Expression of lymphatic markers during avian and mouse cardiogenesis
Identifieur interne : 003532 ( Pmc/Corpus ); précédent : 003531; suivant : 003533Expression of lymphatic markers during avian and mouse cardiogenesis
Auteurs : Ganga Karunamuni ; Ke Yang ; Yong Qiu Doughman ; Jamie Wikenheiser ; David Bader ; Joey Barnett ; Anita Austin ; Patricia Parsons-Wingerter ; Michiko WatanabeSource :
- Anatomical record (Hoboken, N.J. : 2007) [ 1932-8486 ] ; 2010.
Abstract
The adult heart has been reported to have an extensive lymphatic system, yet the development of this important system during cardiogenesis is still largely unexplored. The nuclear-localized transcription factor Prox-1 identified a sheet of Prox-1-positive cells on the developing aorta and pulmonary trunk in avian and murine embryos just prior to septation of the four heart chambers. The cells coalesced into a branching lymphatic network that spread within the epicardium to cover the heart. These vessels eventually expressed the lymphatic markers LYVE-1, VEGFR-3, and podoplanin. Before the Prox-1-positive cells were detected in the mouse epicardium, LYVE-1, a homologue of the CD44 glycoprotein, was primarily expressed in individual epicardial cells. Similar staining patterns were observed for CD44 in avian embryos. The proximity of these LYVE-1/CD44-positive mesenchymal cells to Prox-1-positive vessels suggests that they may become incorporated into the lymphatics. Unexpectedly, we detected LYVE-1/PECAM/VEGFR-3-positive vessels within the embryonic and adult myocardium which remained Prox-1/podoplanin-negative. Lymphatic markers were surprisingly found in adult rat and embryonic mouse epicardial cell lines, with Prox-1 also exhibiting nuclear-localized expression in primary cultures of embryonic avian epicardial cells. Our data identified three types of cells in the embryonic heart expressing lymphatic markers: (1) Prox-1-positive cells from an extracardiac source that migrate within the serosa of the outflow tract into the epicardium of the developing heart, (2) individual LYVE-1-positive cells in the epicardium that may be incorporated into the Prox-1-positive lymphatic vasculature, and (3) LYVE-1-positive cells/vessels in the myocardium that do not become Prox-1-positive even in the adult heart.
Url:
DOI: 10.1002/ar.21043
PubMed: 19938109
PubMed Central: 3607319
Links to Exploration step
PMC:3607319Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression of lymphatic markers during avian and mouse cardiogenesis</title>
<author><name sortKey="Karunamuni, Ganga" sort="Karunamuni, Ganga" uniqKey="Karunamuni G" first="Ganga" last="Karunamuni">Ganga Karunamuni</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Anatomy, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Ke" sort="Yang, Ke" uniqKey="Yang K" first="Ke" last="Yang">Ke Yang</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Doughman, Yong Qiu" sort="Doughman, Yong Qiu" uniqKey="Doughman Y" first="Yong Qiu" last="Doughman">Yong Qiu Doughman</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wikenheiser, Jamie" sort="Wikenheiser, Jamie" uniqKey="Wikenheiser J" first="Jamie" last="Wikenheiser">Jamie Wikenheiser</name>
<affiliation><nlm:aff id="A3">Pathology and Laboratory Medicine, UCLA, Box 951732, Los Angeles, CA 90095</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bader, David" sort="Bader, David" uniqKey="Bader D" first="David" last="Bader">David Bader</name>
<affiliation><nlm:aff id="A4">Cell and Developmental Biology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Barnett, Joey" sort="Barnett, Joey" uniqKey="Barnett J" first="Joey" last="Barnett">Joey Barnett</name>
<affiliation><nlm:aff id="A5">Department of Pharmacology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Austin, Anita" sort="Austin, Anita" uniqKey="Austin A" first="Anita" last="Austin">Anita Austin</name>
<affiliation><nlm:aff id="A5">Department of Pharmacology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Parsons Wingerter, Patricia" sort="Parsons Wingerter, Patricia" uniqKey="Parsons Wingerter P" first="Patricia" last="Parsons-Wingerter">Patricia Parsons-Wingerter</name>
<affiliation><nlm:aff id="A6">Biological Fluid Physics, John Glenn NASA Research Center, MS 110-3 21000 Brookpark Rd., Cleveland, OH 44135</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Watanabe, Michiko" sort="Watanabe, Michiko" uniqKey="Watanabe M" first="Michiko" last="Watanabe">Michiko Watanabe</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19938109</idno>
<idno type="pmc">3607319</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607319</idno>
<idno type="RBID">PMC:3607319</idno>
<idno type="doi">10.1002/ar.21043</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">003532</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003532</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Expression of lymphatic markers during avian and mouse cardiogenesis</title>
<author><name sortKey="Karunamuni, Ganga" sort="Karunamuni, Ganga" uniqKey="Karunamuni G" first="Ganga" last="Karunamuni">Ganga Karunamuni</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Anatomy, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Ke" sort="Yang, Ke" uniqKey="Yang K" first="Ke" last="Yang">Ke Yang</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Doughman, Yong Qiu" sort="Doughman, Yong Qiu" uniqKey="Doughman Y" first="Yong Qiu" last="Doughman">Yong Qiu Doughman</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wikenheiser, Jamie" sort="Wikenheiser, Jamie" uniqKey="Wikenheiser J" first="Jamie" last="Wikenheiser">Jamie Wikenheiser</name>
<affiliation><nlm:aff id="A3">Pathology and Laboratory Medicine, UCLA, Box 951732, Los Angeles, CA 90095</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bader, David" sort="Bader, David" uniqKey="Bader D" first="David" last="Bader">David Bader</name>
<affiliation><nlm:aff id="A4">Cell and Developmental Biology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Barnett, Joey" sort="Barnett, Joey" uniqKey="Barnett J" first="Joey" last="Barnett">Joey Barnett</name>
<affiliation><nlm:aff id="A5">Department of Pharmacology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Austin, Anita" sort="Austin, Anita" uniqKey="Austin A" first="Anita" last="Austin">Anita Austin</name>
<affiliation><nlm:aff id="A5">Department of Pharmacology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Parsons Wingerter, Patricia" sort="Parsons Wingerter, Patricia" uniqKey="Parsons Wingerter P" first="Patricia" last="Parsons-Wingerter">Patricia Parsons-Wingerter</name>
<affiliation><nlm:aff id="A6">Biological Fluid Physics, John Glenn NASA Research Center, MS 110-3 21000 Brookpark Rd., Cleveland, OH 44135</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Watanabe, Michiko" sort="Watanabe, Michiko" uniqKey="Watanabe M" first="Michiko" last="Watanabe">Michiko Watanabe</name>
<affiliation><nlm:aff id="A1">Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Anatomical record (Hoboken, N.J. : 2007)</title>
<idno type="ISSN">1932-8486</idno>
<idno type="eISSN">1932-8494</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">The adult heart has been reported to have an extensive lymphatic system, yet the development of this important system during cardiogenesis is still largely unexplored. The nuclear-localized transcription factor Prox-1 identified a sheet of Prox-1-positive cells on the developing aorta and pulmonary trunk in avian and murine embryos just prior to septation of the four heart chambers. The cells coalesced into a branching lymphatic network that spread within the epicardium to cover the heart. These vessels eventually expressed the lymphatic markers LYVE-1, VEGFR-3, and podoplanin. Before the Prox-1-positive cells were detected in the mouse epicardium, LYVE-1, a homologue of the CD44 glycoprotein, was primarily expressed in individual epicardial cells. Similar staining patterns were observed for CD44 in avian embryos. The proximity of these LYVE-1/CD44-positive mesenchymal cells to Prox-1-positive vessels suggests that they may become incorporated into the lymphatics. Unexpectedly, we detected LYVE-1/PECAM/VEGFR-3-positive vessels within the embryonic and adult myocardium which remained Prox-1/podoplanin-negative. Lymphatic markers were surprisingly found in adult rat and embryonic mouse epicardial cell lines, with Prox-1 also exhibiting nuclear-localized expression in primary cultures of embryonic avian epicardial cells. Our data identified three types of cells in the embryonic heart expressing lymphatic markers: (1) Prox-1-positive cells from an extracardiac source that migrate within the serosa of the outflow tract into the epicardium of the developing heart, (2) individual LYVE-1-positive cells in the epicardium that may be incorporated into the Prox-1-positive lymphatic vasculature, and (3) LYVE-1-positive cells/vessels in the myocardium that do not become Prox-1-positive even in the adult heart.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101292775</journal-id>
<journal-id journal-id-type="pubmed-jr-id">33290</journal-id>
<journal-id journal-id-type="nlm-ta">Anat Rec (Hoboken)</journal-id>
<journal-id journal-id-type="iso-abbrev">Anat Rec (Hoboken)</journal-id>
<journal-title-group><journal-title>Anatomical record (Hoboken, N.J. : 2007)</journal-title>
</journal-title-group>
<issn pub-type="ppub">1932-8486</issn>
<issn pub-type="epub">1932-8494</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19938109</article-id>
<article-id pub-id-type="pmc">3607319</article-id>
<article-id pub-id-type="doi">10.1002/ar.21043</article-id>
<article-id pub-id-type="manuscript">NIHMS287022</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Expression of lymphatic markers during avian and mouse cardiogenesis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Karunamuni</surname>
<given-names>Ganga</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Ke</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Doughman</surname>
<given-names>Yong Qiu</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wikenheiser</surname>
<given-names>Jamie</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bader</surname>
<given-names>David</given-names>
</name>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Barnett</surname>
<given-names>Joey</given-names>
</name>
<xref ref-type="aff" rid="A5">5</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Austin</surname>
<given-names>Anita</given-names>
</name>
<xref ref-type="aff" rid="A5">5</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Parsons-Wingerter</surname>
<given-names>Patricia</given-names>
</name>
<xref ref-type="aff" rid="A6">6</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Watanabe</surname>
<given-names>Michiko</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Department of Pediatrics, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</aff>
<aff id="A2"><label>2</label>
Department of Anatomy, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, OH 44106</aff>
<aff id="A3"><label>3</label>
Pathology and Laboratory Medicine, UCLA, Box 951732, Los Angeles, CA 90095</aff>
<aff id="A4"><label>4</label>
Cell and Developmental Biology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</aff>
<aff id="A5"><label>5</label>
Department of Pharmacology, Vanderbilt University Medical Center, 21st Avenue South and Medical Center Drive, Nashville, TN 37232</aff>
<aff id="A6"><label>6</label>
Biological Fluid Physics, John Glenn NASA Research Center, MS 110-3 21000 Brookpark Rd., Cleveland, OH 44135</aff>
<author-notes><corresp id="FN1">Corresponding author: Dr. Michiko Watanabe, Department of Pediatrics, Cardiology 3<sup>rd</sup>
Floor, 2101 Adelbert Road, Cleveland, Ohio 44106. <email>mxw13@case.edu</email>
, phone: 216-844-7361, fax: 216-844-7642</corresp>
<corresp id="FN2">Correspondence concerning manuscript to: Ganga Karunamuni, 2101 Adelbert Road, RB&C Rm 8611, Cleveland, Ohio 44106. <email>ghk6@case.edu</email>
, phone: 216-844-7229, fax: 216-844-7642</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>15</day>
<month>2</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="ppub"><month>2</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>25</day>
<month>3</month>
<year>2013</year>
</pub-date>
<volume>293</volume>
<issue>2</issue>
<fpage>259</fpage>
<lpage>270</lpage>
<abstract><p id="P1">The adult heart has been reported to have an extensive lymphatic system, yet the development of this important system during cardiogenesis is still largely unexplored. The nuclear-localized transcription factor Prox-1 identified a sheet of Prox-1-positive cells on the developing aorta and pulmonary trunk in avian and murine embryos just prior to septation of the four heart chambers. The cells coalesced into a branching lymphatic network that spread within the epicardium to cover the heart. These vessels eventually expressed the lymphatic markers LYVE-1, VEGFR-3, and podoplanin. Before the Prox-1-positive cells were detected in the mouse epicardium, LYVE-1, a homologue of the CD44 glycoprotein, was primarily expressed in individual epicardial cells. Similar staining patterns were observed for CD44 in avian embryos. The proximity of these LYVE-1/CD44-positive mesenchymal cells to Prox-1-positive vessels suggests that they may become incorporated into the lymphatics. Unexpectedly, we detected LYVE-1/PECAM/VEGFR-3-positive vessels within the embryonic and adult myocardium which remained Prox-1/podoplanin-negative. Lymphatic markers were surprisingly found in adult rat and embryonic mouse epicardial cell lines, with Prox-1 also exhibiting nuclear-localized expression in primary cultures of embryonic avian epicardial cells. Our data identified three types of cells in the embryonic heart expressing lymphatic markers: (1) Prox-1-positive cells from an extracardiac source that migrate within the serosa of the outflow tract into the epicardium of the developing heart, (2) individual LYVE-1-positive cells in the epicardium that may be incorporated into the Prox-1-positive lymphatic vasculature, and (3) LYVE-1-positive cells/vessels in the myocardium that do not become Prox-1-positive even in the adult heart.</p>
</abstract>
<kwd-group><kwd>lymphatics</kwd>
<kwd>heart</kwd>
<kwd>Prox-1</kwd>
<kwd>LYVE-1</kwd>
<kwd>VEGFR-3</kwd>
<kwd>epicardium</kwd>
</kwd-group>
<funding-group><award-group><funding-source country="United States">National Institute of Environmental Health Sciences : NIEHS</funding-source>
<award-id>R21 ES013507-02 || ES</award-id>
</award-group>
<award-group><funding-source country="United States">National Institute of Environmental Health Sciences : NIEHS</funding-source>
<award-id>R21 ES013507-01 || ES</award-id>
</award-group>
<award-group><funding-source country="United States">National Heart, Lung, and Blood Institute : NHLBI</funding-source>
<award-id>R01 HL091171-02 || HL</award-id>
</award-group>
<award-group><funding-source country="United States">National Heart, Lung, and Blood Institute : NHLBI</funding-source>
<award-id>R01 HL091171-01A1 || HL</award-id>
</award-group>
</funding-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003532 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 003532 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Pmc |étape= Corpus |type= RBID |clé= PMC:3607319 |texte= Expression of lymphatic markers during avian and mouse cardiogenesis }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:19938109" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
This area was generated with Dilib version V0.6.31. |