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In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes

Identifieur interne : 002816 ( Pmc/Curation ); précédent : 002815; suivant : 002817

In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes

Auteurs : Ekaterina I. Galanzha [États-Unis, Russie] ; Evgeny V. Shashkov [États-Unis, Russie] ; Valery V. Tuchin [Russie] ; Vladimir P. Zharov [États-Unis]

Source :

Cytometry. Part A : the journal of the International Society for Analytical Cytology [ 1552-4922 ] ; 2008.

RBID : PMC:2606693

Abstract

Background

Compared to blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop in vivo lymph tests using the principles of flow cytometry.

Methods

Cells in living animals were counted by laser (420-2300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data was verified by high-speed transmission, photothermal, and fluorescent imaging.

Results

Counting melanoma and immune-related cells in normal, apoptotic, and necrotic stages in lymphatics in vivo was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of normal cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays.

Conclusions

Multispectral, non-invasive, portable flow cytometery can be used for preclinical studies on animals with the potential of translation to humans for in vivo PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606693

DOI: 10.1002/cyto.a.20587
PubMed: 18677768
PubMed Central: 2606693

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<author><name sortKey="Galanzha, Ekaterina I" sort="Galanzha, Ekaterina I" uniqKey="Galanzha E" first="Ekaterina I." last="Galanzha">Ekaterina I. Galanzha</name>
<affiliation wicri:level="2"><nlm:aff id="A1">University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Arkansas</region>
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<wicri:cityArea>University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock</wicri:cityArea>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">Compared to blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop <italic>in vivo</italic>
 lymph tests using the principles of flow cytometry.</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methods</title>
<p id="P2">Cells in living animals were counted by laser (420-2300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data was verified by high-speed transmission, photothermal, and fluorescent imaging.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Counting melanoma and immune-related cells in normal, apoptotic, and necrotic stages in lymphatics <italic>in vivo</italic>
 was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of normal cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Multispectral, non-invasive, portable flow cytometery can be used for preclinical studies on animals with the potential of translation to humans for <italic>in vivo</italic>
 PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">101235694</journal-id>
<journal-id journal-id-type="pubmed-jr-id">32205</journal-id>
<journal-id journal-id-type="nlm-ta">Cytometry A</journal-id>
<journal-title>Cytometry. Part A : the journal of the International Society for Analytical Cytology</journal-title>
<issn pub-type="ppub">1552-4922</issn>
<issn pub-type="epub">1552-4930</issn>
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<article-meta><article-id pub-id-type="pmid">18677768</article-id>
<article-id pub-id-type="pmc">2606693</article-id>
<article-id pub-id-type="doi">10.1002/cyto.a.20587</article-id>
<article-id pub-id-type="manuscript">NIHMS79944</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Galanzha</surname>
<given-names>Ekaterina I.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="aff" rid="A2">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shashkov</surname>
<given-names>Evgeny V.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="aff" rid="A3">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tuchin</surname>
<given-names>Valery V.</given-names>
</name>
<xref ref-type="aff" rid="A2">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zharov</surname>
<given-names>Vladimir P.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
<xref ref-type="corresp" rid="CR1">*</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>a</label>
University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205</aff>
<aff id="A2"><label>b</label>
Saratov State University, Institute of Optics and Biophotonics, Saratov 410012, Russia</aff>
<aff id="A3"><label>c</label>
Prokhorov General Physics Institute, Moscow 119991, Russia</aff>
<author-notes><corresp id="CR1"><label>*</label>
Correspondence to: Vladimir P. Zharov, PhD, Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, 4301 West Markham St. #543, Little Rock, AR 72205-7199, USA. Phone: 501-603-1213; fax: 501-686-8029 E-mail: <email>ZharovVladimirP@uams.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>4</day>
<month>12</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="ppub"><month>10</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>10</month>
<year>2009</year>
</pub-date>
<volume>73</volume>
<issue>10</issue>
<fpage>884</fpage>
<lpage>894</lpage>
<abstract><sec id="S1"><title>Background</title>
<p id="P1">Compared to blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop <italic>in vivo</italic>
 lymph tests using the principles of flow cytometry.</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methods</title>
<p id="P2">Cells in living animals were counted by laser (420-2300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data was verified by high-speed transmission, photothermal, and fluorescent imaging.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Counting melanoma and immune-related cells in normal, apoptotic, and necrotic stages in lymphatics <italic>in vivo</italic>
 was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of normal cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Multispectral, non-invasive, portable flow cytometery can be used for preclinical studies on animals with the potential of translation to humans for <italic>in vivo</italic>
 PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra.</p>
</sec>
</abstract>
<kwd-group><kwd><italic>in vitro</italic>
 flow cytometry</kwd>
<kwd>lymphatics</kwd>
<kwd>sentinel lymph nodes</kwd>
<kwd>photoacoustic method</kwd>
<kwd>multicolor detection</kwd>
<kwd>nanoparticles</kwd>
<kwd>melanoma metastasis</kwd>
<kwd>immunity</kwd>
<kwd>apoptosis</kwd>
<kwd>necrosis</kwd>
</kwd-group>
<contract-num rid="EB1">R01 EB000873-04
				||EB</contract-num>
<contract-sponsor id="EB1">National Institute of Biomedical Imaging and Bioengineering  : NIBIB</contract-sponsor>
</article-meta>
</front>
</pmc>
</record>

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In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes

In Vivo Multispectral Photoacoustic Lymph Flow Cytometry with Natural Cell Focusing and Multicolor Nanoparticle Probes

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