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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Modified Collagen Gel Dressing Promotes Angiogenesis in a Pre-Clinical Swine Model of Chronic Ischemic Wounds</title><author><name sortKey="Elgharably, Haytham" sort="Elgharably, Haytham" uniqKey="Elgharably H" first="Haytham" last="Elgharably">Haytham Elgharably</name></author><author><name sortKey="Ganesh, Kasturi" sort="Ganesh, Kasturi" uniqKey="Ganesh K" first="Kasturi" last="Ganesh">Kasturi Ganesh</name></author><author><name sortKey="Dickerson, Jennifer" sort="Dickerson, Jennifer" uniqKey="Dickerson J" first="Jennifer" last="Dickerson">Jennifer Dickerson</name></author><author><name sortKey="Khanna, Savita" sort="Khanna, Savita" uniqKey="Khanna S" first="Savita" last="Khanna">Savita Khanna</name></author><author><name sortKey="Abas, Motaz" sort="Abas, Motaz" uniqKey="Abas M" first="Motaz" last="Abas">Motaz Abas</name></author><author><name sortKey="Ghatak, Piya Das" sort="Ghatak, Piya Das" uniqKey="Ghatak P" first="Piya Das" last="Ghatak">Piya Das Ghatak</name></author><author><name sortKey="Dixit, Sriteja" sort="Dixit, Sriteja" uniqKey="Dixit S" first="Sriteja" last="Dixit">Sriteja Dixit</name></author><author><name sortKey="Bergdall, Valerie" sort="Bergdall, Valerie" uniqKey="Bergdall V" first="Valerie" last="Bergdall">Valerie Bergdall</name></author><author><name sortKey="Roy, Sashwati" sort="Roy, Sashwati" uniqKey="Roy S" first="Sashwati" last="Roy">Sashwati Roy</name></author><author><name sortKey="Sen, Chandan K" sort="Sen, Chandan K" uniqKey="Sen C" first="Chandan K." last="Sen">Chandan K. Sen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25224310</idno><idno type="pmc">4380279</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4380279</idno><idno type="RBID">PMC:4380279</idno><idno type="doi">10.1111/wrr.12229</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">003964</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003964</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Modified Collagen Gel Dressing Promotes Angiogenesis in a Pre-Clinical Swine Model of Chronic Ischemic Wounds</title><author><name sortKey="Elgharably, Haytham" sort="Elgharably, Haytham" uniqKey="Elgharably H" first="Haytham" last="Elgharably">Haytham Elgharably</name></author><author><name sortKey="Ganesh, Kasturi" sort="Ganesh, Kasturi" uniqKey="Ganesh K" first="Kasturi" last="Ganesh">Kasturi Ganesh</name></author><author><name sortKey="Dickerson, Jennifer" sort="Dickerson, Jennifer" uniqKey="Dickerson J" first="Jennifer" last="Dickerson">Jennifer Dickerson</name></author><author><name sortKey="Khanna, Savita" sort="Khanna, Savita" uniqKey="Khanna S" first="Savita" last="Khanna">Savita Khanna</name></author><author><name sortKey="Abas, Motaz" sort="Abas, Motaz" uniqKey="Abas M" first="Motaz" last="Abas">Motaz Abas</name></author><author><name sortKey="Ghatak, Piya Das" sort="Ghatak, Piya Das" uniqKey="Ghatak P" first="Piya Das" last="Ghatak">Piya Das Ghatak</name></author><author><name sortKey="Dixit, Sriteja" sort="Dixit, Sriteja" uniqKey="Dixit S" first="Sriteja" last="Dixit">Sriteja Dixit</name></author><author><name sortKey="Bergdall, Valerie" sort="Bergdall, Valerie" uniqKey="Bergdall V" first="Valerie" last="Bergdall">Valerie Bergdall</name></author><author><name sortKey="Roy, Sashwati" sort="Roy, Sashwati" uniqKey="Roy S" first="Sashwati" last="Roy">Sashwati Roy</name></author><author><name sortKey="Sen, Chandan K" sort="Sen, Chandan K" uniqKey="Sen C" first="Chandan K." last="Sen">Chandan K. Sen</name></author></analytic><series><title level="j">Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society</title><idno type="ISSN">1067-1927</idno><idno type="eISSN">1524-475X</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">We recently performed proteomic characterization of a modified collagen gel (MCG) dressing and reported promising effects of the gel in healing full-thickness excisional wounds. In this work, we test the translational relevance of our aforesaid findings by testing the dressing in a swine model of chronic ischemic wounds recently reported by our laboratory. Full thickness excisional wounds were established in the center of bi- pedicle ischemic skin flaps on the backs of animals. Ischemia was verified by Laser Doppler imaging and MCG was applied to the test group of wounds. Seven days post- wounding, macrophage recruitment to the wound was significantly higher in MCG- treated ischemic wounds. <italic>In vitro</italic>, MCG up-regulated expression of Mrc-1 (a reparative M2 macrophage marker) and induced the expression of anti-inflammatory cytokine IL-10 and of β-FGF. An increased expression of CCR2, a M2 macrophage marker, was noted in the macrophages from MCG treated wounds. Furthermore, analyses of wound tissues 7 days post wounding showed up-regulation of TGF-β, VEGF, vWF, and collagen type I expression in MCG-treated ischemic wounds. At 21 days post-wounding, MCG-treated ischemic wounds displayed higher abundance of proliferating endothelial cells that formed mature vascular structures and increased blood flow to the wound. Fibroblast count was markedly higher in MCG-treated ischemic wound-edge tissue. In addition, MCG-treated wound-edge tissues displayed higher abundance of mature collagen with increased collagen type I:III deposition. Taken together, MCG helped mount a more robust inflammatory response which resolved in a timely manner, followed by an enhanced proliferative phase, angiogenic outcome and post-wound tissue remodeling. Findings of the current study warrant clinical testing of MCG in a setting of ischemic chronic wounds.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9310939</journal-id><journal-id journal-id-type="pubmed-jr-id">21119</journal-id><journal-id journal-id-type="nlm-ta">Wound Repair Regen</journal-id><journal-id journal-id-type="iso-abbrev">Wound Repair Regen</journal-id><journal-title-group><journal-title>Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society</journal-title></journal-title-group><issn pub-type="ppub">1067-1927</issn><issn pub-type="epub">1524-475X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">25224310</article-id><article-id pub-id-type="pmc">4380279</article-id><article-id pub-id-type="doi">10.1111/wrr.12229</article-id><article-id pub-id-type="manuscript">NIHMS628770</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A Modified Collagen Gel Dressing Promotes Angiogenesis in a Pre-Clinical Swine Model of Chronic Ischemic Wounds</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Elgharably</surname><given-names>Haytham</given-names></name><degrees>MD</degrees></contrib><contrib contrib-type="author"><name><surname>Ganesh</surname><given-names>Kasturi</given-names></name><degrees>MD</degrees></contrib><contrib contrib-type="author"><name><surname>Dickerson</surname><given-names>Jennifer</given-names></name><degrees>RVT</degrees></contrib><contrib contrib-type="author"><name><surname>Khanna</surname><given-names>Savita</given-names></name><degrees>PhD</degrees></contrib><contrib contrib-type="author"><name><surname>Abas</surname><given-names>Motaz</given-names></name><degrees>BS</degrees></contrib><contrib contrib-type="author"><name><surname>Ghatak</surname><given-names>Piya Das</given-names></name><degrees>MS</degrees></contrib><contrib contrib-type="author"><name><surname>Dixit</surname><given-names>Sriteja</given-names></name><degrees>MS</degrees></contrib><contrib contrib-type="author"><name><surname>Bergdall</surname><given-names>Valerie</given-names></name><degrees>DVM</degrees></contrib><contrib contrib-type="author"><name><surname>Roy</surname><given-names>Sashwati</given-names></name><degrees>PhD</degrees></contrib><contrib contrib-type="author"><name><surname>Sen</surname><given-names>Chandan K.</given-names></name><degrees>PhD</degrees></contrib><aff id="A1">Departments of Surgery, Davis Heart & Lung Research Institute, Center for Regenerative Medicine and Cell based Therapies and Comprehensive Wound Center, The Ohio State University Wexner Medical Center, Columbus, Ohio 43210</aff></contrib-group><author-notes><corresp id="FN1"><bold>Address correspondence to:</bold> Chandan K. Sen, PhD, 473 West 12th Avenue, 512 DHLRI, The Ohio State University Medical Center, Columbus, Ohio 43210, Tel. 614 247 7658; Fax 614 247 7818, <email>chandan.sen@osumc.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>16</day><month>3</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>08</day><month>1</month><year>2015</year></pub-date><pub-date pub-type="ppub"><season>Nov-Dec</season><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>08</day><month>1</month><year>2016</year></pub-date><volume>22</volume><issue>6</issue><fpage>720</fpage><lpage>729</lpage><pmc-comment>elocation-id from pubmed: 10.1111/wrr.12229</pmc-comment>
<abstract><p id="P1">We recently performed proteomic characterization of a modified collagen gel (MCG) dressing and reported promising effects of the gel in healing full-thickness excisional wounds. In this work, we test the translational relevance of our aforesaid findings by testing the dressing in a swine model of chronic ischemic wounds recently reported by our laboratory. Full thickness excisional wounds were established in the center of bi- pedicle ischemic skin flaps on the backs of animals. Ischemia was verified by Laser Doppler imaging and MCG was applied to the test group of wounds. Seven days post- wounding, macrophage recruitment to the wound was significantly higher in MCG- treated ischemic wounds. <italic>In vitro</italic>, MCG up-regulated expression of Mrc-1 (a reparative M2 macrophage marker) and induced the expression of anti-inflammatory cytokine IL-10 and of β-FGF. An increased expression of CCR2, a M2 macrophage marker, was noted in the macrophages from MCG treated wounds. Furthermore, analyses of wound tissues 7 days post wounding showed up-regulation of TGF-β, VEGF, vWF, and collagen type I expression in MCG-treated ischemic wounds. At 21 days post-wounding, MCG-treated ischemic wounds displayed higher abundance of proliferating endothelial cells that formed mature vascular structures and increased blood flow to the wound. Fibroblast count was markedly higher in MCG-treated ischemic wound-edge tissue. In addition, MCG-treated wound-edge tissues displayed higher abundance of mature collagen with increased collagen type I:III deposition. Taken together, MCG helped mount a more robust inflammatory response which resolved in a timely manner, followed by an enhanced proliferative phase, angiogenic outcome and post-wound tissue remodeling. Findings of the current study warrant clinical testing of MCG in a setting of ischemic chronic wounds.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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