Lymphatic vessel density and function in experimental bladder cancer
Identifieur interne : 001B02 ( Pmc/Curation ); précédent : 001B01; suivant : 001B03Lymphatic vessel density and function in experimental bladder cancer
Auteurs : Marcia R. Saban [États-Unis] ; Rheal Towner [États-Unis] ; Nataliya Smith [États-Unis] ; Andrew Abbott [États-Unis] ; Michal Neeman [Israël] ; Carole A. Davis [États-Unis] ; Cindy Simpson [États-Unis] ; Julie Maier [États-Unis] ; Sylvie Mémet [France] ; Xue-Ru Wu [États-Unis] ; Ricardo Saban [États-Unis]Source :
- BMC Cancer [ 1471-2407 ] ; 2007.
Abstract
The lymphatics form a second circulatory system that drains the extracellular fluid and proteins from the tumor microenvironment, and provides an exclusive environment in which immune cells interact and respond to foreign antigen. Both cancer and inflammation are known to induce lymphangiogenesis. However, little is known about bladder lymphatic vessels and their involvement in cancer formation and progression.
A double transgenic mouse model was generated by crossing a bladder cancer-induced transgenic, in which SV40 large T antigen was under the control of uroplakin II promoter, with another transgenic mouse harboring a
SV40-
SV40-
Url:
DOI: 10.1186/1471-2407-7-219
PubMed: 18047671
PubMed Central: 2241841
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001B03
Links to Exploration step
PMC:2241841Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic vessel density and function in experimental bladder cancer</title>
<author><name sortKey="Saban, Marcia R" sort="Saban, Marcia R" uniqKey="Saban M" first="Marcia R" last="Saban">Marcia R. Saban</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Towner, Rheal" sort="Towner, Rheal" uniqKey="Towner R" first="Rheal" last="Towner">Rheal Towner</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Smith, Nataliya" sort="Smith, Nataliya" uniqKey="Smith N" first="Nataliya" last="Smith">Nataliya Smith</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Abbott, Andrew" sort="Abbott, Andrew" uniqKey="Abbott A" first="Andrew" last="Abbott">Andrew Abbott</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Neeman, Michal" sort="Neeman, Michal" uniqKey="Neeman M" first="Michal" last="Neeman">Michal Neeman</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel</nlm:aff>
<country xml:lang="fr">Israël</country>
<wicri:regionArea>Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Davis, Carole A" sort="Davis, Carole A" uniqKey="Davis C" first="Carole A" last="Davis">Carole A. Davis</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Simpson, Cindy" sort="Simpson, Cindy" uniqKey="Simpson C" first="Cindy" last="Simpson">Cindy Simpson</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Maier, Julie" sort="Maier, Julie" uniqKey="Maier J" first="Julie" last="Maier">Julie Maier</name>
<affiliation wicri:level="1"><nlm:aff id="I4">Oklahoma Medical Research Foundation (OMRF), Imaging Core Facility, Oklahoma City, Oklahoma 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Oklahoma Medical Research Foundation (OMRF), Imaging Core Facility, Oklahoma City, Oklahoma 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Memet, Sylvie" sort="Memet, Sylvie" uniqKey="Memet S" first="Sylvie" last="Mémet">Sylvie Mémet</name>
<affiliation wicri:level="1"><nlm:aff id="I5">Unité de Mycologie Moléculaire, URA CNRS 3012, Institut Pasteur, 75724 Paris Cedex 15, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Unité de Mycologie Moléculaire, URA CNRS 3012, Institut Pasteur, 75724 Paris Cedex 15</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wu, Xue Ru" sort="Wu, Xue Ru" uniqKey="Wu X" first="Xue-Ru" last="Wu">Xue-Ru Wu</name>
<affiliation wicri:level="1"><nlm:aff id="I6">Department of Urology, New York University Medical School, New York, NY 10016, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Urology, New York University Medical School, New York, NY 10016</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Saban, Ricardo" sort="Saban, Ricardo" uniqKey="Saban R" first="Ricardo" last="Saban">Ricardo Saban</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">18047671</idno>
<idno type="pmc">2241841</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241841</idno>
<idno type="RBID">PMC:2241841</idno>
<idno type="doi">10.1186/1471-2407-7-219</idno>
<date when="2007">2007</date>
<idno type="wicri:Area/Pmc/Corpus">001B03</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001B03</idno>
<idno type="wicri:Area/Pmc/Curation">001B02</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001B02</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Lymphatic vessel density and function in experimental bladder cancer</title>
<author><name sortKey="Saban, Marcia R" sort="Saban, Marcia R" uniqKey="Saban M" first="Marcia R" last="Saban">Marcia R. Saban</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Towner, Rheal" sort="Towner, Rheal" uniqKey="Towner R" first="Rheal" last="Towner">Rheal Towner</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Smith, Nataliya" sort="Smith, Nataliya" uniqKey="Smith N" first="Nataliya" last="Smith">Nataliya Smith</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Abbott, Andrew" sort="Abbott, Andrew" uniqKey="Abbott A" first="Andrew" last="Abbott">Andrew Abbott</name>
<affiliation wicri:level="1"><nlm:aff id="I2">Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Neeman, Michal" sort="Neeman, Michal" uniqKey="Neeman M" first="Michal" last="Neeman">Michal Neeman</name>
<affiliation wicri:level="1"><nlm:aff id="I3">Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel</nlm:aff>
<country xml:lang="fr">Israël</country>
<wicri:regionArea>Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Davis, Carole A" sort="Davis, Carole A" uniqKey="Davis C" first="Carole A" last="Davis">Carole A. Davis</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Simpson, Cindy" sort="Simpson, Cindy" uniqKey="Simpson C" first="Cindy" last="Simpson">Cindy Simpson</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Maier, Julie" sort="Maier, Julie" uniqKey="Maier J" first="Julie" last="Maier">Julie Maier</name>
<affiliation wicri:level="1"><nlm:aff id="I4">Oklahoma Medical Research Foundation (OMRF), Imaging Core Facility, Oklahoma City, Oklahoma 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Oklahoma Medical Research Foundation (OMRF), Imaging Core Facility, Oklahoma City, Oklahoma 73104</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Memet, Sylvie" sort="Memet, Sylvie" uniqKey="Memet S" first="Sylvie" last="Mémet">Sylvie Mémet</name>
<affiliation wicri:level="1"><nlm:aff id="I5">Unité de Mycologie Moléculaire, URA CNRS 3012, Institut Pasteur, 75724 Paris Cedex 15, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Unité de Mycologie Moléculaire, URA CNRS 3012, Institut Pasteur, 75724 Paris Cedex 15</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wu, Xue Ru" sort="Wu, Xue Ru" uniqKey="Wu X" first="Xue-Ru" last="Wu">Xue-Ru Wu</name>
<affiliation wicri:level="1"><nlm:aff id="I6">Department of Urology, New York University Medical School, New York, NY 10016, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Urology, New York University Medical School, New York, NY 10016</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Saban, Ricardo" sort="Saban, Ricardo" uniqKey="Saban R" first="Ricardo" last="Saban">Ricardo Saban</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Cancer</title>
<idno type="eISSN">1471-2407</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The lymphatics form a second circulatory system that drains the extracellular fluid and proteins from the tumor microenvironment, and provides an exclusive environment in which immune cells interact and respond to foreign antigen. Both cancer and inflammation are known to induce lymphangiogenesis. However, little is known about bladder lymphatic vessels and their involvement in cancer formation and progression.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>A double transgenic mouse model was generated by crossing a bladder cancer-induced transgenic, in which SV40 large T antigen was under the control of uroplakin II promoter, with another transgenic mouse harboring a <italic>lacZ </italic>
reporter gene under the control of an NF-κB-responsive promoter (κB-<italic>lacZ</italic>
) exhibiting constitutive activity of β-galactosidase in lymphatic endothelial cells. In this new mouse model (SV40-<italic>lacZ</italic>
), we examined the lymphatic vessel density (LVD) and function (LVF) during bladder cancer progression. LVD was performed in bladder whole mounts and cross-sections by fluorescent immunohistochemistry (IHC) using LYVE-1 antibody. LVF was assessed by real-time <italic>in vivo </italic>
imaging techniques using a contrast agent (biotin-BSA-Gd-DTPA-Cy5.5; Gd-Cy5.5) suitable for both magnetic resonance imaging (MRI) and near infrared fluorescence (NIRF). In addition, IHC of Cy5.5 was used for time-course analysis of co-localization of Gd-Cy5.5 with LYVE-1-positive lymphatics and CD31-positive blood vessels.</p>
</sec>
<sec><title>Results</title>
<p>SV40-<italic>lacZ </italic>
mice develop bladder cancer and permitted visualization of lymphatics. A significant increase in LVD was found concomitantly with bladder cancer progression. Double labeling of the bladder cross-sections with LYVE-1 and Ki-67 antibodies indicated cancer-induced lymphangiogenesis. MRI detected mouse bladder cancer, as early as 4 months, and permitted to follow tumor sizes during cancer progression. Using Gd-Cy5.5 as a contrast agent for MRI-guided lymphangiography, we determined a possible reduction of lymphatic flow within the tumoral area. In addition, NIRF studies of Gd-Cy5.5 confirmed its temporal distribution between CD31-positive blood vessels and LYVE-1 positive lymphatic vessels.</p>
</sec>
<sec><title>Conclusion</title>
<p>SV40-<italic>lacZ </italic>
mice permit the visualization of lymphatics during bladder cancer progression. Gd-Cy5.5, as a double contrast agent for NIRF and MRI, permits to quantify delivery, transport rates, and volumes of macromolecular fluid flow through the interstitial-lymphatic continuum. Our results open the path for the study of lymphatic activity <italic>in vivo </italic>
and in real time, and support the role of lymphangiogenesis during bladder cancer progression.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Cancer</journal-id>
<journal-title>BMC Cancer</journal-title>
<issn pub-type="epub">1471-2407</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">18047671</article-id>
<article-id pub-id-type="pmc">2241841</article-id>
<article-id pub-id-type="publisher-id">1471-2407-7-219</article-id>
<article-id pub-id-type="doi">10.1186/1471-2407-7-219</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Lymphatic vessel density and function in experimental bladder cancer</article-title>
</title-group>
<contrib-group><contrib id="A1" corresp="yes" contrib-type="author"><name><surname>Saban</surname>
<given-names>Marcia R</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>marcia-saban@ouhsc.edu</email>
</contrib>
<contrib id="A2" contrib-type="author"><name><surname>Towner</surname>
<given-names>Rheal</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>rheal-towner@omrf.ouhsc.edu</email>
</contrib>
<contrib id="A3" contrib-type="author"><name><surname>Smith</surname>
<given-names>Nataliya</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>Nataliya-Smith@omrf.ouhsc.edu</email>
</contrib>
<contrib id="A4" contrib-type="author"><name><surname>Abbott</surname>
<given-names>Andrew</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>andrew-abbott@omrf.ouhsc.edu</email>
</contrib>
<contrib id="A5" contrib-type="author"><name><surname>Neeman</surname>
<given-names>Michal</given-names>
</name>
<xref ref-type="aff" rid="I3">3</xref>
<email>michal.neeman@weizmann.ac.il</email>
</contrib>
<contrib id="A6" contrib-type="author"><name><surname>Davis</surname>
<given-names>Carole A</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>carole-davis@ouhsc.edu</email>
</contrib>
<contrib id="A7" contrib-type="author"><name><surname>Simpson</surname>
<given-names>Cindy</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>cindy-simpson@ouhsc.edu</email>
</contrib>
<contrib id="A8" contrib-type="author"><name><surname>Maier</surname>
<given-names>Julie</given-names>
</name>
<xref ref-type="aff" rid="I4">4</xref>
<email>maierj@omrf.ouhsc.edu</email>
</contrib>
<contrib id="A9" contrib-type="author"><name><surname>Mémet</surname>
<given-names>Sylvie</given-names>
</name>
<xref ref-type="aff" rid="I5">5</xref>
<email>symemet@pasteur.fr</email>
</contrib>
<contrib id="A10" contrib-type="author"><name><surname>Wu</surname>
<given-names>Xue-Ru</given-names>
</name>
<xref ref-type="aff" rid="I6">6</xref>
<email>wux01@med.nyu.edu</email>
</contrib>
<contrib id="A11" contrib-type="author"><name><surname>Saban</surname>
<given-names>Ricardo</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>ricardo-saban@ouhsc.edu</email>
</contrib>
</contrib-group>
<aff id="I1"><label>1</label>
Department of Physiology, College of Medicine, Oklahoma University Health Sciences Center (OUHSC), Oklahoma City, OK 73104, USA</aff>
<aff id="I2"><label>2</label>
Small Animal MRI Core Facility, Free Radical Biology & Aging, Oklahoma Medical Research Foundation (OMRF), Oklahoma City, OK 73104, USA</aff>
<aff id="I3"><label>3</label>
Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel</aff>
<aff id="I4"><label>4</label>
Oklahoma Medical Research Foundation (OMRF), Imaging Core Facility, Oklahoma City, Oklahoma 73104, USA</aff>
<aff id="I5"><label>5</label>
Unité de Mycologie Moléculaire, URA CNRS 3012, Institut Pasteur, 75724 Paris Cedex 15, France</aff>
<aff id="I6"><label>6</label>
Department of Urology, New York University Medical School, New York, NY 10016, USA</aff>
<pub-date pub-type="collection"><year>2007</year>
</pub-date>
<pub-date pub-type="epub"><day>29</day>
<month>11</month>
<year>2007</year>
</pub-date>
<volume>7</volume>
<fpage>219</fpage>
<lpage>219</lpage>
<ext-link ext-link-type="uri" xlink:href="http://www.biomedcentral.com/1471-2407/7/219"></ext-link>
<history><date date-type="received"><day>28</day>
<month>9</month>
<year>2007</year>
</date>
<date date-type="accepted"><day>29</day>
<month>11</month>
<year>2007</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2007 Saban et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2007</copyright-year>
<copyright-holder>Saban et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
<pmc-comment>
Saban
R
Marcia
marcia-saban@ouhsc.edu
Lymphatic vessel density and function in experimental bladder cancer
2007 BMC Cancer 7(1): 219-. (2007) 1471-2407(2007)7:1<219> urn:ISSN:1471-2407 </pmc-comment>
</license>
</permissions>
<abstract><sec><title>Background</title>
<p>The lymphatics form a second circulatory system that drains the extracellular fluid and proteins from the tumor microenvironment, and provides an exclusive environment in which immune cells interact and respond to foreign antigen. Both cancer and inflammation are known to induce lymphangiogenesis. However, little is known about bladder lymphatic vessels and their involvement in cancer formation and progression.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>A double transgenic mouse model was generated by crossing a bladder cancer-induced transgenic, in which SV40 large T antigen was under the control of uroplakin II promoter, with another transgenic mouse harboring a <italic>lacZ </italic>
reporter gene under the control of an NF-κB-responsive promoter (κB-<italic>lacZ</italic>
) exhibiting constitutive activity of β-galactosidase in lymphatic endothelial cells. In this new mouse model (SV40-<italic>lacZ</italic>
), we examined the lymphatic vessel density (LVD) and function (LVF) during bladder cancer progression. LVD was performed in bladder whole mounts and cross-sections by fluorescent immunohistochemistry (IHC) using LYVE-1 antibody. LVF was assessed by real-time <italic>in vivo </italic>
imaging techniques using a contrast agent (biotin-BSA-Gd-DTPA-Cy5.5; Gd-Cy5.5) suitable for both magnetic resonance imaging (MRI) and near infrared fluorescence (NIRF). In addition, IHC of Cy5.5 was used for time-course analysis of co-localization of Gd-Cy5.5 with LYVE-1-positive lymphatics and CD31-positive blood vessels.</p>
</sec>
<sec><title>Results</title>
<p>SV40-<italic>lacZ </italic>
mice develop bladder cancer and permitted visualization of lymphatics. A significant increase in LVD was found concomitantly with bladder cancer progression. Double labeling of the bladder cross-sections with LYVE-1 and Ki-67 antibodies indicated cancer-induced lymphangiogenesis. MRI detected mouse bladder cancer, as early as 4 months, and permitted to follow tumor sizes during cancer progression. Using Gd-Cy5.5 as a contrast agent for MRI-guided lymphangiography, we determined a possible reduction of lymphatic flow within the tumoral area. In addition, NIRF studies of Gd-Cy5.5 confirmed its temporal distribution between CD31-positive blood vessels and LYVE-1 positive lymphatic vessels.</p>
</sec>
<sec><title>Conclusion</title>
<p>SV40-<italic>lacZ </italic>
mice permit the visualization of lymphatics during bladder cancer progression. Gd-Cy5.5, as a double contrast agent for NIRF and MRI, permits to quantify delivery, transport rates, and volumes of macromolecular fluid flow through the interstitial-lymphatic continuum. Our results open the path for the study of lymphatic activity <italic>in vivo </italic>
and in real time, and support the role of lymphangiogenesis during bladder cancer progression.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B02 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 001B02 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:2241841 |texte= Lymphatic vessel density and function in experimental bladder cancer }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:18047671" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
![]() | This area was generated with Dilib version V0.6.31. | ![]() |