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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Engendering Allograft Ignorance in a Mouse Model of Allogeneic Skin Transplantation to the Distal Hind Limb</title><author><name sortKey="Agarwal, Shailesh" sort="Agarwal, Shailesh" uniqKey="Agarwal S" first="Shailesh" last="Agarwal">Shailesh Agarwal</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Loder, Shawn" sort="Loder, Shawn" uniqKey="Loder S" first="Shawn" last="Loder">Shawn Loder</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Wood, Sherri" sort="Wood, Sherri" uniqKey="Wood S" first="Sherri" last="Wood">Sherri Wood</name><affiliation><nlm:aff id="A2">Department of Surgery, Section of Transplant Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Cederna, Paul S" sort="Cederna, Paul S" uniqKey="Cederna P" first="Paul S." last="Cederna">Paul S. Cederna</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Bishop, D Keith" sort="Bishop, D Keith" uniqKey="Bishop D" first="D. Keith" last="Bishop">D. Keith Bishop</name><affiliation><nlm:aff id="A2">Department of Surgery, Section of Transplant Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Wang, Stewart C" sort="Wang, Stewart C" uniqKey="Wang S" first="Stewart C." last="Wang">Stewart C. Wang</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Department of Surgery, Division of Burn Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Levi, Benjamin" sort="Levi, Benjamin" uniqKey="Levi B" first="Benjamin" last="Levi">Benjamin Levi</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Department of Surgery, Division of Burn Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24509194</idno><idno type="pmc">4573553</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573553</idno><idno type="RBID">PMC:4573553</idno><idno type="doi">10.1097/SLA.0000000000000572</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">003343</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003343</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Engendering Allograft Ignorance in a Mouse Model of Allogeneic Skin Transplantation to the Distal Hind Limb</title><author><name sortKey="Agarwal, Shailesh" sort="Agarwal, Shailesh" uniqKey="Agarwal S" first="Shailesh" last="Agarwal">Shailesh Agarwal</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Loder, Shawn" sort="Loder, Shawn" uniqKey="Loder S" first="Shawn" last="Loder">Shawn Loder</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Wood, Sherri" sort="Wood, Sherri" uniqKey="Wood S" first="Sherri" last="Wood">Sherri Wood</name><affiliation><nlm:aff id="A2">Department of Surgery, Section of Transplant Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Cederna, Paul S" sort="Cederna, Paul S" uniqKey="Cederna P" first="Paul S." last="Cederna">Paul S. Cederna</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Bishop, D Keith" sort="Bishop, D Keith" uniqKey="Bishop D" first="D. Keith" last="Bishop">D. Keith Bishop</name><affiliation><nlm:aff id="A2">Department of Surgery, Section of Transplant Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Wang, Stewart C" sort="Wang, Stewart C" uniqKey="Wang S" first="Stewart C." last="Wang">Stewart C. Wang</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Department of Surgery, Division of Burn Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author><author><name sortKey="Levi, Benjamin" sort="Levi, Benjamin" uniqKey="Levi B" first="Benjamin" last="Levi">Benjamin Levi</name><affiliation><nlm:aff id="A1">Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Department of Surgery, Division of Burn Surgery, University of Michigan, Ann Arbor, MI</nlm:aff></affiliation></author></analytic><series><title level="j">Annals of surgery</title><idno type="ISSN">0003-4932</idno><idno type="eISSN">1528-1140</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Objective</title><p id="P1">The aim of this study was to demonstrate lymphatic isolation in a model of hind limb lymph node (LN) excision, consisting of ipsilateral popliteal and inguinal LN excision and to evaluate the immunologic response to allogeneic skin transplanted onto this region of lymphatic isolation.</p></sec><sec id="S2"><title>Methods</title><p id="P2">To study lymphatic flow, C57BL/6 mice underwent lymphadenectomy (n = 5), sham lymphadenectomy (n = 5), or no intervention (n = 5), followed by methylene blue injection. Mice were dissected to determine whether methylene blue traveled to the iliac LN. To study host response to skin transplantation, C57BL/6 mice underwent allogeneic skin transplantation with LN excision (n = 6), allogeneic skin transplantation alone (n = 6), or syngeneic skin transplantation (n = 4). Skin grafts were placed distal to the popliteal fossa and mice were euthanized at day 10. Grafts were stained for endothelial cell and proliferation markers (CD31 and Ki67, respectively). Secondary lymphoid tissues (spleen, ipsilateral axillary LN, and contralateral inguinal LN) were removed and rechallenged with BALB/c alloantigen in vitro with subsequent assay of interferon-<italic>γ</italic> and interleukin 4 cell expression using ELISPOT technique.</p></sec><sec id="S3"><title>Results</title><p id="P3">Mice that underwent LN excision had no evidence of methylene blue in the iliac nodes; mice without surgical intervention or with sham LN excision consistently had methylene blue visible in the ipsilateral iliac nodes. Mice treated with allogeneic skin transplantation and LN excision had lower expression of interferon-<italic>γ</italic> and interleukin 4 in the secondary lymphoid tissues.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">Lymph node excision completely interrupts lymphatic flow of the hind limb. This model of lymphatic isolation impairs the ability of the transplant recipient to acutely mount a Th1 or Th2 response to allogeneic skin transplants.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0372354</journal-id><journal-id journal-id-type="pubmed-jr-id">646</journal-id><journal-id journal-id-type="nlm-ta">Ann Surg</journal-id><journal-id journal-id-type="iso-abbrev">Ann. Surg.</journal-id><journal-title-group><journal-title>Annals of surgery</journal-title></journal-title-group><issn pub-type="ppub">0003-4932</issn><issn pub-type="epub">1528-1140</issn></journal-meta><article-meta><article-id pub-id-type="pmid">24509194</article-id><article-id pub-id-type="pmc">4573553</article-id><article-id pub-id-type="doi">10.1097/SLA.0000000000000572</article-id><article-id pub-id-type="manuscript">NIHMS661564</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Engendering Allograft Ignorance in a Mouse Model of Allogeneic Skin Transplantation to the Distal Hind Limb</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Agarwal</surname><given-names>Shailesh</given-names></name><degrees>MD</degrees><xref ref-type="aff" rid="A1">*</xref></contrib><contrib contrib-type="author"><name><surname>Loder</surname><given-names>Shawn</given-names></name><degrees>BA</degrees><xref ref-type="aff" rid="A1">*</xref></contrib><contrib contrib-type="author"><name><surname>Wood</surname><given-names>Sherri</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="A2">†</xref></contrib><contrib contrib-type="author"><name><surname>Cederna</surname><given-names>Paul S.</given-names></name><degrees>MD</degrees><xref ref-type="aff" rid="A1">*</xref></contrib><contrib contrib-type="author"><name><surname>Bishop</surname><given-names>D. Keith</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="A2">†</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Stewart C.</given-names></name><degrees>MD, PhD</degrees><xref ref-type="aff" rid="A1">*</xref><xref ref-type="aff" rid="A3">‡</xref></contrib><contrib contrib-type="author"><name><surname>Levi</surname><given-names>Benjamin</given-names></name><degrees>MD</degrees><xref ref-type="aff" rid="A1">*</xref><xref ref-type="aff" rid="A3">‡</xref></contrib></contrib-group><aff id="A1"><label>*</label>Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI</aff><aff id="A2"><label>†</label>Department of Surgery, Section of Transplant Surgery, University of Michigan, Ann Arbor, MI</aff><aff id="A3"><label>‡</label>Department of Surgery, Division of Burn Surgery, University of Michigan, Ann Arbor, MI</aff><author-notes><corresp id="FN1">Reprints: Benjamin Levi, MD, Department of Surgery, Section of Plastic Surgery, University of Michigan, 1500 East Medical Center Dr, Ann Arbor, MI 48109. <email>blevi@umich.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>7</day><month>2</month><year>2015</year></pub-date><pub-date pub-type="ppub"><month>3</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>17</day><month>9</month><year>2015</year></pub-date><volume>261</volume><issue>3</issue><fpage>611</fpage><lpage>618</lpage><pmc-comment>elocation-id from pubmed: 10.1097/SLA.0000000000000572</pmc-comment>
<abstract><sec id="S1"><title>Objective</title><p id="P1">The aim of this study was to demonstrate lymphatic isolation in a model of hind limb lymph node (LN) excision, consisting of ipsilateral popliteal and inguinal LN excision and to evaluate the immunologic response to allogeneic skin transplanted onto this region of lymphatic isolation.</p></sec><sec id="S2"><title>Methods</title><p id="P2">To study lymphatic flow, C57BL/6 mice underwent lymphadenectomy (n = 5), sham lymphadenectomy (n = 5), or no intervention (n = 5), followed by methylene blue injection. Mice were dissected to determine whether methylene blue traveled to the iliac LN. To study host response to skin transplantation, C57BL/6 mice underwent allogeneic skin transplantation with LN excision (n = 6), allogeneic skin transplantation alone (n = 6), or syngeneic skin transplantation (n = 4). Skin grafts were placed distal to the popliteal fossa and mice were euthanized at day 10. Grafts were stained for endothelial cell and proliferation markers (CD31 and Ki67, respectively). Secondary lymphoid tissues (spleen, ipsilateral axillary LN, and contralateral inguinal LN) were removed and rechallenged with BALB/c alloantigen in vitro with subsequent assay of interferon-<italic>γ</italic> and interleukin 4 cell expression using ELISPOT technique.</p></sec><sec id="S3"><title>Results</title><p id="P3">Mice that underwent LN excision had no evidence of methylene blue in the iliac nodes; mice without surgical intervention or with sham LN excision consistently had methylene blue visible in the ipsilateral iliac nodes. Mice treated with allogeneic skin transplantation and LN excision had lower expression of interferon-<italic>γ</italic> and interleukin 4 in the secondary lymphoid tissues.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">Lymph node excision completely interrupts lymphatic flow of the hind limb. This model of lymphatic isolation impairs the ability of the transplant recipient to acutely mount a Th1 or Th2 response to allogeneic skin transplants.</p></sec></abstract><kwd-group><kwd>antigen presentation</kwd><kwd>ELISPOT</kwd><kwd>lymph node</kwd><kwd>lymphadenectomy</kwd><kwd>lymphedema</kwd><kwd>methylene blue</kwd><kwd>sentinel node</kwd><kwd>skin transplant</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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