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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Photoacoustic and photothermal cytometry for monitoring multiple blood rheology parameters <italic>in vivo</italic></title><author><name sortKey="Galanzha, Ekaterina I" sort="Galanzha, Ekaterina I" uniqKey="Galanzha E" first="Ekaterina I." last="Galanzha">Ekaterina I. Galanzha</name></author><author><name sortKey="Zharov, Vladimir P" sort="Zharov, Vladimir P" uniqKey="Zharov V" first="Vladimir P." last="Zharov">Vladimir P. Zharov</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21948731</idno><idno type="pmc">3734562</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734562</idno><idno type="RBID">PMC:3734562</idno><idno type="doi">10.1002/cyto.a.21133</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">003494</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003494</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Photoacoustic and photothermal cytometry for monitoring multiple blood rheology parameters <italic>in vivo</italic></title><author><name sortKey="Galanzha, Ekaterina I" sort="Galanzha, Ekaterina I" uniqKey="Galanzha E" first="Ekaterina I." last="Galanzha">Ekaterina I. Galanzha</name></author><author><name sortKey="Zharov, Vladimir P" sort="Zharov, Vladimir P" uniqKey="Zharov V" first="Vladimir P." last="Zharov">Vladimir P. Zharov</name></author></analytic><series><title level="j">Cytometry. Part A : the journal of the International Society for Analytical Cytology</title><idno type="ISSN">1552-4922</idno><idno type="eISSN">1552-4930</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Alterations of blood rheology (hemorheology) are important for the early diagnosis, prognosis, and prevention of many diseases, including myocardial infarction, stroke, sickle cell anemia, thromboembolism, trauma, inflammation, and malignancy. However, real-time <italic>in vivo</italic> monitoring of hemorheological status using multiple parameters over long periods of time has not been reported. Here we describe the capability of label-free photoacoustic (PA) and photothermal (PT) flow cytometry in detection and imaging modes for dynamic monitoring of rheological parameters in circulating blood. We show that this integrated platform can simultaneously measure the main rheological parameters and may improve their diagnostic value. Using phenomenological approaches, we analyze correlations of PT and PA signal characteristics in the dynamic modes with red blood cell (RBC) aggregation, deformability, shape (e.g., as in sickle cells), intracellular hemoglobin distribution, individual cell velocity, flux of RBCs, and likely shear rate. Proof of concept is demonstrated in <italic>ex vivo</italic> and <italic>in vivo</italic> tests, including high-speed PT imaging of RBC shape in pathological conditions and identification of sickle cells in a mouse model of human sickle cell disease. These studies revealed the potential of this new platform integrating PT, PA, and conventional optical techniques for translation to use in humans using safe, portable, laser-based medical devices for point-of-care screening of disease progression and therapy efficiency.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101235694</journal-id><journal-id journal-id-type="pubmed-jr-id">32205</journal-id><journal-id journal-id-type="nlm-ta">Cytometry A</journal-id><journal-id journal-id-type="iso-abbrev">Cytometry A</journal-id><journal-title-group><journal-title>Cytometry. Part A : the journal of the International Society for Analytical Cytology</journal-title></journal-title-group><issn pub-type="ppub">1552-4922</issn><issn pub-type="epub">1552-4930</issn></journal-meta><article-meta><article-id pub-id-type="pmid">21948731</article-id><article-id pub-id-type="pmc">3734562</article-id><article-id pub-id-type="doi">10.1002/cyto.a.21133</article-id><article-id pub-id-type="manuscript">NIHMS336717</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Photoacoustic and photothermal cytometry for monitoring multiple blood rheology parameters <italic>in vivo</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Galanzha</surname><given-names>Ekaterina I.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zharov</surname><given-names>Vladimir P.</given-names></name><xref rid="FN1" ref-type="author-notes">*</xref></contrib><aff id="A1">Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205</aff></contrib-group><author-notes><corresp id="FN1"><label>*</label>Correspondence to V.P. Zharov, Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, <email>zharovvladimirp@uams.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>17</day><month>4</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>30</day><month>8</month><year>2011</year></pub-date><pub-date pub-type="ppub"><month>10</month><year>2011</year></pub-date><pub-date pub-type="pmc-release"><day>06</day><month>8</month><year>2013</year></pub-date><volume>79</volume><issue>10</issue><fpage>746</fpage><lpage>757</lpage><abstract><p id="P1">Alterations of blood rheology (hemorheology) are important for the early diagnosis, prognosis, and prevention of many diseases, including myocardial infarction, stroke, sickle cell anemia, thromboembolism, trauma, inflammation, and malignancy. However, real-time <italic>in vivo</italic> monitoring of hemorheological status using multiple parameters over long periods of time has not been reported. Here we describe the capability of label-free photoacoustic (PA) and photothermal (PT) flow cytometry in detection and imaging modes for dynamic monitoring of rheological parameters in circulating blood. We show that this integrated platform can simultaneously measure the main rheological parameters and may improve their diagnostic value. Using phenomenological approaches, we analyze correlations of PT and PA signal characteristics in the dynamic modes with red blood cell (RBC) aggregation, deformability, shape (e.g., as in sickle cells), intracellular hemoglobin distribution, individual cell velocity, flux of RBCs, and likely shear rate. Proof of concept is demonstrated in <italic>ex vivo</italic> and <italic>in vivo</italic> tests, including high-speed PT imaging of RBC shape in pathological conditions and identification of sickle cells in a mouse model of human sickle cell disease. These studies revealed the potential of this new platform integrating PT, PA, and conventional optical techniques for translation to use in humans using safe, portable, laser-based medical devices for point-of-care screening of disease progression and therapy efficiency.</p></abstract><funding-group><award-group><funding-source country="United States">National Cancer Institute : NCI</funding-source><award-id>R21 CA139373-02 || CA</award-id></award-group><award-group><funding-source country="United States">National Institute of Biomedical Imaging and Bioengineering : NIBIB</funding-source><award-id>R01 EB009230-03 || EB</award-id></award-group><award-group><funding-source country="United States">National Institute of Biomedical Imaging and Bioengineering : NIBIB</funding-source><award-id>R01 EB000873-03 || EB</award-id></award-group><award-group><funding-source country="United States">National Cancer Institute : NCI</funding-source><award-id>R01 CA131164-03 || CA</award-id></award-group></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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