The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish*
Identifieur interne : 002E91 ( Pmc/Corpus ); précédent : 002E90; suivant : 002E92The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish*
Auteurs : Heng Huang ; Ting Jin ; Lin Wang ; Fulong Wang ; Rui Zhang ; Yi Pan ; Zhenzhen Wang ; Yan ChenSource :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2012.
Abstract
Url:
DOI: 10.1074/jbc.M112.418202
PubMed: 23184941
PubMed Central: 3554906
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish<xref ref-type="fn" rid="FN1">*</xref>
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<author><name sortKey="Huang, Heng" sort="Huang, Heng" uniqKey="Huang H" first="Heng" last="Huang">Heng Huang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jin, Ting" sort="Jin, Ting" uniqKey="Jin T" first="Ting" last="Jin">Ting Jin</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
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<author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Fulong" sort="Wang, Fulong" uniqKey="Wang F" first="Fulong" last="Wang">Fulong Wang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Rui" sort="Zhang, Rui" uniqKey="Zhang R" first="Rui" last="Zhang">Rui Zhang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pan, Yi" sort="Pan, Yi" uniqKey="Pan Y" first="Yi" last="Pan">Yi Pan</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Zhenzhen" sort="Wang, Zhenzhen" uniqKey="Wang Z" first="Zhenzhen" last="Wang">Zhenzhen Wang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Yan" sort="Chen, Yan" uniqKey="Chen Y" first="Yan" last="Chen">Yan Chen</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
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<date when="2012">2012</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish<xref ref-type="fn" rid="FN1">*</xref>
<xref ref-type="fn" rid="FN2"><sup><inline-graphic xlink:href="sbox.jpg"></inline-graphic>
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<author><name sortKey="Huang, Heng" sort="Huang, Heng" uniqKey="Huang H" first="Heng" last="Huang">Heng Huang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jin, Ting" sort="Jin, Ting" uniqKey="Jin T" first="Ting" last="Jin">Ting Jin</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Fulong" sort="Wang, Fulong" uniqKey="Wang F" first="Fulong" last="Wang">Fulong Wang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Rui" sort="Zhang, Rui" uniqKey="Zhang R" first="Rui" last="Zhang">Rui Zhang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pan, Yi" sort="Pan, Yi" uniqKey="Pan Y" first="Yi" last="Pan">Yi Pan</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Zhenzhen" sort="Wang, Zhenzhen" uniqKey="Wang Z" first="Zhenzhen" last="Wang">Zhenzhen Wang</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Yan" sort="Chen, Yan" uniqKey="Chen Y" first="Yan" last="Chen">Yan Chen</name>
<affiliation><nlm:aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of Biological Chemistry</title>
<idno type="ISSN">0021-9258</idno>
<idno type="eISSN">1083-351X</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
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<front><div type="abstract" xml:lang="en"><p><bold>Background:</bold>
RasGRP1 is a protein involved in Ras activation, but its physiological function is largely unknown.</p>
<p><bold>Results:</bold>
Knockdown of RasGRP1 impairs development of the lymphatic system in zebrafish embryos, shown as defective thoracic duct formation, pericardial and truck edema, and altered sprouts of lymphatic vessels.</p>
<p><bold>Conclusion:</bold>
RasGRP1 is involved in lymphangiogenesis in zebrafish.</p>
<p><bold>Significance:</bold>
RasGRP1 has a physiological function in early development.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Biol Chem</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Biol. Chem</journal-id>
<journal-id journal-id-type="hwp">jbc</journal-id>
<journal-id journal-id-type="pmc">jbc</journal-id>
<journal-id journal-id-type="publisher-id">JBC</journal-id>
<journal-title-group><journal-title>The Journal of Biological Chemistry</journal-title>
</journal-title-group>
<issn pub-type="ppub">0021-9258</issn>
<issn pub-type="epub">1083-351X</issn>
<publisher><publisher-name>American Society for Biochemistry and Molecular Biology</publisher-name>
<publisher-loc>9650 Rockville Pike, Bethesda, MD 20814, U.S.A.</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23184941</article-id>
<article-id pub-id-type="pmc">3554906</article-id>
<article-id pub-id-type="publisher-id">M112.418202</article-id>
<article-id pub-id-type="doi">10.1074/jbc.M112.418202</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Developmental Biology</subject>
</subj-group>
</article-categories>
<title-group><article-title>The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish<xref ref-type="fn" rid="FN1">*</xref>
<xref ref-type="fn" rid="FN2"><sup><inline-graphic xlink:href="sbox.jpg"></inline-graphic>
</sup>
</xref>
</article-title>
<alt-title alt-title-type="short">RasGRP1 in Lymphatic Development</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Huang</surname>
<given-names>Heng</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jin</surname>
<given-names>Ting</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Lin</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Fulong</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Rui</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pan</surname>
<given-names>Yi</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Zhenzhen</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Yan</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
<xref ref-type="corresp" rid="cor1"><sup>1</sup>
</xref>
</contrib>
<aff id="aff1">From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>1</label>
To whom correspondence should be addressed: <addr-line>Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Taiyuan Rd., Shanghai 200031, China.</addr-line>
Tel.: <phone>86-21-54920916</phone>
; Fax: <fax>86-21-54920291</fax>
; E-mail: <email>ychen3@sibs.ac.cn</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><day>25</day>
<month>1</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>26</day>
<month>11</month>
<year>2012</year>
</pub-date>
<volume>288</volume>
<issue>4</issue>
<fpage>2355</fpage>
<lpage>2364</lpage>
<history><date date-type="received"><day>10</day>
<month>9</month>
<year>2012</year>
</date>
<date date-type="rev-recd"><day>21</day>
<month>11</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.</copyright-statement>
<copyright-year>2013</copyright-year>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zbc00413002355.pdf"></self-uri>
<abstract abstract-type="teaser"><p><bold>Background:</bold>
RasGRP1 is a protein involved in Ras activation, but its physiological function is largely unknown.</p>
<p><bold>Results:</bold>
Knockdown of RasGRP1 impairs development of the lymphatic system in zebrafish embryos, shown as defective thoracic duct formation, pericardial and truck edema, and altered sprouts of lymphatic vessels.</p>
<p><bold>Conclusion:</bold>
RasGRP1 is involved in lymphangiogenesis in zebrafish.</p>
<p><bold>Significance:</bold>
RasGRP1 has a physiological function in early development.</p>
</abstract>
<abstract><p>The molecular basis of the lymphatic development remains largely unknown. Using zebrafish as a model, we discovered a novel role for the Ras guanine-releasing protein 1 (RasGRP1), a protein involved in Ras activation in lymphangiogenesis. Secondary lymphatic sprouts from the posterior cardinal vein give rise to thoracic duct which is the first lymphatic vessel in zebrafish. Knockdown of <italic>rasgrp1</italic>
by injecting morpholino in zebrafish embryos impaired formation of thoracic duct accompanied by pericardial and truck edema, whereas blood vessel development of the embryos was largely unaffected. In <italic>rasgrp1</italic>
-knockdown embryos, the number of sprouts producing the string of parachordal lymphangioblast cells was reduced. Meanwhile the total number of the secondary sprouts was not changed. As a result, the number of venous intersegmental vessels was increased, whereas the number of lymphatic vessel was reduced at a later stage. The lymphatic developmental defects caused by <italic>rasgrp1</italic>
knockdown could be rescued by ectopic expression of a constitutively active HRas. Further analysis revealed that RasGRP1 knockdown could synergize with <italic>flt4/vegfr3</italic>
knockdown to induce defects in lymphangiogenesis. Taken together, this finding demonstrates a critical role for RasGRP1 in lymphatic development in zebrafish.</p>
</abstract>
<kwd-group><kwd>Development</kwd>
<kwd>Lymphangiogenesis</kwd>
<kwd>Ras</kwd>
<kwd>Vascular Biology</kwd>
<kwd>Vascular Endothelial Growth Factor (VEGF)</kwd>
<kwd>Zebrafish</kwd>
<kwd>PAQR10</kwd>
<kwd>RasGRP1</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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