery can be used for preclinical studies on animals with the potential of translation to humans for <italic>in vivo</italic>
PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra.</p>
</sec>
</abstract>
<kwd-group><kwd><italic>in vitro</italic>
flow cytometry</kwd>
<kwd>lymphatics</kwd>
<kwd>sentinel lymph nodes</kwd>
<kwd>photoacoustic method</kwd>
<kwd>multicolor detection</kwd>
<kwd>nanoparticles</kwd>
<kwd>melanoma metastasis</kwd>
<kwd>immunity</kwd>
<kwd>apoptosis</kwd>
<kwd>necrosis</kwd>
</kwd-group>
<contract-num rid="EB1">R01 EB000873-04
||EB</contract-num>
<contract-sponsor id="EB1">National Institute of Biomedical Imaging and Bioengineering : NIBIB</contract-sponsor>
</article-meta>
</front>
</pmc>
</record>
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