Molecular biomarkers screened by next-generation RNA sequencing for non-sentinel lymph node status prediction in breast cancer patients with metastatic sentinel lymph nodes
Identifieur interne : 000E98 ( Pmc/Curation ); précédent : 000E97; suivant : 000E99Molecular biomarkers screened by next-generation RNA sequencing for non-sentinel lymph node status prediction in breast cancer patients with metastatic sentinel lymph nodes
Auteurs : Feng Liang [République populaire de Chine] ; Hongzhu Qu [République populaire de Chine] ; Qiang Lin [République populaire de Chine] ; Yadong Yang [République populaire de Chine] ; Xiuyan Ruan [République populaire de Chine] ; Bo Zhang [République populaire de Chine] ; Yi Liu [République populaire de Chine] ; Chengze Yu [République populaire de Chine] ; Hongyan Zhang [République populaire de Chine] ; Xiangdong Fang [République populaire de Chine] ; Xiaopeng Hao [République populaire de Chine]Source :
- World Journal of Surgical Oncology [ 1477-7819 ] ; 2015.
Abstract
Non-sentinel lymph node (NSLN) status prediction with molecular biomarkers may make some sentinel lymph node (SLN) positive breast cancer patients avoid the axillary lymph node dissection, but the available markers remain limited.
SLN positive patients with and without NSLN invasion were selected, and genes differentially expressed or fused in SLN metastasis were screened by next-generation RNA sequencing.
Six candidates (all ER/PR+, HER2−, Ki-67 <20 %) with metastatic SLNs selected from 305 patients were equally categorized as NSLN negative and positive. We identified 103 specifically expressed genes in the NSLN negative group and 47 in the NSLN positive group. Among them,
The biomarkers screened in present study may broaden our understanding of the mechanisms of breast cancer metastasis to the lymph nodes and contribute to the axillary surgery selection for SLN positive patients.
The online version of this article (doi:10.1186/s12957-015-0642-2) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12957-015-0642-2
PubMed: 26311227
PubMed Central: 4551378
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000E99
Links to Exploration step
PMC:4551378Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular biomarkers screened by next-generation RNA sequencing for non-sentinel lymph node status prediction in breast cancer patients with metastatic sentinel lymph nodes</title>
<author><name sortKey="Liang, Feng" sort="Liang, Feng" uniqKey="Liang F" first="Feng" last="Liang">Feng Liang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Qu, Hongzhu" sort="Qu, Hongzhu" uniqKey="Qu H" first="Hongzhu" last="Qu">Hongzhu Qu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lin, Qiang" sort="Lin, Qiang" uniqKey="Lin Q" first="Qiang" last="Lin">Qiang Lin</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yang, Yadong" sort="Yang, Yadong" uniqKey="Yang Y" first="Yadong" last="Yang">Yadong Yang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ruan, Xiuyan" sort="Ruan, Xiuyan" uniqKey="Ruan X" first="Xiuyan" last="Ruan">Xiuyan Ruan</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Yi" sort="Liu, Yi" uniqKey="Liu Y" first="Yi" last="Liu">Yi Liu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yu, Chengze" sort="Yu, Chengze" uniqKey="Yu C" first="Chengze" last="Yu">Chengze Yu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Hongyan" sort="Zhang, Hongyan" uniqKey="Zhang H" first="Hongyan" last="Zhang">Hongyan Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3">General Hospital of Beijing Military Area, 5 Nanmencang, Dongcheng District, Beijing, 100700 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>General Hospital of Beijing Military Area, 5 Nanmencang, Dongcheng District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fang, Xiangdong" sort="Fang, Xiangdong" uniqKey="Fang X" first="Xiangdong" last="Fang">Xiangdong Fang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hao, Xiaopeng" sort="Hao, Xiaopeng" uniqKey="Hao X" first="Xiaopeng" last="Hao">Xiaopeng Hao</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26311227</idno>
<idno type="pmc">4551378</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551378</idno>
<idno type="RBID">PMC:4551378</idno>
<idno type="doi">10.1186/s12957-015-0642-2</idno>
<date when="2015">2015</date>
<idno type="wicri:Area/Pmc/Corpus">000E99</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E99</idno>
<idno type="wicri:Area/Pmc/Curation">000E98</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E98</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular biomarkers screened by next-generation RNA sequencing for non-sentinel lymph node status prediction in breast cancer patients with metastatic sentinel lymph nodes</title>
<author><name sortKey="Liang, Feng" sort="Liang, Feng" uniqKey="Liang F" first="Feng" last="Liang">Feng Liang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Qu, Hongzhu" sort="Qu, Hongzhu" uniqKey="Qu H" first="Hongzhu" last="Qu">Hongzhu Qu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lin, Qiang" sort="Lin, Qiang" uniqKey="Lin Q" first="Qiang" last="Lin">Qiang Lin</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yang, Yadong" sort="Yang, Yadong" uniqKey="Yang Y" first="Yadong" last="Yang">Yadong Yang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ruan, Xiuyan" sort="Ruan, Xiuyan" uniqKey="Ruan X" first="Xiuyan" last="Ruan">Xiuyan Ruan</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Liu, Yi" sort="Liu, Yi" uniqKey="Liu Y" first="Yi" last="Liu">Yi Liu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yu, Chengze" sort="Yu, Chengze" uniqKey="Yu C" first="Chengze" last="Yu">Chengze Yu</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhang, Hongyan" sort="Zhang, Hongyan" uniqKey="Zhang H" first="Hongyan" last="Zhang">Hongyan Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3">General Hospital of Beijing Military Area, 5 Nanmencang, Dongcheng District, Beijing, 100700 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>General Hospital of Beijing Military Area, 5 Nanmencang, Dongcheng District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fang, Xiangdong" sort="Fang, Xiangdong" uniqKey="Fang X" first="Xiangdong" last="Fang">Xiangdong Fang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hao, Xiaopeng" sort="Hao, Xiaopeng" uniqKey="Hao X" first="Xiaopeng" last="Hao">Xiaopeng Hao</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">World Journal of Surgical Oncology</title>
<idno type="eISSN">1477-7819</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Non-sentinel lymph node (NSLN) status prediction with molecular biomarkers may make some sentinel lymph node (SLN) positive breast cancer patients avoid the axillary lymph node dissection, but the available markers remain limited.</p>
</sec>
<sec><title>Methods</title>
<p>SLN positive patients with and without NSLN invasion were selected, and genes differentially expressed or fused in SLN metastasis were screened by next-generation RNA sequencing.</p>
</sec>
<sec><title>Results</title>
<p>Six candidates (all ER/PR+, HER2−, Ki-67 <20 %) with metastatic SLNs selected from 305 patients were equally categorized as NSLN negative and positive. We identified 103 specifically expressed genes in the NSLN negative group and 47 in the NSLN positive group. Among them, <italic>FABP1</italic>
(negative group) and <italic>CYP2A13</italic>
(positive group) were the only 2 protein-encoding genes with expression levels in the 8th to 10th deciles. Using a false discovery rate threshold of <0.05, 62 up-regulated genes and 98 down-regulated genes were discovered in the NSLN positive group. Furthermore, 10 gene fusions were identified in this group with the most frequently fused gene being <italic>IGLL5</italic>
.</p>
</sec>
<sec><title>Conclusions</title>
<p>The biomarkers screened in present study may broaden our understanding of the mechanisms of breast cancer metastasis to the lymph nodes and contribute to the axillary surgery selection for SLN positive patients.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12957-015-0642-2) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Halsted, Ws" uniqKey="Halsted W">WS Halsted</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="D Ngelo Donovan, Dd" uniqKey="D Ngelo Donovan D">DD D’Angelo-Donovan</name>
</author>
<author><name sortKey="Dickson Witmer, D" uniqKey="Dickson Witmer D">D Dickson-Witmer</name>
</author>
<author><name sortKey="Petrelli, Nj" uniqKey="Petrelli N">NJ Petrelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jafferbhoy, S" uniqKey="Jafferbhoy S">S Jafferbhoy</name>
</author>
<author><name sortKey="Mcwilliams, B" uniqKey="Mcwilliams B">B McWilliams</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Jj" uniqKey="Chen J">JJ Chen</name>
</author>
<author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Zee, Kj" uniqKey="Van Zee K">KJ Van Zee</name>
</author>
<author><name sortKey="Manasseh, Dm" uniqKey="Manasseh D">DM Manasseh</name>
</author>
<author><name sortKey="Bevilacqua, Jl" uniqKey="Bevilacqua J">JL Bevilacqua</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Trapnell, C" uniqKey="Trapnell C">C Trapnell</name>
</author>
<author><name sortKey="Pachter, L" uniqKey="Pachter L">L Pachter</name>
</author>
<author><name sortKey="Salzberg, Sl" uniqKey="Salzberg S">SL Salzberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Da Huang, W" uniqKey="Da Huang W">W da Huang</name>
</author>
<author><name sortKey="Sherman, Bt" uniqKey="Sherman B">BT Sherman</name>
</author>
<author><name sortKey="Lempicki, Ra" uniqKey="Lempicki R">RA Lempicki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, D" uniqKey="Kim D">D Kim</name>
</author>
<author><name sortKey="Salzberg, Sl" uniqKey="Salzberg S">SL Salzberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Avgeris, M" uniqKey="Avgeris M">M Avgeris</name>
</author>
<author><name sortKey="Mavridis, K" uniqKey="Mavridis K">K Mavridis</name>
</author>
<author><name sortKey="Scorilas, A" uniqKey="Scorilas A">A Scorilas</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Gauld, Sb" uniqKey="Gauld S">SB Gauld</name>
</author>
<author><name sortKey="Dal Porto, Jm" uniqKey="Dal Porto J">JM Dal Porto</name>
</author>
<author><name sortKey="Cambier, Jc" uniqKey="Cambier J">JC Cambier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Robak, T" uniqKey="Robak T">T Robak</name>
</author>
<author><name sortKey="Robak, P" uniqKey="Robak P">P Robak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thumser, Ae" uniqKey="Thumser A">AE Thumser</name>
</author>
<author><name sortKey="Moore, Jb" uniqKey="Moore J">JB Moore</name>
</author>
<author><name sortKey="Plant, Nj" uniqKey="Plant N">NJ Plant</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sun, L" uniqKey="Sun L">L Sun</name>
</author>
<author><name sortKey="Fan, X" uniqKey="Fan X">X Fan</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">World J Surg Oncol</journal-id>
<journal-id journal-id-type="iso-abbrev">World J Surg Oncol</journal-id>
<journal-title-group><journal-title>World Journal of Surgical Oncology</journal-title>
</journal-title-group>
<issn pub-type="epub">1477-7819</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26311227</article-id>
<article-id pub-id-type="pmc">4551378</article-id>
<article-id pub-id-type="publisher-id">642</article-id>
<article-id pub-id-type="doi">10.1186/s12957-015-0642-2</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Molecular biomarkers screened by next-generation RNA sequencing for non-sentinel lymph node status prediction in breast cancer patients with metastatic sentinel lymph nodes</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Liang</surname>
<given-names>Feng</given-names>
</name>
<address><email>lfpeakcool@126.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Qu</surname>
<given-names>Hongzhu</given-names>
</name>
<address><email>quhongzhu@big.ac.cn</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Lin</surname>
<given-names>Qiang</given-names>
</name>
<address><email>812983585@qq.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Yadong</given-names>
</name>
<address><email>yangyd@big.ac.cn</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ruan</surname>
<given-names>Xiuyan</given-names>
</name>
<address><email>ruanxy@big.ac.cn</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Bo</given-names>
</name>
<address><email>13436660521@qq.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Yi</given-names>
</name>
<address><email>liuyi790114@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yu</surname>
<given-names>Chengze</given-names>
</name>
<address><email>yuchengze@sina.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Zhang</surname>
<given-names>Hongyan</given-names>
</name>
<address><phone>+86 1084008502</phone>
<email>roninzhy@126.com</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Fang</surname>
<given-names>Xiangdong</given-names>
</name>
<address><phone>+86 1084097495</phone>
<email>fangxd@big.ac.cn</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Hao</surname>
<given-names>Xiaopeng</given-names>
</name>
<address><phone>+86 1066947382</phone>
<email>hxp307@sina.com</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1"><label></label>
Affiliated Hospital of Academy of Military Medical Sciences, 8 Dongdajie, Fengtai District, Beijing, 100071 China</aff>
<aff id="Aff2"><label></label>
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 1-104 Beichen West Road, Chaoyang District, Beijing, 100101 China</aff>
<aff id="Aff3"><label></label>
General Hospital of Beijing Military Area, 5 Nanmencang, Dongcheng District, Beijing, 100700 China</aff>
</contrib-group>
<pub-date pub-type="epub"><day>28</day>
<month>8</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>28</day>
<month>8</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><year>2015</year>
</pub-date>
<volume>13</volume>
<elocation-id>258</elocation-id>
<history><date date-type="received"><day>27</day>
<month>5</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>3</day>
<month>7</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© Liang et al. 2015</copyright-statement>
<license license-type="OpenAccess"><license-p>Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>Non-sentinel lymph node (NSLN) status prediction with molecular biomarkers may make some sentinel lymph node (SLN) positive breast cancer patients avoid the axillary lymph node dissection, but the available markers remain limited.</p>
</sec>
<sec><title>Methods</title>
<p>SLN positive patients with and without NSLN invasion were selected, and genes differentially expressed or fused in SLN metastasis were screened by next-generation RNA sequencing.</p>
</sec>
<sec><title>Results</title>
<p>Six candidates (all ER/PR+, HER2−, Ki-67 <20 %) with metastatic SLNs selected from 305 patients were equally categorized as NSLN negative and positive. We identified 103 specifically expressed genes in the NSLN negative group and 47 in the NSLN positive group. Among them, <italic>FABP1</italic>
(negative group) and <italic>CYP2A13</italic>
(positive group) were the only 2 protein-encoding genes with expression levels in the 8th to 10th deciles. Using a false discovery rate threshold of <0.05, 62 up-regulated genes and 98 down-regulated genes were discovered in the NSLN positive group. Furthermore, 10 gene fusions were identified in this group with the most frequently fused gene being <italic>IGLL5</italic>
.</p>
</sec>
<sec><title>Conclusions</title>
<p>The biomarkers screened in present study may broaden our understanding of the mechanisms of breast cancer metastasis to the lymph nodes and contribute to the axillary surgery selection for SLN positive patients.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12957-015-0642-2) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Breast cancer</kwd>
<kwd>Sentinel lymph node (SLN)</kwd>
<kwd>Non-sentinel lymph node (NSLN)</kwd>
<kwd>Axillary lymph node dissection (ALND)</kwd>
<kwd>RNA sequencing</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2015</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E98 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000E98 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:4551378 |texte= Molecular biomarkers screened by next-generation RNA sequencing for non-sentinel lymph node status prediction in breast cancer patients with metastatic sentinel lymph nodes }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:26311227" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
![]() | This area was generated with Dilib version V0.6.31. | ![]() |