Human microvascular lymphatic and blood endothelial cells produce fibrillin: deposition patterns and quantitative analysis
Identifieur interne : 002E84 ( Pmc/Corpus ); précédent : 002E83; suivant : 002E85Human microvascular lymphatic and blood endothelial cells produce fibrillin: deposition patterns and quantitative analysis
Auteurs : Antonella Rossi ; Erica Gabbrielli ; Marilisa Villano ; Mario Messina ; Francesco Ferrara ; Elisabetta WeberSource :
- Journal of Anatomy [ 0021-8782 ] ; 2010.
Abstract
Fibrillin microfibrils constitute a scaffold for elastin deposition in the wall of arteries and form the anchoring filaments that connect the lymphatic endothelium to surrounding elastic fibers. We previously reported that fibrillin is deposited in a honeycomb pattern in bovine arterial endothelial cells, which also deposit microfibril-associated glycoprotein (MAGP)-1, whereas thoracic duct endothelial cells form an irregular web. The present immunohistochemical study was designed to verify whether lymphatic and blood human dermal microvascular endothelial cells (HDMECs) isolated from human foreskin by the sequential use of a pan-endothelial marker, CD31, and the lymphatic specific marker, D2-40, deposit fibrillin and MAGP-1. In both cell types, fibrillin and MAGP-1 co-localized and were deposited with different patterns of increasing complexity co-existing in the same culture. Fibrillin microfibrils formed a wide-mesh honeycomb leaving fibrillin-free spaces that were gradually filled. This modality of fibrillin deposition, similar to that of bovine large artery endothelial cells, was basically the same in blood and lymphatic HDMECs. In some lymphatic HDMECs, fibrillin was initially deposited as uniformly scattered short fibrillin strands probably as a result of anchoring filaments carried over from the vessels of origin. Our findings show that blood and lymphatic endothelial cells participate in fibrillin deposition in human skin.
Url:
DOI: 10.1111/j.1469-7580.2010.01306.x
PubMed: 21039476
PubMed Central: 3039183
Links to Exploration step
PMC:3039183Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Human microvascular lymphatic and blood endothelial cells produce fibrillin: deposition patterns and quantitative analysis</title>
<author><name sortKey="Rossi, Antonella" sort="Rossi, Antonella" uniqKey="Rossi A" first="Antonella" last="Rossi">Antonella Rossi</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gabbrielli, Erica" sort="Gabbrielli, Erica" uniqKey="Gabbrielli E" first="Erica" last="Gabbrielli">Erica Gabbrielli</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Villano, Marilisa" sort="Villano, Marilisa" uniqKey="Villano M" first="Marilisa" last="Villano">Marilisa Villano</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Messina, Mario" sort="Messina, Mario" uniqKey="Messina M" first="Mario" last="Messina">Mario Messina</name>
<affiliation><nlm:aff id="au2"><institution>Department of Pediatrics, Obstetrics and Reproductive Medicine, Pediatric Surgery Section, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ferrara, Francesco" sort="Ferrara, Francesco" uniqKey="Ferrara F" first="Francesco" last="Ferrara">Francesco Ferrara</name>
<affiliation><nlm:aff id="au2"><institution>Department of Pediatrics, Obstetrics and Reproductive Medicine, Pediatric Surgery Section, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Weber, Elisabetta" sort="Weber, Elisabetta" uniqKey="Weber E" first="Elisabetta" last="Weber">Elisabetta Weber</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21039476</idno>
<idno type="pmc">3039183</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039183</idno>
<idno type="RBID">PMC:3039183</idno>
<idno type="doi">10.1111/j.1469-7580.2010.01306.x</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">002E84</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002E84</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Human microvascular lymphatic and blood endothelial cells produce fibrillin: deposition patterns and quantitative analysis</title>
<author><name sortKey="Rossi, Antonella" sort="Rossi, Antonella" uniqKey="Rossi A" first="Antonella" last="Rossi">Antonella Rossi</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gabbrielli, Erica" sort="Gabbrielli, Erica" uniqKey="Gabbrielli E" first="Erica" last="Gabbrielli">Erica Gabbrielli</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Villano, Marilisa" sort="Villano, Marilisa" uniqKey="Villano M" first="Marilisa" last="Villano">Marilisa Villano</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Messina, Mario" sort="Messina, Mario" uniqKey="Messina M" first="Mario" last="Messina">Mario Messina</name>
<affiliation><nlm:aff id="au2"><institution>Department of Pediatrics, Obstetrics and Reproductive Medicine, Pediatric Surgery Section, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ferrara, Francesco" sort="Ferrara, Francesco" uniqKey="Ferrara F" first="Francesco" last="Ferrara">Francesco Ferrara</name>
<affiliation><nlm:aff id="au2"><institution>Department of Pediatrics, Obstetrics and Reproductive Medicine, Pediatric Surgery Section, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Weber, Elisabetta" sort="Weber, Elisabetta" uniqKey="Weber E" first="Elisabetta" last="Weber">Elisabetta Weber</name>
<affiliation><nlm:aff id="au1"><institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Anatomy</title>
<idno type="ISSN">0021-8782</idno>
<idno type="eISSN">1469-7580</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Fibrillin microfibrils constitute a scaffold for elastin deposition in the wall of arteries and form the anchoring filaments that connect the lymphatic endothelium to surrounding elastic fibers. We previously reported that fibrillin is deposited in a honeycomb pattern in bovine arterial endothelial cells, which also deposit microfibril-associated glycoprotein (MAGP)-1, whereas thoracic duct endothelial cells form an irregular web. The present immunohistochemical study was designed to verify whether lymphatic and blood human dermal microvascular endothelial cells (HDMECs) isolated from human foreskin by the sequential use of a pan-endothelial marker, CD31, and the lymphatic specific marker, D2-40, deposit fibrillin and MAGP-1. In both cell types, fibrillin and MAGP-1 co-localized and were deposited with different patterns of increasing complexity co-existing in the same culture. Fibrillin microfibrils formed a wide-mesh honeycomb leaving fibrillin-free spaces that were gradually filled. This modality of fibrillin deposition, similar to that of bovine large artery endothelial cells, was basically the same in blood and lymphatic HDMECs. In some lymphatic HDMECs, fibrillin was initially deposited as uniformly scattered short fibrillin strands probably as a result of anchoring filaments carried over from the vessels of origin. Our findings show that blood and lymphatic endothelial cells participate in fibrillin deposition in human skin.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Anat</journal-id>
<journal-id journal-id-type="publisher-id">joa</journal-id>
<journal-title-group><journal-title>Journal of Anatomy</journal-title>
</journal-title-group>
<issn pub-type="ppub">0021-8782</issn>
<issn pub-type="epub">1469-7580</issn>
<publisher><publisher-name>Blackwell Science Inc</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21039476</article-id>
<article-id pub-id-type="pmc">3039183</article-id>
<article-id pub-id-type="doi">10.1111/j.1469-7580.2010.01306.x</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Human microvascular lymphatic and blood endothelial cells produce fibrillin: deposition patterns and quantitative analysis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Rossi</surname>
<given-names>Antonella</given-names>
</name>
<xref ref-type="aff" rid="au1">1</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gabbrielli</surname>
<given-names>Erica</given-names>
</name>
<xref ref-type="aff" rid="au1">1</xref>
<xref ref-type="author-notes" rid="fn1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Villano</surname>
<given-names>Marilisa</given-names>
</name>
<xref ref-type="aff" rid="au1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Messina</surname>
<given-names>Mario</given-names>
</name>
<xref ref-type="aff" rid="au2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ferrara</surname>
<given-names>Francesco</given-names>
</name>
<xref ref-type="aff" rid="au2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Weber</surname>
<given-names>Elisabetta</given-names>
</name>
<xref ref-type="aff" rid="au1">1</xref>
</contrib>
<aff id="au1"><label>1</label>
<institution>Molecular Medicine Section, Department of Neuroscience, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</aff>
<aff id="au2"><label>2</label>
<institution>Department of Pediatrics, Obstetrics and Reproductive Medicine, Pediatric Surgery Section, University of Siena</institution>
<addr-line>Siena, Italy</addr-line>
</aff>
</contrib-group>
<author-notes><corresp id="cor1">Prof. Elisabetta Weber, Dipartimento di Neuroscienze, Sezione di Medicina Molecolare, Università di Siena, Via Aldo Moro, I 53100 Siena, Italy. T: 0039 0577 234083; F: 0039 0577 234191; E: <email>weber@unisi.it</email>
</corresp>
<fn id="fn1"><label>*</label>
<p>These authors contributed equally to this work.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>12</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>11</day>
<month>10</month>
<year>2010</year>
</pub-date>
<volume>217</volume>
<issue>6</issue>
<fpage>705</fpage>
<lpage>714</lpage>
<history><date date-type="accepted"><day>10</day>
<month>9</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>Journal of Anatomy © 2010 Anatomical Society of Great Britain and Ireland</copyright-statement>
</permissions>
<abstract><p>Fibrillin microfibrils constitute a scaffold for elastin deposition in the wall of arteries and form the anchoring filaments that connect the lymphatic endothelium to surrounding elastic fibers. We previously reported that fibrillin is deposited in a honeycomb pattern in bovine arterial endothelial cells, which also deposit microfibril-associated glycoprotein (MAGP)-1, whereas thoracic duct endothelial cells form an irregular web. The present immunohistochemical study was designed to verify whether lymphatic and blood human dermal microvascular endothelial cells (HDMECs) isolated from human foreskin by the sequential use of a pan-endothelial marker, CD31, and the lymphatic specific marker, D2-40, deposit fibrillin and MAGP-1. In both cell types, fibrillin and MAGP-1 co-localized and were deposited with different patterns of increasing complexity co-existing in the same culture. Fibrillin microfibrils formed a wide-mesh honeycomb leaving fibrillin-free spaces that were gradually filled. This modality of fibrillin deposition, similar to that of bovine large artery endothelial cells, was basically the same in blood and lymphatic HDMECs. In some lymphatic HDMECs, fibrillin was initially deposited as uniformly scattered short fibrillin strands probably as a result of anchoring filaments carried over from the vessels of origin. Our findings show that blood and lymphatic endothelial cells participate in fibrillin deposition in human skin.</p>
</abstract>
<kwd-group><kwd>D2-40</kwd>
<kwd>fibrillin</kwd>
<kwd>immunohistochemistry</kwd>
<kwd>lymphatic vessels</kwd>
<kwd>microfibril-associated glycoprotein-1</kwd>
<kwd>microvascular endothelial cells</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E84 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 002E84 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Pmc |étape= Corpus |type= RBID |clé= PMC:3039183 |texte= Human microvascular lymphatic and blood endothelial cells produce fibrillin: deposition patterns and quantitative analysis }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:21039476" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
![]() | This area was generated with Dilib version V0.6.31. | ![]() |