An Immunological Fingerprint Differentiates Muscular Lymphatics from Arteries and Veins
Identifieur interne : 003034 ( Pmc/Corpus ); précédent : 003033; suivant : 003035An Immunological Fingerprint Differentiates Muscular Lymphatics from Arteries and Veins
Auteurs : Eric A. Bridenbaugh ; Wei Wang ; Maya Srimushnam ; Walter E. Cromer ; Scott D. Zawieja ; Susan E. Schmidt ; Daniel C. Jupiter ; Hung-Chung Huang ; Vincent Van Buren ; David C. ZawiejaSource :
- Lymphatic Research and Biology [ 1539-6851 ] ; 2013.
Abstract
The principal function of the lymphatic system is to transport lymph from the interstitium to the nodes and then from the nodes to the blood. In doing so lymphatics play important roles in fluid homeostasis, macromolecular/antigen transport and immune cell trafficking. To better understand the genes that contribute to their unique physiology, we compared the transcriptional profile of muscular lymphatics (prenodal mesenteric microlymphatics and large, postnodal thoracic duct) to axillary and mesenteric arteries and veins isolated from rats. Clustering of the differentially expressed genes demonstrated that the lymph versus blood vessel differences were more profound than between blood vessels, particularly the microvessels. Gene ontology functional category analysis indicated that microlymphatics were enriched in antigen processing/presentation, IgE receptor signaling, catabolic processes, translation and ribosome; while they were diminished in oxygen transport, regulation of cell proliferation, glycolysis and inhibition of adenylate cyclase activity by G-proteins. We evaluated the differentially expressed microarray genes/products by qPCR and/or immunofluorescence. Immunofluorescence documented that multiple MHC class II antigen presentation proteins were highly expressed by an antigen-presenting cell (APC) type found resident within the lymphatic wall. These APCs also expressed CD86, a co-stimulatory protein necessary for T-cell activation. We evaluated the distribution and phenotype of APCs within the pre and postnodal lymphatic network. This study documents a novel population of APCs resident within the walls of muscular, prenodal lymphatics that indicates novel roles in antigen sampling and immune responses. In conclusion, these prenodal lymphatics exhibit a unique profile that distinguishes them from blood vessels and highlights the role of the lymphatic system as an immunovascular system linking the parenchymal interstitium, lymph nodes and the blood.
Url:
DOI: 10.1089/lrb.2013.0023
PubMed: 24044756
PubMed Central: 3780313
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PMC:3780313Le document en format XML
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<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
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<author><name sortKey="Jupiter, Daniel C" sort="Jupiter, Daniel C" uniqKey="Jupiter D" first="Daniel C." last="Jupiter">Daniel C. Jupiter</name>
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<author><name sortKey="Huang, Hung Chung" sort="Huang, Hung Chung" uniqKey="Huang H" first="Hung-Chung" last="Huang">Hung-Chung Huang</name>
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<series><title level="j">Lymphatic Research and Biology</title>
<idno type="ISSN">1539-6851</idno>
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<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>The principal function of the lymphatic system is to transport lymph from the interstitium to the nodes and then from the nodes to the blood. In doing so lymphatics play important roles in fluid homeostasis, macromolecular/antigen transport and immune cell trafficking. To better understand the genes that contribute to their unique physiology, we compared the transcriptional profile of muscular lymphatics (prenodal mesenteric microlymphatics and large, postnodal thoracic duct) to axillary and mesenteric arteries and veins isolated from rats. Clustering of the differentially expressed genes demonstrated that the lymph versus blood vessel differences were more profound than between blood vessels, particularly the microvessels. Gene ontology functional category analysis indicated that microlymphatics were enriched in antigen processing/presentation, IgE receptor signaling, catabolic processes, translation and ribosome; while they were diminished in oxygen transport, regulation of cell proliferation, glycolysis and inhibition of adenylate cyclase activity by G-proteins. We evaluated the differentially expressed microarray genes/products by qPCR and/or immunofluorescence. Immunofluorescence documented that multiple MHC class II antigen presentation proteins were highly expressed by an antigen-presenting cell (APC) type found resident within the lymphatic wall. These APCs also expressed CD86, a co-stimulatory protein necessary for T-cell activation. We evaluated the distribution and phenotype of APCs within the pre and postnodal lymphatic network. This study documents a novel population of APCs resident within the walls of muscular, prenodal lymphatics that indicates novel roles in antigen sampling and immune responses. In conclusion, these prenodal lymphatics exhibit a unique profile that distinguishes them from blood vessels and highlights the role of the lymphatic system as an immunovascular system linking the parenchymal interstitium, lymph nodes and the blood.</p>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Lymphat Res Biol</journal-id>
<journal-id journal-id-type="iso-abbrev">Lymphat Res Biol</journal-id>
<journal-id journal-id-type="publisher-id">lrb</journal-id>
<journal-title-group><journal-title>Lymphatic Research and Biology</journal-title>
</journal-title-group>
<issn pub-type="ppub">1539-6851</issn>
<issn pub-type="epub">1557-8585</issn>
<publisher><publisher-name>Mary Ann Liebert, Inc.</publisher-name>
<publisher-loc>140 Huguenot Street, 3rd FloorNew Rochelle, NY 10801USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24044756</article-id>
<article-id pub-id-type="pmc">3780313</article-id>
<article-id pub-id-type="publisher-id">10.1089/lrb.2013.0023</article-id>
<article-id pub-id-type="doi">10.1089/lrb.2013.0023</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>An Immunological Fingerprint Differentiates Muscular Lymphatics from Arteries and Veins</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Bridenbaugh</surname>
<given-names>Eric A.</given-names>
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<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Wei</given-names>
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<contrib contrib-type="author"><name><surname>Srimushnam</surname>
<given-names>Maya</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Cromer</surname>
<given-names>Walter E.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Zawieja</surname>
<given-names>Scott D.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Schmidt</surname>
<given-names>Susan E.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Jupiter</surname>
<given-names>Daniel C.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Huang</surname>
<given-names>Hung-Chung</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Van Buren</surname>
<given-names>Vincent</given-names>
</name>
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<contrib contrib-type="author" corresp="yes"><name><surname>Zawieja</surname>
<given-names>David C.</given-names>
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<aff id="aff1">Department of Medical Physiology, Division of Lymphatic Biology,<institution>Texas A&M Health Science Center College of Medicine</institution>
, Temple, Texas.</aff>
</contrib-group>
<author-notes><corresp>Address correspondence to: <italic>David C. Zawieja, Department of Medical Physiology, Texas A&M Health Science Center, College of Medicine, 702 S.W. H.K. Dodgen Loop, Temple, TX 76504. E-mail:</italic>
<email xlink:href="mailto:dcz@tamu.edu">dcz@tamu.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>9</month>
<year>2013</year>
<pmc-comment>string-date: September 2013</pmc-comment>
</pub-date>
<volume>11</volume>
<issue>3</issue>
<fpage>155</fpage>
<lpage>171</lpage>
<permissions><copyright-statement>Copyright 2013, Mary Ann Liebert, Inc.</copyright-statement>
<copyright-year>2013</copyright-year>
</permissions>
<self-uri xlink:type="simple" xlink:href="lrb.2013.0023.pdf"></self-uri>
<abstract><title>Abstract</title>
<p>The principal function of the lymphatic system is to transport lymph from the interstitium to the nodes and then from the nodes to the blood. In doing so lymphatics play important roles in fluid homeostasis, macromolecular/antigen transport and immune cell trafficking. To better understand the genes that contribute to their unique physiology, we compared the transcriptional profile of muscular lymphatics (prenodal mesenteric microlymphatics and large, postnodal thoracic duct) to axillary and mesenteric arteries and veins isolated from rats. Clustering of the differentially expressed genes demonstrated that the lymph versus blood vessel differences were more profound than between blood vessels, particularly the microvessels. Gene ontology functional category analysis indicated that microlymphatics were enriched in antigen processing/presentation, IgE receptor signaling, catabolic processes, translation and ribosome; while they were diminished in oxygen transport, regulation of cell proliferation, glycolysis and inhibition of adenylate cyclase activity by G-proteins. We evaluated the differentially expressed microarray genes/products by qPCR and/or immunofluorescence. Immunofluorescence documented that multiple MHC class II antigen presentation proteins were highly expressed by an antigen-presenting cell (APC) type found resident within the lymphatic wall. These APCs also expressed CD86, a co-stimulatory protein necessary for T-cell activation. We evaluated the distribution and phenotype of APCs within the pre and postnodal lymphatic network. This study documents a novel population of APCs resident within the walls of muscular, prenodal lymphatics that indicates novel roles in antigen sampling and immune responses. In conclusion, these prenodal lymphatics exhibit a unique profile that distinguishes them from blood vessels and highlights the role of the lymphatic system as an immunovascular system linking the parenchymal interstitium, lymph nodes and the blood.</p>
</abstract>
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